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Gene information
General information
TC014101 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 4655947..4661315
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014101
    iBeetle screen iB_02248
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014101 RA
      MANKTYWLC CLLLLGIAF AQEEEGGDE ATADELCEN 
      RPADEYFRL TTEGDCRDV VRCTKSGLK QITCPSGLA 
      FDIDKQTCD WKGKVNNCD KLEKPRKVL PNFKTDEPI 
      CPEGKLSCG NGECIDKEL FCNGKPDCK DESDENSCT 
      VETDPNRAP DCDPTQCVL PDCFCSADG TRIPGQLEP 
      ANVPQMITL TFNGAVNVD NIDLYEEIF NGNRANPNG 
      CQIRGTFFV SHKYTNYSA VQDLHRRGH EIAVFSLTH 
      KEDPNYWSQ GSYDDWLAE MAGARLIVE RFANITDGS 
      IIGVRAPYL RVGGNKQFE MMADQFFVY DASITASLG 
      RVPIWPYTL YFRMPHKCN GNAHNCPSR SHPVWEIVM 
      NELDRRDDP TFDESLPGC HMVDSCSNI QTGEQFARL 
      LRHNFNRHL NSNRAPLGL HFHASWLKS KKEFKEELI 
      KFIEEMLQR NDVYFVTNL QVIQWMQNP TELNGLRDF 
      QEWKEKCDI KGQPYCSLP NSCALTTRE LPGETLRLF 
      TCMECPNNY PWILDPTGD GFSVRK
    • >TC014101 RB
      MANKTYWLC CLLLLGIAF AQEEEGGDE ATADELCEN 
      RPADEYFRL TTEGDCRDV VRCDKNSDN NGATRLASV 
      RCPVGLAFD IERQTCDWK TKVNNCDVI EKPRKVLPN 
      FKTDEPICP EGKLSCGNG ECIDKELFC NGKPDCKDE 
      SDENSCTVE TDPNRAPDC DPTQCVLPD CFCSADGTR 
      IPGQLEPAN VPQMITLTF NGAVNVDNI DLYEEIFNG 
      NRANPNGCQ IRGTFFVSH KYTNYSAVQ DLHRRGHEI 
      AVFSLTHKE DPNYWSQGS YDDWLAEMA GARLIVERF 
      ANITDGSII GVRAPYLRV GGNKQFEMM ADQFFVYDA 
      SITASLGRV PIWPYTLYF RMPHKCNGN AHNCPSRSH 
      PVWEIVMNE LDRRDDPTF DESLPGCHM VDSCSNIQT 
      GEQFARLLR HNFNRHLNS NRAPLGLHF HASWLKSKK 
      EFKEELIKF IEEMLQRND VYFVTNLQV IQWMQNPTE 
      LNGLRDFQE WKEKCDIKG QPYCSLPNS CALTTRELP 
      GETLRLFTC MECPNNYPW ILDPTGDGF SVRK
    • >TC014101 RC
      MANKTYWLC CLLLLGIAF AQEEEGGDE ATADELCEN 
      RPADEYFRL TTEGDCRDV VRCDKNSDN NGATRLASV 
      RCPVGLAFD IERQTCDWK TKVNNCDVI EIHSSVQST 
      PVARIVIIS WCTKSGLKQ ITCPSGLAF DIDKQTCDW 
      KGKVNNCDK LEKPRKVLP NFKTDEPIC PEGKLSCGN 
      GECIDKELF CNGKPDCKD ESDENSCTV ETDPNRAPD 
      CDPTQCVLP DCFCSADGT RIPGQLEPA NVPQMITLT 
      FNGAVNVDN IDLYEEIFN GNRANPNGC QIRGTFFVS 
      HKYTNYSAV QDLHRRGHE IAVFSLTHK EDPNYWSQG 
      SYDDWLAEM AGARLIVER FANITDGSI IGVRAPYLR 
      VGGNKQFEM MADQFFVYD ASITASLGR VPIWPYTLY 
      FRMPHKCNG NAHNCPSRS HPVWEIVMN ELDRRDDPT 
      FDESLPGCH MVDSCSNIQ TGEQFARLL RHNFNRHLN 
      SNRAPLGLH FHASWLKSK KEFKEELIK FIEEMLQRN 
      DVYFVTNLQ VIQWMQNPT ELNGLRDFQ EWKEKCDIK 
      GQPYCSLPN SCALTTREL PGETLRLFT CMECPNNYP 
      WILDPTGDG FSVRK
    CDS sequence
    • >TC014101 RA
      atggctaac aaaacatat tggctgtgt tgtttgtta 
      cttcttggg atcgctttt gctcaagag gaagaggga 
      ggcgatgaa gccacagca gatgagctc tgcgaaaac 
      agaccagca gacgaatat ttcagattg accacggaa 
      ggcgactgc agggatgta gtaaggtgt actaagtcc 
      ggtctaaag caaatcact tgtcccagc ggattggca 
      tttgacatc gataaacaa acatgtgac tggaaagga 
      aaagtcaat aactgtgat aaacttgag aagccccgc 
      aaggtcttg cccaatttt aaaacggac gagcccatc 
      tgccctgaa gggaaactg tcttgtggc aatggtgaa 
      tgtatcgac aaggaattg ttctgtaac ggtaaaccc 
      gattgcaag gacgaatct gacgaaaat tcttgcact 
      gtggaaacc gatccaaat cgggcgcct gattgcgac 
      cccacccag tgcgttctc cctgactgc ttctgttcc 
      gccgacggt accaggatc cccggccaa ctggagccc 
      gccaacgtg ccccagatg atcacctta accttcaac 
      ggcgccgtc aatgtcgac aacatcgac ttgtacgag 
      gagatcttc aacggaaac cgcgccaac cccaacggc 
      tgccagatc agggggacc ttcttcgtc tcgcacaaa 
      tacacgaac tactccgcc gtccaagac ctgcaccga 
      cgcggccac gaaatcgcc gtgttttcg ctgacgcac 
      aaagaggac cccaactac tggagccaa gggtcttac 
      gacgactgg cttgccgag atggccgga gcgcgtctg 
      atcgttgag cgcttcgcc aacatcacg gacggttcc 
      atcatcggc gtccgagcc ccgtacctt cgcgtcgga 
      ggcaacaaa cagttcgaa atgatggcc gaccagttc 
      ttcgtctac gacgcctcc atcacggcg tccttgggc 
      cgcgtgccc atctggccc tacaccctc tacttccgg 
      atgccgcac aagtgcaac ggaaacgcg cacaactgc 
      cccagccga agccacccg gtctgggaa atcgtcatg 
      aacgagctg gacagacgt gatgacccc acttttgac 
      gaatctttg cctggttgt cacatggtc gactcctgc 
      tcgaatatt caaacaggc gagcagttt gctcgtctc 
      ctcagacac aacttcaac cgccacttg aacagcaac 
      cgcgcgcct ctcgggctc cacttccac gcctcctgg 
      ctgaagagc aagaaagaa ttcaaggaa gagcttatt 
      aaattcatc gaagagatg ttgcagcgt aatgatgtc 
      tattttgtg accaacctc caggtcatc caatggatg 
      cagaatcca actgagctg aacggcttg cgtgacttc 
      caagaatgg aaggagaaa tgcgatatt aaaggccag 
      ccttactgt tccctgcct aattcgtgc gcgctgact 
      acgagggag cttccagga gagacgctc cgtctgttc 
      acctgcatg gaatgcccc aataattac ccctggatt 
      ttggatccc acaggagac ggcttctcc gttaggaaa 
      taa
    • >TC014101 RB
      atggctaac aaaacatat tggctgtgt tgtttgtta 
      cttcttggg atcgctttt gctcaagag gaagaggga 
      ggcgatgaa gccacagca gatgagctc tgcgaaaac 
      agaccagca gacgaatat ttcagattg accacggaa 
      ggcgactgc agggatgta gtaaggtgc gacaaaaat 
      agtgacaac aacggcgcc acaagactg gcgtctgtt 
      cggtgtcca gttggtcta gcctttgac attgaacgt 
      cagacttgc gattggaaa acaaaagtg aacaactgt 
      gatgttata gaaaagccc cgcaaggtc ttgcccaat 
      tttaaaacg gacgagccc atctgccct gaagggaaa 
      ctgtcttgt ggcaatggt gaatgtatc gacaaggaa 
      ttgttctgt aacggtaaa cccgattgc aaggacgaa 
      tctgacgaa aattcttgc actgtggaa accgatcca 
      aatcgggcg cctgattgc gaccccacc cagtgcgtt 
      ctccctgac tgcttctgt tccgccgac ggtaccagg 
      atccccggc caactggag cccgccaac gtgccccag 
      atgatcacc ttaaccttc aacggcgcc gtcaatgtc 
      gacaacatc gacttgtac gaggagatc ttcaacgga 
      aaccgcgcc aaccccaac ggctgccag atcaggggg 
      accttcttc gtctcgcac aaatacacg aactactcc 
      gccgtccaa gacctgcac cgacgcggc cacgaaatc 
      gccgtgttt tcgctgacg cacaaagag gaccccaac 
      tactggagc caagggtct tacgacgac tggcttgcc 
      gagatggcc ggagcgcgt ctgatcgtt gagcgcttc 
      gccaacatc acggacggt tccatcatc ggcgtccga 
      gccccgtac cttcgcgtc ggaggcaac aaacagttc 
      gaaatgatg gccgaccag ttcttcgtc tacgacgcc 
      tccatcacg gcgtccttg ggccgcgtg cccatctgg 
      ccctacacc ctctacttc cggatgccg cacaagtgc 
      aacggaaac gcgcacaac tgccccagc cgaagccac 
      ccggtctgg gaaatcgtc atgaacgag ctggacaga 
      cgtgatgac cccactttt gacgaatct ttgcctggt 
      tgtcacatg gtcgactcc tgctcgaat attcaaaca 
      ggcgagcag tttgctcgt ctcctcaga cacaacttc 
      aaccgccac ttgaacagc aaccgcgcg cctctcggg 
      ctccacttc cacgcctcc tggctgaag agcaagaaa 
      gaattcaag gaagagctt attaaattc atcgaagag 
      atgttgcag cgtaatgat gtctatttt gtgaccaac 
      ctccaggtc atccaatgg atgcagaat ccaactgag 
      ctgaacggc ttgcgtgac ttccaagaa tggaaggag 
      aaatgcgat attaaaggc cagccttac tgttccctg 
      cctaattcg tgcgcgctg actacgagg gagcttcca 
      ggagagacg ctccgtctg ttcacctgc atggaatgc 
      cccaataat tacccctgg attttggat cccacagga 
      gacggcttc tccgttagg aaataa
    • >TC014101 RC
      atggctaac aaaacatat tggctgtgt tgtttgtta 
      cttcttggg atcgctttt gctcaagag gaagaggga 
      ggcgatgaa gccacagca gatgagctc tgcgaaaac 
      agaccagca gacgaatat ttcagattg accacggaa 
      ggcgactgc agggatgta gtaaggtgc gacaaaaat 
      agtgacaac aacggcgcc acaagactg gcgtctgtt 
      cggtgtcca gttggtcta gcctttgac attgaacgt 
      cagacttgc gattggaaa acaaaagtg aacaactgt 
      gatgttata gaaatccac agttcagta cagtccacg 
      ccagtggct cgtatagtc attatttct tggtgtact 
      aagtccggt ctaaagcaa atcacttgt cccagcgga 
      ttggcattt gacatcgat aaacaaaca tgtgactgg 
      aaaggaaaa gtcaataac tgtgataaa cttgagaag 
      ccccgcaag gtcttgccc aattttaaa acggacgag 
      cccatctgc cctgaaggg aaactgtct tgtggcaat 
      ggtgaatgt atcgacaag gaattgttc tgtaacggt 
      aaacccgat tgcaaggac gaatctgac gaaaattct 
      tgcactgtg gaaaccgat ccaaatcgg gcgcctgat 
      tgcgacccc acccagtgc gttctccct gactgcttc 
      tgttccgcc gacggtacc aggatcccc ggccaactg 
      gagcccgcc aacgtgccc cagatgatc accttaacc 
      ttcaacggc gccgtcaat gtcgacaac atcgacttg 
      tacgaggag atcttcaac ggaaaccgc gccaacccc 
      aacggctgc cagatcagg gggaccttc ttcgtctcg 
      cacaaatac acgaactac tccgccgtc caagacctg 
      caccgacgc ggccacgaa atcgccgtg ttttcgctg 
      acgcacaaa gaggacccc aactactgg agccaaggg 
      tcttacgac gactggctt gccgagatg gccggagcg 
      cgtctgatc gttgagcgc ttcgccaac atcacggac 
      ggttccatc atcggcgtc cgagccccg taccttcgc 
      gtcggaggc aacaaacag ttcgaaatg atggccgac 
      cagttcttc gtctacgac gcctccatc acggcgtcc 
      ttgggccgc gtgcccatc tggccctac accctctac 
      ttccggatg ccgcacaag tgcaacgga aacgcgcac 
      aactgcccc agccgaagc cacccggtc tgggaaatc 
      gtcatgaac gagctggac agacgtgat gaccccact 
      tttgacgaa tctttgcct ggttgtcac atggtcgac 
      tcctgctcg aatattcaa acaggcgag cagtttgct 
      cgtctcctc agacacaac ttcaaccgc cacttgaac 
      agcaaccgc gcgcctctc gggctccac ttccacgcc 
      tcctggctg aagagcaag aaagaattc aaggaagag 
      cttattaaa ttcatcgaa gagatgttg cagcgtaat 
      gatgtctat tttgtgacc aacctccag gtcatccaa 
      tggatgcag aatccaact gagctgaac ggcttgcgt 
      gacttccaa gaatggaag gagaaatgc gatattaaa 
      ggccagcct tactgttcc ctgcctaat tcgtgcgcg 
      ctgactacg agggagctt ccaggagag acgctccgt 
      ctgttcacc tgcatggaa tgccccaat aattacccc 
      tggattttg gatcccaca ggagacggc ttctccgtt 
      aggaaataa
    mRNA sequence
    • >TC014101 RA
      ctcgaaggt tcagcatgg ctaacaaaa catattggc 
      tgtgttgtt tgttacttc ttgggatcg cttttgctc 
      aagaggaag agggaggcg atgaagcca cagcagatg 
      agctctgcg aaaacagac cagcagacg aatatttca 
      gattgacca cggaaggcg actgcaggg atgtagtaa 
      ggtgtacta agtccggtc taaagcaaa tcacttgtc 
      ccagcggat tggcatttg acatcgata aacaaacat 
      gtgactgga aaggaaaag tcaataact gtgataaac 
      ttgagaagc cccgcaagg tcttgccca attttaaaa 
      cggacgagc ccatctgcc ctgaaggga aactgtctt 
      gtggcaatg gtgaatgta tcgacaagg aattgttct 
      gtaacggta aacccgatt gcaaggacg aatctgacg 
      aaaattctt gcactgtgg aaaccgatc caaatcggg 
      cgcctgatt gcgacccca cccagtgcg ttctccctg 
      actgcttct gttccgccg acggtacca ggatccccg 
      gccaactgg agcccgcca acgtgcccc agatgatca 
      ccttaacct tcaacggcg ccgtcaatg tcgacaaca 
      tcgacttgt acgaggaga tcttcaacg gaaaccgcg 
      ccaacccca acggctgcc agatcaggg ggaccttct 
      tcgtctcgc acaaataca cgaactact ccgccgtcc 
      aagacctgc accgacgcg gccacgaaa tcgccgtgt 
      tttcgctga cgcacaaag aggacccca actactgga 
      gccaagggt cttacgacg actggcttg ccgagatgg 
      ccggagcgc gtctgatcg ttgagcgct tcgccaaca 
      tcacggacg gttccatca tcggcgtcc gagccccgt 
      accttcgcg tcggaggca acaaacagt tcgaaatga 
      tggccgacc agttcttcg tctacgacg cctccatca 
      cggcgtcct tgggccgcg tgcccatct ggccctaca 
      ccctctact tccggatgc cgcacaagt gcaacggaa 
      acgcgcaca actgcccca gccgaagcc acccggtct 
      gggaaatcg tcatgaacg agctggaca gacgtgatg 
      accccactt ttgacgaat ctttgcctg gttgtcaca 
      tggtcgact cctgctcga atattcaaa caggcgagc 
      agtttgctc gtctcctca gacacaact tcaaccgcc 
      acttgaaca gcaaccgcg cgcctctcg ggctccact 
      tccacgcct cctggctga agagcaaga aagaattca 
      aggaagagc ttattaaat tcatcgaag agatgttgc 
      agcgtaatg atgtctatt ttgtgacca acctccagg 
      tcatccaat ggatgcaga atccaactg agctgaacg 
      gcttgcgtg acttccaag aatggaagg agaaatgcg 
      atattaaag gccagcctt actgttccc tgcctaatt 
      cgtgcgcgc tgactacga gggagcttc caggagaga 
      cgctccgtc tgttcacct gcatggaat gccccaata 
      attacccct ggattttgg atcccacag gagacggct 
      tctccgtta ggaaataat ttattttat ttttatgtc 
      ttaagtttg attttttta atttaaaag actttgtat 
      atagctatc tataatcaa tttcctatt gcaactttt 
      gataactga atcaacaaa tatagttaa taacttgcc 
      acgagctgt tatacacaa taagactac agatgtgtt 
      atgtaatga aattttgtt aagaaataa aagacacaa 
      aacccattt ttaaaaaaa attaaaatc aaaaagtta 
      cgtggattt tacaaaaaa actgaacgt ggtacgagg 
      aataaaatt ccttaatgt tttatatcg ccaacagtt 
      cgaactcct gcaaaattc aaactacat acaaaaaat 
      catttagtg ttcttgaag gtggtcatt acatatttt 
      agttaactt catgtaata cacttatga ttttaaa
    • >TC014101 RB
      ctcgaaggt tcagcatgg ctaacaaaa catattggc 
      tgtgttgtt tgttacttc ttgggatcg cttttgctc 
      aagaggaag agggaggcg atgaagcca cagcagatg 
      agctctgcg aaaacagac cagcagacg aatatttca 
      gattgacca cggaaggcg actgcaggg atgtagtaa 
      ggtgcgaca aaaatagtg acaacaacg gcgccacaa 
      gactggcgt ctgttcggt gtccagttg gtctagcct 
      ttgacattg aacgtcaga cttgcgatt ggaaaacaa 
      aagtgaaca actgtgatg ttatagaaa agccccgca 
      aggtcttgc ccaatttta aaacggacg agcccatct 
      gccctgaag ggaaactgt cttgtggca atggtgaat 
      gtatcgaca aggaattgt tctgtaacg gtaaacccg 
      attgcaagg acgaatctg acgaaaatt cttgcactg 
      tggaaaccg atccaaatc gggcgcctg attgcgacc 
      ccacccagt gcgttctcc ctgactgct tctgttccg 
      ccgacggta ccaggatcc ccggccaac tggagcccg 
      ccaacgtgc cccagatga tcaccttaa ccttcaacg 
      gcgccgtca atgtcgaca acatcgact tgtacgagg 
      agatcttca acggaaacc gcgccaacc ccaacggct 
      gccagatca gggggacct tcttcgtct cgcacaaat 
      acacgaact actccgccg tccaagacc tgcaccgac 
      gcggccacg aaatcgccg tgttttcgc tgacgcaca 
      aagaggacc ccaactact ggagccaag ggtcttacg 
      acgactggc ttgccgaga tggccggag cgcgtctga 
      tcgttgagc gcttcgcca acatcacgg acggttcca 
      tcatcggcg tccgagccc cgtaccttc gcgtcggag 
      gcaacaaac agttcgaaa tgatggccg accagttct 
      tcgtctacg acgcctcca tcacggcgt ccttgggcc 
      gcgtgccca tctggccct acaccctct acttccgga 
      tgccgcaca agtgcaacg gaaacgcgc acaactgcc 
      ccagccgaa gccacccgg tctgggaaa tcgtcatga 
      acgagctgg acagacgtg atgacccca cttttgacg 
      aatctttgc ctggttgtc acatggtcg actcctgct 
      cgaatattc aaacaggcg agcagtttg ctcgtctcc 
      tcagacaca acttcaacc gccacttga acagcaacc 
      gcgcgcctc tcgggctcc acttccacg cctcctggc 
      tgaagagca agaaagaat tcaaggaag agcttatta 
      aattcatcg aagagatgt tgcagcgta atgatgtct 
      attttgtga ccaacctcc aggtcatcc aatggatgc 
      agaatccaa ctgagctga acggcttgc gtgacttcc 
      aagaatgga aggagaaat gcgatatta aaggccagc 
      cttactgtt ccctgccta attcgtgcg cgctgacta 
      cgagggagc ttccaggag agacgctcc gtctgttca 
      cctgcatgg aatgcccca ataattacc cctggattt 
      tggatccca caggagacg gcttctccg ttaggaaat 
      aatttattt tatttttat gtcttaagt ttgattttt 
      ttaatttaa aagactttg tatatagct atctataat 
      caatttcct attgcaact tttgataac tgaatcaac 
      aaatatagt taataactt gccacgagc tgttataca 
      caataagac tacagatgt gttatgtaa tgaaatttt 
      gttaagaaa taaaagaca caaaaccca tttttaaaa 
      aaaattaaa atcaaaaag ttacgtgga ttttacaaa 
      aaaactgaa cgtggtacg aggaataaa attccttaa 
      tgttttata tcgccaaca gttcgaact cctgcaaaa 
      ttcaaacta catacaaaa aatcattta gtgttcttg 
      aaggtggtc attacatat tttagttaa cttcatgta 
      atacactta tgattttaa a
    • >TC014101 RC
      ctcgaaggt tcagcatgg ctaacaaaa catattggc 
      tgtgttgtt tgttacttc ttgggatcg cttttgctc 
      aagaggaag agggaggcg atgaagcca cagcagatg 
      agctctgcg aaaacagac cagcagacg aatatttca 
      gattgacca cggaaggcg actgcaggg atgtagtaa 
      ggtgcgaca aaaatagtg acaacaacg gcgccacaa 
      gactggcgt ctgttcggt gtccagttg gtctagcct 
      ttgacattg aacgtcaga cttgcgatt ggaaaacaa 
      aagtgaaca actgtgatg ttatagaaa tccacagtt 
      cagtacagt ccacgccag tggctcgta tagtcatta 
      tttcttggt gtactaagt ccggtctaa agcaaatca 
      cttgtccca gcggattgg catttgaca tcgataaac 
      aaacatgtg actggaaag gaaaagtca ataactgtg 
      ataaacttg agaagcccc gcaaggtct tgcccaatt 
      ttaaaacgg acgagccca tctgccctg aagggaaac 
      tgtcttgtg gcaatggtg aatgtatcg acaaggaat 
      tgttctgta acggtaaac ccgattgca aggacgaat 
      ctgacgaaa attcttgca ctgtggaaa ccgatccaa 
      atcgggcgc ctgattgcg accccaccc agtgcgttc 
      tccctgact gcttctgtt ccgccgacg gtaccagga 
      tccccggcc aactggagc ccgccaacg tgccccaga 
      tgatcacct taaccttca acggcgccg tcaatgtcg 
      acaacatcg acttgtacg aggagatct tcaacggaa 
      accgcgcca accccaacg gctgccaga tcaggggga 
      ccttcttcg tctcgcaca aatacacga actactccg 
      ccgtccaag acctgcacc gacgcggcc acgaaatcg 
      ccgtgtttt cgctgacgc acaaagagg accccaact 
      actggagcc aagggtctt acgacgact ggcttgccg 
      agatggccg gagcgcgtc tgatcgttg agcgcttcg 
      ccaacatca cggacggtt ccatcatcg gcgtccgag 
      ccccgtacc ttcgcgtcg gaggcaaca aacagttcg 
      aaatgatgg ccgaccagt tcttcgtct acgacgcct 
      ccatcacgg cgtccttgg gccgcgtgc ccatctggc 
      cctacaccc tctacttcc ggatgccgc acaagtgca 
      acggaaacg cgcacaact gccccagcc gaagccacc 
      cggtctggg aaatcgtca tgaacgagc tggacagac 
      gtgatgacc ccacttttg acgaatctt tgcctggtt 
      gtcacatgg tcgactcct gctcgaata ttcaaacag 
      gcgagcagt ttgctcgtc tcctcagac acaacttca 
      accgccact tgaacagca accgcgcgc ctctcgggc 
      tccacttcc acgcctcct ggctgaaga gcaagaaag 
      aattcaagg aagagctta ttaaattca tcgaagaga 
      tgttgcagc gtaatgatg tctattttg tgaccaacc 
      tccaggtca tccaatgga tgcagaatc caactgagc 
      tgaacggct tgcgtgact tccaagaat ggaaggaga 
      aatgcgata ttaaaggcc agccttact gttccctgc 
      ctaattcgt gcgcgctga ctacgaggg agcttccag 
      gagagacgc tccgtctgt tcacctgca tggaatgcc 
      ccaataatt acccctgga ttttggatc ccacaggag 
      acggcttct ccgttagga aataattta ttttatttt 
      tatgtctta agtttgatt tttttaatt taaaagact 
      ttgtatata gctatctat aatcaattt cctattgca 
      acttttgat aactgaatc aacaaatat agttaataa 
      cttgccacg agctgttat acacaataa gactacaga 
      tgtgttatg taatgaaat tttgttaag aaataaaag 
      acacaaaac ccattttta aaaaaaatt aaaatcaaa 
      aagttacgt ggattttac aaaaaaact gaacgtggt 
      acgaggaat aaaattcct taatgtttt atatcgcca 
      acagttcga actcctgca aaattcaaa ctacataca 
      aaaaatcat ttagtgttc ttgaaggtg gtcattaca 
      tattttagt taacttcat gtaatacac ttatgattt 
      taaa