Status
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Gene information
General information
TC014250 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7964199..7975855
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014250
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02265

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 50
    head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014250 RA
      MADVPVRVE ERLKIKIGE AKQLQPRSN LSSVARDVY 
CILSLDQEE IYRTKTAEK TLNPFFGEE YQFEVPRDF 
RFISIYLFD RDKHLKQNK VLGKVAIKR EELSSYNNR 
DHWFAIKPV DADSEVQGK VNIELKFSS SHHKSKWVN 
CYSRNDRLS VQVVKCLDL TLKNGACDP YAVACVTYT 
NRKTVSKRT KVRKKTTCP QFDEIFIFD GYEGRDKDM 
CGEEVEICE LNVSIWHDA PGMGDDVFL GEVRVQLSG 
IQQQNAAPR NAWYFLQPR SAKNRPSFT YSTPPGTRL 
SKDNSLGSL RLQVQYNED RVFPAEIYE SLKHLLLKS 
VDTKPITSS AVYVLGEII SSKMEIAQP LVRIFMHHG 
QIVPIIKAL ADAEISMLT DPTTIFRGN TLVSKMMDE 
AMRLIGLQY LHKTLRPTI ELIFSERKP CEIDPTRVR 
DSNTVQTNL NNLKTYVLK VFKAITQSA IHCPALMCQ 
IFYNLKECA MKRFPENKE VRYSVISGF IFLRFFAPA 
ILGPRLFDL TNEQIDSQI NRTLTLISK TIQSLGNLV 
SCRSSQQVC KEEYMESLY KEFYTETHV TAVRQFLEI 
ISAISNPEN RDNDTPVTL KEGLMIKRA QGRKRFGRK 
NFKQRYFKL TTHNLSYAK SKGKEALCQ IPLSNILAV 
ERLTEKSFK MKNMFQIVQ DGRTLYVQA ANCVEEKEW 
VDLLTKMCQ NNQNRLNQY HPSAFINGR WSCCESPNE 
LAPGCSKVS SNDSNNFHI TLDPERELQ RIHSLIMSH 
MNQLELIMC QPEAFMKDI LQFKTYGNH DVKQFFKVV 
SELRDVAAK IDAKHKTYM RTLARDTKY GSLAAPIGD 
DNYLLASRM NLDSLLSRR
    CDS sequence
    • >TC014250 RA
      atggcggac gtcccagtg agagtcgag gaaaggctc 
aaaattaaa atcggagag gccaagcag ctgcaaccc 
agatcaaac ctgtcttcg gttgcacga gatgtttac 
tgcatcctc tccctggac caggaggag atttacaga 
accaagacg gctgagaaa actttgaac ccctttttc 
ggcgaggag taccagttc gaggtcccc cgggacttc 
cgcttcatc tcgatctac ctcttcgac cgagacaag 
cacctcaaa cagaacaag gtcctgggc aaggtggcc 
ataaagcgc gaggaactc tcctcgtac aacaaccgt 
gaccattgg ttcgccata aagcccgtg gatgcggac 
tcggaggtg cagggcaaa gtcaatatt gagctgaaa 
ttctcctcc agccaccac aagagcaaa tgggtgaat 
tgctactcc cgcaacgac cgtctgtcg gtgcaagtg 
gtgaagtgt ttagatctg acgttgaag aacggtgct 
tgtgatccg tacgctgtg gcctgtgtg acttacacg 
aaccgtaag acggtgtca aagaggacg aaagtgagg 
aaaaaaacg acgtgtccg cagttcgac gagattttt 
atttttgac ggatacgag gggcgggat aaggatatg 
tgcggggag gaggtggag atctgtgag ttgaacgtg 
tcgatctgg cacgacgcc cccggaatg ggggatgac 
gtgtttttg ggggaggtg agggtgcag ctgagcggg 
atacagcag cagaatgcg gcgccgagg aatgcctgg 
tatttcctt cagcccaga tcggcgaaa aatcgtccg 
tcctttacg tactcgacg ccacccggt acaagattg 
tccaaagat aattctctg ggttcgctc agactccaa 
gtgcagtac aatgaggac agggtcttt cctgccgaa 
atatacgag tctttgaag catctgttg ctgaaaagt 
gtcgacacg aagccgatt acttcaagt gctgtatac 
gttttgggc gaaatcata tcaagcaag atggaaatt 
gcccaacct ttggttagg atatttatg catcatggt 
caaattgtt cccatcatc aaggctttg gccgacgcc 
gaaatttcg atgttgacg gatccgact acaatattc 
cgcggtaat actctcgtt tcgaaaatg atggatgaa 
gcgatgcgt ctcatcggt ctccagtat ctgcacaag 
actttacgg ccgacaatt gagctcatt tttagtgaa 
agaaaaccg tgcgaaatc gatccaacg cgcgtccgt 
gattccaat accgtccaa acaaatctc aacaatctc 
aaaacttac gtccttaaa gtttttaaa gcaataact 
caaagcgcg attcattgt ccagctctc atgtgccag 
atattttac aatttgaag gaatgtgcg atgaaacgg 
tttccggaa aacaaggaa gttcgatat tcggtcatt 
tcggggttc atttttttg aggtttttt gcaccggcg 
atattagga ccacgactt tttgacctg acgaatgaa 
caaatcgat agtcaaatc aatcggacg ttgacactg 
atttcaaaa accatccag tcgttgggc aatcttgtg 
agttgcagg tcgagtcaa caagtttgc aaagaggaa 
tatatggag tcattgtat aaagaattt tacacagag 
actcacgtt acagccgtt cggcagttt ttggaaatt 
atttcggcg atttcgaat ccggaaaat cgcgataac 
gacacacct gtgacttta aaagaggga ctgatgata 
aagcgtgct cagggccgg aaaaggttc ggtaggaag 
aacttcaag cagcgctat ttcaaattg acgactcac 
aatttgagt tacgctaaa agcaaaggc aaggaggct 
ctttgtcag atacctttg tcgaatatt ttggctgtg 
gaaaggcta acggagaag tcgtttaaa atgaaaaac 
atgttccaa atcgttcaa gacggacgt actttgtac 
gtgcaagcg gccaattgt gtcgaggag aaagagtgg 
gtggatttg ctcacgaaa atgtgccag aataaccaa 
aaccggctg aatcaatat catccgtcc gcttttatc 
aacgggagg tggtcatgt tgtgaatct ccgaacgag 
ttggcgcca ggttgcagc aaagtgtcg tcaaacgat 
agcaataat tttcacata accttggat ccagagcgg 
gagttgcaa aggattcat tcgttgatt atgtcgcat 
atgaatcaa ctggagttg atcatgtgc cagcctgag 
gcgtttatg aaggatatt ttacagttt aaaacgtac 
gggaaccac gacgtcaag cagtttttt aaagttgtg 
agtgagctg cgagatgtc gctgcgaaa attgatgcc 
aaacacaag acatatatg cggacctta gccagagat 
acgaaatac ggcagctta gctgcgccc ataggtgac 
gataattat ttattggcg tcgaggatg aatcttgat 
tcgttgtta tcaaggcga taa
    mRNA sequence
    • >TC014250 RA
      ctggaatcc ctctagtga ataattcca cgtgctagc 
accgtcatg aaatttgta ttaattaat cgcaatgga 
tgagttatg ggaacatac ggtgtgctt tttctgttc 
attcataca ctcggaact tgcgtagtg cgctctttg 
cgccacttt gtaacgatt tgtgagtgc agagctcgc 
aatcccatt cagttcatt cataaaacc cggtggtga 
ataacagca cgtgctgga cagccccat tcacaaccc 
tcgaaaatg gcgcctttt ttcgtgcca cactcctga 
agttggcta cgcagctgg gcgacttga tgcggtcca 
ctgtctgta ctcatgtca aaccgatcc attcatttt 
ttcaataga acatggagt agacagaaa catggcgga 
cgtcccagt gagagtcga ggaaaggct caaaattaa 
aatcggaga ggccaagca gctgcaacc cagatcaaa 
cctgtcttc ggttgcacg agatgttta ctgcatcct 
ctccctgga ccaggagga gatttacag aaccaagac 
ggctgagaa aactttgaa ccccttttt cggcgagga 
gtaccagtt cgaggtccc ccgggactt ccgcttcat 
ctcgatcta cctcttcga ccgagacaa gcacctcaa 
acagaacaa ggtcctggg caaggtggc cataaagcg 
cgaggaact ctcctcgta caacaaccg tgaccattg 
gttcgccat aaagcccgt ggatgcgga ctcggaggt 
gcagggcaa agtcaatat tgagctgaa attctcctc 
cagccacca caagagcaa atgggtgaa ttgctactc 
ccgcaacga ccgtctgtc ggtgcaagt ggtgaagtg 
tttagatct gacgttgaa gaacggtgc ttgtgatcc 
gtacgctgt ggcctgtgt gacttacac gaaccgtaa 
gacggtgtc aaagaggac gaaagtgag gaaaaaaac 
gacgtgtcc gcagttcga cgagatttt tatttttga 
cggatacga ggggcggga taaggatat gtgcgggga 
ggaggtgga gatctgtga gttgaacgt gtcgatctg 
gcacgacgc ccccggaat gggggatga cgtgttttt 
gggggaggt gagggtgca gctgagcgg gatacagca 
gcagaatgc ggcgccgag gaatgcctg gtatttcct 
tcagcccag atcggcgaa aaatcgtcc gtcctttac 
gtactcgac gccacccgg tacaagatt gtccaaaga 
taattctct gggttcgct cagactcca agtgcagta 
caatgagga cagggtctt tcctgccga aatatacga 
gtctttgaa gcatctgtt gctgaaaag tgtcgacac 
gaagccgat tacttcaag tgctgtata cgttttggg 
cgaaatcat atcaagcaa gatggaaat tgcccaacc 
tttggttag gatatttat gcatcatgg tcaaattgt 
tcccatcat caaggcttt ggccgacgc cgaaatttc 
gatgttgac ggatccgac tacaatatt ccgcggtaa 
tactctcgt ttcgaaaat gatggatga agcgatgcg 
tctcatcgg tctccagta tctgcacaa gactttacg 
gccgacaat tgagctcat ttttagtga aagaaaacc 
gtgcgaaat cgatccaac gcgcgtccg tgattccaa 
taccgtcca aacaaatct caacaatct caaaactta 
cgtccttaa agtttttaa agcaataac tcaaagcgc 
gattcattg tccagctct catgtgcca gatatttta 
caatttgaa ggaatgtgc gatgaaacg gtttccgga 
aaacaagga agttcgata ttcggtcat ttcggggtt 
cattttttt gaggttttt tgcaccggc gatattagg 
accacgact ttttgacct gacgaatga acaaatcga 
tagtcaaat caatcggac gttgacact gatttcaaa 
aaccatcca gtcgttggg caatcttgt gagttgcag 
gtcgagtca acaagtttg caaagagga atatatgga 
gtcattgta taaagaatt ttacacaga gactcacgt 
tacagccgt tcggcagtt tttggaaat tatttcggc 
gatttcgaa tccggaaaa tcgcgataa cgacacacc 
tgtgacttt aaaagaggg actgatgat aaagcgtgc 
tcagggccg gaaaaggtt cggtaggaa gaacttcaa 
gcagcgcta tttcaaatt gacgactca caatttgag 
ttacgctaa aagcaaagg caaggaggc tctttgtca 
gataccttt gtcgaatat tttggctgt ggaaaggct 
aacggagaa gtcgtttaa aatgaaaaa catgttcca 
aatcgttca agacggacg tactttgta cgtgcaagc 
ggccaattg tgtcgagga gaaagagtg ggtggattt 
gctcacgaa aatgtgcca gaataacca aaaccggct 
gaatcaata tcatccgtc cgcttttat caacgggag 
gtggtcatg ttgtgaatc tccgaacga gttggcgcc 
aggttgcag caaagtgtc gtcaaacga tagcaataa 
ttttcacat aaccttgga tccagagcg ggagttgca 
aaggattca ttcgttgat tatgtcgca tatgaatca 
actggagtt gatcatgtg ccagcctga ggcgtttat 
gaaggatat tttacagtt taaaacgta cgggaacca 
cgacgtcaa gcagttttt taaagttgt gagtgagct 
gcgagatgt cgctgcgaa aattgatgc caaacacaa 
gacatatat gcggacctt agccagaga tacgaaata 
cggcagctt agctgcgcc cataggtga cgataatta 
tttattggc gtcgaggat gaatcttga ttcgttgtt 
atcaaggcg ataaatact tccggcact gtacaaaat 
aaccgaaaa tctcaattt gaagactgc aaaattaat 
gttaagcaa ttaaagaac accagtgta ttattctaa 
aagaagtta atcttattg aagatttta aattagctg 
gtaaggttc aaagacaat caagttatt cttagtttt 
tccaatgta tgtatattt atattgttt tttttttgt 
attaaatga gtacaaata cgttgtaaa aagcgtgtc 
ttacataaa tacatcctt ataataaat gcagggttg 
taattttag gggagtcca gatttgcgt atacgcgaa 
tatttttaa taaacgttt tatccataa tacgtggag 
caagttctt tataaggat gatattttc ggatactgt 
atagtgtaa atacttgtt agaggccat gagtgcagg 
gcatttata aattgagtt cagttatta tttgttcaa 
ctgtttgtt atccaaata acagctttt ttaatcttt 
tttaatttt atattaatg acgttagct tagtgtttt 
tccttgtcc gtttttttg atgtatagc ccaaatttt 
aaagcagtc taacttgac ataataccg cttagtagc 
tataaactc catatagaa attaggagg aaaattgta 
aatttcgtt ttatattct gtaaagtgg tgtttttta 
atataattt gcatttaat gtattttta atgattatc 
gtttttatt aacttatta acattcttg caaaatcgt 
ttaaactgt tcacgttga tttaagtgt tacaaagtt 
taggactat tccaataaa ttctatgtt tattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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