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Gene information
General information
TC014220 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 3175070..3179299
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014220
    iBeetle screen iB_02261
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 3

    Comment: The "held egg" ovary also showed the accumulation of potentially mature eggs in some of the ovarioles
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50
    about 50
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014220 RA
      MSSDNKQVN IDVQEPLLE HSGKKPATQ IENLPKNDA 
      ETNNKHKSS DMERKRTKS VAIIAEKDE KKMDKWKLV 
      PPDGGWGWL VLFGSTLVN VLVPGTVKS FGVLKVEFE 
      DALNATPDD AAWVAAICY FLYSSLGPL SSILSVKYS 
      YRTVTLIGG TFAAAGMIL SFFANSVAY LCISYGVLV 
      GTGAGLSFP PTVYIVTSY FVRLRGLAN GLCISGSAL 
      GSIILPPVL DLLLGIYGY RGAVLLMGG VTLNVWVAA 
      LFYDPVEKH MKKVPIEPK DSESEEASP LKPKFVIST 
      EDSMASLQH IPHNDSFLE NLDISSNNF NRSVSSAAM 
      QNFKAPTSR ERKISMPTG RNELMKAGT GSRTNMNSS 
      SALHAVPES GNGAMQSQG RLTSSRRIR VPKRSPSTS 
      SFQYVSTPY HGSTLTLQP ETFASSFSL KSVKKTDGK 
      EPPVKKNKF FDISLLKDP LYLVILISN ATNAVSYTN 
      FIILLPSFA KELGYNMTM GALLLSIVS ALDLVGRIG 
      GSALSDLHL CPKAAYFIG GLLVSGISL GIMPFFTGY 
      GMISFFCAV FGLASGIYV GVTAVIMAD MLGEERLQS 
      TYGISLFVN GILQLVGPP LCGIWFEQT KSFVSLFCI 
      LGVILVVGA SIWVFVPFI RKPRSEDSD DDDEDSS
    CDS sequence
    • >TC014220 RA
      atgtccagc gacaataag caagtgaac attgatgtg 
      caagaacct ttgcttgaa cactccggg aaaaaaccg 
      gcaacgcaa atagaaaat ttgccgaaa aatgacgcc 
      gaaaccaac aataaacac aagtcgagc gatatggaa 
      agaaaacgc acaaaaagt gtggcgata attgctgaa 
      aaggatgag aaaaaaatg gacaagtgg aagctggta 
      ccacccgac ggtggatgg ggttggcta gtacttttc 
      ggctcaact ctagtcaac gtcttggtg ccaggtaca 
      gtaaagtct ttcggcgtc ttaaaagtc gaattcgag 
      gacgccctc aatgctacg cccgacgat gcagcctgg 
      gtggcagcc atctgctac ttcctctac agctctcta 
      ggccccttg tccagcatc ctctcagtc aagtactcc 
      taccgaacg gtcacccta atcggaggc accttcgca 
      gccgccggc atgatcctc agcttcttt gcgaactcg 
      gtggcctat ctctgcatc agttacggc gtcttggtt 
      gggacgggt gccggtctt tcgttcccc ccgacagtc 
      tatatcgtg actagttat tttgtgaga cttcggggc 
      ctagcgaac ggcctgtgc atatcaggg tcggctctc 
      ggttcgatt attctcccc ccagtgctt gacctcctg 
      ttggggatc tacgggtac aggggcgcc gttttgctc 
      atgggggga gtgacgttg aacgtgtgg gtggccgca 
      ctgttctac gaccccgtg gaaaagcac atgaagaag 
      gtccccatc gagccgaaa gactccgag tcggaggaa 
      gcaagtcct ttgaagcca aaattcgtg atcagcacc 
      gaagacagc atggcgtct ttgcagcac atcccacac 
      aacgactcg tttttggaa aacttggac atctcatcg 
      aacaacttc aacaggagt gtttcaagc gccgccatg 
      cagaatttc aaggcgccg accagccgc gagcggaaa 
      atcagtatg cccactggt cgcaacgag ctaatgaaa 
      gcgggcacg ggttctcgt accaacatg aatagttct 
      tcggccttg cacgcggtg ccggaaagc ggcaatggg 
      gccatgcag tcgcaaggc cgcttgacg agctcgagg 
      cgaatccgg gttccgaag cggagcccc tcgactagt 
      tccttccag tacgttagt actccctac catgggagt 
      accctgacg ctccaaccg gagacattc gccagttcg 
      ttcagtctc aaatcggtt aagaaaacc gacgggaag 
      gaaccccca gttaagaag aacaaattt ttcgatatt 
      tcgctcctg aaagacccg ctctatttg gtcatcctg 
      atatcaaac gccacaaat gccgtcagc tacaccaac 
      ttcattatc ctcctcccg tcgttcgcc aaagagttg 
      gggtacaac atgacaatg ggggcgctg ctcctttca 
      attgtctcg gctctggac cttgtgggt aggatcgga 
      gggtcggct ttatccgac ttgcatttg tgtccaaaa 
      gccgcgtat ttcatcggt gggcttttg gtgtccggc 
      atttcgctc ggcatcatg ccatttttc accggctat 
      ggcatgatc agtttcttt tgtgccgtc tttggcctg 
      gcgtctggt atttacgtg ggggtcacc gcagtgata 
      atggccgat atgttaggc gaggaacgg ttgcaatca 
      acttacggc atatctctg tttgttaat ggcatttta 
      caattagtg ggccctccc ctttgtggc atctggttc 
      gagcaaacc aagtccttc gtatcattg ttttgtatc 
      ttaggtgtc attcttgtc gttggtgcc tcgatttgg 
      gtcttcgtc ccctttata cgaaagcct cggtccgag 
      gacagtgat gatgatgat gaagattca tcatag
    mRNA sequence
    • >TC014220 RA
      gttcaaggg aacctaaac taaaacatt cacctctga 
      acttttttc tccattatt tattgctgc gggcgtccg 
      aaaatgacg taataaaat agtaatgcg gcagattga 
      taatttttc atgccagtt gattaaaaa cgtaactgg 
      aacatgttg gtttgttag ataagtgat tgaattgaa 
      gggtacaag ggctaaaaa tcgcggtca tagaaagga 
      aactaaaag aggtgtatt agatagaaa tgtgtcagt 
      tgtttagtg ttttatgag agttggttg ataaacaga 
      ataattact ggcgatgta tgcgatgtt aattcgtaa 
      ttaaagcat ttggtgcga gcgaaaatc gtgcgtcgg 
      tgtggtttg tccgataag tatgataaa tcaaatctt 
      gtgattgaa aacatcgcg ataactagt gtatgttga 
      atgctctca gaatcgcgc aaggtaata ttaatgaga 
      gcattgtgt aacataaaa taaataatc caattgtgt 
      ccttcattc catgtgtgc ataatcgga attcagtta 
      tctccgcaa aaactgaat ggccaccag tctgtgttc 
      ggattgcct caaattttt ttcttttca attgaattc 
      cagtcgtgt taatgataa gtttttatc gggtaaatc 
      atcagctgt tcgcatacc gttttagac tttcaggca 
      cgtgttcgt gctaagcga tcagtgttg tttcgtgtt 
      ttctcttaa acatataac aaaaaatgt ccagcgaca 
      ataagcaag tgaacattg atgtgcaag aacctttgc 
      ttgaacact ccgggaaaa aaccggcaa cgcaaatag 
      aaaatttgc cgaaaaatg acgccgaaa ccaacaata 
      aacacaagt cgagcgata tggaaagaa aacgcacaa 
      aaagtgtgg cgataattg ctgaaaagg atgagaaaa 
      aaatggaca agtggaagc tggtaccac ccgacggtg 
      gatggggtt ggctagtac ttttcggct caactctag 
      tcaacgtct tggtgccag gtacagtaa agtctttcg 
      gcgtcttaa aagtcgaat tcgaggacg ccctcaatg 
      ctacgcccg acgatgcag cctgggtgg cagccatct 
      gctacttcc tctacagct ctctaggcc ccttgtcca 
      gcatcctct cagtcaagt actcctacc gaacggtca 
      ccctaatcg gaggcacct tcgcagccg ccggcatga 
      tcctcagct tctttgcga actcggtgg cctatctct 
      gcatcagtt acggcgtct tggttggga cgggtgccg 
      gtctttcgt tccccccga cagtctata tcgtgacta 
      gttattttg tgagacttc ggggcctag cgaacggcc 
      tgtgcatat cagggtcgg ctctcggtt cgattattc 
      tccccccag tgcttgacc tcctgttgg ggatctacg 
      ggtacaggg gcgccgttt tgctcatgg ggggagtga 
      cgttgaacg tgtgggtgg ccgcactgt tctacgacc 
      ccgtggaaa agcacatga agaaggtcc ccatcgagc 
      cgaaagact ccgagtcgg aggaagcaa gtcctttga 
      agccaaaat tcgtgatca gcaccgaag acagcatgg 
      cgtctttgc agcacatcc cacacaacg actcgtttt 
      tggaaaact tggacatct catcgaaca acttcaaca 
      ggagtgttt caagcgccg ccatgcaga atttcaagg 
      cgccgacca gccgcgagc ggaaaatca gtatgccca 
      ctggtcgca acgagctaa tgaaagcgg gcacgggtt 
      ctcgtacca acatgaata gttcttcgg ccttgcacg 
      cggtgccgg aaagcggca atggggcca tgcagtcgc 
      aaggccgct tgacgagct cgaggcgaa tccgggttc 
      cgaagcgga gcccctcga ctagttcct tccagtacg 
      ttagtactc cctaccatg ggagtaccc tgacgctcc 
      aaccggaga cattcgcca gttcgttca gtctcaaat 
      cggttaaga aaaccgacg ggaaggaac ccccagtta 
      agaagaaca aatttttcg atatttcgc tcctgaaag 
      acccgctct atttggtca tcctgatat caaacgcca 
      caaatgccg tcagctaca ccaacttca ttatcctcc 
      tcccgtcgt tcgccaaag agttggggt acaacatga 
      caatggggg cgctgctcc tttcaattg tctcggctc 
      tggaccttg tgggtagga tcggagggt cggctttat 
      ccgacttgc atttgtgtc caaaagccg cgtatttca 
      tcggtgggc ttttggtgt ccggcattt cgctcggca 
      tcatgccat ttttcaccg gctatggca tgatcagtt 
      tcttttgtg ccgtctttg gcctggcgt ctggtattt 
      acgtggggg tcaccgcag tgataatgg ccgatatgt 
      taggcgagg aacggttgc aatcaactt acggcatat 
      ctctgtttg ttaatggca ttttacaat tagtgggcc 
      ctccccttt gtggcatct ggttcgagc aaaccaagt 
      ccttcgtat cattgtttt gtatcttag gtgtcattc 
      ttgtcgttg gtgcctcga tttgggtct tcgtcccct 
      ttatacgaa agcctcggt ccgaggaca gtgatgatg 
      atgatgaag attcatcat agtttcaat taattgatt 
      tctttatgt ttgtgtgtt tttttagtg cgttattgt 
      cgttccctc tttttgcta ggaatgata gttagcaag 
      tatttagtt tatttttat taggtacaa tacgttatt 
      acatcggtc aggacacat tccagctca atcttttac 
      tgactcttg atctgtgta gaggtcttc ctgacgata 
      tttttgaaa acaaagaaa atttcaaac tgaacaact 
      atccatatc aaagaaggt gctgtcaag tcgagtatt 
      tgtattttt atattgtta tttatacga agtgtagga 
      tttcaactt acttacatt gtgatataa acttattaa 
      acgtcctct gtaggttac cgaaaataa aattacaaa 
      ctaaca