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Gene information
General information
TC014119 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5888435..5895185
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014119
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02250

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014119 RA
      MAGTLARFT NVARSHYGL VATTCSNAQ VALFSTPPP 
      GTPPPQTKT TFGPLSDED RIFTNLYGK HDWRLKGAL 
      KRGDWYKTK EILLKGSDW IINEIKTSG LRGRGGAGF 
      PSGMKWSFM NKPGDGRPK YLVVNADEG EPGTCKDRE 
      IMRHDPHKL VEGCLIAGR AMGAKAAYI YIRGEFYNE 
      ASNMQVAIA EAYQAGLLG KNACGSGYD FDVFMHRGA 
      GAYICGEET ALIESIEGK QGKPRLKPP FPADVGVFG 
      CPTTVTNVE TVAVSPTIC RRGGTWFAS FGRTRNSGT 
      KLFNISGHV NRPCTVEEE MSITFKELV ERHAGGIIG 
      GWDNLLAII PGGSSTPLI PKSVCEDVI MDFDGLVAA 
      QTSLGTAAI IVMNKQTDI VKAIARLIM FYKHESCGQ 
      CTPCREGIN WMNKIMYRF VDGNAKPEE IDMLWELSK 
      QIEGHTICA LGDGAAWPV QGLIRHFRP ELEERMRKF 
      HEQNRSAAR C
    CDS sequence
    • >TC014119 RA
      atggcaggc acattagct agatttaca aatgtagca 
      agaagtcat tatggactc gttgctaca acttgcagc 
      aatgcccaa gtggcactc ttttccaca cctcctcca 
      ggcactcca ccacctcaa acaaagaca acttttggc 
      ccactttcc gacgaggac cgcatattt acaaacttg 
      tatggcaaa catgactgg cgtttgaaa ggggccctg 
      aagagaggt gactggtac aaaacgaag gaaatttta 
      cttaagggc tcagactgg atcataaat gagatcaaa 
      acatctggt ctacgtggt agaggaggc gccggtttc 
      ccctcaggc atgaagtgg tcgttcatg aacaaaccg 
      ggagacggc cgtcccaaa tacctcgtc gtcaatgct 
      gacgaggga gaacctggc acttgcaaa gacagggaa 
      attatgaga catgatcct cacaaattg gtagagggc 
      tgtcttatt gccggcagg gcaatgggg gcgaaagcg 
      gcttatatt tacattaga ggcgaattt tacaatgaa 
      gcatcgaat atgcaagtt gctatagct gaagcttat 
      caagctggc ttactgggc aaaaatgca tgtggttct 
      ggatacgat ttcgatgtt tttatgcat agaggcgca 
      ggagcgtat atttgtggc gaggaaact gcgctgatt 
      gaatctatt gaaggaaag cagggtaaa ccgaggttg 
      aagcctcct ttcccggct gatgtgggg gtttttggg 
      tgccccact actgtgact aacgttgaa actgtcgcc 
      gtgtcaccc acaatttgt cgtcgtgga ggaacatgg 
      tttgcctca tttggcaga acccgaaat tcgggaact 
      aaacttttc aatatctct ggtcacgtc aaccgacct 
      tgcaccgtt gaagaagaa atgagtatt acattcaaa 
      gaattagta gaacgccac gctggtggt atcattggg 
      ggttgggac aaccttttg gccatcatt cctggtggt 
      tcttccaca ccgcttata ccgaaaagt gtttgcgag 
      gacgttatc atggatttc gacggcctg gtcgcggcc 
      cagacaagc ttgggaacc gctgccatc attgtgatg 
      aacaagcaa accgatatt gtcaaagct atcgccaga 
      ttgatcatg ttttacaag cacgagtcc tgcggccag 
      tgcactcct tgccgcgaa ggaatcaat tggatgaac 
      aaaatcatg tacagattt gttgacggc aatgccaaa 
      cccgaggaa atcgacatg ttgtgggag ctgagtaag 
      cagatcgaa gggcacacc atttgcgct ctcggcgac 
      ggtgctgcg tggccagtt cagggactc atcaggcat 
      tttagacca gaattagaa gaaagaatg aggaagttc 
      catgagcaa aataggagc gctgctcgg tgttaa
    mRNA sequence
    • >TC014119 RA
      gaggaaaag tccatatgg tgtgacgta aaatccaaa 
      attaaaata cagaaaata aaagcacac acgaaaaca 
      caaaaaata agggttatc aaaacaagc acaaaataa 
      taataaaca ctaggaaca aaccacaag aaatatcgt 
      cgagattgt tacagacga gagaccact gttacgtaa 
      gatgtcaat aaaaacata aacacatgt tcgaaagcg 
      tctacaaca cgttcgggc accacctca ccactccgc 
      ggtttacaa cgcactggt gagcagctg cgaataagg 
      ctacagtta gcctgcgcg tcaccgttt gacgtgcca 
      acgtcaaaa tggcaggca cattagcta gatttacaa 
      atgtagcaa gaagtcatt atggactcg ttgctacaa 
      cttgcagca atgcccaag tggcactct tttccacac 
      ctcctccag gcactccac cacctcaaa caaagacaa 
      cttttggcc cactttccg acgaggacc gcatattta 
      caaacttgt atggcaaac atgactggc gtttgaaag 
      gggccctga agagaggtg actggtaca aaacgaagg 
      aaattttac ttaagggct cagactgga tcataaatg 
      agatcaaaa catctggtc tacgtggta gaggaggcg 
      ccggtttcc cctcaggca tgaagtggt cgttcatga 
      acaaaccgg gagacggcc gtcccaaat acctcgtcg 
      tcaatgctg acgagggag aacctggca cttgcaaag 
      acagggaaa ttatgagac atgatcctc acaaattgg 
      tagagggct gtcttattg ccggcaggg caatggggg 
      cgaaagcgg cttatattt acattagag gcgaatttt 
      acaatgaag catcgaata tgcaagttg ctatagctg 
      aagcttatc aagctggct tactgggca aaaatgcat 
      gtggttctg gatacgatt tcgatgttt ttatgcata 
      gaggcgcag gagcgtata tttgtggcg aggaaactg 
      cgctgattg aatctattg aaggaaagc agggtaaac 
      cgaggttga agcctcctt tcccggctg atgtggggg 
      tttttgggt gccccacta ctgtgacta acgttgaaa 
      ctgtcgccg tgtcaccca caatttgtc gtcgtggag 
      gaacatggt ttgcctcat ttggcagaa cccgaaatt 
      cgggaacta aacttttca atatctctg gtcacgtca 
      accgacctt gcaccgttg aagaagaaa tgagtatta 
      cattcaaag aattagtag aacgccacg ctggtggta 
      tcattgggg gttgggaca accttttgg ccatcattc 
      ctggtggtt cttccacac cgcttatac cgaaaagtg 
      tttgcgagg acgttatca tggatttcg acggcctgg 
      tcgcggccc agacaagct tgggaaccg ctgccatca 
      ttgtgatga acaagcaaa ccgatattg tcaaagcta 
      tcgccagat tgatcatgt tttacaagc acgagtcct 
      gcggccagt gcactcctt gccgcgaag gaatcaatt 
      ggatgaaca aaatcatgt acagatttg ttgacggca 
      atgccaaac ccgaggaaa tcgacatgt tgtgggagc 
      tgagtaagc agatcgaag ggcacacca tttgcgctc 
      tcggcgacg gtgctgcgt ggccagttc agggactca 
      tcaggcatt ttagaccag aattagaag aaagaatga 
      ggaagttcc atgagcaaa ataggagcg ctgctcggt 
      gttaatttg tagcatttc ataggttta attaactgg 
      aaaaaaact aaaaacagc gaggtatct acgtttact 
      ttttaagta tttatgtta caatgtcta gaggttttg 
      aattgtttg gccaacatg tcgacaact atcagctgg 
      ggcgcttta acaactttg gttttgttg aatgttggc 
      attttatta atttttact tttgaatgt atataatta 
      tgtaatatt gaagtgaat aaaattata gatcgaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY