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Gene information
General information
TC014250 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 48785..60441
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014250
    iBeetle screen iB_02265
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 50

    head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014250 RA
      MADVPVRVE ERLKIKIGE AKQLQPRSN LSSVARDVY 
      CILSLDQEE IYRTKTAEK TLNPFFGEE YQFEVPRDF 
      RFISIYLFD RDKHLKQNK VLGKVAIKR EELSSYNNR 
      DHWFAIKPV DADSEVQGK VNIELKFSS SHHKSKWVN 
      CYSRNDRLS VQVVKCLDL TLKNGACDP YAVACVTYT 
      NRKTVSKRT KVRKKTTCP QFDEIFIFD GYEGRDKDM 
      CGEEVEICE LNVSIWHDA PGMGDDVFL GEVRVQLSG 
      IQQQNAAPR NAWYFLQPR SAKNRPSFT YSTPPGTRL 
      SKDNSLGSL RLQVQYNED RVFPAEIYE SLKHLLLKS 
      VDTKPITSS AVYVLGEII SSKMEIAQP LVRIFMHHG 
      QIVPIIKAL ADAEISMLT DPTTIFRGN TLVSKMMDE 
      AMRLIGLQY LHKTLRPTI ELIFSERKP CEIDPTRVR 
      DSNTVQTNL NNLKTYVLK VFKAITQSA IHCPALMCQ 
      IFYNLKECA MKRFPENKE VRYSVISGF IFLRFFAPA 
      ILGPRLFDL TNEQIDSQI NRTLTLISK TIQSLGNLV 
      SCRSSQQVC KEEYMESLY KEFYTETHV TAVRQFLEI 
      ISAISNPEN RDNDTPVTL KEGLMIKRA QGRKRFGRK 
      NFKQRYFKL TTHNLSYAK SKGKEALCQ IPLSNILAV 
      ERLTEKSFK MKNMFQIVQ DGRTLYVQA ANCVEEKEW 
      VDLLTKMCQ NNQNRLNQY HPSAFINGR WSCCESPNE 
      LAPGCSKVS SNDSNNFHI TLDPERELQ RIHSLIMSH 
      MNQLELIMC QPEAFMKDI LQFKTYGNH DVKQFFKVV 
      SELRDVAAK IDAKHKTYM RTLARDTKY GSLAAPIGD 
      DNYLLASRM NLDSLLSRR
    CDS sequence
    • >TC014250 RA
      atggcggac gtcccagtg agagtcgag gaaaggctc 
      aaaattaaa atcggagag gccaagcag ctgcaaccc 
      agatcaaac ctgtcttcg gttgcacga gatgtttac 
      tgcatcctc tccctggac caggaggag atttacaga 
      accaagacg gctgagaaa actttgaac ccctttttc 
      ggcgaggag taccagttc gaggtcccc cgggacttc 
      cgcttcatc tcgatctac ctcttcgac cgagacaag 
      cacctcaaa cagaacaag gtcctgggc aaggtggcc 
      ataaagcgc gaggaactc tcctcgtac aacaaccgt 
      gaccattgg ttcgccata aagcccgtg gatgcggac 
      tcggaggtg cagggcaaa gtcaatatt gagctgaaa 
      ttctcctcc agccaccac aagagcaaa tgggtgaat 
      tgctactcc cgcaacgac cgtctgtcg gtgcaagtg 
      gtgaagtgt ttagatctg acgttgaag aacggtgct 
      tgtgatccg tacgctgtg gcctgtgtg acttacacg 
      aaccgtaag acggtgtca aagaggacg aaagtgagg 
      aaaaaaacg acgtgtccg cagttcgac gagattttt 
      atttttgac ggatacgag gggcgggat aaggatatg 
      tgcggggag gaggtggag atctgtgag ttgaacgtg 
      tcgatctgg cacgacgcc cccggaatg ggggatgac 
      gtgtttttg ggggaggtg agggtgcag ctgagcggg 
      atacagcag cagaatgcg gcgccgagg aatgcctgg 
      tatttcctt cagcccaga tcggcgaaa aatcgtccg 
      tcctttacg tactcgacg ccacccggt acaagattg 
      tccaaagat aattctctg ggttcgctc agactccaa 
      gtgcagtac aatgaggac agggtcttt cctgccgaa 
      atatacgag tctttgaag catctgttg ctgaaaagt 
      gtcgacacg aagccgatt acttcaagt gctgtatac 
      gttttgggc gaaatcata tcaagcaag atggaaatt 
      gcccaacct ttggttagg atatttatg catcatggt 
      caaattgtt cccatcatc aaggctttg gccgacgcc 
      gaaatttcg atgttgacg gatccgact acaatattc 
      cgcggtaat actctcgtt tcgaaaatg atggatgaa 
      gcgatgcgt ctcatcggt ctccagtat ctgcacaag 
      actttacgg ccgacaatt gagctcatt tttagtgaa 
      agaaaaccg tgcgaaatc gatccaacg cgcgtccgt 
      gattccaat accgtccaa acaaatctc aacaatctc 
      aaaacttac gtccttaaa gtttttaaa gcaataact 
      caaagcgcg attcattgt ccagctctc atgtgccag 
      atattttac aatttgaag gaatgtgcg atgaaacgg 
      tttccggaa aacaaggaa gttcgatat tcggtcatt 
      tcggggttc atttttttg aggtttttt gcaccggcg 
      atattagga ccacgactt tttgacctg acgaatgaa 
      caaatcgat agtcaaatc aatcggacg ttgacactg 
      atttcaaaa accatccag tcgttgggc aatcttgtg 
      agttgcagg tcgagtcaa caagtttgc aaagaggaa 
      tatatggag tcattgtat aaagaattt tacacagag 
      actcacgtt acagccgtt cggcagttt ttggaaatt 
      atttcggcg atttcgaat ccggaaaat cgcgataac 
      gacacacct gtgacttta aaagaggga ctgatgata 
      aagcgtgct cagggccgg aaaaggttc ggtaggaag 
      aacttcaag cagcgctat ttcaaattg acgactcac 
      aatttgagt tacgctaaa agcaaaggc aaggaggct 
      ctttgtcag atacctttg tcgaatatt ttggctgtg 
      gaaaggcta acggagaag tcgtttaaa atgaaaaac 
      atgttccaa atcgttcaa gacggacgt actttgtac 
      gtgcaagcg gccaattgt gtcgaggag aaagagtgg 
      gtggatttg ctcacgaaa atgtgccag aataaccaa 
      aaccggctg aatcaatat catccgtcc gcttttatc 
      aacgggagg tggtcatgt tgtgaatct ccgaacgag 
      ttggcgcca ggttgcagc aaagtgtcg tcaaacgat 
      agcaataat tttcacata accttggat ccagagcgg 
      gagttgcaa aggattcat tcgttgatt atgtcgcat 
      atgaatcaa ctggagttg atcatgtgc cagcctgag 
      gcgtttatg aaggatatt ttacagttt aaaacgtac 
      gggaaccac gacgtcaag cagtttttt aaagttgtg 
      agtgagctg cgagatgtc gctgcgaaa attgatgcc 
      aaacacaag acatatatg cggacctta gccagagat 
      acgaaatac ggcagctta gctgcgccc ataggtgac 
      gataattat ttattggcg tcgaggatg aatcttgat 
      tcgttgtta tcaaggcga taa
    mRNA sequence
    • >TC014250 RA
      ctggaatcc ctctagtga ataattcca cgtgctagc 
      accgtcatg aaatttgta ttaattaat cgcaatgga 
      tgagttatg ggaacatac ggtgtgctt tttctgttc 
      attcataca ctcggaact tgcgtagtg cgctctttg 
      cgccacttt gtaacgatt tgtgagtgc agagctcgc 
      aatcccatt cagttcatt cataaaacc cggtggtga 
      ataacagca cgtgctgga cagccccat tcacaaccc 
      tcgaaaatg gcgcctttt ttcgtgcca cactcctga 
      agttggcta cgcagctgg gcgacttga tgcggtcca 
      ctgtctgta ctcatgtca aaccgatcc attcatttt 
      ttcaataga acatggagt agacagaaa catggcgga 
      cgtcccagt gagagtcga ggaaaggct caaaattaa 
      aatcggaga ggccaagca gctgcaacc cagatcaaa 
      cctgtcttc ggttgcacg agatgttta ctgcatcct 
      ctccctgga ccaggagga gatttacag aaccaagac 
      ggctgagaa aactttgaa ccccttttt cggcgagga 
      gtaccagtt cgaggtccc ccgggactt ccgcttcat 
      ctcgatcta cctcttcga ccgagacaa gcacctcaa 
      acagaacaa ggtcctggg caaggtggc cataaagcg 
      cgaggaact ctcctcgta caacaaccg tgaccattg 
      gttcgccat aaagcccgt ggatgcgga ctcggaggt 
      gcagggcaa agtcaatat tgagctgaa attctcctc 
      cagccacca caagagcaa atgggtgaa ttgctactc 
      ccgcaacga ccgtctgtc ggtgcaagt ggtgaagtg 
      tttagatct gacgttgaa gaacggtgc ttgtgatcc 
      gtacgctgt ggcctgtgt gacttacac gaaccgtaa 
      gacggtgtc aaagaggac gaaagtgag gaaaaaaac 
      gacgtgtcc gcagttcga cgagatttt tatttttga 
      cggatacga ggggcggga taaggatat gtgcgggga 
      ggaggtgga gatctgtga gttgaacgt gtcgatctg 
      gcacgacgc ccccggaat gggggatga cgtgttttt 
      gggggaggt gagggtgca gctgagcgg gatacagca 
      gcagaatgc ggcgccgag gaatgcctg gtatttcct 
      tcagcccag atcggcgaa aaatcgtcc gtcctttac 
      gtactcgac gccacccgg tacaagatt gtccaaaga 
      taattctct gggttcgct cagactcca agtgcagta 
      caatgagga cagggtctt tcctgccga aatatacga 
      gtctttgaa gcatctgtt gctgaaaag tgtcgacac 
      gaagccgat tacttcaag tgctgtata cgttttggg 
      cgaaatcat atcaagcaa gatggaaat tgcccaacc 
      tttggttag gatatttat gcatcatgg tcaaattgt 
      tcccatcat caaggcttt ggccgacgc cgaaatttc 
      gatgttgac ggatccgac tacaatatt ccgcggtaa 
      tactctcgt ttcgaaaat gatggatga agcgatgcg 
      tctcatcgg tctccagta tctgcacaa gactttacg 
      gccgacaat tgagctcat ttttagtga aagaaaacc 
      gtgcgaaat cgatccaac gcgcgtccg tgattccaa 
      taccgtcca aacaaatct caacaatct caaaactta 
      cgtccttaa agtttttaa agcaataac tcaaagcgc 
      gattcattg tccagctct catgtgcca gatatttta 
      caatttgaa ggaatgtgc gatgaaacg gtttccgga 
      aaacaagga agttcgata ttcggtcat ttcggggtt 
      cattttttt gaggttttt tgcaccggc gatattagg 
      accacgact ttttgacct gacgaatga acaaatcga 
      tagtcaaat caatcggac gttgacact gatttcaaa 
      aaccatcca gtcgttggg caatcttgt gagttgcag 
      gtcgagtca acaagtttg caaagagga atatatgga 
      gtcattgta taaagaatt ttacacaga gactcacgt 
      tacagccgt tcggcagtt tttggaaat tatttcggc 
      gatttcgaa tccggaaaa tcgcgataa cgacacacc 
      tgtgacttt aaaagaggg actgatgat aaagcgtgc 
      tcagggccg gaaaaggtt cggtaggaa gaacttcaa 
      gcagcgcta tttcaaatt gacgactca caatttgag 
      ttacgctaa aagcaaagg caaggaggc tctttgtca 
      gataccttt gtcgaatat tttggctgt ggaaaggct 
      aacggagaa gtcgtttaa aatgaaaaa catgttcca 
      aatcgttca agacggacg tactttgta cgtgcaagc 
      ggccaattg tgtcgagga gaaagagtg ggtggattt 
      gctcacgaa aatgtgcca gaataacca aaaccggct 
      gaatcaata tcatccgtc cgcttttat caacgggag 
      gtggtcatg ttgtgaatc tccgaacga gttggcgcc 
      aggttgcag caaagtgtc gtcaaacga tagcaataa 
      ttttcacat aaccttgga tccagagcg ggagttgca 
      aaggattca ttcgttgat tatgtcgca tatgaatca 
      actggagtt gatcatgtg ccagcctga ggcgtttat 
      gaaggatat tttacagtt taaaacgta cgggaacca 
      cgacgtcaa gcagttttt taaagttgt gagtgagct 
      gcgagatgt cgctgcgaa aattgatgc caaacacaa 
      gacatatat gcggacctt agccagaga tacgaaata 
      cggcagctt agctgcgcc cataggtga cgataatta 
      tttattggc gtcgaggat gaatcttga ttcgttgtt 
      atcaaggcg ataaatact tccggcact gtacaaaat 
      aaccgaaaa tctcaattt gaagactgc aaaattaat 
      gttaagcaa ttaaagaac accagtgta ttattctaa 
      aagaagtta atcttattg aagatttta aattagctg 
      gtaaggttc aaagacaat caagttatt cttagtttt 
      tccaatgta tgtatattt atattgttt tttttttgt 
      attaaatga gtacaaata cgttgtaaa aagcgtgtc 
      ttacataaa tacatcctt ataataaat gcagggttg 
      taattttag gggagtcca gatttgcgt atacgcgaa 
      tatttttaa taaacgttt tatccataa tacgtggag 
      caagttctt tataaggat gatattttc ggatactgt 
      atagtgtaa atacttgtt agaggccat gagtgcagg 
      gcatttata aattgagtt cagttatta tttgttcaa 
      ctgtttgtt atccaaata acagctttt ttaatcttt 
      tttaatttt atattaatg acgttagct tagtgtttt 
      tccttgtcc gtttttttg atgtatagc ccaaatttt 
      aaagcagtc taacttgac ataataccg cttagtagc 
      tataaactc catatagaa attaggagg aaaattgta 
      aatttcgtt ttatattct gtaaagtgg tgtttttta 
      atataattt gcatttaat gtattttta atgattatc 
      gtttttatt aacttatta acattcttg caaaatcgt 
      ttaaactgt tcacgttga tttaagtgt tacaaagtt 
      taggactat tccaataaa ttctatgtt tattt