| General information | |||
| TC014127 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 6249171..6269071 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014127 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02253 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
| Comment: Many hatched, dead animals with diffuse green fluorescence. They presumably died directly after hatching. Most unhatched larvae show normal muscle fluorescence. |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC014127 RA
MKQSVYYFD IVDYIIFGA MLFLSALTG IYFGCRGKI NKKREPESF KDYLTGNKN LKPFPVAMS LIASYISGV TMLGTPAEV YNFGAQYWL IVFAMSLSG LTVATVYLP VFTKLQVCS SYEYLELRF SKILRTIAS LLFLLDEIM FLPMLIYVP SLAFNQVSG ANLHLIGAI VCTVCVFYT ILGGLRAVV YTDTWQTVV MFISDMVVV ILGVVAIGG MSVIIERSS AGNRIQSLN FNPSMYERY TVFSVLIGG FTYWTSFNA VNQTMVQRY LSLPNNKEA RIAIAIFTV GVVFFISMC CFAGLLLFG TYHDCDPLA ANRISNSDQ LLPLFVVET AGHLRGVPG LFIAGVFGA ALSSLSVVL NSSAVVLLE DFFRGVCHF SITERQGKL FTKSIILIL GIASVCLVF VVEKMGSVF AVATSLSAI VAGTMFGVF TLGMLVPWA NVKGAVVGS VSGFLMSCI VSYGQMYVA SAKMVVPRP LPVSIEGCA ATYGLNVTE PPEIEYPDE SKVFPLFRL SFLWITPIG VFTVIAVGI ITSFLTGKT DLKTLDPEL ISPVMHWVL PEEAQNYAG SAVRKIKHQ RLFGNEAIA SPSVTMNLN VLLSYGIIF VT
- >TC014127 RB
MKQSVYYFD IVDYIIFGA MLFLSALTG IYFGCRGKI NKKREPESF KDYLTGNKN LKPFPVAMS LIASYISGV TMLGTPAEV YNFGAQYWL IVFAMSLSG LTVATVYLP VFTKLQVCS SYEYLELRF SKILRTIAS LLFLLDEIM FLPMLIYVP SLAFNQVSG ANLHLIGAI VCTVCVFYT ILGGLRAVV YTDTWQTVV MFISDMVVV ILGVVAIGG MSVIIERSS AGNRIQSLN FNPSMYERY TVFSVLIGG FTYWTSFNA VNQTMVQRY LSLPNNKEA RIAIAIFTV GVVFFISMC CFAGLLLFG TYHDCDPLA ANRISNSDQ LLPLFVVET AGHLRGVPG LFIAGVFGA ALSSLSVVL NSSAVVLLE DFFRGVCHF SITERQGKL FTKSIILIL GIASVCLVF VVEKMGSVF AVATSLSAI VAGTMFGVF TLGMLVPWA NVKGAVVGS VSGFLMSCI VSYGQMYVA SAKMVVPRP LPVSIEGCA ATYGLNVTE PPEIEYPDE SKVFPLFRL SFLWITPIG VFTVIAVGI ITSFLTGKT DLKTLDPEL ISPVMHWVL PEEAQNYAG SAVRKIKHQ RLFGNEAIA SPSVTMNLN VLLSYGDHE EGRTDTSAG GQSNPTDLS PVFIEPNLL TYSTWNWNS TGNVTRRVV LDGSTVDAT RPHLAPGER SGTDDGRRA TSASTARRD DRRFSCFQE REGQVAT
- >TC014127 RA
atgaagcaa tccgtttat tatttcgat attgtcgat tatattatt tttggggcc atgttattt ttgtcggcc ctaactggt atttatttt ggctgtaga ggaaaaatc aacaaaaag cgggaaccg gaatctttt aaagattat ctaacagga aacaaaaat cttaaacct tttccggtt gccatgtcg ctaattgca agttacata tcaggggtg acaatgctt ggaaccccc gccgaagtg tacaatttt ggagctcag tattggtta attgttttt gcaatgtcc ctcagtggt ctcacagtg gctaccgtt tatctacca gtattcacc aagctgcaa gtttgttcc tcatacgag tatttggag ctaagattc agcaagatc ctgcgaaca atagcgtcc cttttattc ctcttagac gaaatcatg tttttacca atgttgatc tacgttccg tcccttgca tttaatcaa gtctctggg gcaaatcta catttgatc ggtgctatt gtctgcacg gtttgcgtt ttttacaca attttgggt ggtttgagg gctgtggtt tacaccgac acttggcaa actgtagtt atgtttatt tccgacatg gtagtggta attttggga gtcgttgcc atcggagga atgagtgtt attatcgaa cgatcctct gctggtaac aggatccag tcactgaat tttaatccc agtatgtac gaaagatat acagtattt agcgttcta attggagga ttcacttac tggacttct ttcaatgcg gtcaatcag acaatggtg cagagatac ttatcatta ccaaataac aaagaggct agaatagcc atagctatt tttacagta ggagttgta ttttttata tcaatgtgt tgtttcgct ggtcttctt ctatttggc acatatcat gactgtgat cctctagca gcaaacaga atatcaaac agtgaccag cttcttcct ctgtttgtt gttgaaact gcgggacat ttaagaggc gttcctggg ctttttatt gctggtgtt tttggagct gcattaagt tctttatcg gttgttcta aactcatca gctgttgtt ttgttggaa gactttttc cgcggagtt tgtcatttt agtataacg gagagacag ggcaaacta tttacgaaa agtataata ttaattcta ggaattgct tctgtatgt cttgttttt gttgttgaa aaaatgggt agcgtcttt gcagttgcc acttcgcta tctgcaatt gttgcagga acaatgttt ggtgttttt acgcttgga atgctagta ccttgggct aacgtcaag ggcgcagtg gtgggctca gttagcgga tttttaatg tcttgtatc gtttcttac ggtcaaatg tatgtcgct tctgctaaa atggtggtc cctcggcca cttcctgtg tctatagaa ggctgcgct gcaacttat ggtctcaat gttaccgag ccgcctgaa atagaatat ccggatgaa agcaaggtt tttcctctg ttccggttg tctttcctg tggataaca ccgattggt gttttcacg gtgatcgct gtgggtatt ataacgagt tttctgacg ggaaaaact gatctgaag actttggat ccggagctg atatcgcct gtaatgcac tgggtcctg cccgaggag gcgcagaat tacgcagga tctgctgtg aggaagatt aaacaccag cgactgttt ggcaatgag gcaattgca tcgccttca gtaacaatg aatttaaat gtattactt tcatacggt attattttc gttacttaa
- >TC014127 RB
atgaagcaa tccgtttat tatttcgat attgtcgat tatattatt tttggggcc atgttattt ttgtcggcc ctaactggt atttatttt ggctgtaga ggaaaaatc aacaaaaag cgggaaccg gaatctttt aaagattat ctaacagga aacaaaaat cttaaacct tttccggtt gccatgtcg ctaattgca agttacata tcaggggtg acaatgctt ggaaccccc gccgaagtg tacaatttt ggagctcag tattggtta attgttttt gcaatgtcc ctcagtggt ctcacagtg gctaccgtt tatctacca gtattcacc aagctgcaa gtttgttcc tcatacgag tatttggag ctaagattc agcaagatc ctgcgaaca atagcgtcc cttttattc ctcttagac gaaatcatg tttttacca atgttgatc tacgttccg tcccttgca tttaatcaa gtctctggg gcaaatcta catttgatc ggtgctatt gtctgcacg gtttgcgtt ttttacaca attttgggt ggtttgagg gctgtggtt tacaccgac acttggcaa actgtagtt atgtttatt tccgacatg gtagtggta attttggga gtcgttgcc atcggagga atgagtgtt attatcgaa cgatcctct gctggtaac aggatccag tcactgaat tttaatccc agtatgtac gaaagatat acagtattt agcgttcta attggagga ttcacttac tggacttct ttcaatgcg gtcaatcag acaatggtg cagagatac ttatcatta ccaaataac aaagaggct agaatagcc atagctatt tttacagta ggagttgta ttttttata tcaatgtgt tgtttcgct ggtcttctt ctatttggc acatatcat gactgtgat cctctagca gcaaacaga atatcaaac agtgaccag cttcttcct ctgtttgtt gttgaaact gcgggacat ttaagaggc gttcctggg ctttttatt gctggtgtt tttggagct gcattaagt tctttatcg gttgttcta aactcatca gctgttgtt ttgttggaa gactttttc cgcggagtt tgtcatttt agtataacg gagagacag ggcaaacta tttacgaaa agtataata ttaattcta ggaattgct tctgtatgt cttgttttt gttgttgaa aaaatgggt agcgtcttt gcagttgcc acttcgcta tctgcaatt gttgcagga acaatgttt ggtgttttt acgcttgga atgctagta ccttgggct aacgtcaag ggcgcagtg gtgggctca gttagcgga tttttaatg tcttgtatc gtttcttac ggtcaaatg tatgtcgct tctgctaaa atggtggtc cctcggcca cttcctgtg tctatagaa ggctgcgct gcaacttat ggtctcaat gttaccgag ccgcctgaa atagaatat ccggatgaa agcaaggtt tttcctctg ttccggttg tctttcctg tggataaca ccgattggt gttttcacg gtgatcgct gtgggtatt ataacgagt tttctgacg ggaaaaact gatctgaag actttggat ccggagctg atatcgcct gtaatgcac tgggtcctg cccgaggag gcgcagaat tacgcagga tctgctgtg aggaagatt aaacaccag cgactgttt ggcaatgag gcaattgca tcgccttca gtaacaatg aatttaaat gtattactt tcatacggt gaccacgaa gaaggccgc accgatacc tccgccggg ggccaatca aaccccact gatttgtca cctgtcttc atcgagccc aacctcctc acatactct acttggaat tggaattct actggcaat gtcactcga agagtggtt cttgacggt tccaccgtg gacgccaca cggccgcac ttggcacca ggtgagcgt tcgggcacc gacgacggc cgacgggcc acgagcgct tccacagcc cgcagagac gaccgccgg ttcagttgc tttcaggag cgcgaaggg caagttgct acttag
- >TC014127 RA
ttaagtata aattaattt ttaggtatt aacaaccga acaaattcc gagccactt aatcgacca gttgtttga agacatcgt aacaaatca attcgttca aaatgaagc aatccgttt attatttcg atattgtcg attatatta tttttgggg ccatgttat ttttgtcgg ccctaactg gtatttatt ttggctgta gaggaaaaa tcaacaaaa agcgggaac cggaatctt ttaaagatt atctaacag gaaacaaaa atcttaaac cttttccgg ttgccatgt cgctaattg caagttaca tatcagggg tgacaatgc ttggaaccc ccgccgaag tgtacaatt ttggagctc agtattggt taattgttt ttgcaatgt ccctcagtg gtctcacag tggctaccg tttatctac cagtattca ccaagctgc aagtttgtt cctcatacg agtatttgg agctaagat tcagcaaga tcctgcgaa caatagcgt cccttttat tcctcttag acgaaatca tgtttttac caatgttga tctacgttc cgtcccttg catttaatc aagtctctg gggcaaatc tacatttga tcggtgcta ttgtctgca cggtttgcg ttttttaca caattttgg gtggtttga gggctgtgg tttacaccg acacttggc aaactgtag ttatgttta tttccgaca tggtagtgg taattttgg gagtcgttg ccatcggag gaatgagtg ttattatcg aacgatcct ctgctggta acaggatcc agtcactga attttaatc ccagtatgt acgaaagat atacagtat ttagcgttc taattggag gattcactt actggactt ctttcaatg cggtcaatc agacaatgg tgcagagat acttatcat taccaaata acaaagagg ctagaatag ccatagcta tttttacag taggagttg tatttttta tatcaatgt gttgtttcg ctggtcttc ttctatttg gcacatatc atgactgtg atcctctag cagcaaaca gaatatcaa acagtgacc agcttcttc ctctgtttg ttgttgaaa ctgcgggac atttaagag gcgttcctg ggcttttta ttgctggtg tttttggag ctgcattaa gttctttat cggttgttc taaactcat cagctgttg ttttgttgg aagactttt tccgcggag tttgtcatt ttagtataa cggagagac agggcaaac tatttacga aaagtataa tattaattc taggaattg cttctgtat gtcttgttt ttgttgttg aaaaaatgg gtagcgtct ttgcagttg ccacttcgc tatctgcaa ttgttgcag gaacaatgt ttggtgttt ttacgcttg gaatgctag taccttggg ctaacgtca agggcgcag tggtgggct cagttagcg gatttttaa tgtcttgta tcgtttctt acggtcaaa tgtatgtcg cttctgcta aaatggtgg tccctcggc cacttcctg tgtctatag aaggctgcg ctgcaactt atggtctca atgttaccg agccgcctg aaatagaat atccggatg aaagcaagg tttttcctc tgttccggt tgtctttcc tgtggataa caccgattg gtgttttca cggtgatcg ctgtgggta ttataacga gttttctga cgggaaaaa ctgatctga agactttgg atccggagc tgatatcgc ctgtaatgc actgggtcc tgcccgagg aggcgcaga attacgcag gatctgctg tgaggaaga ttaaacacc agcgactgt ttggcaatg aggcaattg catcgcctt cagtaacaa tgaatttaa atgtattac tttcatacg gtattattt tcgttactt aatactttt ttacaggag agacgagat acggaaagt cccgacaac agatgaaaa ttgaaaaca tgttataac ttatctcac ttttgtata aatgttgta ctcattaat attgatgtt ttattaata aaatttaaa cgtaaag
- >TC014127 RB
aaggttttg ttgccaatt atttctcgt gttgggagc gaccacgtt gtctattta tttataaaa cgttacatg gactgtatt aacaaccga acaaattcc gagccactt aatcgacca gttgtttga agacatcgt aacaaatca attcgttca aaatgaagc aatccgttt attatttcg atattgtcg attatatta tttttgggg ccatgttat ttttgtcgg ccctaactg gtatttatt ttggctgta gaggaaaaa tcaacaaaa agcgggaac cggaatctt ttaaagatt atctaacag gaaacaaaa atcttaaac cttttccgg ttgccatgt cgctaattg caagttaca tatcagggg tgacaatgc ttggaaccc ccgccgaag tgtacaatt ttggagctc agtattggt taattgttt ttgcaatgt ccctcagtg gtctcacag tggctaccg tttatctac cagtattca ccaagctgc aagtttgtt cctcatacg agtatttgg agctaagat tcagcaaga tcctgcgaa caatagcgt cccttttat tcctcttag acgaaatca tgtttttac caatgttga tctacgttc cgtcccttg catttaatc aagtctctg gggcaaatc tacatttga tcggtgcta ttgtctgca cggtttgcg ttttttaca caattttgg gtggtttga gggctgtgg tttacaccg acacttggc aaactgtag ttatgttta tttccgaca tggtagtgg taattttgg gagtcgttg ccatcggag gaatgagtg ttattatcg aacgatcct ctgctggta acaggatcc agtcactga attttaatc ccagtatgt acgaaagat atacagtat ttagcgttc taattggag gattcactt actggactt ctttcaatg cggtcaatc agacaatgg tgcagagat acttatcat taccaaata acaaagagg ctagaatag ccatagcta tttttacag taggagttg tatttttta tatcaatgt gttgtttcg ctggtcttc ttctatttg gcacatatc atgactgtg atcctctag cagcaaaca gaatatcaa acagtgacc agcttcttc ctctgtttg ttgttgaaa ctgcgggac atttaagag gcgttcctg ggcttttta ttgctggtg tttttggag ctgcattaa gttctttat cggttgttc taaactcat cagctgttg ttttgttgg aagactttt tccgcggag tttgtcatt ttagtataa cggagagac agggcaaac tatttacga aaagtataa tattaattc taggaattg cttctgtat gtcttgttt ttgttgttg aaaaaatgg gtagcgtct ttgcagttg ccacttcgc tatctgcaa ttgttgcag gaacaatgt ttggtgttt ttacgcttg gaatgctag taccttggg ctaacgtca agggcgcag tggtgggct cagttagcg gatttttaa tgtcttgta tcgtttctt acggtcaaa tgtatgtcg cttctgcta aaatggtgg tccctcggc cacttcctg tgtctatag aaggctgcg ctgcaactt atggtctca atgttaccg agccgcctg aaatagaat atccggatg aaagcaagg tttttcctc tgttccggt tgtctttcc tgtggataa caccgattg gtgttttca cggtgatcg ctgtgggta ttataacga gttttctga cgggaaaaa ctgatctga agactttgg atccggagc tgatatcgc ctgtaatgc actgggtcc tgcccgagg aggcgcaga attacgcag gatctgctg tgaggaaga ttaaacacc agcgactgt ttggcaatg aggcaattg catcgcctt cagtaacaa tgaatttaa atgtattac tttcatacg gtgaccacg aagaaggcc gcaccgata cctccgccg ggggccaat caaacccca ctgatttgt cacctgtct tcatcgagc ccaacctcc tcacatact ctacttgga attggaatt ctactggca atgtcactc gaagagtgg ttcttgacg gttccaccg tggacgcca cacggccgc acttggcac caggtgagc gttcgggca ccgacgacg gccgacggg ccacgagcg cttccacag cccgcagag acgaccgcc ggttcagtt gctttcagg agcgcgaag ggcaagttg ctacttaga gccctcgtc gactattga ccgcgtgct ttaatcaaa tataaacaa atattgctg ctttctaca cacatcgct tgtgattgt gccggtttt gaccgcctg tgattgctt gtttgtggg atatttcgt ggggtttgg gcacttctc tcgcccgcc accaagcca aactagact gagtgccat ggtctgacg acgaaacga ccgcacgat tcctacgat ttatttcgg agagtattt tacgctgac tttgtcagt tcaacaaaa gtaagtttg ttgttaatt ttacttatt tgttcaaat tgtgcttaa atacttgtt caggagata aataaacga aaagattaa aa
