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Gene information
General information
TC014203 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 2870684..2879128
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014203
    iBeetle screen iB_02259
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014203 RA
      MVAGVKWKD AFCVILVTV SFKSWDNFV YGQICNHVH 
      PKHDEVSHT HIEPAHVMK KRSPDQSLR ILLYYDSSV 
      YRLDRERFD LINETILPQ AVTFWEKAL FVRRVESVI 
      RLTRKCNNT QVFVKNSLT HCIDTCKEK TMCGEVVVP 
      EEHLDACRT CNATGQDCG IAKGSKAGE GIENFDFVF 
      YVSAMQTER CNKSLTVAY AAHCQQEAA LDRPIAGHA 
      NLCPNSIST KRQEVEILL STVKHEILH ALGFSVSLY 
      AFYRDSKGE PLTERRLDT GKPPLNETL QTYQWSDKV 
      IRSFVRKNW LVKSGVMRR EVNMIVTRN VVREVRDYF 
      KCEELEGAE LEDQGEEGT ALTHWEKRV FENEAMTGT 
      HTQNPIISR ITLALMEDT GWYLANYSM AEPMSWGKG 
      LGCDFVMKS CKEWITIKS SRGASIHPF CNKVKRDPL 
      QTECTDDRT SVALCNLVA HETKLPQLY QNFDYIKYV 
      EPGTEEYYG GSVALADYC PYIQEFTWR SKNVIVRGS 
      HCQYAENNP KPDKNFALE KYGENSRCF EHTNQMWEE 
      RSCGQMRQW QHWGSGCYG YRCQHGRLH ILVDNYSYE 
      CFHPNQEIS IRIINNGWL HKGAIVCPP CRELCAREF 
      AQRNESCKG NDEAPPATA YPRDELKCS STIPRLSGS 
      LLLLILFFS TCAREHLV
    CDS sequence
    • >TC014203 RA
      atggtggct ggagttaaa tggaaggac gctttttgt 
      gttattcta gtgacagtt tcattcaaa tcttgggat 
      aatttcgtg tatggacaa atttgtaat catgtgcat 
      cccaaacac gacgaggtg tctcacacg cacattgaa 
      ccggcacat gtcatgaag aagcgaagt ccagaccag 
      tctttgaga attctgctt tattatgat tccagtgtg 
      tacagactc gaccgtgaa cggttcgac ttaatcaat 
      gaaacaatt ctgccgcaa gctgtcacc ttctgggag 
      aaggcattg tttgttcga agagttgag agtgtaata 
      cgattaacc aggaaatgc aacaacacg caggtgttc 
      gtgaagaac tccctgacc cactgcatc gacacttgc 
      aaggagaag acgatgtgc ggcgaagtg gtcgtacct 
      gaggagcat ttggacgcg tgcagaacc tgcaacgcc 
      acgggccaa gactgtggg atagcaaaa ggctcaaaa 
      gccggtgaa ggaatcgaa aatttcgat ttcgtcttt 
      tatgtaagc gccatgcag accgaacgt tgtaacaag 
      tcattaacc gtggcatat gccgcccac tgccaacaa 
      gaggcagct ttggacagg ccgatcgct gggcatgcc 
      aacctctgc ccaaacagc ataagtacc aagcggcaa 
      gaggtcgaa atcttgctt tcgacggtg aagcacgag 
      attttgcac gcgctgggc ttttcggta agtctgtac 
      gcgttctac cgggactcc aagggcgag cccttgacg 
      gagcgccgg ctggacacc gggaagccg cccctgaac 
      gagaccttg caaacgtac caatggagc gacaaggtg 
      attaggagt ttcgtgcgg aaaaactgg ctggtcaag 
      tcgggtgtg atgcgaaga gaggtgaat atgattgtc 
      acgcgaaac gtagtaagg gaagttcgt gattacttc 
      aaatgcgag gagctggaa ggggcagag ctggaagat 
      caaggtgag gaaggaacg gcgttgact cactgggaa 
      aagcgtgtg tttgaaaac gaggcaatg acggggacg 
      cacacccag aacccgatc atctcgcgc atcactctg 
      gccctgatg gaagacacc gggtggtac ctagccaac 
      tactcgatg gccgagccg atgtcgtgg ggcaaaggt 
      ctcggctgc gacttcgtc atgaaaagc tgcaaagag 
      tggataaca ataaagtct tcaagagga gcctcgatc 
      cacccattc tgcaataaa gtcaagcgt gatcctttg 
      caaacggaa tgcacagat gatcgtacg tcggttgcc 
      ctttgcaac ctggtcgct catgaaact aagcttcct 
      caattatat cagaatttt gattacatt aaatacgtc 
      gagccgggg accgaggag tattacggc gggtcggtc 
      gctctggcc gactactgc ccgtacatc caggaattc 
      acctggcgc tcaaagaac gtcattgtg cgaggatcg 
      cattgccag tacgccgag aataatccg aagcccgat 
      aagaatttc gctttagag aaatacggg gaaaactcg 
      cgttgcttt gaacatact aatcaaatg tgggaagag 
      agatcatgc ggccaaatg agacaatgg caacattgg 
      ggctcgggt tgctacggc taccgctgc cagcacgga 
      cgcctacat attttggtg gataattac tcgtacgag 
      tgcttccac ccgaatcaa gagatctcg atacgaatc 
      atcaacaac ggatggctt cacaaaggt gccatagtc 
      tgcccaccc tgccgggag ctctgcgct cgggagttt 
      gcccaacgg aacgaaagt tgcaaggga aacgacgag 
      gctcctcca gcaacggcg tatccaaga gacgagctt 
      aagtgttcc tcaaccatt ccaagacta agcggttcc 
      ctattgctc ctaattctg tttttctcc acatgtgcc 
      agagaacat ctagtctaa
    mRNA sequence
    • >TC014203 RA
      tcgttccag cagatgttg cgcgaggta gtgtagcta 
      acctcaaac gcgaatgtt tatattttt aaatttgat 
      ttcgtaaaa atggtggct ggagttaaa tggaaggac 
      gctttttgt gttattcta gtgacagtt tcattcaaa 
      tcttgggat aatttcgtg tatggacaa atttgtaat 
      catgtgcat cccaaacac gacgaggtg tctcacacg 
      cacattgaa ccggcacat gtcatgaag aagcgaagt 
      ccagaccag tctttgaga attctgctt tattatgat 
      tccagtgtg tacagactc gaccgtgaa cggttcgac 
      ttaatcaat gaaacaatt ctgccgcaa gctgtcacc 
      ttctgggag aaggcattg tttgttcga agagttgag 
      agtgtaata cgattaacc aggaaatgc aacaacacg 
      caggtgttc gtgaagaac tccctgacc cactgcatc 
      gacacttgc aaggagaag acgatgtgc ggcgaagtg 
      gtcgtacct gaggagcat ttggacgcg tgcagaacc 
      tgcaacgcc acgggccaa gactgtggg atagcaaaa 
      ggctcaaaa gccggtgaa ggaatcgaa aatttcgat 
      ttcgtcttt tatgtaagc gccatgcag accgaacgt 
      tgtaacaag tcattaacc gtggcatat gccgcccac 
      tgccaacaa gaggcagct ttggacagg ccgatcgct 
      gggcatgcc aacctctgc ccaaacagc ataagtacc 
      aagcggcaa gaggtcgaa atcttgctt tcgacggtg 
      aagcacgag attttgcac gcgctgggc ttttcggta 
      agtctgtac gcgttctac cgggactcc aagggcgag 
      cccttgacg gagcgccgg ctggacacc gggaagccg 
      cccctgaac gagaccttg caaacgtac caatggagc 
      gacaaggtg attaggagt ttcgtgcgg aaaaactgg 
      ctggtcaag tcgggtgtg atgcgaaga gaggtgaat 
      atgattgtc acgcgaaac gtagtaagg gaagttcgt 
      gattacttc aaatgcgag gagctggaa ggggcagag 
      ctggaagat caaggtgag gaaggaacg gcgttgact 
      cactgggaa aagcgtgtg tttgaaaac gaggcaatg 
      acggggacg cacacccag aacccgatc atctcgcgc 
      atcactctg gccctgatg gaagacacc gggtggtac 
      ctagccaac tactcgatg gccgagccg atgtcgtgg 
      ggcaaaggt ctcggctgc gacttcgtc atgaaaagc 
      tgcaaagag tggataaca ataaagtct tcaagagga 
      gcctcgatc cacccattc tgcaataaa gtcaagcgt 
      gatcctttg caaacggaa tgcacagat gatcgtacg 
      tcggttgcc ctttgcaac ctggtcgct catgaaact 
      aagcttcct caattatat cagaatttt gattacatt 
      aaatacgtc gagccgggg accgaggag tattacggc 
      gggtcggtc gctctggcc gactactgc ccgtacatc 
      caggaattc acctggcgc tcaaagaac gtcattgtg 
      cgaggatcg cattgccag tacgccgag aataatccg 
      aagcccgat aagaatttc gctttagag aaatacggg 
      gaaaactcg cgttgcttt gaacatact aatcaaatg 
      tgggaagag agatcatgc ggccaaatg agacaatgg 
      caacattgg ggctcgggt tgctacggc taccgctgc 
      cagcacgga cgcctacat attttggtg gataattac 
      tcgtacgag tgcttccac ccgaatcaa gagatctcg 
      atacgaatc atcaacaac ggatggctt cacaaaggt 
      gccatagtc tgcccaccc tgccgggag ctctgcgct 
      cgggagttt gcccaacgg aacgaaagt tgcaaggga 
      aacgacgag gctcctcca gcaacggcg tatccaaga 
      gacgagctt aagtgttcc tcaaccatt ccaagacta 
      agcggttcc ctattgctc ctaattctg tttttctcc 
      acatgtgcc agagaacat ctagtctaa tgtacttgg 
      aacgttttc aaacttatt tttctatgt ccaaatttg 
      gtatacatt ccaaaatta aatgaacat ggctgtgta 
      aaattattt taatttaaa acttttatt agacgtacc 
      taccgtaat aagtatttt taccttaat atcctagtg 
      tatatattt tctatcaac gaacattgt tttatcaag 
      ctttgtatt tattattaa tgtagtaat tctccaaaa 
      ataataaaa acattctac aaaa