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Gene information
General information
TC014078 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 4367832..4370486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014078
    iBeetle screen iB_02242
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014078 RA
      MEETLQRPA LSEDEVSQI LRKKLESDK FIIVSFTLD 
      MLGEWIGLC GDHAILNVN IKPGNDLKT SETRFTFFV 
      KLFPRVPFV IEFIEGTGA FKKEIFMYK LFAVMEDEE 
      IKTLDECIP RCYFASDKY IVMDNLIEK GYKSLNKHE 
      VFDWDSVKV ALEALARLH ASSLLYEEK KSRRLTDLY 
      EFAESYYND REDFPLKKE INAAITGVL NSIELFNFP 
      KKLISGKDF RTVLSQVCP QVYDLVKPS KKFRNVVCH 
      GDIWATNVV IKHDENNKP VKGRLVDFQ CARYAPPAQ 
      DVMSLIHLT TSREFRKRF MYNLIGIYY TDLEKHVTL 
      GGYDMKKII PFEEFLASC EEQKLFGMI QAATYFPLI 
      LLDGQTVKE YFGDQEKNK KSLFEDRSV LVREYMDKD 
      EKYKNRIRD SIQDLKDYC DYL
    CDS sequence
    • >TC014078 RA
      atggaagaa acattgcaa agacccgct ctaagtgaa 
      gatgaagtg tctcaaatt ctccgcaag aagcttgaa 
      tcggacaag tttattatt gtgagtttc actcttgat 
      atgttgggc gaatggatt ggattatgt ggtgaccat 
      gcgattttg aacgtcaat ataaagcct ggcaatgac 
      ttgaaaact tccgagaca cgtttcaca ttcttcgta 
      aaacttttt ccccgagtg cccttcgtg attgaattt 
      atcgaaggg acgggtgcc ttcaagaag gagattttc 
      atgtacaaa ctcttcgcc gtaatggaa gatgaagaa 
      atcaaaact ttggacgag tgcatcccc cgatgttat 
      ttcgcttcg gataagtac atcgtgatg gataacttg 
      atagaaaag ggttacaaa agtctaaat aaacacgaa 
      gttttcgat tgggattcg gttaaagtc gctttggaa 
      gctctagca cggctgcac gcctcaagt ctgctttac 
      gaagagaag aaatcgcgt cgattgacc gatctttat 
      gaatttgcc gaaagttat tacaacgac cgtgaggat 
      ttccccttg aagaaggaa atcaacgct gcaatcacc 
      ggtgttttg aactcaatt gagctcttt aattttccc 
      aaaaagctc atttcgggg aaagacttt cgcacagtc 
      ctctcgcaa gtctgtcct caagtttac gatttggtg 
      aaaccttcg aaaaaattc cgcaatgtt gtgtgccac 
      ggtgatatt tgggccaca aatgttgtc atcaaacac 
      gacgagaat aataaacct gtcaaagga agattagtg 
      gattttcag tgtgccaga tatgcgccc ccagctcag 
      gatgtgatg tctctgata catttaaca acttcgagg 
      gagtttaga aagaggttt atgtataat ttgattggg 
      atttactac accgatttg gagaaacac gtgacactt 
      ggagggtac gatatgaag aagattatt ccgttcgag 
      gagttttta gcaagttgt gaggaacaa aaactgttc 
      gggatgatc caagcggcg acgtatttt cccttaatt 
      ttgttggat ggtcagacc gtgaaggag tatttcgga 
      gatcaagag aaaaataaa aaaagtttg ttcgaagat 
      aggagcgtc cttgttaga gagtatatg gacaaggac 
      gaaaaatat aaaaatcgg attcgggat tcgattcag 
      gaccttaag gattattgt gattatttg tag
    mRNA sequence
    • >TC014078 RA
      catctttag tacaagctg tgataaaat ggaagaaac 
      attgcaaag acccgctct aagtgaaga tgaagtgtc 
      tcaaattct ccgcaagaa gcttgaatc ggacaagtt 
      tattattgt gagtttcac tcttgatat gttgggcga 
      atggattgg attatgtgg tgaccatgc gattttgaa 
      cgtcaatat aaagcctgg caatgactt gaaaacttc 
      cgagacacg tttcacatt cttcgtaaa actttttcc 
      ccgagtgcc cttcgtgat tgaatttat cgaagggac 
      gggtgcctt caagaagga gattttcat gtacaaact 
      cttcgccgt aatggaaga tgaagaaat caaaacttt 
      ggacgagtg catcccccg atgttattt cgcttcgga 
      taagtacat cgtgatgga taacttgat agaaaaggg 
      ttacaaaag tctaaataa acacgaagt tttcgattg 
      ggattcggt taaagtcgc tttggaagc tctagcacg 
      gctgcacgc ctcaagtct gctttacga agagaagaa 
      atcgcgtcg attgaccga tctttatga atttgccga 
      aagttatta caacgaccg tgaggattt ccccttgaa 
      gaaggaaat caacgctgc aatcaccgg tgttttgaa 
      ctcaattga gctctttaa ttttcccaa aaagctcat 
      ttcggggaa agactttcg cacagtcct ctcgcaagt 
      ctgtcctca agtttacga tttggtgaa accttcgaa 
      aaaattccg caatgttgt gtgccacgg tgatatttg 
      ggccacaaa tgttgtcat caaacacga cgagaataa 
      taaacctgt caaaggaag attagtgga ttttcagtg 
      tgccagata tgcgccccc agctcagga tgtgatgtc 
      tctgataca tttaacaac ttcgaggga gtttagaaa 
      gaggtttat gtataattt gattgggat ttactacac 
      cgatttgga gaaacacgt gacacttgg agggtacga 
      tatgaagaa gattattcc gttcgagga gtttttagc 
      aagttgtga ggaacaaaa actgttcgg gatgatcca 
      agcggcgac gtattttcc cttaatttt gttggatgg 
      tcagaccgt gaaggagta tttcggaga tcaagagaa 
      aaataaaaa aagtttgtt cgaagatag gagcgtcct 
      tgttagaga gtatatgga caaggacga aaaatataa 
      aaatcggat tcgggattc gattcagga ccttaagga 
      ttattgtga ttatttgta ggaaataaa tttatttcg 
      acaattatt tcacgttac atttgaccc tcaaaaaat 
      tccctaagc tcggcaatg gcctcaaaa ttcattttc 
      ctaaaaatc gggtccttt tgacaaact tcagtaata 
      aaatcgtat ttctccttg aaaaacacc gacaggact 
      ttgtcctga ttcgcaaac agttcacgt gcgacttct 
      tcagacatt acgacaatt tggaatagt ataatagct 
      tggaccata gcgtaacgt ttataaaag cggcaactt 
      tcatcaaac tcttccctc ttaatcaga tttttaagc 
      ccaaacttg tccaaactc gcgtccagt tccttatag 
      aagaacccc agcacttct tctcgatgc ttatcccta 
      aaatccctg ttggtggtc aaatgcaaa aacgccata 
      acatcctgt gctgggggt ccatatcta caggcctgg 
      aaatcaaca aggacgcaa tttttcgat ttttgctcc 
      attttgaac ataaaattc cccgtccac acatccccg 
      tgacaaatc gtgttgcgg aatctcctc gacggtgtc 
      acatacaat ttctgcaag tccagaact tcgctcact 
      ttttgcttc aaccacccg ctggaaatt ttcattgtg 
      ggctcgtgt gagaggtca ataatggca ttagcgccg 
      gttttgtac gcttccatg ctagtcctc accgattct 
      acatcggaa taaaaggct tcagcgagt tccttcgcc 
      agtttttcg ttcaacttg cacgaggtt ttttcttca 
      aggataagt ccagtggcg tgcaatttt gccaaactc 
      gtgagagcc gatttgaca cagtcaagg cttaagcac 
      tcacgtgaa gacaaagta gtgtagttt ttcgttgac 
      aagtcttcg aaaacaaat ttgtcgtct ttgatcagg 
      tagcaagtt gggatcgtg ttgtccaaa aggctgctg 
      attgagtgt tctttcacc aggtcgcag aatttggta 
      aaaaaacct tgctctttg aaaaatccg ttcacttcc 
      aaaacgaaa tttgtgttc tttgtcagt tacattcga 
      cttggttgt gatcgtaag agtgaaatg atcgcccaa 
      aaaaacact tggagggta tgatatgaa gaagattat 
      tccgtttaa agagttttt agcaagttg tgaggaaca 
      aaaactgtt cgggatgat tcaagcagc gacgtattt 
      tcccttaat tttgttgga taggcagac cgtgaagga 
      gtttttcgg agatcaaga gaaaaataa aaaagtttg 
      tttgaaaat aggagcgtc cttgttaga gagtatatg 
      gacaaggac gaaaaatat aaaaatcgg attcgggat 
      tcgattcag gaccttaag gattattgt