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Gene information
General information
TC014246 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7921405..7922909
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014246
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02264

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014246 RA
      MNIRPIFNT KEVLDQEIT LCDMLMACA PITVIKNDG 
      LPDRICDNC IEELRKAFS FRKMCENSD TTLRNCLNN 
      LKPVIVQTS HEIEDIKPN ISDDLVEES QIDWGDNSL 
      SGDTSNEEN LKDVENNTK TLENGRRKR SYKCKECNE 
      ECLGLASYW KHMSNQHGT KLKCEFCDK EFRTPYLLD 
      EHKSSCPGE IEPKPKRTR KRTGITKKR NLPRTCDVC 
      HKTFRFHSN LERHQLTHT GERPYLCNV CGKGFGQLS 
      YLKIHSFIH TGEKPYKCQ MCDKSFAAP GTLMTHIRI 
      HTGERPHVC KICGKDFPQ SGYLSAHIR THTGEKPVE 
      CHVCHRRFN QSGRLVIHM RIHSGEKPF SCNECGRSF 
      AVKGTLKKH IRTHTGERP YVCSVCGQA FAQSGTLAT 
      HMKVHRPKP
    CDS sequence
    • >TC014246 RA
      atgaacata agaccgatt ttcaacact aaggaagtc 
      ttagatcaa gaaataact ctgtgtgac atgctaatg 
      gcatgtgcc ccaatcacg gtaataaaa aatgacggc 
      ttgcctgat cgcatatgt gacaattgt atcgaggaa 
      ttgcgtaaa gcgttttca ttcagaaaa atgtgcgaa 
      aactcagac acaactcta cggaactgc ttgaataat 
      ctaaaacct gttatagta caaactagc catgaaatt 
      gaagacatc aagccgaac ataagcgac gatttagtc 
      gaggaaagc caaattgat tggggcgac aattcactt 
      tcaggcgac acaagtaac gaagaaaat ttaaaggac 
      gtcgaaaac aacaccaaa accctggaa aacgggagg 
      cggaagcgt tcatacaaa tgcaaggaa tgcaacgaa 
      gagtgttta gggctcgcc tcctactgg aagcacatg 
      agcaaccaa cacggaacg aagctaaaa tgcgaattt 
      tgcgacaaa gaattcagg actccgtac ctgttagac 
      gaacacaaa tcttcatgc cctggcgaa atcgagccc 
      aagccaaaa cgcacgagg aagagaacc ggaatcacc 
      aaaaaacgc aacctacct cgcacttgc gacgtctgc 
      cacaaaacc ttccgcttc cacagcaac ttggagcgc 
      caccagctc acacacacc ggggaaagg ccctacctg 
      tgcaacgtc tgcggcaaa ggcttcggc cagttgtct 
      tacctcaaa atccactcg ttcatacac accggggaa 
      aaaccgtac aagtgccaa atgtgcgac aaaagcttc 
      gcagcgccg ggaactctc atgacccac attcgcatc 
      cacacgggg gaaagaccg cacgtgtgt aaaatttgc 
      ggcaaggat ttcccacag tcggggtac ttgtcggcg 
      catattcgc acacacacc ggggaaaaa ccggtcgag 
      tgccacgtc tgccatcgc cggttcaac caaagcggc 
      cgtctggtc atacacatg aggatacac agcggggaa 
      aaaccgttc tcgtgcaac gaatgcggg aggagtttt 
      gcggtcaaa gggacgctc aagaagcac atcaggacg 
      catacgggg gaaagaccg tatgtgtgc agtgtgtgc 
      ggacaggcg ttcgcccag agcgggacc ctggccacg 
      cacatgaag gtgcacagg cccaagccg taa
    mRNA sequence
    • >TC014246 RA
      ttgttgaca aattgatgt caacatttc tctaaaaat 
      gaattgtac ggattattc cagcgtttg tagaacttg 
      tatgtctca gaatatgaa cataagacc gattttcaa 
      cactaagga agtcttaga tcaagaaat aactctgtg 
      tgacatgct aatggcatg tgccccaat cacggtaat 
      aaaaaatga cggcttgcc tgatcgcat atgtgacaa 
      ttgtatcga ggaattgcg taaagcgtt ttcattcag 
      aaaaatgtg cgaaaactc agacacaac tctacggaa 
      ctgcttgaa taatctaaa acctgttat agtacaaac 
      tagccatga aattgaaga catcaagcc gaacataag 
      cgacgattt agtcgagga aagccaaat tgattgggg 
      cgacaattc actttcagg cgacacaag taacgaaga 
      aaatttaaa ggacgtcga aaacaacac caaaaccct 
      ggaaaacgg gaggcggaa gcgttcata caaatgcaa 
      ggaatgcaa cgaagagtg tttagggct cgcctccta 
      ctggaagca catgagcaa ccaacacgg aacgaagct 
      aaaatgcga attttgcga caaagaatt caggactcc 
      gtacctgtt agacgaaca caaatcttc atgccctgg 
      cgaaatcga gcccaagcc aaaacgcac gaggaagag 
      aaccggaat caccaaaaa acgcaacct acctcgcac 
      ttgcgacgt ctgccacaa aaccttccg cttccacag 
      caacttgga gcgccacca gctcacaca caccgggga 
      aaggcccta cctgtgcaa cgtctgcgg caaaggctt 
      cggccagtt gtcttacct caaaatcca ctcgttcat 
      acacaccgg ggaaaaacc gtacaagtg ccaaatgtg 
      cgacaaaag cttcgcagc gccgggaac tctcatgac 
      ccacattcg catccacac gggggaaag accgcacgt 
      gtgtaaaat ttgcggcaa ggatttccc acagtcggg 
      gtacttgtc ggcgcatat tcgcacaca caccgggga 
      aaaaccggt cgagtgcca cgtctgcca tcgccggtt 
      caaccaaag cggccgtct ggtcataca catgaggat 
      acacagcgg ggaaaaacc gttctcgtg caacgaatg 
      cgggaggag ttttgcggt caaagggac gctcaagaa 
      gcacatcag gacgcatac gggggaaag accgtatgt 
      gtgcagtgt gtgcggaca ggcgttcgc ccagagcgg 
      gaccctggc cacgcacat gaaggtgca caggcccaa 
      gccgtaatt ttttgattt tgtgatttt tatttgatc 
      tcgtgaatc taaatatgc g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY