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Gene information
General information
TC014179 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2838016..2850640
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014179
    iBeetle screen iB_02257

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014179 RA
      MFGFQLILQ CSYMVVKIL VWSICLIAL CHAWMPDSS 
SKLINHFKS VESKSFTGN ATFPDHFIV LNQDETSIL 
VGGRNRVYN LSIFDLSER KGGRIDWPS SDAHGQLCI 
LKGKTDDDC QNYIRILYS SEPGKLVIC GTNSYKPLC 
RTYAFKEGK YLVEKEVEG IGLCPYNPE HNSTSVSYN 
GQLFSATVA DFSGGDPLI YREPQRTEL SDLKQLNAP 
NFVNSVAYG DYIFFFYRE TAVEYMNCG KVIYSRVAR 
VCKDDKGGP HQSRDRWTS FLKARLNCS IPGEYPFYF 
DEIQSTSDI VEGRYNSDD SKKIIYGIL TTPVNAIGG 
SAICAYQMA DILRVFEGS FKHQETINS NWLPVPQNL 
VPEPRPGQC VRDSRILPD KNVNFIKTH SLMEDAVPA 
LFGKPVLVR VSLQYRFTA ITVDPQVKT INNQYLDVL 
YIGTDDGKV LKAVNIPNA DTAKAIVIS ENTVLPHGA 
PVKQLKIAP GYGKVVVVG KDEIRLANL NHCASKTRC 
KDCVELQDP HCAWDAKQN LCVSIDTVT SYRFLIQDV 
VRGDDNKCW SPQTDKKTV IKNKPSEVE NEITNSIDE 
KDLDSSDPL IKTGLDDDS DCDPVSENS IGGCAVRQQ 
LVIYTAGTL HIVVVVVSI VGLFLGFIA GYLFSQKFH 
SHSQYPEAP FIEQHNHLE RLSANQTGY LTPRANKAV 
NLVVNVSSS TPPPKKDNL DVSKDLNIA SDGTLQKIK 
KTYI
    CDS sequence
    • >TC014179 RA
      atgtttggt ttccaattg atcttgcaa tgtagctac 
atggtggtg aagatcttg gtttggtcg atatgtctg 
atagcgctg tgtcatgct tggatgccg gatagttct 
tccaaatta ataaaccat tttaaatca gttgaaagt 
aaaagcttt accgggaac gccacgttc cctgatcac 
tttattgtc ttgaatcaa gacgaaact tcgatatta 
gtaggcggt agaaatagg gtttacaat ttaagtata 
ttcgacctc agtgagcgt aaagggggg cgaatcgac 
tggccatcg tccgatgca catggccag ttgtgtata 
ttgaaaggg aaaacggac gacgactgc caaaattac 
attagaata ctgtactct tcagaaccg gggaaatta 
gttatttgc gggaccaat tcgtacaaa cccctctgt 
cggacgtac gcatttaag gagggaaag tacctggtt 
gagaaagaa gtagaaggg ataggcttg tgtccatac 
aatccggaa cacaacagc acatctgtc tcctacaat 
ggccaatta ttttcagcg acggtcgcc gacttttcc 
gggggcgac cctctcata tacagggag ccccagcgc 
accgaactc tcagatctc aaacaactg aacgcaccg 
aatttcgta aactcggtg gcctatggc gactacata 
ttcttcttc taccgtgaa accgccgtc gagtacatg 
aactgcgga aaagtcatc tactcgcgg gtcgccagg 
gtgtgcaag gacgacaaa gggggccct caccagtca 
cgcgaccgc tggacgtcg ttcctcaaa gcacgtctc 
aattgttca attcccggc gagtacccc ttttacttt 
gatgaaatc caatcaaca agtgatata gtcgagggt 
cggtacaat tccgacgac agcaaaaag atcatttat 
ggaatcctc acaactcca gttaatgcc atcggcggc 
tcggccatt tgcgcgtat caaatggcc gacatcttg 
cgcgtgttt gaagggagc ttcaagcac caagagacg 
atcaactcg aactggctc cccgtgccc cagaaccta 
gtccctgaa cccaggccc gggcagtgc gtacgcgac 
agcaggatc ctgcccgac aagaacgtc aactttatt 
aagacccac tctttgatg gaggacgcc gttccggct 
cttttcgga aaaccagtt ctggtccga gtgagtctg 
cagtatcgg tttacagcc ataacagtg gatccacaa 
gtgaaaaca atcaataat cagtatctc gatgttttg 
tatatcgga acagatgat gggaaggta ctaaaagct 
gttaatata ccaaacgcg gacaccgct aaagcgatt 
gttatatcg gaaaatacc gtactaccg cacggagct 
cccgtaaaa cagctgaag atcgctccc ggttatggc 
aaagttgtg gtggtcggg aaagacgaa atcagactt 
gctaatctc aaccattgt gcaagcaaa acgcggtgc 
aaggactgt gtggaactg caagaccca cattgcgcc 
tgggacgcc aaacaaaac ctgtgtgtc agcattgac 
accgtcact tcgtatcgc ttcctgatc caggacgta 
gttcgcggc gacgacaac aaatgttgg tcgccgcaa 
acagacaaa aagactgtg attaagaat aagcccagc 
gaggttgag aacgagatt acgaactcc attgacgaa 
aaggatctc gattcaagc gatccgctc atcaaaact 
ggtctcgat gacgattcc gattgtgat ccagtcagc 
gagaacagc ataggcgga tgcgccgtc cgccagcaa 
cttgttata tacacagct gggactcta cacattgtc 
gtggtcgtc gtcagcatc gtgggttta tttcttggc 
tttatagcg ggttatctg ttttcgcaa aaatttcac 
tcgcattcg caatatcct gaggcgccg tttatagag 
cagcacaat catttggaa agattaagc gccaaccag 
acggggtat ttgactccg agggccaat aaagcggtc 
aatttggtg gtgaacgtg tcgagtagc acgccgccg 
ccgaaaaag gacaatctc gatgtcagc aaagacttg 
aacattgcg agtgacggg actttgcaa aaaatcaag 
aagacttac atttaa
    mRNA sequence
    • >TC014179 RA
      tggtttcag cctcggctt gtttggacc ccgacataa 
tcttcgaac tacaatgaa gaggaaatt ttgaaacgc 
gtttcagac gcgtacaat cgacaaaat gtttggttt 
ccaattgat cttgcaatg tagctacat ggtggtgaa 
gatcttggt ttggtcgat atgtctgat agcgctgtg 
tcatgcttg gatgccgga tagttcttc caaattaat 
aaaccattt taaatcagt tgaaagtaa aagctttac 
cgggaacgc cacgttccc tgatcactt tattgtctt 
gaatcaaga cgaaacttc gatattagt aggcggtag 
aaatagggt ttacaattt aagtatatt cgacctcag 
tgagcgtaa aggggggcg aatcgactg gccatcgtc 
cgatgcaca tggccagtt gtgtatatt gaaagggaa 
aacggacga cgactgcca aaattacat tagaatact 
gtactcttc agaaccggg gaaattagt tatttgcgg 
gaccaattc gtacaaacc cctctgtcg gacgtacgc 
atttaagga gggaaagta cctggttga gaaagaagt 
agaagggat aggcttgtg tccatacaa tccggaaca 
caacagcac atctgtctc ctacaatgg ccaattatt 
ttcagcgac ggtcgccga cttttccgg gggcgaccc 
tctcatata cagggagcc ccagcgcac cgaactctc 
agatctcaa acaactgaa cgcaccgaa tttcgtaaa 
ctcggtggc ctatggcga ctacatatt cttcttcta 
ccgtgaaac cgccgtcga gtacatgaa ctgcggaaa 
agtcatcta ctcgcgggt cgccagggt gtgcaagga 
cgacaaagg gggccctca ccagtcacg cgaccgctg 
gacgtcgtt cctcaaagc acgtctcaa ttgttcaat 
tcccggcga gtacccctt ttactttga tgaaatcca 
atcaacaag tgatatagt cgagggtcg gtacaattc 
cgacgacag caaaaagat catttatgg aatcctcac 
aactccagt taatgccat cggcggctc ggccatttg 
cgcgtatca aatggccga catcttgcg cgtgtttga 
agggagctt caagcacca agagacgat caactcgaa 
ctggctccc cgtgcccca gaacctagt ccctgaacc 
caggcccgg gcagtgcgt acgcgacag caggatcct 
gcccgacaa gaacgtcaa ctttattaa gacccactc 
tttgatgga ggacgccgt tccggctct tttcggaaa 
accagttct ggtccgagt gagtctgca gtatcggtt 
tacagccat aacagtgga tccacaagt gaaaacaat 
caataatca gtatctcga tgttttgta tatcggaac 
agatgatgg gaaggtact aaaagctgt taatatacc 
aaacgcgga caccgctaa agcgattgt tatatcgga 
aaataccgt actaccgca cggagctcc cgtaaaaca 
gctgaagat cgctcccgg ttatggcaa agttgtggt 
ggtcgggaa agacgaaat cagacttgc taatctcaa 
ccattgtgc aagcaaaac gcggtgcaa ggactgtgt 
ggaactgca agacccaca ttgcgcctg ggacgccaa 
acaaaacct gtgtgtcag cattgacac cgtcacttc 
gtatcgctt cctgatcca ggacgtagt tcgcggcga 
cgacaacaa atgttggtc gccgcaaac agacaaaaa 
gactgtgat taagaataa gcccagcga ggttgagaa 
cgagattac gaactccat tgacgaaaa ggatctcga 
ttcaagcga tccgctcat caaaactgg tctcgatga 
cgattccga ttgtgatcc agtcagcga gaacagcat 
aggcggatg cgccgtccg ccagcaact tgttatata 
cacagctgg gactctaca cattgtcgt ggtcgtcgt 
cagcatcgt gggtttatt tcttggctt tatagcggg 
ttatctgtt ttcgcaaaa atttcactc gcattcgca 
atatcctga ggcgccgtt tatagagca gcacaatca 
tttggaaag attaagcgc caaccagac ggggtattt 
gactccgag ggccaataa agcggtcaa tttggtggt 
gaacgtgtc gagtagcac gccgccgcc gaaaaagga 
caatctcga tgtcagcaa agacttgaa cattgcgag 
tgacgggac tttgcaaaa aatcaagaa gacttacat 
ttaagtgcg actttttac tacgtgaat gtacctgtg 
gtgatggag ggaggccat tccttgatg tt
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