| General information | |||
| TC014179 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 2838016..2850640 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014179 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02257 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC014179 RA
MFGFQLILQ CSYMVVKIL VWSICLIAL CHAWMPDSS SKLINHFKS VESKSFTGN ATFPDHFIV LNQDETSIL VGGRNRVYN LSIFDLSER KGGRIDWPS SDAHGQLCI LKGKTDDDC QNYIRILYS SEPGKLVIC GTNSYKPLC RTYAFKEGK YLVEKEVEG IGLCPYNPE HNSTSVSYN GQLFSATVA DFSGGDPLI YREPQRTEL SDLKQLNAP NFVNSVAYG DYIFFFYRE TAVEYMNCG KVIYSRVAR VCKDDKGGP HQSRDRWTS FLKARLNCS IPGEYPFYF DEIQSTSDI VEGRYNSDD SKKIIYGIL TTPVNAIGG SAICAYQMA DILRVFEGS FKHQETINS NWLPVPQNL VPEPRPGQC VRDSRILPD KNVNFIKTH SLMEDAVPA LFGKPVLVR VSLQYRFTA ITVDPQVKT INNQYLDVL YIGTDDGKV LKAVNIPNA DTAKAIVIS ENTVLPHGA PVKQLKIAP GYGKVVVVG KDEIRLANL NHCASKTRC KDCVELQDP HCAWDAKQN LCVSIDTVT SYRFLIQDV VRGDDNKCW SPQTDKKTV IKNKPSEVE NEITNSIDE KDLDSSDPL IKTGLDDDS DCDPVSENS IGGCAVRQQ LVIYTAGTL HIVVVVVSI VGLFLGFIA GYLFSQKFH SHSQYPEAP FIEQHNHLE RLSANQTGY LTPRANKAV NLVVNVSSS TPPPKKDNL DVSKDLNIA SDGTLQKIK KTYI
- >TC014179 RA
atgtttggt ttccaattg atcttgcaa tgtagctac atggtggtg aagatcttg gtttggtcg atatgtctg atagcgctg tgtcatgct tggatgccg gatagttct tccaaatta ataaaccat tttaaatca gttgaaagt aaaagcttt accgggaac gccacgttc cctgatcac tttattgtc ttgaatcaa gacgaaact tcgatatta gtaggcggt agaaatagg gtttacaat ttaagtata ttcgacctc agtgagcgt aaagggggg cgaatcgac tggccatcg tccgatgca catggccag ttgtgtata ttgaaaggg aaaacggac gacgactgc caaaattac attagaata ctgtactct tcagaaccg gggaaatta gttatttgc gggaccaat tcgtacaaa cccctctgt cggacgtac gcatttaag gagggaaag tacctggtt gagaaagaa gtagaaggg ataggcttg tgtccatac aatccggaa cacaacagc acatctgtc tcctacaat ggccaatta ttttcagcg acggtcgcc gacttttcc gggggcgac cctctcata tacagggag ccccagcgc accgaactc tcagatctc aaacaactg aacgcaccg aatttcgta aactcggtg gcctatggc gactacata ttcttcttc taccgtgaa accgccgtc gagtacatg aactgcgga aaagtcatc tactcgcgg gtcgccagg gtgtgcaag gacgacaaa gggggccct caccagtca cgcgaccgc tggacgtcg ttcctcaaa gcacgtctc aattgttca attcccggc gagtacccc ttttacttt gatgaaatc caatcaaca agtgatata gtcgagggt cggtacaat tccgacgac agcaaaaag atcatttat ggaatcctc acaactcca gttaatgcc atcggcggc tcggccatt tgcgcgtat caaatggcc gacatcttg cgcgtgttt gaagggagc ttcaagcac caagagacg atcaactcg aactggctc cccgtgccc cagaaccta gtccctgaa cccaggccc gggcagtgc gtacgcgac agcaggatc ctgcccgac aagaacgtc aactttatt aagacccac tctttgatg gaggacgcc gttccggct cttttcgga aaaccagtt ctggtccga gtgagtctg cagtatcgg tttacagcc ataacagtg gatccacaa gtgaaaaca atcaataat cagtatctc gatgttttg tatatcgga acagatgat gggaaggta ctaaaagct gttaatata ccaaacgcg gacaccgct aaagcgatt gttatatcg gaaaatacc gtactaccg cacggagct cccgtaaaa cagctgaag atcgctccc ggttatggc aaagttgtg gtggtcggg aaagacgaa atcagactt gctaatctc aaccattgt gcaagcaaa acgcggtgc aaggactgt gtggaactg caagaccca cattgcgcc tgggacgcc aaacaaaac ctgtgtgtc agcattgac accgtcact tcgtatcgc ttcctgatc caggacgta gttcgcggc gacgacaac aaatgttgg tcgccgcaa acagacaaa aagactgtg attaagaat aagcccagc gaggttgag aacgagatt acgaactcc attgacgaa aaggatctc gattcaagc gatccgctc atcaaaact ggtctcgat gacgattcc gattgtgat ccagtcagc gagaacagc ataggcgga tgcgccgtc cgccagcaa cttgttata tacacagct gggactcta cacattgtc gtggtcgtc gtcagcatc gtgggttta tttcttggc tttatagcg ggttatctg ttttcgcaa aaatttcac tcgcattcg caatatcct gaggcgccg tttatagag cagcacaat catttggaa agattaagc gccaaccag acggggtat ttgactccg agggccaat aaagcggtc aatttggtg gtgaacgtg tcgagtagc acgccgccg ccgaaaaag gacaatctc gatgtcagc aaagacttg aacattgcg agtgacggg actttgcaa aaaatcaag aagacttac atttaa
- >TC014179 RA
tggtttcag cctcggctt gtttggacc ccgacataa tcttcgaac tacaatgaa gaggaaatt ttgaaacgc gtttcagac gcgtacaat cgacaaaat gtttggttt ccaattgat cttgcaatg tagctacat ggtggtgaa gatcttggt ttggtcgat atgtctgat agcgctgtg tcatgcttg gatgccgga tagttcttc caaattaat aaaccattt taaatcagt tgaaagtaa aagctttac cgggaacgc cacgttccc tgatcactt tattgtctt gaatcaaga cgaaacttc gatattagt aggcggtag aaatagggt ttacaattt aagtatatt cgacctcag tgagcgtaa aggggggcg aatcgactg gccatcgtc cgatgcaca tggccagtt gtgtatatt gaaagggaa aacggacga cgactgcca aaattacat tagaatact gtactcttc agaaccggg gaaattagt tatttgcgg gaccaattc gtacaaacc cctctgtcg gacgtacgc atttaagga gggaaagta cctggttga gaaagaagt agaagggat aggcttgtg tccatacaa tccggaaca caacagcac atctgtctc ctacaatgg ccaattatt ttcagcgac ggtcgccga cttttccgg gggcgaccc tctcatata cagggagcc ccagcgcac cgaactctc agatctcaa acaactgaa cgcaccgaa tttcgtaaa ctcggtggc ctatggcga ctacatatt cttcttcta ccgtgaaac cgccgtcga gtacatgaa ctgcggaaa agtcatcta ctcgcgggt cgccagggt gtgcaagga cgacaaagg gggccctca ccagtcacg cgaccgctg gacgtcgtt cctcaaagc acgtctcaa ttgttcaat tcccggcga gtacccctt ttactttga tgaaatcca atcaacaag tgatatagt cgagggtcg gtacaattc cgacgacag caaaaagat catttatgg aatcctcac aactccagt taatgccat cggcggctc ggccatttg cgcgtatca aatggccga catcttgcg cgtgtttga agggagctt caagcacca agagacgat caactcgaa ctggctccc cgtgcccca gaacctagt ccctgaacc caggcccgg gcagtgcgt acgcgacag caggatcct gcccgacaa gaacgtcaa ctttattaa gacccactc tttgatgga ggacgccgt tccggctct tttcggaaa accagttct ggtccgagt gagtctgca gtatcggtt tacagccat aacagtgga tccacaagt gaaaacaat caataatca gtatctcga tgttttgta tatcggaac agatgatgg gaaggtact aaaagctgt taatatacc aaacgcgga caccgctaa agcgattgt tatatcgga aaataccgt actaccgca cggagctcc cgtaaaaca gctgaagat cgctcccgg ttatggcaa agttgtggt ggtcgggaa agacgaaat cagacttgc taatctcaa ccattgtgc aagcaaaac gcggtgcaa ggactgtgt ggaactgca agacccaca ttgcgcctg ggacgccaa acaaaacct gtgtgtcag cattgacac cgtcacttc gtatcgctt cctgatcca ggacgtagt tcgcggcga cgacaacaa atgttggtc gccgcaaac agacaaaaa gactgtgat taagaataa gcccagcga ggttgagaa cgagattac gaactccat tgacgaaaa ggatctcga ttcaagcga tccgctcat caaaactgg tctcgatga cgattccga ttgtgatcc agtcagcga gaacagcat aggcggatg cgccgtccg ccagcaact tgttatata cacagctgg gactctaca cattgtcgt ggtcgtcgt cagcatcgt gggtttatt tcttggctt tatagcggg ttatctgtt ttcgcaaaa atttcactc gcattcgca atatcctga ggcgccgtt tatagagca gcacaatca tttggaaag attaagcgc caaccagac ggggtattt gactccgag ggccaataa agcggtcaa tttggtggt gaacgtgtc gagtagcac gccgccgcc gaaaaagga caatctcga tgtcagcaa agacttgaa cattgcgag tgacgggac tttgcaaaa aatcaagaa gacttacat ttaagtgcg actttttac tacgtgaat gtacctgtg gtgatggag ggaggccat tccttgatg tt
