Status
Please wait ...
Gene information
General information
TC014179 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2838016..2850640
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014179
    iBeetle screen iB_02257

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014179 RA
      MFGFQLILQ CSYMVVKIL VWSICLIAL CHAWMPDSS 
      SKLINHFKS VESKSFTGN ATFPDHFIV LNQDETSIL 
      VGGRNRVYN LSIFDLSER KGGRIDWPS SDAHGQLCI 
      LKGKTDDDC QNYIRILYS SEPGKLVIC GTNSYKPLC 
      RTYAFKEGK YLVEKEVEG IGLCPYNPE HNSTSVSYN 
      GQLFSATVA DFSGGDPLI YREPQRTEL SDLKQLNAP 
      NFVNSVAYG DYIFFFYRE TAVEYMNCG KVIYSRVAR 
      VCKDDKGGP HQSRDRWTS FLKARLNCS IPGEYPFYF 
      DEIQSTSDI VEGRYNSDD SKKIIYGIL TTPVNAIGG 
      SAICAYQMA DILRVFEGS FKHQETINS NWLPVPQNL 
      VPEPRPGQC VRDSRILPD KNVNFIKTH SLMEDAVPA 
      LFGKPVLVR VSLQYRFTA ITVDPQVKT INNQYLDVL 
      YIGTDDGKV LKAVNIPNA DTAKAIVIS ENTVLPHGA 
      PVKQLKIAP GYGKVVVVG KDEIRLANL NHCASKTRC 
      KDCVELQDP HCAWDAKQN LCVSIDTVT SYRFLIQDV 
      VRGDDNKCW SPQTDKKTV IKNKPSEVE NEITNSIDE 
      KDLDSSDPL IKTGLDDDS DCDPVSENS IGGCAVRQQ 
      LVIYTAGTL HIVVVVVSI VGLFLGFIA GYLFSQKFH 
      SHSQYPEAP FIEQHNHLE RLSANQTGY LTPRANKAV 
      NLVVNVSSS TPPPKKDNL DVSKDLNIA SDGTLQKIK 
      KTYI
    CDS sequence
    • >TC014179 RA
      atgtttggt ttccaattg atcttgcaa tgtagctac 
      atggtggtg aagatcttg gtttggtcg atatgtctg 
      atagcgctg tgtcatgct tggatgccg gatagttct 
      tccaaatta ataaaccat tttaaatca gttgaaagt 
      aaaagcttt accgggaac gccacgttc cctgatcac 
      tttattgtc ttgaatcaa gacgaaact tcgatatta 
      gtaggcggt agaaatagg gtttacaat ttaagtata 
      ttcgacctc agtgagcgt aaagggggg cgaatcgac 
      tggccatcg tccgatgca catggccag ttgtgtata 
      ttgaaaggg aaaacggac gacgactgc caaaattac 
      attagaata ctgtactct tcagaaccg gggaaatta 
      gttatttgc gggaccaat tcgtacaaa cccctctgt 
      cggacgtac gcatttaag gagggaaag tacctggtt 
      gagaaagaa gtagaaggg ataggcttg tgtccatac 
      aatccggaa cacaacagc acatctgtc tcctacaat 
      ggccaatta ttttcagcg acggtcgcc gacttttcc 
      gggggcgac cctctcata tacagggag ccccagcgc 
      accgaactc tcagatctc aaacaactg aacgcaccg 
      aatttcgta aactcggtg gcctatggc gactacata 
      ttcttcttc taccgtgaa accgccgtc gagtacatg 
      aactgcgga aaagtcatc tactcgcgg gtcgccagg 
      gtgtgcaag gacgacaaa gggggccct caccagtca 
      cgcgaccgc tggacgtcg ttcctcaaa gcacgtctc 
      aattgttca attcccggc gagtacccc ttttacttt 
      gatgaaatc caatcaaca agtgatata gtcgagggt 
      cggtacaat tccgacgac agcaaaaag atcatttat 
      ggaatcctc acaactcca gttaatgcc atcggcggc 
      tcggccatt tgcgcgtat caaatggcc gacatcttg 
      cgcgtgttt gaagggagc ttcaagcac caagagacg 
      atcaactcg aactggctc cccgtgccc cagaaccta 
      gtccctgaa cccaggccc gggcagtgc gtacgcgac 
      agcaggatc ctgcccgac aagaacgtc aactttatt 
      aagacccac tctttgatg gaggacgcc gttccggct 
      cttttcgga aaaccagtt ctggtccga gtgagtctg 
      cagtatcgg tttacagcc ataacagtg gatccacaa 
      gtgaaaaca atcaataat cagtatctc gatgttttg 
      tatatcgga acagatgat gggaaggta ctaaaagct 
      gttaatata ccaaacgcg gacaccgct aaagcgatt 
      gttatatcg gaaaatacc gtactaccg cacggagct 
      cccgtaaaa cagctgaag atcgctccc ggttatggc 
      aaagttgtg gtggtcggg aaagacgaa atcagactt 
      gctaatctc aaccattgt gcaagcaaa acgcggtgc 
      aaggactgt gtggaactg caagaccca cattgcgcc 
      tgggacgcc aaacaaaac ctgtgtgtc agcattgac 
      accgtcact tcgtatcgc ttcctgatc caggacgta 
      gttcgcggc gacgacaac aaatgttgg tcgccgcaa 
      acagacaaa aagactgtg attaagaat aagcccagc 
      gaggttgag aacgagatt acgaactcc attgacgaa 
      aaggatctc gattcaagc gatccgctc atcaaaact 
      ggtctcgat gacgattcc gattgtgat ccagtcagc 
      gagaacagc ataggcgga tgcgccgtc cgccagcaa 
      cttgttata tacacagct gggactcta cacattgtc 
      gtggtcgtc gtcagcatc gtgggttta tttcttggc 
      tttatagcg ggttatctg ttttcgcaa aaatttcac 
      tcgcattcg caatatcct gaggcgccg tttatagag 
      cagcacaat catttggaa agattaagc gccaaccag 
      acggggtat ttgactccg agggccaat aaagcggtc 
      aatttggtg gtgaacgtg tcgagtagc acgccgccg 
      ccgaaaaag gacaatctc gatgtcagc aaagacttg 
      aacattgcg agtgacggg actttgcaa aaaatcaag 
      aagacttac atttaa
    mRNA sequence
    • >TC014179 RA
      tggtttcag cctcggctt gtttggacc ccgacataa 
      tcttcgaac tacaatgaa gaggaaatt ttgaaacgc 
      gtttcagac gcgtacaat cgacaaaat gtttggttt 
      ccaattgat cttgcaatg tagctacat ggtggtgaa 
      gatcttggt ttggtcgat atgtctgat agcgctgtg 
      tcatgcttg gatgccgga tagttcttc caaattaat 
      aaaccattt taaatcagt tgaaagtaa aagctttac 
      cgggaacgc cacgttccc tgatcactt tattgtctt 
      gaatcaaga cgaaacttc gatattagt aggcggtag 
      aaatagggt ttacaattt aagtatatt cgacctcag 
      tgagcgtaa aggggggcg aatcgactg gccatcgtc 
      cgatgcaca tggccagtt gtgtatatt gaaagggaa 
      aacggacga cgactgcca aaattacat tagaatact 
      gtactcttc agaaccggg gaaattagt tatttgcgg 
      gaccaattc gtacaaacc cctctgtcg gacgtacgc 
      atttaagga gggaaagta cctggttga gaaagaagt 
      agaagggat aggcttgtg tccatacaa tccggaaca 
      caacagcac atctgtctc ctacaatgg ccaattatt 
      ttcagcgac ggtcgccga cttttccgg gggcgaccc 
      tctcatata cagggagcc ccagcgcac cgaactctc 
      agatctcaa acaactgaa cgcaccgaa tttcgtaaa 
      ctcggtggc ctatggcga ctacatatt cttcttcta 
      ccgtgaaac cgccgtcga gtacatgaa ctgcggaaa 
      agtcatcta ctcgcgggt cgccagggt gtgcaagga 
      cgacaaagg gggccctca ccagtcacg cgaccgctg 
      gacgtcgtt cctcaaagc acgtctcaa ttgttcaat 
      tcccggcga gtacccctt ttactttga tgaaatcca 
      atcaacaag tgatatagt cgagggtcg gtacaattc 
      cgacgacag caaaaagat catttatgg aatcctcac 
      aactccagt taatgccat cggcggctc ggccatttg 
      cgcgtatca aatggccga catcttgcg cgtgtttga 
      agggagctt caagcacca agagacgat caactcgaa 
      ctggctccc cgtgcccca gaacctagt ccctgaacc 
      caggcccgg gcagtgcgt acgcgacag caggatcct 
      gcccgacaa gaacgtcaa ctttattaa gacccactc 
      tttgatgga ggacgccgt tccggctct tttcggaaa 
      accagttct ggtccgagt gagtctgca gtatcggtt 
      tacagccat aacagtgga tccacaagt gaaaacaat 
      caataatca gtatctcga tgttttgta tatcggaac 
      agatgatgg gaaggtact aaaagctgt taatatacc 
      aaacgcgga caccgctaa agcgattgt tatatcgga 
      aaataccgt actaccgca cggagctcc cgtaaaaca 
      gctgaagat cgctcccgg ttatggcaa agttgtggt 
      ggtcgggaa agacgaaat cagacttgc taatctcaa 
      ccattgtgc aagcaaaac gcggtgcaa ggactgtgt 
      ggaactgca agacccaca ttgcgcctg ggacgccaa 
      acaaaacct gtgtgtcag cattgacac cgtcacttc 
      gtatcgctt cctgatcca ggacgtagt tcgcggcga 
      cgacaacaa atgttggtc gccgcaaac agacaaaaa 
      gactgtgat taagaataa gcccagcga ggttgagaa 
      cgagattac gaactccat tgacgaaaa ggatctcga 
      ttcaagcga tccgctcat caaaactgg tctcgatga 
      cgattccga ttgtgatcc agtcagcga gaacagcat 
      aggcggatg cgccgtccg ccagcaact tgttatata 
      cacagctgg gactctaca cattgtcgt ggtcgtcgt 
      cagcatcgt gggtttatt tcttggctt tatagcggg 
      ttatctgtt ttcgcaaaa atttcactc gcattcgca 
      atatcctga ggcgccgtt tatagagca gcacaatca 
      tttggaaag attaagcgc caaccagac ggggtattt 
      gactccgag ggccaataa agcggtcaa tttggtggt 
      gaacgtgtc gagtagcac gccgccgcc gaaaaagga 
      caatctcga tgtcagcaa agacttgaa cattgcgag 
      tgacgggac tttgcaaaa aatcaagaa gacttacat 
      ttaagtgcg actttttac tacgtgaat gtacctgtg 
      gtgatggag ggaggccat tccttgatg tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY