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Gene information
General information
TC014124 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6042503..6045416
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014124
    iBeetle screen iB_02251

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014124 RA
      MINEEVTHL LSPPSTPPM KNVSDEEST LQSLRTLLE 
      RKRASPILL TPQPSDSEP EELDYPPKK RFCKNETEL 
      ARHLLSHTP PPEPPRTFS VIMHANKDG SCTRAPIPL 
      EPQMNILRS LKFKMGTRK EKVYVNSKD TGKEPPKQT 
      SKIILPAIA PKFNQPQAV FLAADGTTT FIPGAQLVL 
      LTPTPPQPP PPARRRVYE CTFKGCNKN YFKSSHLKA 
      HNRKHTGEK PYKCEWEDC GRRFSRSDE LSRHKRTHT 
      GEKKYKCSF CDRGFMRSD HLTKHVKRH TKDRAGLQP 
      QKMFVPGLL RPLQPAPA
    CDS sequence
    • >TC014124 RA
      atgatcaac gaagaggtc acacacttg ctgtcacct 
      cccagcaca ccccccatg aaaaacgtg tccgatgaa 
      gagtccact cttcagtcc ttgcggact ctcttggag 
      aggaaacgc gcctcccca atcctcctc accccccaa 
      ccctcggac tcagagccc gaggagttg gactaccct 
      cccaaaaaa cgtttttgt aaaaacgaa accgagttg 
      gcccgccac ctcctcagc cacactcca ccccctgaa 
      ccccctcgc accttctcc gtaataatg cacgccaac 
      aaagacggc tcctgcacc cgcgccccc atccccctg 
      gaaccccaa atgaacatc ctccgcagc ctcaagttc 
      aaaatgggg acgcgcaag gagaaggtc tacgtcaac 
      agcaaagac acgggcaaa gaacccccc aaacagacg 
      tcgaagatc atcctccct gccatcgcc cccaagttc 
      aaccagccc caggctgtc ttcctggcg gctgacggg 
      acgaccacg ttcatcccg ggggcgcag ttggtgttg 
      ctgacgccg acgcccccg cagccgccc ccgccagcc 
      cggcgcagg gtgtacgag tgcacgttc aagggatgc 
      aataagaat tatttcaag tcgtcgcat ctcaaggcg 
      cacaatagg aagcacacg ggggagaag ccctataag 
      tgcgagtgg gaggactgt gggaggagg ttctcgcgg 
      tcggatgag ttgtcgagg cataagagg actcatacg 
      ggcgaaaag aagtacaag tgtagtttt tgcgatagg 
      gggttcatg cggtcggat catttgacc aaacatgtg 
      aaaaggcat acgaaagac agggcgggg ttgcagccc 
      cagaaaatg ttcgtgccg gggctgctg aggccgctg 
      cagccagcg ccagcctaa
    mRNA sequence
    • >TC014124 RA
      ttaagattg caaaatgga acgtaacgg acaacggtt 
      ataaattct taataattt aatgatgag aaaatgccc 
      agctttaca acaggaact ttttttgaa aaaaaaaat 
      tgtttcaaa tcaatttgt caggcatac tgtgctgga 
      ccttgtact tccttgatt agcctacat cttataccc 
      gagtttggg gtcctatac cttatggtt ccaaactaa 
      aaaaagaag aaacaaatg tgtatttaa tgattagta 
      aataattat gaacacgat gaaagcaaa gtaagccat 
      tccttacag cacgtggac aagaagtaa acattttct 
      ctgaaaaca tccgaaaat aaaactgga aaccgggat 
      ttttaaata agatttgat atcgaccat aaacactta 
      attaacata aagtgatca cacacacca catgtcaag 
      gatattcca aatgctttg cttatcaaa attcgccaa 
      tggttggcc aaataatca ccaaatcgt cacttttgg 
      accattatg taataaaaa tcacgccga agacgaaaa 
      ttaatgttg cctcatttt gcacaccta caaaatctt 
      ccgttgtct gcgagccaa tttcttaaa gaggtgact 
      ggcaggatc aggaaaatt tcgggaaat tgtattttt 
      ggtagtgag gccttggag aattttggg aaataatac 
      taagtcctc cacaaataa ctaagagtc ggtgattaa 
      ttcgtacgc caccgatga cagatggcc tgtcagttt 
      gcgtgccta aaaataaaa aaaaatagc ctgaacttg 
      gtatatttg aaaagaaag cgctctgca tgctgtcat 
      cgatcgctc tctggggct tgcctgtga cgtagccct 
      agtcagaca cgctttcat gacgtaaac atcacggcc 
      tcggccgct gatatcagc cgggtagca cgtgaacgt 
      cacgcttgt gatcacgcg tcctctgcg tgatcactc 
      gcacccgat tggaggaaa acgacgagc ccccgcttc 
      ctattggct aatttgccc gttcagcgc caaacacac 
      cccaaaagc tcatgccag agcaataac atcacgaaa 
      cgcaccaac acacagact cattttaat gtaaccact 
      acacagtga acagtgcta ctctagtga tggagtcaa 
      acatgatca acgaagagg tcacacact tgctgtcac 
      ctcccagca cacccccca tgaaaaacg tgtccgatg 
      aagagtcca ctcttcagt ccttgcgga ctctcttgg 
      agaggaaac gcgcctccc caatcctcc tcacccccc 
      aaccctcgg actcagagc ccgaggagt tggactacc 
      ctcccaaaa aacgttttt gtaaaaacg aaaccgagt 
      tggcccgcc acctcctca gccacactc caccccctg 
      aaccccctc gcaccttct ccgtaataa tgcacgcca 
      acaaagacg gctcctgca cccgcgccc ccatccccc 
      tggaacccc aaatgaaca tcctccgca gcctcaagt 
      tcaaaatgg ggacgcgca aggagaagg tctacgtca 
      acagcaaag acacgggca aagaacccc ccaaacaga 
      cgtcgaaga tcatcctcc ctgccatcg cccccaagt 
      tcaaccagc cccaggctg tcttcctgg cggctgacg 
      ggacgacca cgttcatcc cgggggcgc agttggtgt 
      tgctgacgc cgacgcccc cgcagccgc ccccgccag 
      cccggcgca gggtgtacg agtgcacgt tcaagggat 
      gcaataaga attatttca agtcgtcgc atctcaagg 
      cgcacaata ggaagcaca cgggggaga agccctata 
      agtgcgagt gggaggact gtgggagga ggttctcgc 
      ggtcggatg agttgtcga ggcataaga ggactcata 
      cgggcgaaa agaagtaca agtgtagtt tttgcgata 
      gggggttca tgcggtcgg atcatttga ccaaacatg 
      tgaaaaggc atacgaaag acagggcgg ggttgcagc 
      cccagaaaa tgttcgtgc cggggctgc tgaggccgc 
      tgcagccag cgccagcct aagactttc tctcgatta 
      tttttcgtt gctgccgat cttttagtt taatgcaaa 
      ttcgcacga ctgatttcg cgttgtaaa aaagacatt 
      ccactcttc atcgggtca attgttaca tttacaatg 
      ttatctgtg attttttta tattattta ttttaggat 
      agtgaacac taactgtat taagtcact tgtgtatta 
      tgtacctat gttattgta tttgtaaga aagaataga 
      tttttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY