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Gene information
General information
TC014168 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 2295774..2333954
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014168
    iBeetle screen iB_02256
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014168 RA
      MDFIILKVN GTDVSSFPH EDAVRAFLT AQEPIVVEV 
      KRRISESPQ EKTPTSKLI STGCQTELS GLTWLEDNS 
      LDCLTHDID LEEVTLRKC SSDEKLGLT VCYSSGSEA 
      DTCTEVYIR DIAPQSVAD RDGRLRQGD QILQVNGKD 
      VANKEETES LFAENRKAV TLLVSRCYN RSEEYLDAE 
      PIDAISPGG RSAAYQNSL IEQLVQQQT ESQHSPRND 
      TAPKVPPHF IQDRLNLTN SLVRSKMDS INHEISELD 
      HRMQNIQLV KIDKRKPPQ LPHIPAPQE SDTEHIYET 
      IPESVDSEM EPIYSCPYE ADEQSMVEQ WLKLQDQGW 
      NPQNKDATP KDNKKQRCK SSKSNSSGE EHENSSSAY 
      NTGGSCNSN PLTFELACS QDSKQKDTY RSTLILCPP 
      VEKEDTSKE KDQTETCDA CKQQATSKK KSKSTSKTN 
      SPSSPTTRI GVGATPAHL LSDTMYTNV ANLQQTMLL 
      QQQLFRQAL VQNNIDIAS KPTTSFTSP SLSQYQFVS 
      SSQTYTTQP ETATLETMM EWKVKRRPD GTRYIARRP 
      VRNRILKNR AIKISEERA GLTTEDDTV SELKIGRYW 
      TKEERKKHL EKSKERKQR QEILLASRN IEENGELVI 
      HKPVEKVHS FSSKKAANN LDNTVKKHK SKRAHKEGY 
      DNFPTVQEM IVHGPKVPT NGKMMGLLS VTTV
    • >TC014168 RB
      MSIFIIVEV TLRKCSSDE KLGLTVCYS SGSEADTCT 
      EVYIRDIAP QSVADRDGR LRQGDQILQ VNGKDVANK 
      EETESLFAE NRKAVTLLV SRCYNRSEE YLDAEPIDA 
      ISPGGRSAA YQNSLIEQL VQQQTESQH SPRNDTAPK 
      VPPHFIQDR LNLTNSLVR SKMDSINHE ISELDHRMQ 
      NIQLVKIDK RKPPQLPHI PAPQESDTE HIYETIPES 
      VDSEMEPIY SCPYEADEQ SMVEQWLKL QDQGWNPQN 
      KDATPKDNK KQRCKSSKS NSSGEEHEN SSSAYNTGG 
      SCNSNPLTF ELACSQDSK QKDTYRSTL ILCPPVEKE 
      DTSKEKDQT ETCDACKQQ ATSKKKSKS TSKTNSPSS 
      PTTRIGVGA TPAHLLSDT MYTNVANLQ QTMLLQQQL 
      FRQALVQNN IDIASKPTT SFTSPSLSQ YQFVSSSQT 
      YTTQPETAT LETMMEWKV KRRPDGTRY IARRPVRNR 
      ILKNRAIKI SEERAGLTT EDDTVSELK IGRYWTKEE 
      RKKHLEKSK ERKQRQEIL LASRNIEEN GELVIHKPV 
      EKVHSFSSK KAANNLDNT VKKHKSKRA HKEGYDNFP 
      TVQEMIVHG PKVPTNGKM MGLLSVTTV
    CDS sequence
    • >TC014168 RA
      atggatttt atcatactc aaggtcaat gggactgac 
      gtaagcagc ttcccacac gaagatgca gttagagca 
      tttctcacc gcacaagaa cctattgtt gttgaagtt 
      aagagaaga atatcggag tcgccacaa gaaaaaact 
      cccacttct aaactgatc tcaacaggt tgccaaaca 
      gaactttcg ggattgaca tggctggaa gataactct 
      ttggactgt ttgacacac gatatagac ttggaggaa 
      gtgacgtta cgaaaatgt agtagcgac gagaagtta 
      ggtttaaca gtttgctac agctccggt tctgaagct 
      gacacttgc acggaagtg tatattaga gatatagcg 
      ccccaaagt gttgccgac agggatggc agattacga 
      caaggcgat caaatttta caagtcaat ggaaaagac 
      gtcgctaat aaagaagaa actgaatcc ctcttcgct 
      gaaaataga aaagccgtc acattattg gttagcaga 
      tgctataac aggagcgaa gaatacttg gatgccgaa 
      ccgatcgac gctatttca ccaggaggt cgcagtgcc 
      gcctaccaa aacagtctt atagaacaa ttagtacaa 
      cagcagaca gaatcacag cattcccct agaaacgac 
      acagccccc aaagttcct cctcacttc atccaagac 
      cgacttaat ctcaccaat agtttagta cgttcgaaa 
      atggactca ataaatcac gaaatttcc gagttagac 
      caccgcatg caaaacatc cagttagta aaaatcgac 
      aagcggaaa cctccgcag ttaccacac atcccagca 
      cctcaagaa tccgacacg gaacacata tatgaaacg 
      atcccagag tcggtagat tccgaaatg gaacccatc 
      tattcttgc ccatacgaa gccgacgaa caaagcatg 
      gtggaacaa tggctgaaa ctgcaagac caaggatgg 
      aacccgcaa aataaagac gcaacaccc aaagataat 
      aagaaacaa aggtgcaag tcatcgaag agcaacagc 
      agtggtgag gaacatgaa aatagctcc agtgcttat 
      aacacggga ggatcttgt aacagtaat cctttaact 
      ttcgagcta gcctgtagc caggattcg aaacaaaag 
      gatacctat agatccacg ttaattttg tgtcccccc 
      gtggagaag gaagacacc tcgaaggaa aaagaccag 
      acggaaacg tgcgatgcg tgcaaacag caagcaacc 
      tccaaaaag aaatctaag tcaacgtca aaaacgaat 
      agcccgtct tctcccacg actagaata ggagttgga 
      gcaactcca gcgcatctg ctctctgac accatgtac 
      acaaacgta gctaatttg caacaaact atgctcctg 
      cagcagcaa ctgtttaga caagcttta gttcaaaat 
      aatattgac atagcgtcg aaacctacg acaagcttc 
      acatccccg agcttaagt cagtaccaa ttcgtcagt 
      agctcacag acttacacc acccaaccc gagactgcc 
      actttagaa acaatgatg gaatggaag gtcaagcgc 
      agacctgac ggtaccagg tatatagcg cggcggccg 
      gttcggaat aggatactt aaaaataga gcgatcaaa 
      atatcggaa gaaagggcg ggattaacg acggaagac 
      gacactgtg tcggaactc aaaattggg cggtactgg 
      acgaaggag gagcgcaaa aaacattta gaaaagtcg 
      aaggagagg aagcagagg caggagata ttgctagcg 
      tcgaggaac atcgaggaa aacggcgaa ttagttata 
      cataaaccg gtcgagaaa gttcactcg ttttcgtcg 
      aagaaagcc gcgaacaat ttagataat actgtgaaa 
      aaacataaa agcaaacgg gcccacaaa gaaggatat 
      gataatttt ccaacagtg caagaaatg attgtccac 
      ggacccaaa gtccccacc aacggcaag atgatggga 
      ctgctctcc gtgacaacg gtttaa
    • >TC014168 RB
      atgtccatc ttcatcatc gttgaagtg acgttacga 
      aaatgtagt agcgacgag aagttaggt ttaacagtt 
      tgctacagc tccggttct gaagctgac acttgcacg 
      gaagtgtat attagagat atagcgccc caaagtgtt 
      gccgacagg gatggcaga ttacgacaa ggcgatcaa 
      attttacaa gtcaatgga aaagacgtc gctaataaa 
      gaagaaact gaatccctc ttcgctgaa aatagaaaa 
      gccgtcaca ttattggtt agcagatgc tataacagg 
      agcgaagaa tacttggat gccgaaccg atcgacgct 
      atttcacca ggaggtcgc agtgccgcc taccaaaac 
      agtcttata gaacaatta gtacaacag cagacagaa 
      tcacagcat tcccctaga aacgacaca gcccccaaa 
      gttcctcct cacttcatc caagaccga cttaatctc 
      accaatagt ttagtacgt tcgaaaatg gactcaata 
      aatcacgaa atttccgag ttagaccac cgcatgcaa 
      aacatccag ttagtaaaa atcgacaag cggaaacct 
      ccgcagtta ccacacatc ccagcacct caagaatcc 
      gacacggaa cacatatat gaaacgatc ccagagtcg 
      gtagattcc gaaatggaa cccatctat tcttgccca 
      tacgaagcc gacgaacaa agcatggtg gaacaatgg 
      ctgaaactg caagaccaa ggatggaac ccgcaaaat 
      aaagacgca acacccaaa gataataag aaacaaagg 
      tgcaagtca tcgaagagc aacagcagt ggtgaggaa 
      catgaaaat agctccagt gcttataac acgggagga 
      tcttgtaac agtaatcct ttaactttc gagctagcc 
      tgtagccag gattcgaaa caaaaggat acctataga 
      tccacgtta attttgtgt ccccccgtg gagaaggaa 
      gacacctcg aaggaaaaa gaccagacg gaaacgtgc 
      gatgcgtgc aaacagcaa gcaacctcc aaaaagaaa 
      tctaagtca acgtcaaaa acgaatagc ccgtcttct 
      cccacgact agaatagga gttggagca actccagcg 
      catctgctc tctgacacc atgtacaca aacgtagct 
      aatttgcaa caaactatg ctcctgcag cagcaactg 
      tttagacaa gctttagtt caaaataat attgacata 
      gcgtcgaaa cctacgaca agcttcaca tccccgagc 
      ttaagtcag taccaattc gtcagtagc tcacagact 
      tacaccacc caacccgag actgccact ttagaaaca 
      atgatggaa tggaaggtc aagcgcaga cctgacggt 
      accaggtat atagcgcgg cggccggtt cggaatagg 
      atacttaaa aatagagcg atcaaaata tcggaagaa 
      agggcggga ttaacgacg gaagacgac actgtgtcg 
      gaactcaaa attgggcgg tactggacg aaggaggag 
      cgcaaaaaa catttagaa aagtcgaag gagaggaag 
      cagaggcag gagatattg ctagcgtcg aggaacatc 
      gaggaaaac ggcgaatta gttatacat aaaccggtc 
      gagaaagtt cactcgttt tcgtcgaag aaagccgcg 
      aacaattta gataatact gtgaaaaaa cataaaagc 
      aaacgggcc cacaaagaa ggatatgat aattttcca 
      acagtgcaa gaaatgatt gtccacgga cccaaagtc 
      cccaccaac ggcaagatg atgggactg ctctccgtg 
      acaacggtt taa
    mRNA sequence
    • >TC014168 RA
      cgcgagata ataacaaac aggtgataa ttgaattgt 
      tcgtactga taagcaccg cagttgact tattgatta 
      cctatcggc agtcttcac tgacttctc ggagaagac 
      attgtgacc ttctgtgac ccacttaat tcggacaaa 
      tttaaactg tagtttttg tcagtgtta cctcccaag 
      aacggtacg tacattatt tttcacact tcacatcaa 
      taaatcaac accaaacaa ctccagtct attcaagtg 
      agcagatgt cttcgacta gttaaacaa atataatca 
      actcgaaat tttatatac accatccga aataaatca 
      aaaaaattc ttctagcgt aactataga ttcgtttcc 
      cgaaatgtt tatctaact cgctctcgt cccgatatt 
      ctttacgga aggagtaaa aagtatagt gcaaaaaca 
      ctctcctag aaagttttt ggtctgaat tcagccggg 
      cttagtcta tttattata attcaaagc atgttgtga 
      ctgtcggtc gatatgaca aagaaagaa tgcaacaaa 
      aattcattc atcattctt taatcatgc aaagtatag 
      aacagacca taatacgcg aaaaccaac gatttgagc 
      gaaaatatg ttcttctaa ttccgccat ttccatggt 
      ggttatatt gagaatcgc ttagcaagg caactaatt 
      aaacatcac agtgggaac aattgaatt ttaagtcga 
      tttagacgt ataattact tttactgtc ataaccatc 
      ctgagcgtc catcgtttc gattaagcc ctgacaatt 
      aacagctat caagggtat ttaccattc ggtttcgat 
      aagcaatac ttaggtatt aagtggtca tcaggatgc 
      tgtgacgtt accatttca gaaactttg tactgaatc 
      cagattttt tgtcaaagt ggtgcagat tgcagtaat 
      tatcactgt gtattacac cacaaagac aacttgcaa 
      ccgctacca aatctttaa agtggaaaa tggcattac 
      aaagtggcg tggaaatat aatcaactg aagtgagat 
      atacggtat ctaattaat cgcaagcca tgtagaaca 
      gcatgttat gcaatttcc ggaaaaaat gtggatctg 
      aagtgcact tggtggaca cccacatcg tccagaatc 
      catcagatt tgacgtttt tatgtgttc aaacactta 
      gcccaataa attaatttc gggatctgg gtccgaagt 
      gcaagttgt ccaggtgcc cgccggcaa tagtccggt 
      tgttgttgg ttttattgg cgtgttgtt gatttcgga 
      gttccctcg gaagtgaag ggtctgagt tattgttgt 
      gcttgtgcc ggtggtccc ggtgtcgaa cgtttgaaa 
      ttgggtgtg ttgatatgt tgattggtg gtgaggcat 
      tgtgtgcgt taattgctt ctgaggtcc gggcgcggg 
      tccgaaaga gtcctaatt gaattaaac gtgtcgcgg 
      catccggaa tgcgaccgg gcagcccct gaaccttgc 
      catagctat caacaatat aagataatg aacgcgtat 
      tcctgatga agagcaggg gggcgctgg ggtgcagac 
      tgcagcaca agtacagca cggagtcgc aaccacggc 
      gttactcag ttggaattc gtgcctcag ctttggtgg 
      ctgcagccg ttcagtggc ctcagtgct cgcaatgcg 
      ctcgctctc cacggacga ctctgcgga ctccaacac 
      acgcgttgt ctcggtgcc tctcgttcc gatgcgatc 
      gacttgtgc ttgcaattg aacgcaaca tacatggat 
      tttatcata ctcaaggtc aatgggact gacgtaagc 
      agcttccca cacgaagat gcagttaga gcatttctc 
      accgcacaa gaacctatt gttgttgaa gttaagaga 
      agaatatcg gagtcgcca caagaaaaa actcccact 
      tctaaactg atctcaaca ggttgccaa acagaactt 
      tcgggattg acatggctg gaagataac tctttggac 
      tgtttgaca cacgatata gacttggag gaagtgacg 
      ttacgaaaa tgtagtagc gacgagaag ttaggttta 
      acagtttgc tacagctcc ggttctgaa gctgacact 
      tgcacggaa gtgtatatt agagatata gcgccccaa 
      agtgttgcc gacagggat ggcagatta cgacaaggc 
      gatcaaatt ttacaagtc aatggaaaa gacgtcgct 
      aataaagaa gaaactgaa tccctcttc gctgaaaat 
      agaaaagcc gtcacatta ttggttagc agatgctat 
      aacaggagc gaagaatac ttggatgcc gaaccgatc 
      gacgctatt tcaccagga ggtcgcagt gccgcctac 
      caaaacagt cttatagaa caattagta caacagcag 
      acagaatca cagcattcc cctagaaac gacacagcc 
      cccaaagtt cctcctcac ttcatccaa gaccgactt 
      aatctcacc aatagttta gtacgttcg aaaatggac 
      tcaataaat cacgaaatt tccgagtta gaccaccgc 
      atgcaaaac atccagtta gtaaaaatc gacaagcgg 
      aaacctccg cagttacca cacatccca gcacctcaa 
      gaatccgac acggaacac atatatgaa acgatccca 
      gagtcggta gattccgaa atggaaccc atctattct 
      tgcccatac gaagccgac gaacaaagc atggtggaa 
      caatggctg aaactgcaa gaccaagga tggaacccg 
      caaaataaa gacgcaaca cccaaagat aataagaaa 
      caaaggtgc aagtcatcg aagagcaac agcagtggt 
      gaggaacat gaaaatagc tccagtgct tataacacg 
      ggaggatct tgtaacagt aatccttta actttcgag 
      ctagcctgt agccaggat tcgaaacaa aaggatacc 
      tatagatcc acgttaatt ttgtgtccc cccgtggag 
      aaggaagac acctcgaag gaaaaagac cagacggaa 
      acgtgcgat gcgtgcaaa cagcaagca acctccaaa 
      aagaaatct aagtcaacg tcaaaaacg aatagcccg 
      tcttctccc acgactaga ataggagtt ggagcaact 
      ccagcgcat ctgctctct gacaccatg tacacaaac 
      gtagctaat ttgcaacaa actatgctc ctgcagcag 
      caactgttt agacaagct ttagttcaa aataatatt 
      gacatagcg tcgaaacct acgacaagc ttcacatcc 
      ccgagctta agtcagtac caattcgtc agtagctca 
      cagacttac accacccaa cccgagact gccacttta 
      gaaacaatg atggaatgg aaggtcaag cgcagacct 
      gacggtacc aggtatata gcgcggcgg ccggttcgg 
      aataggata cttaaaaat agagcgatc aaaatatcg 
      gaagaaagg gcgggatta acgacggaa gacgacact 
      gtgtcggaa ctcaaaatt gggcggtac tggacgaag 
      gaggagcgc aaaaaacat ttagaaaag tcgaaggag 
      aggaagcag aggcaggag atattgcta gcgtcgagg 
      aacatcgag gaaaacggc gaattagtt atacataaa 
      ccggtcgag aaagttcac tcgttttcg tcgaagaaa 
      gccgcgaac aatttagat aatactgtg aaaaaacat 
      aaaagcaaa cgggcccac aaagaagga tatgataat 
      tttccaaca gtgcaagaa atgattgtc cacggaccc 
      aaagtcccc accaacggc aagatgatg ggactgctc 
      tccgtgaca acggtttaa tttaactcg ttattgaag 
      cttgctttt atatccact tattgcttt acttaggca 
      attttacgc taattccaa tttttctaa tatttacca 
      aatacatga gtgaatcaa ttcgatgat tttatgtga 
      gaaatttgt tgcctatag tatcccatt gtgatgttt 
      ctaatttag tgtacatat cagataaat tacaacaaa 
      tatatttca taactatta cctatttag atgtctaga 
      atgtttaaa tacgtgtgt gtaaataaa tattattat 
      aacca
    • >TC014168 RB
      acttctcgg agaagacat tgtgacctt ctgtgaccc 
      acttaattc ggacaaatt taaactgta gtttttgtc 
      agtgttacc tcccaagaa cggtacgta cattatttt 
      tcacacttc acatcaata aatcaacac caaacaact 
      ccagtctat tcaagtgag cagatgtct tcgactagt 
      taaacaaat ataatcaac tcgaaattt tatatacac 
      catccgaaa taaatcaaa aaaattctt ctagcgtaa 
      ctatagatt cgtttcccg aaatgttta tctaactcg 
      ctctcgtcc cgatattct ttacggaag gagtaaaaa 
      gtatagtgc aaaaacact ctcctagaa agtttttgg 
      tctgaattc agccgggct tagtctatt tattataat 
      tcaaagcat gttgtgact gtcggtcga tatgacaaa 
      gaaagaatg caacaaaaa ttcattcat cattcttta 
      atcatgcaa agtatagaa cagaccata atacgcgaa 
      aaccaacga tttgagcga aaatatgtt cttctaatt 
      ccgccattt ccatggtgg ttatattga gaatcgctt 
      agcaaggca actaattaa acatcacag tgggaacaa 
      ttgaatttt aagtcgatt tagacgtat aattacttt 
      tactgtcat aaccatcct gagcgtcca tcgtttcga 
      ttaagccct gacaattaa cagctatca agggtattt 
      accattcgg tttcgataa gcaatactt aggtattaa 
      gtggtcatc aggatgctg tgacgttac catttcaga 
      aactttgta ctgaatcca gattttttg tcaaagtgg 
      tgcagattg cagtaatta tcactgtgt attacacca 
      caaagacaa cttgcaacc gctaccaaa tctttaaag 
      tggaaaatg gcattacaa agtggcgtg gaaatataa 
      tcaactgaa gtgagatat acggtatct aattaatcg 
      caagccatg tagaacagc atgttatgc aatttccgg 
      aaaaaatgt ggatctgaa gtgcacttg gtggacacc 
      cacatcgtc cagaatcca tcagatttg acgttttta 
      tgtgttcaa acacttagc ccaataaat taatttcgg 
      gatctgggt ccgaagtgc aagttgtcc aggtgcccg 
      ccggcaata gtccggttg ttgttggtt ttattggcg 
      tgttgttga tttcggagt tccctcgga agtgaaggg 
      tctgagtta ttgttgtgc ttgtgccgg tggtcccgg 
      tgtcgaacg tttgaaatt gggtgtgtt gatatgttg 
      attggtggt gaggcattg tgtgcgtta attgcttct 
      gaggtccgg gcgcgggtc cgaaagagt cctaattga 
      attaaacgt gtcgcggca tccggaatg cgaccgggc 
      agcccctga accttgcca tagctatca acaatataa 
      gataatgaa cgcgtattc ctgatgaag agcaggggg 
      gcgctgggg tgcagactg cagcacaag tacagcacg 
      gagtcgcaa ccacggcgt tactcagtt ggaattcgt 
      gcctcagct ttggtggct gcagccgtt cagtggcct 
      cagtgctcg caatgcgct cgctctcca cggacgact 
      ctgcggact ccaacacac gcgttgtct cggtgcctc 
      tcgttccga tgcgatcga cttgtgctt gcaattgaa 
      cgcaacata catggattt tatcatact caaggtcaa 
      tgggactga cgtaagcag cttcccaca cgaagatgc 
      agttagagc atttctcac cgcacaaga acctattgt 
      tgttgaagt taagagaag aatatcgga gtcgccaca 
      agaaaaaac tcccacttc taaactgat ctcaacagg 
      ttgccaaac agaactttc gggattgac atggctgga 
      agataactc tttggactg tttgacaca cgatataga 
      cttggaggt gggtgcgtt ttcgatcga ctacgagtg 
      agggccggt agttagtgt tggaacaca taattattt 
      gagtgtgtt gtaacgtag gacgtaaac tttatggtg 
      gattttatt acatttaaa aggtcattc gtctttaag 
      ctgtaacta atttcgttt ctgtttgta aataaaatg 
      tccatcttc atcatcgtt gaagtgacg ttacgaaaa 
      tgtagtagc gacgagaag ttaggttta acagtttgc 
      tacagctcc ggttctgaa gctgacact tgcacggaa 
      gtgtatatt agagatata gcgccccaa agtgttgcc 
      gacagggat ggcagatta cgacaaggc gatcaaatt 
      ttacaagtc aatggaaaa gacgtcgct aataaagaa 
      gaaactgaa tccctcttc gctgaaaat agaaaagcc 
      gtcacatta ttggttagc agatgctat aacaggagc 
      gaagaatac ttggatgcc gaaccgatc gacgctatt 
      tcaccagga ggtcgcagt gccgcctac caaaacagt 
      cttatagaa caattagta caacagcag acagaatca 
      cagcattcc cctagaaac gacacagcc cccaaagtt 
      cctcctcac ttcatccaa gaccgactt aatctcacc 
      aatagttta gtacgttcg aaaatggac tcaataaat 
      cacgaaatt tccgagtta gaccaccgc atgcaaaac 
      atccagtta gtaaaaatc gacaagcgg aaacctccg 
      cagttacca cacatccca gcacctcaa gaatccgac 
      acggaacac atatatgaa acgatccca gagtcggta 
      gattccgaa atggaaccc atctattct tgcccatac 
      gaagccgac gaacaaagc atggtggaa caatggctg 
      aaactgcaa gaccaagga tggaacccg caaaataaa 
      gacgcaaca cccaaagat aataagaaa caaaggtgc 
      aagtcatcg aagagcaac agcagtggt gaggaacat 
      gaaaatagc tccagtgct tataacacg ggaggatct 
      tgtaacagt aatccttta actttcgag ctagcctgt 
      agccaggat tcgaaacaa aaggatacc tatagatcc 
      acgttaatt ttgtgtccc cccgtggag aaggaagac 
      acctcgaag gaaaaagac cagacggaa acgtgcgat 
      gcgtgcaaa cagcaagca acctccaaa aagaaatct 
      aagtcaacg tcaaaaacg aatagcccg tcttctccc 
      acgactaga ataggagtt ggagcaact ccagcgcat 
      ctgctctct gacaccatg tacacaaac gtagctaat 
      ttgcaacaa actatgctc ctgcagcag caactgttt 
      agacaagct ttagttcaa aataatatt gacatagcg 
      tcgaaacct acgacaagc ttcacatcc ccgagctta 
      agtcagtac caattcgtc agtagctca cagacttac 
      accacccaa cccgagact gccacttta gaaacaatg 
      atggaatgg aaggtcaag cgcagacct gacggtacc 
      aggtatata gcgcggcgg ccggttcgg aataggata 
      cttaaaaat agagcgatc aaaatatcg gaagaaagg 
      gcgggatta acgacggaa gacgacact gtgtcggaa 
      ctcaaaatt gggcggtac tggacgaag gaggagcgc 
      aaaaaacat ttagaaaag tcgaaggag aggaagcag 
      aggcaggag atattgcta gcgtcgagg aacatcgag 
      gaaaacggc gaattagtt atacataaa ccggtcgag 
      aaagttcac tcgttttcg tcgaagaaa gccgcgaac 
      aatttagat aatactgtg aaaaaacat aaaagcaaa 
      cgggcccac aaagaagga tatgataat tttccaaca 
      gtgcaagaa atgattgtc cacggaccc aaagtcccc 
      accaacggc aagatgatg ggactgctc tccgtgaca 
      acggtttaa tttaactcg ttattgaag cttgctttt 
      atatccact tattgcttt acttaggca attttacgc 
      taattccaa tttttctaa tatttacca aatacatga 
      gtgaatcaa ttcgatgat tttatgtga gaaatttgt 
      tgcctatag tatcccatt gtgatgttt ctaatttag 
      tgtacatat cagataaat tacaacaaa tatatttca 
      taactatta cctatttag atgtctaga atgtttaaa 
      tacgtgtgt gtaaataaa tattattat aacca