Status
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Gene information
General information
TC014207 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3195038..3197891
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014207
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02260

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: The head often appears smaller, in some cases also complete parts (appendages etc.) or almost the whole head are missing.
    head appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014207 RA
      MANSKTDVQ TEVENVRVF VRVRPLTKK EEAEGHLNV 
      LLIDPKENL IALNKDGAN PKPFKFDQV FGEDSTQLS 
      LYRVIAVPI VEKALQGYN GTIFAYGQT GTGKTYTMS 
      GNYAKPELK GIIPNTFSH IFSQISRAS GETSFVVTV 
      TYLEIYNEE VRDLLSTDP NKKLAIRER PDVGVYVKD 
      LMGFTVDSI ESITELLNR GNKNRVTRS TLMNDVSSR 
      SHAIFTITI ESKNRSSNK TTVGKLNLV DLAGSERAS 
      RTQATGERL REASNINLS LSVLGNVIS ALVDGKSSH 
      IPYRNSKLT RLLQDSLGG NSKTAMIAM VSPADIDYE 
      ESICTLRYA ARVKHIQNH ARINVEQRG LIEGFEHEI 
      AELQEKIHL LSLQEQKQP KKKKERASA HKVEELKKW 
      EEELQKTEK EKSDLMQKI SLIQKRILV GGENLFEKA 
      QHQAFLIES TGAEIENLD KNHQQLEET LSKKGQERI 
      DVEEKYSSL QEEDAGTGK KIKKVQAYL NEVKEEYAD 
      KEHEYQREM EALLDNNRL LVRETQLAC LMIDSYIPK 
      EYLKKIEAN LIWNSETQE YQMRGVAYT GNNMRKRQS 
      AHSECFTPV KFEMKPVYH KYPEKKRER KMVYEKRSF 
      SAVPKAR
    CDS sequence
    • >TC014207 RA
      atggcaaat tcgaagaca gacgtgcag actgaggtg 
      gaaaacgtt cgtgttttt gtccgagtg cgtccttta 
      accaagaag gaagaagct gagggccac ctaaatgtt 
      cttttaatc gaccctaag gagaattta attgctttg 
      aataaagac ggggcgaac cccaaaccg tttaagttc 
      gatcaggtg tttggggag gactcgacg cagttgtcg 
      ctgtacaga gtgatagct gtgccaata gtcgagaag 
      gccctacag gggtacaat ggcacgatt tttgcgtac 
      ggccagacg ggcaccggg aagacgtac accatgtca 
      gggaattac gccaagccg gaactcaaa ggcatcatc 
      cccaacaca ttctcgcac attttcagc cagatttcg 
      cgggccagc ggcgagacg tcgttcgtt gtcaccgtc 
      acctacctc gagatctac aacgaagaa gtcagggat 
      ttgctgtcc acagacccg aataagaaa ttggcgatt 
      cgggagagg ccagacgtg ggcgtgtat gtcaaggat 
      ctcatggga ttcaccgtt gactcgatt gagtcaatt 
      accgaactc ctcaacaga ggaaacaaa aaccgcgtc 
      actcggtcc actctcatg aacgacgtg agctcgaga 
      tcgcacgcg atttttacc ataaccatc gagtcgaaa 
      aacagaagc agcaataaa accactgtt ggcaaactc 
      aatttggtt gacttggct ggctccgag cgggcaagc 
      aggacccaa gccacaggc gagcggctg agggaggcc 
      agcaatatt aatctgtcg ctctcggtg ctcggaaat 
      gtcatttcg gcactcgtt gacggcaaa agcagccac 
      attccgtac agaaactcc aaactgacg cggttactc 
      caagactcg ctgggcgga aactccaaa acagcaatg 
      atcgcgatg gtgagtccg gcggacatt gactacgaa 
      gaaagcatt tgtacgtta agatacgcc gctcgagtc 
      aaacacatc caaaaccat gcgcgaatt aacgtcgaa 
      cagcggggt cttattgag ggattcgag cacgaaatc 
      gccgaattg caggaaaaa atccactta ttgtcactc 
      caggagcaa aaacagccc aagaaaaag aaagagcgg 
      gcgtcggcc cacaaagtt gaggaactg aagaagtgg 
      gaagaggaa ttgcaaaaa actgagaag gaaaagagc 
      gatttgatg cagaaaatc tcgctgatc cagaagcgg 
      atcctcgtc ggcggtgag aacctgttc gagaaggcg 
      cagcaccag gccttcctg atcgagtcg acgggggcg 
      gagatcgaa aacctggac aagaaccac caacagctg 
      gaggagacg ctgagcaag aaggggcag gagcgcatc 
      gacgtggag gagaagtac tcgagtctc caggaggag 
      gacgccggg acgggcaaa aagatcaaa aaagtccag 
      gcgtatctg aacgaagtc aaggaggag tacgcggac 
      aaggagcac gagtaccag cgcgaaatg gaggcgctg 
      ctggacaac aaccggctc ctggtccgg gaaacccag 
      ctggcgtgc ctgatgatc gacagctac atcccgaag 
      gaatacttg aagaaaatc gaggccaat ttgatctgg 
      aactcggag acgcaggaa taccagatg cgcggggtg 
      gcctacacg gggaacaac atgcggaag cggcagtcg 
      gcccactcg gagtgcttc acgccggtg aagttcgag 
      atgaagccg gtgtaccac aagtacccg gagaagaag 
      cgcgagcgc aagatggtg tacgagaag cggtcgttc 
      tccgcggtg cccaaagcc cgctaa
    mRNA sequence
    • >TC014207 RA
      attatttaa taatgccct tatcactat cccaacacc 
      ggggattta attagctcg ttcgagtgg ctgtttact 
      tttcggctg ccccttgta gctcgcagc ctaagaccg 
      attcaaact ctgcgcgct tgtagctgt cagactgac 
      agcttaccc taacctaaa tttccgcaa tattgatta 
      aatttcccg taaaaaatt ttcaaaaat ggcaaattc 
      gaagacaga cgtgcagac tgaggtgga aaacgttcg 
      tgtttttgt ccgagtgcg tcctttaac caagaagga 
      agaagctga gggccacct aaatgttct tttaatcga 
      ccctaagga gaatttaat tgctttgaa taaagacgg 
      ggcgaaccc caaaccgtt taagttcga tcaggtgtt 
      tggggagga ctcgacgca gttgtcgct gtacagagt 
      gatagctgt gccaatagt cgagaaggc cctacaggg 
      gtacaatgg cacgatttt tgcgtacgg ccagacggg 
      caccgggaa gacgtacac catgtcagg gaattacgc 
      caagccgga actcaaagg catcatccc caacacatt 
      ctcgcacat tttcagcca gatttcgcg ggccagcgg 
      cgagacgtc gttcgttgt caccgtcac ctacctcga 
      gatctacaa cgaagaagt cagggattt gctgtccac 
      agacccgaa taagaaatt ggcgattcg ggagaggcc 
      agacgtggg cgtgtatgt caaggatct catgggatt 
      caccgttga ctcgattga gtcaattac cgaactcct 
      caacagagg aaacaaaaa ccgcgtcac tcggtccac 
      tctcatgaa cgacgtgag ctcgagatc gcacgcgat 
      ttttaccat aaccatcga gtcgaaaaa cagaagcag 
      caataaaac cactgttgg caaactcaa tttggttga 
      cttggctgg ctccgagcg ggcaagcag gacccaagc 
      cacaggcga gcggctgag ggaggccag caatattaa 
      tctgtcgct ctcggtgct cggaaatgt catttcggc 
      actcgttga cggcaaaag cagccacat tccgtacag 
      aaactccaa actgacgcg gttactcca agactcgct 
      gggcggaaa ctccaaaac agcaatgat cgcgatggt 
      gagtccggc ggacattga ctacgaaga aagcatttg 
      tacgttaag atacgccgc tcgagtcaa acacatcca 
      aaaccatgc gcgaattaa cgtcgaaca gcggggtct 
      tattgaggg attcgagca cgaaatcgc cgaattgca 
      ggaaaaaat ccacttatt gtcactcca ggagcaaaa 
      acagcccaa gaaaaagaa agagcgggc gtcggccca 
      caaagttga ggaactgaa gaagtggga agaggaatt 
      gcaaaaaac tgagaagga aaagagcga tttgatgca 
      gaaaatctc gctgatcca gaagcggat cctcgtcgg 
      cggtgagaa cctgttcga gaaggcgca gcaccaggc 
      cttcctgat cgagtcgac gggggcgga gatcgaaaa 
      cctggacaa gaaccacca acagctgga ggagacgct 
      gagcaagaa ggggcagga gcgcatcga cgtggagga 
      gaagtactc gagtctcca ggaggagga cgccgggac 
      gggcaaaaa gatcaaaaa agtccaggc gtatctgaa 
      cgaagtcaa ggaggagta cgcggacaa ggagcacga 
      gtaccagcg cgaaatgga ggcgctgct ggacaacaa 
      ccggctcct ggtccggga aacccagct ggcgtgcct 
      gatgatcga cagctacat cccgaagga atacttgaa 
      gaaaatcga ggccaattt gatctggaa ctcggagac 
      gcaggaata ccagatgcg cggggtggc ctacacggg 
      gaacaacat gcggaagcg gcagtcggc ccactcgga 
      gtgcttcac gccggtgaa gttcgagat gaagccggt 
      gtaccacaa gtacccgga gaagaagcg cgagcgcaa 
      gatggtgta cgagaagcg gtcgttctc cgcggtgcc 
      caaagcccg ctaactgtc gccggatta tttgtgttg 
      tgttaattt gtgatatta attaggccc gacttttta 
      tttatttgt gattatgtt gtacttaat aaatctatt 
      tattgccc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY