Status
Please wait ...
Gene information
General information
TC014163 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7086040..7089054
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014163
    iBeetle screen iB_02255

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014163 RA
      MEEEQDKGA AAAALDPAV LAINEELGA VAAAADQGA 
      VPAANSKRR HRKKDSKSW DNISKVITG MDEDSKVAY 
      IGEKYKDLH EQVRSLTAS NQQYVKYNA AIQKERDHH 
      QSELAKSVV ARARLENLC RELQKQNKQ IKEENLVKI 
      KEEEERRKE VSSKFADKL SDINNMMDE NKEKSEKLR 
      EENLRMTAR LADLFKQFK KREEDITRM SQQLELERQ 
      FAQATITKM ELEMRAQNE LNAKEQEVM KCNLEKSEA 
      NCSVLLQTV KGLQDHIEV YKNQYQDFE STMTKSANV 
      FDNFKAEIA TMNKQIATL EKETQFWKN KFNDSMKAL 
      IEVSELKKN QDLYVQSLE RKVEQLNKL CRQIQVDRS 
      HYLKLLKNS GIELTPPSQ PQEKTEEAK KASPTMTKK 
      EQELHLLKE NLKVVQDQL ARLSVEGTQ KTEEHNCEG 
      PACKICDPT TVPASDGDF SQVPLEVQK EENHAPGST 
      EESQ
    CDS sequence
    • >TC014163 RA
      atggaggaa gaacaggat aagggggct gcagcggcg 
      gccttggac ccagcggtt ctagcaatc aacgaggaa 
      ttaggggct gtagctgcg gccgccgat cagggggct 
      gttccagcg gctaattcc aagagaaga catcgcaag 
      aaggactcc aaatcgtgg gacaatatc tcaaaagta 
      atcaccggg atggacgaa gacagcaaa gtcgcctac 
      attggagag aaatacaaa gacttgcat gagcaagtg 
      cgatctttg acggccagt aaccagcaa tacgtgaaa 
      tacaacgca gctatccag aaggagaga gatcatcac 
      cagtccgaa ttggccaag agtgtcgta gctagggct 
      cggctggag aatctgtgc cgggagctg caaaagcaa 
      aacaagcag atcaaggag gagaatttg gtcaagata 
      aaggaagag gaagagcgg cgcaaggag gtttcctcg 
      aagtttgcc gataagttg agcgatatc aacaacatg 
      atggatgag aacaaggag aagagtgaa aaactgcgc 
      gaggagaat ctccgaatg accgctaga ttagctgat 
      ttatttaaa caattcaag aaacgtgag gaggatatc 
      acaaggatg tcgcagcag cttgagttg gagcgtcag 
      tttgctcaa gccactata actaaaatg gagttggaa 
      atgagggct caaaacgaa ctgaatgcc aaagaacaa 
      gaagtcatg aaatgcaac cttgaaaaa agcgaagct 
      aattgttcc gtcctgttg cagacagtt aagggcctc 
      caagaccac attgaagtg tacaaaaac cagtatcaa 
      gattttgag tcgaccatg accaagagt gcaaacgtt 
      tttgataat ttcaaagca gaaattgcc accatgaat 
      aaacaaata gccactttg gaaaaggag actcaattt 
      tggaaaaat aaatttaac gatagcatg aaagccttg 
      attgaagtg agcgagttg aagaaaaat caggatttg 
      tacgttcaa agtcttgaa cggaaagtc gaacaattg 
      aataaactg tgccgccaa atacaagtc gatcgtagt 
      cactatttg aagctgttg aagaactcg ggaattgaa 
      ctgactccc cctagtcaa ccgcaagaa aagactgaa 
      gaggctaaa aaagccagt ccaacaatg accaaaaaa 
      gagcaagaa ttacacttg cttaaggaa aacttgaag 
      gtcgttcaa gatcagtta gcaagactg agtgttgaa 
      ggaacccag aagactgag gagcataat tgcgagggt 
      ccagcgtgc aaaatatgc gaccccaca actgtacct 
      gcatcggac ggtgatttt tcgcaagtt cccttggaa 
      gtccaaaag gaagaaaat cacgcccca gggtctaca 
      gaggaaagt cagtaa
    mRNA sequence
    • >TC014163 RA
      ttttaaaac aaagctctg ttggaaaaa gttcgtggc 
      tcttgtaga ctgtgcttc gctttgata aaaacttgt 
      tgtttgcgc tatcgattg ataaattgt taagttgtt 
      tacaagaag ccggctatg gaggaagaa caggataag 
      ggggctgca gcggcggcc ttggaccca gcggttcta 
      gcaatcaac gaggaatta ggggctgta gctgcggcc 
      gccgatcag ggggctgtt ccagcggct aattccaag 
      agaagacat cgcaagaag gactccaaa tcgtgggac 
      aatatctca aaagtaatc accgggatg gacgaagac 
      agcaaagtc gcctacatt ggagagaaa tacaaagac 
      ttgcatgag caagtgcga tctttgacg gccagtaac 
      cagcaatac gtgaaatac aacgcagct atccagaag 
      gagagagat catcaccag tccgaattg gccaagagt 
      gtcgtagct agggctcgg ctggagaat ctgtgccgg 
      gagctgcaa aagcaaaac aagcagatc aaggaggag 
      aatttggtc aagataaag gaagaggaa gagcggcgc 
      aaggaggtt tcctcgaag tttgccgat aagttgagc 
      gatatcaac aacatgatg gatgagaac aaggagaag 
      agtgaaaaa ctgcgcgag gagaatctc cgaatgacc 
      gctagatta gctgattta tttaaacaa ttcaagaaa 
      cgtgaggag gatatcaca aggatgtcg cagcagctt 
      gagttggag cgtcagttt gctcaagcc actataact 
      aaaatggag ttggaaatg agggctcaa aacgaactg 
      aatgccaaa gaacaagaa gtcatgaaa tgcaacctt 
      gaaaaaagc gaagctaat tgttccgtc ctgttgcag 
      acagttaag ggcctccaa gaccacatt gaagtgtac 
      aaaaaccag tatcaagat tttgagtcg accatgacc 
      aagagtgca aacgttttt gataatttc aaagcagaa 
      attgccacc atgaataaa caaatagcc actttggaa 
      aaggagact caattttgg aaaaataaa tttaacgat 
      agcatgaaa gccttgatt gaagtgagc gagttgaag 
      aaaaatcag gatttgtac gttcaaagt cttgaacgg 
      aaagtcgaa caattgaat aaactgtgc cgccaaata 
      caagtcgat cgtagtcac tatttgaag ctgttgaag 
      aactcggga attgaactg actccccct agtcaaccg 
      caagaaaag actgaagag gctaaaaaa gccagtcca 
      acaatgacc aaaaaagag caagaatta cacttgctt 
      aaggaaaac ttgaaggtc gttcaagat cagttagca 
      agactgagt gttgaagga acccagaag actgaggag 
      cataattgc gagggtcca gcgtgcaaa atatgcgac 
      cccacaact gtacctgca tcggacggt gatttttcg 
      caagttccc ttggaagtc caaaaggaa gaaaatcac 
      gccccaggg tctacagag gaaagtcag taaggcgtt 
      ccaagtgac tcatgtgat caacttgcc ccagtggtt 
      ttagaataa ttatttatt tttcgtttt tttaagtac 
      ttaaacaat atattttca tttttttgc atgttttca 
      ttcaagtat tatctaaag gaagcacag tgataatac 
      tcgattttt gtgattttt tttagatag gtgttttgt 
      tttgtaacg ttacctgtc ttaaattta tatttgttt 
      cagagtttc tattcagtt agtttagtg ttctgtttt 
      attgttcag aaaaaattt ttagtttaa tttttcaaa 
      tactactat taacgtgtt tttgtaact tgtttaatt 
      acagtgcct ttaactttg ttctgtgaa ttacctaat 
      aaaattttt caattaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY