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Gene information
General information
TC014082 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 4426892..4427959
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014082
    iBeetle screen iB_02243
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014082 RA
      MADGRRKVI VDVDVGTDD FLALLILLN AEKRRQIKI 
      EAIVCSMGN TAVENVCVN VMRLLEAVE RTDIPVFKG 
      ATKQLIPPT HEIRLFHGK DGFGDLGLK GRPHMEAIK 
      EPAASKIAE LIVGNPGEI SLICVAPLT NVALALRLY 
      DNFADSIKD LWIMGGNYT AVGNITPTA EYNFYIDPE 
      AAFIVLDTV KKPIFILTW ETCLYPKIT FDWRFKVFG 
      AKKNPAIEL LNLAERSVY KEDEKIWLP CDAFLAAAF 
      LKPEIITKK SAHYASVEL HGSQTRGQI VLDHLKTKK 
      ENVTIIEQF DAEAFQQIL MDL
    CDS sequence
    • >TC014082 RA
      atggcggac ggtaggcga aaagtcatt gtcgacgtg 
      gacgtcggc accgacgat ttcctcgca cttctgatc 
      ctcctaaat gccgaaaaa aggcgtcaa atcaaaatt 
      gaagccatc gtgtgttcg atggggaac accgccgtg 
      gaaaatgtt tgcgttaac gtgatgagg ctcctcgag 
      gcagtggaa cgcacggac atcccggtg tttaaaggc 
      gccacgaaa caactcata ccaccaacg cacgaaatt 
      cgacttttc catgggaag gacgggttc ggggatttg 
      gggcttaaa ggaaggccg catatggaa gcaattaaa 
      gaaccggcg gcttccaaa attgcggaa cttattgtg 
      gggaatccc ggcgaaatc agcctcatt tgcgtggcc 
      cccctgacc aatgtcgcc ctcgcgttg cgactctac 
      gacaatttt gcggactct atcaaggat ttgtggata 
      atggggggc aattacacc gcagttgga aacatcaca 
      cccaccgca gagtacaat ttttacatt gaccctgaa 
      gctgcgttt atcgtgctc gacacggtc aaaaaaccg 
      atttttatc ctcacatgg gagacgtgc ctttatcca 
      aaaatcaca ttcgattgg cgcttcaag gttttcggc 
      gccaagaaa aacccagct attgaatta ctaaaccta 
      gctgaaaga agtgtttac aaggaagac gagaaaatt 
      tggctgcct tgcgacgct tttctcgca gcggcgttc 
      ctcaaaccc gaaataatc acgaaaaaa agcgctcat 
      tatgcaagt gttgagctt cacgggagt cagacacgg 
      gggcaaatc gtgctcgac catttgaaa accaagaaa 
      gaaaacgtt acgattatc gaacagttt gatgctgag 
      gcattccag caaatactc atggatttg taa
    mRNA sequence
    • >TC014082 RA
      tatagtttg ataaaagtg tccatggcg gacggtagg 
      cgaaaagtc attgtcgac gtggacgtc ggcaccgac 
      gatttcctc gcacttctg atcctccta aatgccgaa 
      aaaaggcgt caaatcaaa attgaagcc atcgtgtgt 
      tcgatgggg aacaccgcc gtggaaaat gtttgcgtt 
      aacgtgatg aggctcctc gaggcagtg gaacgcacg 
      gacatcccg gtgtttaaa ggcgccacg aaacaactc 
      ataccacca acgcacgaa attcgactt ttccatggg 
      aaggacggg ttcggggat ttggggctt aaaggaagg 
      ccgcatatg gaagcaatt aaagaaccg gcggcttcc 
      aaaattgcg gaacttatt gtggggaat cccggcgaa 
      atcagcctc atttgcgtg gcccccctg accaatgtc 
      gccctcgcg ttgcgactc tacgacaat tttgcggac 
      tctatcaag gatttgtgg ataatgggg ggcaattac 
      accgcagtt ggaaacatc acacccacc gcagagtac 
      aatttttac attgaccct gaagctgcg tttatcgtg 
      ctcgacacg gtcaaaaaa ccgattttt atcctcaca 
      tgggagacg tgcctttat ccaaaaatc acattcgat 
      tggcgcttc aaggttttc ggcgccaag aaaaaccca 
      gctattgaa ttactaaac ctagctgaa agaagtgtt 
      tacaaggaa gacgagaaa atttggctg ccttgcgac 
      gcttttctc gcagcggcg ttcctcaaa cccgaaata 
      atcacgaaa aaaagcgct cattatgca agtgttgag 
      cttcacggg agtcagaca cgggggcaa atcgtgctc 
      gaccatttg aaaaccaag aaagaaaac gttacgatt 
      atcgaacag tttgatgct gaggcattc cagcaaata 
      ctcatggat ttgtaaaaa aaataaacg ttaaactag 
      act