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Gene information
General information
TC014266 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 225305..234463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014266
    iBeetle screen iB_02266
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 2

    antenna orientation irregular - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014266 RA
      MRHTVISFT LFATLLQLS KSAPSGSSA LLYLSQYGY 
      LGGNLRSLN SSALTDERV LRKAVEDFQ SFAGLDVTG 
      ELDDRTLKE MQLPRCGVK DKVGTGDNR AKRYALQGS 
      RWKVKNLNY KISKYPKNL NTKEVDKEI HRAFSVWSQ 
      YTDLTFTPS KGSAHIEIR FESGEHGDG DPFDGPGGT 
      LAHAYFPVF GGDAHFDAS EKWTINSYR GTNLFQVAA 
      HEFGHSLGL SHSDVREAL MAPFYRGYD PLFELHEDD 
      IQGIQALYG KKTRKPGGG GGGYDDSDF QGSNPGGHR 
      VPAPAPTPV DSNLCKNPK IDTIFNSAE GYTYIFKGD 
      KYWKLTEES VAPGYPKAI SSGWPGLPG DIDAAFTYK 
      NGKTYFFKG SKYWRYKGR KVDGDYPKE ISEGFTGIP 
      DDLDAAMVW SGNGKIYFF KGAKFWRFD PSQRPPVKS 
      TYPKPISNW EGVPNNLDA AFKWTNGYT YFYKGDAYY 
      RFNDRAFAV DKASPAFPR AIAYWWLGC SNAPQGTIG 
      TSESRGWLL DEESDQDYA GSDTLDTGE HHEQTPVPD 
      EDVSSPQFY QREGGNGSG VAKPSLIPL VLMLVYVLL 
      RQNVG
    CDS sequence
    • >TC014266 RA
      atgaggcac acagtgatc agttttacc ctctttgcc 
      acacttctc cagctttcg aaaagcgcg cccagtgga 
      agctctgca ctcctctat ttgtctcag tacggttac 
      ttgggcggt aacttgcga agccttaat tccagtgcc 
      ctaacggac gagcgcgtt ttgcgtaaa gccgtggag 
      gatttccag agttttgcc ggtctcgat gtcacaggc 
      gagctggac gatcgaact ctcaaagaa atgcaactt 
      ccacgatgt ggcgtcaaa gacaaagtc gggaccgga 
      gacaaccgt gccaagcgg tacgctcta cagggtagc 
      cgatggaag gtcaaaaat ctaaactac aagatctcg 
      aagtacccc aaaaatctc aacacgaag gaagtggac 
      aaggagatc cacagggcg ttctcggtc tggtcgcag 
      tacaccgac ctgaccttc accccctcc aagggcagc 
      gcccacatt gagatcaga ttcgaatcc ggggagcac 
      ggcgatggg gacccgttc gatggtcct gggggcacc 
      ctggcccac gcttacttt ccggtgttc gggggcgac 
      gcccatttc gacgcttcg gaaaagtgg accattaac 
      agctaccgc ggcaccaac ctcttccaa gtggccgcc 
      cacgaattc ggccactcg ctcggtttg agtcactcg 
      gacgtgcgg gaggcactc atggcaccg ttctaccgc 
      ggctatgac ccgcttttc gagctccac gaagacgac 
      atccagggg atccaggct ttgtacggg aagaaaacc 
      cggaaacct ggaggtggg ggtggtggt tacgacgat 
      tcggacttc caagggagc aacccgggg ggtcacagg 
      gtcccggcg ccggccccc actccggtc gattccaac 
      ttgtgcaag aatccgaag attgacacg attttcaat 
      tcggccgaa ggctacact tacatcttc aaaggggac 
      aagtactgg aagttgacc gaggagagc gtggctccg 
      gggtacccc aaagccatc tccagcggc tggcccgga 
      cttcccgga gacatagac gcggcgttc acttacaaa 
      aacggcaag acgtacttc ttcaaaggc tcgaagtac 
      tggaggtac aaggggcgg aaggtggac ggggactac 
      ccgaaggaa atttccgag ggttttact ggaattccg 
      gatgacttg gacgccgcg atggtgtgg agcggcaac 
      ggcaagatc tacttcttc aagggtgcg aagttctgg 
      cggtttgac ccgtcacag agacctcca gtcaagtcc 
      acctatcct aaaccgatc tctaattgg gaaggagtt 
      cctaataac ttagatgct gcctttaag tggaccaac 
      ggttacact tacttctac aaaggcgat gcctattat 
      cgatttaac gatagggcc tttgctgtg gacaaggcc 
      agtccggcc ttccccagg gcgatagcc tactggtgg 
      ttggggtgc agcaacgca ccgcaagga accatcgga 
      acgagtgag tccaggggg tggctcctt gatgaagag 
      agcgatcag gattacgcg ggaagtgat actttagac 
      acaggtgag caccatgag caaactcca gtgcctgat 
      gaggacgtg tccagtcct cagttttac cagcgagag 
      ggcggaaat ggaagtggt gtagccaaa ccatcccta 
      ataccctta gttttgatg ttagtgtac gttcttctt 
      cggcaaaat gtaggataa
    mRNA sequence
    • >TC014266 RA
      agtattatc tcgcgcgtc gcgagttcc accgatatc 
      acaaccgac gaccaaatt ggacgtttc actaattta 
      taaacgcat attagttag atgatagaa ccagtttgg 
      tcttgtgat ttttcgtcg agcccaaaa ttttgtcaa 
      aaagcacaa aactcgcga atgacgacg cagtgcaat 
      ccgataagg agataacgc gaaaacaat tagttcaaa 
      atgaggcac acagtgatc agttttacc ctctttgcc 
      acacttctc cagctttcg aaaagcgcg cccagtgga 
      agctctgca ctcctctat ttgtctcag tacggttac 
      ttgggcggt aacttgcga agccttaat tccagtgcc 
      ctaacggac gagcgcgtt ttgcgtaaa gccgtggag 
      gatttccag agttttgcc ggtctcgat gtcacaggc 
      gagctggac gatcgaact ctcaaagaa atgcaactt 
      ccacgatgt ggcgtcaaa gacaaagtc gggaccgga 
      gacaaccgt gccaagcgg tacgctcta cagggtagc 
      cgatggaag gtcaaaaat ctaaactac aagatctcg 
      aagtacccc aaaaatctc aacacgaag gaagtggac 
      aaggagatc cacagggcg ttctcggtc tggtcgcag 
      tacaccgac ctgaccttc accccctcc aagggcagc 
      gcccacatt gagatcaga ttcgaatcc ggggagcac 
      ggcgatggg gacccgttc gatggtcct gggggcacc 
      ctggcccac gcttacttt ccggtgttc gggggcgac 
      gcccatttc gacgcttcg gaaaagtgg accattaac 
      agctaccgc ggcaccaac ctcttccaa gtggccgcc 
      cacgaattc ggccactcg ctcggtttg agtcactcg 
      gacgtgcgg gaggcactc atggcaccg ttctaccgc 
      ggctatgac ccgcttttc gagctccac gaagacgac 
      atccagggg atccaggct ttgtacggg aagaaaacc 
      cggaaacct ggaggtggg ggtggtggt tacgacgat 
      tcggacttc caagggagc aacccgggg ggtcacagg 
      gtcccggcg ccggccccc actccggtc gattccaac 
      ttgtgcaag aatccgaag attgacacg attttcaat 
      tcggccgaa ggctacact tacatcttc aaaggggac 
      aagtactgg aagttgacc gaggagagc gtggctccg 
      gggtacccc aaagccatc tccagcggc tggcccgga 
      cttcccgga gacatagac gcggcgttc acttacaaa 
      aacggcaag acgtacttc ttcaaaggc tcgaagtac 
      tggaggtac aaggggcgg aaggtggac ggggactac 
      ccgaaggaa atttccgag ggttttact ggaattccg 
      gatgacttg gacgccgcg atggtgtgg agcggcaac 
      ggcaagatc tacttcttc aagggtgcg aagttctgg 
      cggtttgac ccgtcacag agacctcca gtcaagtcc 
      acctatcct aaaccgatc tctaattgg gaaggagtt 
      cctaataac ttagatgct gcctttaag tggaccaac 
      ggttacact tacttctac aaaggcgat gcctattat 
      cgatttaac gatagggcc tttgctgtg gacaaggcc 
      agtccggcc ttccccagg gcgatagcc tactggtgg 
      ttggggtgc agcaacgca ccgcaagga accatcgga 
      acgagtgag tccaggggg tggctcctt gatgaagag 
      agcgatcag gattacgcg ggaagtgat actttagac 
      acaggtgag caccatgag caaactcca gtgcctgat 
      gaggacgtg tccagtcct cagttttac cagcgagag 
      ggcggaaat ggaagtggt gtagccaaa ccatcccta 
      ataccctta gttttgatg ttagtgtac gttcttctt 
      cggcaaaat gtaggataa attgagaat atttcgttt 
      tataaacga gacagctca gactgagac attaaacta 
      atttactgt tttgtgtgt acatatcgg tgaaacttt 
      cgcaacggc gcaaaattt gacaaagtg catctatac 
      atttcattc ataatttat tgaagccag tagatcgta 
      ttttttata atgctcgtt gtactaaac ctagcgata 
      atactgtgc aattgtgat tcgttgttg ttgattcaa 
      aaagttcac ggaacgcaa atcttgtaa atgttcttt 
      taactttta agtaataaa atacctaaa agtaa