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Gene information
General information
TC014225 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3457685..3469114
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014225
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02262

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 head musculature not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: All kind of head defects or missing head parts are possible. From just shortened antennae (as shown) to several completely missing head appendages.
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014225 RA
      MAESGIDLG YDLSALLSD DFDIESAID FDDLLGTPK 
      NQEFYELTS KTMPVNESP PNLKTATPL SRTQLKLQL 
      MRDQALMEQ ERQAQERAR QAQAMQQRP PASAMKVPL 
      HSLAVEVPP QVLQVQTKL ANPTRYHVI QKQKSQVRQ 
      YLSESFQAP GNAHFLNNL QNPVPQTHS APGIAGAPV 
      PSAPLMPQQ SLAHSHPFQ VSCPSPEAV AMSPALSSG 
      ATSTSEAED LIDDLLSLE SSSLASDSF KTSDNSLPG 
      NDINIKNEP FVLSDAELH ALAKDRQKK DNHNMIERR 
      RRFNINDRI KELGTLLPK NNDPYYEIV RDVRPNKGT 
      ILKSSVEYI KCLKNEVQR LKQSEIRQK QIEHINRRL 
      QLRVQELER QMKSHGLPL SEFNFQGYN SPYNSYQKS 
      QNQPPPTAL LPPIMLSDP NKKGADVAF QMPDVINDA 
      ALSMSTMEE LMDDDEPVN GDPMLSSPN DLSGEQLLT 
      SPALCEPRL PTPTPTSHI EHVDGDTLD IDMIA
    • >TC014225 RB
      MAESGIDLG YDLSALLSD DFDIESAID FDDLLGTPK 
      NQEFYELTS KTMPVNESP PNLKTATPL SRTQLKLQL 
      MRDQALMEQ ERQAQERAR QAQAMQQRP PASAMKVPL 
      HSLAVEVPP QVLQVQTKL ANPTRYHVI QKQKSQVRQ 
      YLSESFQAP GNAHFLNNL QNPVPQTHS APGIAGAPV 
      PSAPLMPQQ SLAHSHPFQ VSCPSPEAV AMSPALSSG 
      ATSTSEPPP KMPVYNIIT RRCKAEDLI DDLLSLESS 
      SLASDSFKT SDNSLPGND INIKNEPFV LSDAELHAL 
      AKDRQKKDN HNMIERRRR FNINDRIKE LGTLLPKNN 
      DPYYEIVRD VRPNKGTIL KSSVEYIKC LKNEVQRLK 
      QSEIRQKQI EHINRRLQL RVQELERQM KSHGLPLSE 
      FNFQGYNSP YNSYQKSQN QPPPTALLP PIMLSDPNK 
      KGADVAFQM PDVINDAAL SMSTMEELM DDDEPVNGD 
      PMLSSPNDL SGEQLLTSP ALCEPRLPT PTPTSHIEH 
      VDGDTLDID MIA
    CDS sequence
    • >TC014225 RA
      atggccgaa tctggtatt gatttaggt tacgatctg 
      tcggctctc ttgtccgac gatttcgat atagaaagt 
      gcgattgat ttcgatgat ctcttgggt actccgaaa 
      aatcaagag ttttacgaa ctaacttct aaaacaatg 
      cctgtaaac gaaagtccg ccgaaccta aaaaccgcc 
      accccattg tcccgaacg cagttgaag ctgcagctg 
      atgcgcgac caggcgctg atggagcag gaaagacag 
      gcccaagaa cgagcaaga caagctcaa gccatgcaa 
      caaagaccg ccagcctca gccatgaaa gtgccattg 
      cacagttta gcggtagag gttcctccc caagtttta 
      caagttcaa acaaaacta gcaaatcct actaggtac 
      catgtgata cagaaacag aaaagtcaa gtgaggcag 
      tatctcagc gaatcattc caagctccg ggcaatgca 
      cacttcttg aacaatttg caaaatccg gtgccgcaa 
      acgcacagc gcgcccgga attgccggt gctccggta 
      ccttccgcc cccctaatg ccgcagcag tcccttgct 
      cactcgcac ccctttcaa gtttcgtgc cctagtcct 
      gaggctgtg gccatgtcc ccagcgctc agctcaggc 
      gccaccagc acctctgag gctgaagat cttattgac 
      gatctgctc tcgttggaa tcaagttcc ctcgcttct 
      gatagtttc aaaacttcg gacaattcg ttaccagga 
      aacgatata aatatcaaa aatgaacct tttgttctt 
      agcgatgct gaactccat gctcttgca aaagaccga 
      caaaagaaa gataaccat aatatgatt gaacgtagg 
      cgaaggttc aacataaat gatcgaata aaagaatta 
      ggtactctc ttgccgaag aataatgat ccttattac 
      gaaatagtg cgagacgtt cgccccaac aaaggaacg 
      atattaaaa tcatcagta gaatatata aaatgtctc 
      aaaaacgaa gtacaaagg ctaaagcaa tccgaaatt 
      agacaaaaa caaatcgaa cacatcaac agaaggctg 
      cagctccga gtccaggaa ctcgaacgc cagatgaaa 
      tctcacggc ttgccctta tcagagttc aacttccaa 
      ggctacaac tcgccctac aactcctac cagaagagc 
      cagaaccag ccgcccccg accgccctc ctgcccccc 
      atcatgctg tcagacccc aacaagaag ggtgcggat 
      gtggccttc cagatgccg gacgtgatc aacgacgcc 
      gccctgagc atgtccacc atggaggag ctgatggac 
      gacgacgag ccggtgaac ggcgacccc atgctgtcc 
      tcccccaac gacctctcg ggggagcag ctgctgacc 
      tcccccgca ctctgcgag ccccgactg cccaccccc 
      acgcccacc agccacata gaacacgtc gacggcgac 
      accctcgat attgacatg attgcatga
    • >TC014225 RB
      atggccgaa tctggtatt gatttaggt tacgatctg 
      tcggctctc ttgtccgac gatttcgat atagaaagt 
      gcgattgat ttcgatgat ctcttgggt actccgaaa 
      aatcaagag ttttacgaa ctaacttct aaaacaatg 
      cctgtaaac gaaagtccg ccgaaccta aaaaccgcc 
      accccattg tcccgaacg cagttgaag ctgcagctg 
      atgcgcgac caggcgctg atggagcag gaaagacag 
      gcccaagaa cgagcaaga caagctcaa gccatgcaa 
      caaagaccg ccagcctca gccatgaaa gtgccattg 
      cacagttta gcggtagag gttcctccc caagtttta 
      caagttcaa acaaaacta gcaaatcct actaggtac 
      catgtgata cagaaacag aaaagtcaa gtgaggcag 
      tatctcagc gaatcattc caagctccg ggcaatgca 
      cacttcttg aacaatttg caaaatccg gtgccgcaa 
      acgcacagc gcgcccgga attgccggt gctccggta 
      ccttccgcc cccctaatg ccgcagcag tcccttgct 
      cactcgcac ccctttcaa gtttcgtgc cctagtcct 
      gaggctgtg gccatgtcc ccagcgctc agctcaggc 
      gccaccagc acctctgag ccgccgccg aaaatgccg 
      gtgtacaat atcatcacc aggagatgt aaggctgaa 
      gatcttatt gacgatctg ctctcgttg gaatcaagt 
      tccctcgct tctgatagt ttcaaaact tcggacaat 
      tcgttacca ggaaacgat ataaatatc aaaaatgaa 
      ccttttgtt cttagcgat gctgaactc catgctctt 
      gcaaaagac cgacaaaag aaagataac cataatatg 
      attgaacgt aggcgaagg ttcaacata aatgatcga 
      ataaaagaa ttaggtact ctcttgccg aagaataat 
      gatccttat tacgaaata gtgcgagac gttcgcccc 
      aacaaagga acgatatta aaatcatca gtagaatat 
      ataaaatgt ctcaaaaac gaagtacaa aggctaaag 
      caatccgaa attagacaa aaacaaatc gaacacatc 
      aacagaagg ctgcagctc cgagtccag gaactcgaa 
      cgccagatg aaatctcac ggcttgccc ttatcagag 
      ttcaacttc caaggctac aactcgccc tacaactcc 
      taccagaag agccagaac cagccgccc ccgaccgcc 
      ctcctgccc cccatcatg ctgtcagac cccaacaag 
      aagggtgcg gatgtggcc ttccagatg ccggacgtg 
      atcaacgac gccgccctg agcatgtcc accatggag 
      gagctgatg gacgacgac gagccggtg aacggcgac 
      cccatgctg tcctccccc aacgacctc tcgggggag 
      cagctgctg acctccccc gcactctgc gagccccga 
      ctgcccacc cccacgccc accagccac atagaacac 
      gtcgacggc gacaccctc gatattgac atgattgca 
      tga
    mRNA sequence
    • >TC014225 RA
      ttttaataa acttcggct gctgtttgg ctgtcggtc 
      gtccaaaca gtccgtcgg cggattaga agagatatg 
      ccaccgacc aacaccacc taattatcc taaacaaat 
      ctgtgcatt gagctgtga aaaatggcc gaatctggt 
      attgattta ggttacgat ctgtcggct ctcttgtcc 
      gacgatttc gatatagaa agtgcgatt gatttcgat 
      gatctcttg ggtactccg aaaaatcaa gagttttac 
      gaactaact tctaaaaca atgcctgta aacgaaagt 
      ccgccgaac ctaaaaacc gccacccca ttgtcccga 
      acgcagttg aagctgcag ctgatgcgc gaccaggcg 
      ctgatggag caggaaaga caggcccaa gaacgagca 
      agacaagct caagccatg caacaaaga ccgccagcc 
      tcagccatg aaagtgcca ttgcacagt ttagcggta 
      gaggttcct ccccaagtt ttacaagtt caaacaaaa 
      ctagcaaat cctactagg taccatgtg atacagaaa 
      cagaaaagt caagtgagg cagtatctc agcgaatca 
      ttccaagct ccgggcaat gcacacttc ttgaacaat 
      ttgcaaaat ccggtgccg caaacgcac agcgcgccc 
      ggaattgcc ggtgctccg gtaccttcc gccccccta 
      atgccgcag cagtccctt gctcactcg caccccttt 
      caagtttcg tgccctagt cctgaggct gtggccatg 
      tccccagcg ctcagctca ggcgccacc agcacctct 
      gaggctgaa gatcttatt gacgatctg ctctcgttg 
      gaatcaagt tccctcgct tctgatagt ttcaaaact 
      tcggacaat tcgttacca ggaaacgat ataaatatc 
      aaaaatgaa ccttttgtt cttagcgat gctgaactc 
      catgctctt gcaaaagac cgacaaaag aaagataac 
      cataatatg attgaacgt aggcgaagg ttcaacata 
      aatgatcga ataaaagaa ttaggtact ctcttgccg 
      aagaataat gatccttat tacgaaata gtgcgagac 
      gttcgcccc aacaaagga acgatatta aaatcatca 
      gtagaatat ataaaatgt ctcaaaaac gaagtacaa 
      aggctaaag caatccgaa attagacaa aaacaaatc 
      gaacacatc aacagaagg ctgcagctc cgagtccag 
      gaactcgaa cgccagatg aaatctcac ggcttgccc 
      ttatcagag ttcaacttc caaggctac aactcgccc 
      tacaactcc taccagaag agccagaac cagccgccc 
      ccgaccgcc ctcctgccc cccatcatg ctgtcagac 
      cccaacaag aagggtgcg gatgtggcc ttccagatg 
      ccggacgtg atcaacgac gccgccctg agcatgtcc 
      accatggag gagctgatg gacgacgac gagccggtg 
      aacggcgac cccatgctg tcctccccc aacgacctc 
      tcgggggag cagctgctg acctccccc gcactctgc 
      gagccccga ctgcccacc cccacgccc accagccac 
      atagaacac gtcgacggc gacaccctc gatattgac 
      atgattgca tgatattag cgtccgtgc ggactgggt 
      tcgacgagt cccatggac agtgactgc aatattacc 
      attaccatt attaccggt ttacttaaa tcttattac 
      agcatttag agaattcga ctataacat cagttaggt 
      acttttgtt atgtagaga tattgtatt gcgcctcgc 
      gtgcctcgt agctcaaat tttctagat tgtccgaga 
      ttgagatac ctactatgt taaggaata agccaaata 
      tgtgtaaat aaaagtatt gtgatgttc tgggtaggg 
      tgttaccat ttttgttgt tgttaataa caaaattat 
      agattttaa cattgtttt aaaatgatt attaaaaat 
      tatcgacga tt
    • >TC014225 RB
      ttttaataa acttcggct gctgtttgg ctgtcggtc 
      gtccaaaca gtccgtcgg cggattaga agagatatg 
      ccaccgacc aacaccacc taattatcc taaacaaat 
      ctgtgcatt gagctgtga aaaatggcc gaatctggt 
      attgattta ggttacgat ctgtcggct ctcttgtcc 
      gacgatttc gatatagaa agtgcgatt gatttcgat 
      gatctcttg ggtactccg aaaaatcaa gagttttac 
      gaactaact tctaaaaca atgcctgta aacgaaagt 
      ccgccgaac ctaaaaacc gccacccca ttgtcccga 
      acgcagttg aagctgcag ctgatgcgc gaccaggcg 
      ctgatggag caggaaaga caggcccaa gaacgagca 
      agacaagct caagccatg caacaaaga ccgccagcc 
      tcagccatg aaagtgcca ttgcacagt ttagcggta 
      gaggttcct ccccaagtt ttacaagtt caaacaaaa 
      ctagcaaat cctactagg taccatgtg atacagaaa 
      cagaaaagt caagtgagg cagtatctc agcgaatca 
      ttccaagct ccgggcaat gcacacttc ttgaacaat 
      ttgcaaaat ccggtgccg caaacgcac agcgcgccc 
      ggaattgcc ggtgctccg gtaccttcc gccccccta 
      atgccgcag cagtccctt gctcactcg caccccttt 
      caagtttcg tgccctagt cctgaggct gtggccatg 
      tccccagcg ctcagctca ggcgccacc agcacctct 
      gagccgccg ccgaaaatg ccggtgtac aatatcatc 
      accaggaga tgtaaggct gaagatctt attgacgat 
      ctgctctcg ttggaatca agttccctc gcttctgat 
      agtttcaaa acttcggac aattcgtta ccaggaaac 
      gatataaat atcaaaaat gaacctttt gttcttagc 
      gatgctgaa ctccatgct cttgcaaaa gaccgacaa 
      aagaaagat aaccataat atgattgaa cgtaggcga 
      aggttcaac ataaatgat cgaataaaa gaattaggt 
      actctcttg ccgaagaat aatgatcct tattacgaa 
      atagtgcga gacgttcgc cccaacaaa ggaacgata 
      ttaaaatca tcagtagaa tatataaaa tgtctcaaa 
      aacgaagta caaaggcta aagcaatcc gaaattaga 
      caaaaacaa atcgaacac atcaacaga aggctgcag 
      ctccgagtc caggaactc gaacgccag atgaaatct 
      cacggcttg cccttatca gagttcaac ttccaaggc 
      tacaactcg ccctacaac tcctaccag aagagccag 
      aaccagccg cccccgacc gccctcctg ccccccatc 
      atgctgtca gaccccaac aagaagggt gcggatgtg 
      gccttccag atgccggac gtgatcaac gacgccgcc 
      ctgagcatg tccaccatg gaggagctg atggacgac 
      gacgagccg gtgaacggc gaccccatg ctgtcctcc 
      cccaacgac ctctcgggg gagcagctg ctgacctcc 
      cccgcactc tgcgagccc cgactgccc acccccacg 
      cccaccagc cacatagaa cacgtcgac ggcgacacc 
      ctcgatatt gacatgatt gcatgatat tagcgtccg 
      tgcggactg ggttcgacg agtcccatg gacagtgac 
      tgcaatatt accattacc attattacc ggtttactt 
      aaatcttat tacagcatt tagagaatt cgactataa 
      catcagtta ggtactttt gttatgtag agatattgt 
      attgcgcct cgcgtgcct cgtagctca aattttcta 
      gattgtccg agattgaga tacctacta tgttaagga 
      ataagccaa atatgtgta aataaaagt attgtgatg 
      ttctgggta gggtgttac catttttgt tgttgttaa 
      taacaaaat tatagattt taacattgt tttaaaatg 
      attattaaa aattatcga cgatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY