Status
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Gene information
General information
TC014127 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6249171..6269071
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014127
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02253

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Many hatched, dead animals with diffuse green fluorescence. They presumably died directly after hatching. Most unhatched larvae show normal muscle fluorescence.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014127 RA
      MKQSVYYFD IVDYIIFGA MLFLSALTG IYFGCRGKI 
      NKKREPESF KDYLTGNKN LKPFPVAMS LIASYISGV 
      TMLGTPAEV YNFGAQYWL IVFAMSLSG LTVATVYLP 
      VFTKLQVCS SYEYLELRF SKILRTIAS LLFLLDEIM 
      FLPMLIYVP SLAFNQVSG ANLHLIGAI VCTVCVFYT 
      ILGGLRAVV YTDTWQTVV MFISDMVVV ILGVVAIGG 
      MSVIIERSS AGNRIQSLN FNPSMYERY TVFSVLIGG 
      FTYWTSFNA VNQTMVQRY LSLPNNKEA RIAIAIFTV 
      GVVFFISMC CFAGLLLFG TYHDCDPLA ANRISNSDQ 
      LLPLFVVET AGHLRGVPG LFIAGVFGA ALSSLSVVL 
      NSSAVVLLE DFFRGVCHF SITERQGKL FTKSIILIL 
      GIASVCLVF VVEKMGSVF AVATSLSAI VAGTMFGVF 
      TLGMLVPWA NVKGAVVGS VSGFLMSCI VSYGQMYVA 
      SAKMVVPRP LPVSIEGCA ATYGLNVTE PPEIEYPDE 
      SKVFPLFRL SFLWITPIG VFTVIAVGI ITSFLTGKT 
      DLKTLDPEL ISPVMHWVL PEEAQNYAG SAVRKIKHQ 
      RLFGNEAIA SPSVTMNLN VLLSYGIIF VT
    • >TC014127 RB
      MKQSVYYFD IVDYIIFGA MLFLSALTG IYFGCRGKI 
      NKKREPESF KDYLTGNKN LKPFPVAMS LIASYISGV 
      TMLGTPAEV YNFGAQYWL IVFAMSLSG LTVATVYLP 
      VFTKLQVCS SYEYLELRF SKILRTIAS LLFLLDEIM 
      FLPMLIYVP SLAFNQVSG ANLHLIGAI VCTVCVFYT 
      ILGGLRAVV YTDTWQTVV MFISDMVVV ILGVVAIGG 
      MSVIIERSS AGNRIQSLN FNPSMYERY TVFSVLIGG 
      FTYWTSFNA VNQTMVQRY LSLPNNKEA RIAIAIFTV 
      GVVFFISMC CFAGLLLFG TYHDCDPLA ANRISNSDQ 
      LLPLFVVET AGHLRGVPG LFIAGVFGA ALSSLSVVL 
      NSSAVVLLE DFFRGVCHF SITERQGKL FTKSIILIL 
      GIASVCLVF VVEKMGSVF AVATSLSAI VAGTMFGVF 
      TLGMLVPWA NVKGAVVGS VSGFLMSCI VSYGQMYVA 
      SAKMVVPRP LPVSIEGCA ATYGLNVTE PPEIEYPDE 
      SKVFPLFRL SFLWITPIG VFTVIAVGI ITSFLTGKT 
      DLKTLDPEL ISPVMHWVL PEEAQNYAG SAVRKIKHQ 
      RLFGNEAIA SPSVTMNLN VLLSYGDHE EGRTDTSAG 
      GQSNPTDLS PVFIEPNLL TYSTWNWNS TGNVTRRVV 
      LDGSTVDAT RPHLAPGER SGTDDGRRA TSASTARRD 
      DRRFSCFQE REGQVAT
    CDS sequence
    • >TC014127 RA
      atgaagcaa tccgtttat tatttcgat attgtcgat 
      tatattatt tttggggcc atgttattt ttgtcggcc 
      ctaactggt atttatttt ggctgtaga ggaaaaatc 
      aacaaaaag cgggaaccg gaatctttt aaagattat 
      ctaacagga aacaaaaat cttaaacct tttccggtt 
      gccatgtcg ctaattgca agttacata tcaggggtg 
      acaatgctt ggaaccccc gccgaagtg tacaatttt 
      ggagctcag tattggtta attgttttt gcaatgtcc 
      ctcagtggt ctcacagtg gctaccgtt tatctacca 
      gtattcacc aagctgcaa gtttgttcc tcatacgag 
      tatttggag ctaagattc agcaagatc ctgcgaaca 
      atagcgtcc cttttattc ctcttagac gaaatcatg 
      tttttacca atgttgatc tacgttccg tcccttgca 
      tttaatcaa gtctctggg gcaaatcta catttgatc 
      ggtgctatt gtctgcacg gtttgcgtt ttttacaca 
      attttgggt ggtttgagg gctgtggtt tacaccgac 
      acttggcaa actgtagtt atgtttatt tccgacatg 
      gtagtggta attttggga gtcgttgcc atcggagga 
      atgagtgtt attatcgaa cgatcctct gctggtaac 
      aggatccag tcactgaat tttaatccc agtatgtac 
      gaaagatat acagtattt agcgttcta attggagga 
      ttcacttac tggacttct ttcaatgcg gtcaatcag 
      acaatggtg cagagatac ttatcatta ccaaataac 
      aaagaggct agaatagcc atagctatt tttacagta 
      ggagttgta ttttttata tcaatgtgt tgtttcgct 
      ggtcttctt ctatttggc acatatcat gactgtgat 
      cctctagca gcaaacaga atatcaaac agtgaccag 
      cttcttcct ctgtttgtt gttgaaact gcgggacat 
      ttaagaggc gttcctggg ctttttatt gctggtgtt 
      tttggagct gcattaagt tctttatcg gttgttcta 
      aactcatca gctgttgtt ttgttggaa gactttttc 
      cgcggagtt tgtcatttt agtataacg gagagacag 
      ggcaaacta tttacgaaa agtataata ttaattcta 
      ggaattgct tctgtatgt cttgttttt gttgttgaa 
      aaaatgggt agcgtcttt gcagttgcc acttcgcta 
      tctgcaatt gttgcagga acaatgttt ggtgttttt 
      acgcttgga atgctagta ccttgggct aacgtcaag 
      ggcgcagtg gtgggctca gttagcgga tttttaatg 
      tcttgtatc gtttcttac ggtcaaatg tatgtcgct 
      tctgctaaa atggtggtc cctcggcca cttcctgtg 
      tctatagaa ggctgcgct gcaacttat ggtctcaat 
      gttaccgag ccgcctgaa atagaatat ccggatgaa 
      agcaaggtt tttcctctg ttccggttg tctttcctg 
      tggataaca ccgattggt gttttcacg gtgatcgct 
      gtgggtatt ataacgagt tttctgacg ggaaaaact 
      gatctgaag actttggat ccggagctg atatcgcct 
      gtaatgcac tgggtcctg cccgaggag gcgcagaat 
      tacgcagga tctgctgtg aggaagatt aaacaccag 
      cgactgttt ggcaatgag gcaattgca tcgccttca 
      gtaacaatg aatttaaat gtattactt tcatacggt 
      attattttc gttacttaa
    • >TC014127 RB
      atgaagcaa tccgtttat tatttcgat attgtcgat 
      tatattatt tttggggcc atgttattt ttgtcggcc 
      ctaactggt atttatttt ggctgtaga ggaaaaatc 
      aacaaaaag cgggaaccg gaatctttt aaagattat 
      ctaacagga aacaaaaat cttaaacct tttccggtt 
      gccatgtcg ctaattgca agttacata tcaggggtg 
      acaatgctt ggaaccccc gccgaagtg tacaatttt 
      ggagctcag tattggtta attgttttt gcaatgtcc 
      ctcagtggt ctcacagtg gctaccgtt tatctacca 
      gtattcacc aagctgcaa gtttgttcc tcatacgag 
      tatttggag ctaagattc agcaagatc ctgcgaaca 
      atagcgtcc cttttattc ctcttagac gaaatcatg 
      tttttacca atgttgatc tacgttccg tcccttgca 
      tttaatcaa gtctctggg gcaaatcta catttgatc 
      ggtgctatt gtctgcacg gtttgcgtt ttttacaca 
      attttgggt ggtttgagg gctgtggtt tacaccgac 
      acttggcaa actgtagtt atgtttatt tccgacatg 
      gtagtggta attttggga gtcgttgcc atcggagga 
      atgagtgtt attatcgaa cgatcctct gctggtaac 
      aggatccag tcactgaat tttaatccc agtatgtac 
      gaaagatat acagtattt agcgttcta attggagga 
      ttcacttac tggacttct ttcaatgcg gtcaatcag 
      acaatggtg cagagatac ttatcatta ccaaataac 
      aaagaggct agaatagcc atagctatt tttacagta 
      ggagttgta ttttttata tcaatgtgt tgtttcgct 
      ggtcttctt ctatttggc acatatcat gactgtgat 
      cctctagca gcaaacaga atatcaaac agtgaccag 
      cttcttcct ctgtttgtt gttgaaact gcgggacat 
      ttaagaggc gttcctggg ctttttatt gctggtgtt 
      tttggagct gcattaagt tctttatcg gttgttcta 
      aactcatca gctgttgtt ttgttggaa gactttttc 
      cgcggagtt tgtcatttt agtataacg gagagacag 
      ggcaaacta tttacgaaa agtataata ttaattcta 
      ggaattgct tctgtatgt cttgttttt gttgttgaa 
      aaaatgggt agcgtcttt gcagttgcc acttcgcta 
      tctgcaatt gttgcagga acaatgttt ggtgttttt 
      acgcttgga atgctagta ccttgggct aacgtcaag 
      ggcgcagtg gtgggctca gttagcgga tttttaatg 
      tcttgtatc gtttcttac ggtcaaatg tatgtcgct 
      tctgctaaa atggtggtc cctcggcca cttcctgtg 
      tctatagaa ggctgcgct gcaacttat ggtctcaat 
      gttaccgag ccgcctgaa atagaatat ccggatgaa 
      agcaaggtt tttcctctg ttccggttg tctttcctg 
      tggataaca ccgattggt gttttcacg gtgatcgct 
      gtgggtatt ataacgagt tttctgacg ggaaaaact 
      gatctgaag actttggat ccggagctg atatcgcct 
      gtaatgcac tgggtcctg cccgaggag gcgcagaat 
      tacgcagga tctgctgtg aggaagatt aaacaccag 
      cgactgttt ggcaatgag gcaattgca tcgccttca 
      gtaacaatg aatttaaat gtattactt tcatacggt 
      gaccacgaa gaaggccgc accgatacc tccgccggg 
      ggccaatca aaccccact gatttgtca cctgtcttc 
      atcgagccc aacctcctc acatactct acttggaat 
      tggaattct actggcaat gtcactcga agagtggtt 
      cttgacggt tccaccgtg gacgccaca cggccgcac 
      ttggcacca ggtgagcgt tcgggcacc gacgacggc 
      cgacgggcc acgagcgct tccacagcc cgcagagac 
      gaccgccgg ttcagttgc tttcaggag cgcgaaggg 
      caagttgct acttag
    mRNA sequence
    • >TC014127 RA
      ttaagtata aattaattt ttaggtatt aacaaccga 
      acaaattcc gagccactt aatcgacca gttgtttga 
      agacatcgt aacaaatca attcgttca aaatgaagc 
      aatccgttt attatttcg atattgtcg attatatta 
      tttttgggg ccatgttat ttttgtcgg ccctaactg 
      gtatttatt ttggctgta gaggaaaaa tcaacaaaa 
      agcgggaac cggaatctt ttaaagatt atctaacag 
      gaaacaaaa atcttaaac cttttccgg ttgccatgt 
      cgctaattg caagttaca tatcagggg tgacaatgc 
      ttggaaccc ccgccgaag tgtacaatt ttggagctc 
      agtattggt taattgttt ttgcaatgt ccctcagtg 
      gtctcacag tggctaccg tttatctac cagtattca 
      ccaagctgc aagtttgtt cctcatacg agtatttgg 
      agctaagat tcagcaaga tcctgcgaa caatagcgt 
      cccttttat tcctcttag acgaaatca tgtttttac 
      caatgttga tctacgttc cgtcccttg catttaatc 
      aagtctctg gggcaaatc tacatttga tcggtgcta 
      ttgtctgca cggtttgcg ttttttaca caattttgg 
      gtggtttga gggctgtgg tttacaccg acacttggc 
      aaactgtag ttatgttta tttccgaca tggtagtgg 
      taattttgg gagtcgttg ccatcggag gaatgagtg 
      ttattatcg aacgatcct ctgctggta acaggatcc 
      agtcactga attttaatc ccagtatgt acgaaagat 
      atacagtat ttagcgttc taattggag gattcactt 
      actggactt ctttcaatg cggtcaatc agacaatgg 
      tgcagagat acttatcat taccaaata acaaagagg 
      ctagaatag ccatagcta tttttacag taggagttg 
      tatttttta tatcaatgt gttgtttcg ctggtcttc 
      ttctatttg gcacatatc atgactgtg atcctctag 
      cagcaaaca gaatatcaa acagtgacc agcttcttc 
      ctctgtttg ttgttgaaa ctgcgggac atttaagag 
      gcgttcctg ggcttttta ttgctggtg tttttggag 
      ctgcattaa gttctttat cggttgttc taaactcat 
      cagctgttg ttttgttgg aagactttt tccgcggag 
      tttgtcatt ttagtataa cggagagac agggcaaac 
      tatttacga aaagtataa tattaattc taggaattg 
      cttctgtat gtcttgttt ttgttgttg aaaaaatgg 
      gtagcgtct ttgcagttg ccacttcgc tatctgcaa 
      ttgttgcag gaacaatgt ttggtgttt ttacgcttg 
      gaatgctag taccttggg ctaacgtca agggcgcag 
      tggtgggct cagttagcg gatttttaa tgtcttgta 
      tcgtttctt acggtcaaa tgtatgtcg cttctgcta 
      aaatggtgg tccctcggc cacttcctg tgtctatag 
      aaggctgcg ctgcaactt atggtctca atgttaccg 
      agccgcctg aaatagaat atccggatg aaagcaagg 
      tttttcctc tgttccggt tgtctttcc tgtggataa 
      caccgattg gtgttttca cggtgatcg ctgtgggta 
      ttataacga gttttctga cgggaaaaa ctgatctga 
      agactttgg atccggagc tgatatcgc ctgtaatgc 
      actgggtcc tgcccgagg aggcgcaga attacgcag 
      gatctgctg tgaggaaga ttaaacacc agcgactgt 
      ttggcaatg aggcaattg catcgcctt cagtaacaa 
      tgaatttaa atgtattac tttcatacg gtattattt 
      tcgttactt aatactttt ttacaggag agacgagat 
      acggaaagt cccgacaac agatgaaaa ttgaaaaca 
      tgttataac ttatctcac ttttgtata aatgttgta 
      ctcattaat attgatgtt ttattaata aaatttaaa 
      cgtaaag
    • >TC014127 RB
      aaggttttg ttgccaatt atttctcgt gttgggagc 
      gaccacgtt gtctattta tttataaaa cgttacatg 
      gactgtatt aacaaccga acaaattcc gagccactt 
      aatcgacca gttgtttga agacatcgt aacaaatca 
      attcgttca aaatgaagc aatccgttt attatttcg 
      atattgtcg attatatta tttttgggg ccatgttat 
      ttttgtcgg ccctaactg gtatttatt ttggctgta 
      gaggaaaaa tcaacaaaa agcgggaac cggaatctt 
      ttaaagatt atctaacag gaaacaaaa atcttaaac 
      cttttccgg ttgccatgt cgctaattg caagttaca 
      tatcagggg tgacaatgc ttggaaccc ccgccgaag 
      tgtacaatt ttggagctc agtattggt taattgttt 
      ttgcaatgt ccctcagtg gtctcacag tggctaccg 
      tttatctac cagtattca ccaagctgc aagtttgtt 
      cctcatacg agtatttgg agctaagat tcagcaaga 
      tcctgcgaa caatagcgt cccttttat tcctcttag 
      acgaaatca tgtttttac caatgttga tctacgttc 
      cgtcccttg catttaatc aagtctctg gggcaaatc 
      tacatttga tcggtgcta ttgtctgca cggtttgcg 
      ttttttaca caattttgg gtggtttga gggctgtgg 
      tttacaccg acacttggc aaactgtag ttatgttta 
      tttccgaca tggtagtgg taattttgg gagtcgttg 
      ccatcggag gaatgagtg ttattatcg aacgatcct 
      ctgctggta acaggatcc agtcactga attttaatc 
      ccagtatgt acgaaagat atacagtat ttagcgttc 
      taattggag gattcactt actggactt ctttcaatg 
      cggtcaatc agacaatgg tgcagagat acttatcat 
      taccaaata acaaagagg ctagaatag ccatagcta 
      tttttacag taggagttg tatttttta tatcaatgt 
      gttgtttcg ctggtcttc ttctatttg gcacatatc 
      atgactgtg atcctctag cagcaaaca gaatatcaa 
      acagtgacc agcttcttc ctctgtttg ttgttgaaa 
      ctgcgggac atttaagag gcgttcctg ggcttttta 
      ttgctggtg tttttggag ctgcattaa gttctttat 
      cggttgttc taaactcat cagctgttg ttttgttgg 
      aagactttt tccgcggag tttgtcatt ttagtataa 
      cggagagac agggcaaac tatttacga aaagtataa 
      tattaattc taggaattg cttctgtat gtcttgttt 
      ttgttgttg aaaaaatgg gtagcgtct ttgcagttg 
      ccacttcgc tatctgcaa ttgttgcag gaacaatgt 
      ttggtgttt ttacgcttg gaatgctag taccttggg 
      ctaacgtca agggcgcag tggtgggct cagttagcg 
      gatttttaa tgtcttgta tcgtttctt acggtcaaa 
      tgtatgtcg cttctgcta aaatggtgg tccctcggc 
      cacttcctg tgtctatag aaggctgcg ctgcaactt 
      atggtctca atgttaccg agccgcctg aaatagaat 
      atccggatg aaagcaagg tttttcctc tgttccggt 
      tgtctttcc tgtggataa caccgattg gtgttttca 
      cggtgatcg ctgtgggta ttataacga gttttctga 
      cgggaaaaa ctgatctga agactttgg atccggagc 
      tgatatcgc ctgtaatgc actgggtcc tgcccgagg 
      aggcgcaga attacgcag gatctgctg tgaggaaga 
      ttaaacacc agcgactgt ttggcaatg aggcaattg 
      catcgcctt cagtaacaa tgaatttaa atgtattac 
      tttcatacg gtgaccacg aagaaggcc gcaccgata 
      cctccgccg ggggccaat caaacccca ctgatttgt 
      cacctgtct tcatcgagc ccaacctcc tcacatact 
      ctacttgga attggaatt ctactggca atgtcactc 
      gaagagtgg ttcttgacg gttccaccg tggacgcca 
      cacggccgc acttggcac caggtgagc gttcgggca 
      ccgacgacg gccgacggg ccacgagcg cttccacag 
      cccgcagag acgaccgcc ggttcagtt gctttcagg 
      agcgcgaag ggcaagttg ctacttaga gccctcgtc 
      gactattga ccgcgtgct ttaatcaaa tataaacaa 
      atattgctg ctttctaca cacatcgct tgtgattgt 
      gccggtttt gaccgcctg tgattgctt gtttgtggg 
      atatttcgt ggggtttgg gcacttctc tcgcccgcc 
      accaagcca aactagact gagtgccat ggtctgacg 
      acgaaacga ccgcacgat tcctacgat ttatttcgg 
      agagtattt tacgctgac tttgtcagt tcaacaaaa 
      gtaagtttg ttgttaatt ttacttatt tgttcaaat 
      tgtgcttaa atacttgtt caggagata aataaacga 
      aaagattaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY