Status
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Gene information
General information
TC014113 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5758820..5765628
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014113
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02249

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014113 RA
      MSVNDRRNE EWDKNGPAS DKESQAYDG PPGMDPDGV 
      IDSNWDEVV DNFDDMNLK EELLRGIYA YGFEKPSAI 
      QQRAIIPCV KGHDVIAQA QSGTGKTAT FSISILQQI 
      DTSLRECQA LILAPTREL AQQIQKVVI ALGDFMSAQ 
      CHACIGGTN VREDMRKLE TGVHVVVGT PGRVYDMIN 
      RRSLRANFI KMFVLDEAD EMLSRGFKD QIHDVFRML 
      NSDVQVILL SATMPADVL DVTKSFMRN PVRILVKKE 
      ELTLEGIKQ FFVYVERED WKLETLCDL YDTLSITQA 
      VIFCNTRRK VDWLTENMH KRDFTVSAM HGDMEQRER 
      DVIMRQFRT GSSRVLITT DLLARGIDV QQVSLVINY 
      DLPSNRENY IHRIGRGGR FGRKGVAIN FVTEEDKRT 
      LKDIEQFYN TKIDEMPMN VADLI
    CDS sequence
    • >TC014113 RA
      atgtcagtg aacgataga agaaatgaa gaatgggac 
      aaaaacggt cccgcctcc gacaaggaa tctcaggcg 
      tacgacggc cccccgggc atggacccc gatggcgtc 
      atcgactcc aactgggac gaagtcgtc gacaacttc 
      gatgatatg aacttgaag gaggagctg ctccgaggc 
      atttacgct tacggtttc gagaagccc tcggccatc 
      cagcaaagg gccatcata ccatgtgtt aagggccat 
      gacgtcatt gcgcaggcg caatccggg accggcaaa 
      accgccacc ttttcgata tcaattctt cagcagata 
      gacacttcg cttagagaa tgccaagct ttgattttg 
      gctccaacg cgagaatta gcacaacaa attcagaaa 
      gttgtcatc gctcttgga gatttcatg agcgcccaa 
      tgccacgcg tgcatcggg ggcaccaac gttagggaa 
      gatatgagg aagctcgag actggggtc cacgtcgtt 
      gtggggaca ccgggacgg gtctacgat atgatcaac 
      cgaaggtcg ttgcgggct aatttcatc aagatgttt 
      gttctggat gaagccgac gagatgttg tctcgcggg 
      ttcaaggat caaatacac gatgtcttc agaatgctc 
      aactcggac gttcaggtt attcttctc tcggccacg 
      atgcctgct gacgtgttg gacgtaacg aaatcgttt 
      atgcgtaac cccgttcgt atccttgtg aaaaaggaa 
      gaactaacc ctagaaggt atcaagcaa ttcttcgtg 
      tatgttgaa agagaggat tggaagttg gaaacatta 
      tgcgattta tatgacact ctgtccatc actcaagct 
      gttatcttt tgcaatacc cgtcgtaag gtcgattgg 
      cttactgaa aatatgcac aagagggat ttcacggtg 
      tcagctatg cacggtgat atggaacaa agagaacgt 
      gacgtcatt atgcgtcaa tttaggaca ggatcatct 
      agggtttta attacgacg gatctgctt gccagaggg 
      atcgacgtt caacaagtt tcactagtt attaactac 
      gatttgcct tcaaacagg gaaaactac attcatagg 
      attggtcgt ggtggccgc tttggtcgt aagggcgtg 
      gccataaac tttgtgaca gaagaggac aaacgtacg 
      ctcaaggac attgagcag ttttataat accaaaatc 
      gacgaaatg ccaatgaat gtggccgac ctgatataa
    mRNA sequence
    • >TC014113 RA
      ctcaaggta caaatctac tcaacccag ccatcatga 
      ctcatgact gttaatgat gctcctttt tatattatt 
      ataattaga agatatatc gttcattgt gagtgcaat 
      tactatttg gacatagag aaatcggta ataaaattt 
      gagttgaac tgctacggc taatgtgta aactagcct 
      aaccgttgg ctacttcaa tagaaaacg cagttcaac 
      taaaatagc ggctagttt acaaactag ttaagaatc 
      ttgaaagac aaattgcta gatagtttg taaactata 
      cacggcagt ttggccaat atgaaaacc aaattttct 
      agtaacaca aaacaacca atgcttcgg ctagtttac 
      aaactagct agttaattt tactaaatt agccgtcga 
      gttggggtt atgagatat ttttattta tggggtaaa 
      attcttgaa aagcaaggc aaaaccgcg tgtaatcct 
      aatttatta ataaacaca gcttttaca tcaatttca 
      gagacggga gcgttttag cctgtcgaa gcaatttat 
      cagtcttat aaaatcgtt acctacata aatttgtat 
      agcatttgt tgcatttat cttaaaaaa caggacaga 
      aatctttta attgtggtg ctgaacagc cccatgaga 
      cttttttaa atatttttt caaaaaata tcaaaacct 
      ttaaaaaac aaagcagtg ttctaaagt ggtaaggta 
      attacttgt taattgtta ttgttacaa tttgttatg 
      gctacatta gtccaagat tcttaaaaa aaaattgaa 
      tgtggtcaa atgaacata tttgcattc ttcttgcgt 
      tgtcatatt gatattttt atgtgaaac ttgaaacca 
      tcaaatacg tgattaaca aagttcaaa ataccaaag 
      tttgtcact gttttgtag agaatctga aactttgat 
      tttcgtaaa atcataaaa agctcatcg tttgatggt 
      gtttatcta gaaattttt ggccttgaa aatatttac 
      cttaattaa tttattatt tatctaata ttttggact 
      aataaaaag tcacttcct taattccat aaatgtcga 
      cgcttagat tacaatcag aaaatattt tttttcatt 
      taaagaata ataactaaa aattaaatt tatttttgt 
      tgtaacaaa gtgagtagt gagtgttgt gacacattt 
      tcataagta cgattaaaa aactttttt tcggttttt 
      actaaacta gctttaatt ttttttttc taattatcg 
      gtctaaaaa atgattaaa tattccaaa aatgtgtca 
      gaactcagt tccgcattt tttgttata taaatatgt 
      gccaatatt tttttgaat tatgtttaa atttggccg 
      tagtgcaaa cttgatgca caaaaagta ttttgttac 
      ctgttacaa tgttgttag gtttttgta gttattaag 
      ttcaggtgc cacatcaca aataaggag ctattaata 
      cttcaaatg tgttattct taatattga acttttttg 
      ttcgaaatc tataaatac aattatttg tactgtttt 
      tatctcagg aatgaataa atgttgcaa aaaatgaca 
      aaattcgta gtgtagatg tcaagtaac ttttaaatt 
      ttcgcgctg cgatttgac agtgagggc acttccgac 
      gtgggagaa cggtactac aaccttctt cctccgcac 
      taaaaaatc gcacgaggt atttgtaca agtttcccg 
      gatattttg actagcaca agagtccga aagcaaaca 
      attgccaaa cggggatca attctgtgg cacaaacgg 
      gccaaaacg gtgtgtaag tggtttgtg gaaagcccc 
      cagtgtgat ttaccttgg cgtaggaat ataacacct 
      tgcatctag cagcagcag tctttccgt ttataactc 
      aacgcgagt gaatcatgt cagtgaacg atagaagaa 
      atgaagaat gggacaaaa acggtcccg cctccgaca 
      aggaatctc aggcgtacg acggccccc cgggcatgg 
      accccgatg gcgtcatcg actccaact gggacgaag 
      tcgtcgaca acttcgatg atatgaact tgaaggagg 
      agctgctcc gaggcattt acgcttacg gtttcgaga 
      agccctcgg ccatccagc aaagggcca tcataccat 
      gtgttaagg gccatgacg tcattgcgc aggcgcaat 
      ccgggaccg gcaaaaccg ccacctttt cgatatcaa 
      ttcttcagc agatagaca cttcgctta gagaatgcc 
      aagctttga ttttggctc caacgcgag aattagcac 
      aacaaattc agaaagttg tcatcgctc ttggagatt 
      tcatgagcg cccaatgcc acgcgtgca tcgggggca 
      ccaacgtta gggaagata tgaggaagc tcgagactg 
      gggtccacg tcgttgtgg ggacaccgg gacgggtct 
      acgatatga tcaaccgaa ggtcgttgc gggctaatt 
      tcatcaaga tgtttgttc tggatgaag ccgacgaga 
      tgttgtctc gcgggttca aggatcaaa tacacgatg 
      tcttcagaa tgctcaact cggacgttc aggttattc 
      ttctctcgg ccacgatgc ctgctgacg tgttggacg 
      taacgaaat cgtttatgc gtaaccccg ttcgtatcc 
      ttgtgaaaa aggaagaac taaccctag aaggtatca 
      agcaattct tcgtgtatg ttgaaagag aggattgga 
      agttggaaa cattatgcg atttatatg acactctgt 
      ccatcactc aagctgtta tcttttgca atacccgtc 
      gtaaggtcg attggctta ctgaaaata tgcacaaga 
      gggatttca cggtgtcag ctatgcacg gtgatatgg 
      aacaaagag aacgtgacg tcattatgc gtcaattta 
      ggacaggat catctaggg ttttaatta cgacggatc 
      tgcttgcca gagggatcg acgttcaac aagtttcac 
      tagttatta actacgatt tgccttcaa acagggaaa 
      actacattc ataggattg gtcgtggtg gccgctttg 
      gtcgtaagg gcgtggcca taaactttg tgacagaag 
      aggacaaac gtacgctca aggacattg agcagtttt 
      ataatacca aaatcgacg aaatgccaa tgaatgtgg 
      ccgacctga tataaatgc ggtgttgct gtgatccga 
      cgttgccta gagtttctt ttggtgtga gtattagct 
      gcccgtgct cagactgcg acttaatgc gaatattgt 
      gcgccaatt cgtctcttt aggccagcg ccgtattca 
      agcgcagtg ctgcctatc taaataaat ataaacttt 
      ttgagtgtg tcaatttta tatacatat ataaatatt 
      tgtcgtttc ccctctttt gattggttt aacccatac 
      ttgtattag tttttagtt taaattata ttagataat 
      tagtattta taattaagg aggcgcgct cctgtatgg 
      gggctgcgg agtatcctg ttctttccc ttcggggat 
      tagtctttg atcggtttc ttctcgccg cctcccgac 
      agtactgcc gtctccaca ggggtttag ttggcttct 
      ggaaaggtt attataaat ataaaagaa ttagaagga 
      aacatcctt actgatctg tgtttaatg tttggagat 
      tttcttttt ttgtattat aacaaataa aagtgatga 
      tgaaaagtt cgacgtttt attacacct ctccaagaa 
      ccaaaaccc tgaaattat tttgttagc ttgtctttg 
      atcatactt atactcgtt cttatttct aggcagcaa 
      catacctgt gggattttg gaaaataaa gatctttat 
      tccatttta ataagttga tcagtggtt ttgctttac 
      ctaactttc cgttacttc catagtcct tcacacaag 
      atttttatc aagtttttc attaaggat atatttttt 
      tatttattt tatttgtaa cttaaagac tggccacgg 
      taataaact attttccaa tccaatgtt aaccatgtc 
      atttaataa catcttcat aaaaactgc atttgtatc 
      agctgtact tgtaagagt atcatttac gttcatttt 
      ctttgtact tatcatttc aataaagtt tgtgttgta 
      aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY