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Gene information
General information
TC014097 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5606930..5608898
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014097
    iBeetle screen iB_02246

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014097 RA
      MPIENSVTV SPLRQSLAT VGDPNGSFS LSAERKKQA 
      EEELNETPE NLKQELFAL RQMVQNDTN LNIPDDGAF 
      LLRFLRARK FDSKKAFHM LQRYYLMKL KCPELFSCP 
      VPSECEKVF NLQAQNMLL ERDQLGRRV YVIRMDYFD 
      SNVVTIDDI FRTNILALE QIVREPETQ VAGIVLVLD 
      MAGLSLQHA KFFTPYYAK KMVELVQET FPLRFKGFH 
      IVNEPFYFD AVMAVLKPF VKEKIRKRI FLHGSDINA 
      LHGFISNDI LPSEYGGNA GLFDNKSWY MHLLTEEEY 
      FQGLMKYGY KIDNSETDE
    CDS sequence
    • >TC014097 RA
      atgcccatc gaaaattct gttacggtt tcacctctt 
      aggcaatca ctggccact gtaggagat cccaatgga 
      tcattttct ctaagtgcg gaaaggaag aaacaggcc 
      gaagaagaa ttaaacgag acccctgag aatttgaaa 
      caggaactg tttgcttta cgtcaaatg gttcaaaat 
      gataccaat ttaaatata ccagatgat ggcgcgttt 
      ttattgcgc ttcttgcga gctcgaaaa tttgatagc 
      aaaaaagct tttcatatg ctgcaaaga tattacctc 
      atgaaatta aaatgtcct gaattattt tcctgtcca 
      gtaccttct gaatgcgag aaagttttt aatttgcaa 
      gcgcagaat atgctgcta gagagggac caacttgga 
      agaagagtt tatgtaatt cgaatggat tacttcgac 
      tctaatgtt gttactatt gatgatatt tttcgtaca 
      aatattttg gctcttgaa caaatcgtt cgtgaaccg 
      gaaacgcaa gtcgctggg attgtttta gtcctggac 
      atggcaggg ttaagtcta caacacgcg aaatttttt 
      actccgtat tacgcaaag aaaatggtg gaattagtc 
      caagaaaca tttcctctc agatttaag ggcttccat 
      attgtcaac gagcctttc tatttcgat gctgtgatg 
      gctgtgctt aaaccgttt gtgaaagag aaaatcaga 
      aaaagaata tttttacac ggtagtgac ataaacgct 
      cttcatggt tttatctca aatgatata ttaccatct 
      gaatatggg ggcaatgcg ggccttttt gataacaag 
      tcctggtat atgcatcta cttacagag gaagagtat 
      tttcaaggc ttgatgaaa tatggatac aagattgac 
      aattctgag acagacgaa taa
    mRNA sequence
    • >TC014097 RA
      actctaaca aataatggt agatcgaat tattctaaa 
      ttagttttc caatttata gcttcgtgc ataactgca 
      tcatttatg tcattatta ttctttgac gagaggcaa 
      tatatcagc catcatatc aaatttaac agctaataa 
      ctcaatagt tagcagtgt aactcagtt ttctgcgca 
      atcctgcaa cgcatcctg tgagggttt cctgataag 
      tcaataaca cgattcggt gtttatcaa gcgacggtg 
      aaagtaaat caagctcag gataaaccg aagtatatt 
      atattatgc tgtaatagc tcctgaaaa ctggtggaa 
      aaattctca cagagctga acaatgccc atcgaaaat 
      tctgttacg gtttcacct cttaggcaa tcactggcc 
      actgtagga gatcccaat ggatcattt tctctaagt 
      gcggaaagg aagaaacag gccgaagaa gaattaaac 
      gagacccct gagaatttg aaacaggaa ctgtttgct 
      ttacgtcaa atggttcaa aatgatacc aatttaaat 
      ataccagat gatggcgcg tttttattg cgcttcttg 
      cgagctcga aaatttgat agcaaaaaa gcttttcat 
      atgctgcaa agatattac ctcatgaaa ttaaaatgt 
      cctgaatta ttttcctgt ccagtacct tctgaatgc 
      gagaaagtt tttaatttg caagcgcag aatatgctg 
      ctagagagg gaccaactt ggaagaaga gtttatgta 
      attcgaatg gattacttc gactctaat gttgttact 
      attgatgat atttttcgt acaaatatt ttggctctt 
      gaacaaatc gttcgtgaa ccggaaacg caagtcgct 
      gggattgtt ttagtcctg gacatggca gggttaagt 
      ctacaacac gcgaaattt tttactccg tattacgca 
      aagaaaatg gtggaatta gtccaagaa acatttcct 
      ctcagattt aagggcttc catattgtc aacgagcct 
      ttctatttc gatgctgtg atggctgtg cttaaaccg 
      tttgtgaaa gagaaaatc agaaaaaga atattttta 
      cacggtagt gacataaac gctcttcat ggttttatc 
      tcaaatgat atattacca tctgaatat gggggcaat 
      gcgggcctt tttgataac aagtcctgg tatatgcat 
      ctacttaca gaggaagag tattttcaa ggcttgatg 
      aaatatgga tacaagatt gacaattct gagacagac 
      gaataaaat tttgcaact ggcaataat aataatcaa 
      acttgtggg tttatagca agagttttg aatcatgct 
      tgccgttag aaagctcaa ggattttta taaatgatt 
      gtagctctt ggtatactc ctagtgata gggtaaaaa 
      gtattatat ttattttgt taacgcttg gacgcctgt 
      tacctacaa attgttaag cttcacaaa tttattata 
      tgagaaaat aactttgtt atgtaaatt ccattgaga 
      aattagttt aatacagct gtataatcg tattctcac 
      aatgagata atagagagt atttttgaa gtaactaaa 
      cagtgttta ccgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY