Status
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Gene information
General information
TC034689 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5310073..5330282
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034689
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02241

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head partially anteriornot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034689 RA
      MATPHKKSW KLQELMAHD AKVNCLSLG HKSGRVMVT 
GGDDMKVNL WAIGKHTCF MILSGHTTP IECVQFNQF 
EELVCAGSR AGALKVWDL EAAKLVRTL NGHKSALKC 
VDFHPYGDF LASGSSDCS IKMWDSRKK GCIYTYNGH 
KATINSLKF SPDGHWIAS GGDDATVKI WDLRVGKVL 
KDFGEHLNS VTCVEFHPH EFLLASGSA DRSVQFYDL 
ENFSVVSSE RDLGLVRCL CFNPDGERL FVGLRDYLK 
VVGWEPNRL FDSVMVNWS GVADISIAQ NQLVGASFH 
LTNVQVYVV DLNKVHPLN SSTETPPSP FTPNQSIRK 
SFSKAERPV SLKNRSLDV KTIEESTSG TDPEEESTA 
EVTNLTDYN EIFKGRGLT RTPPPLEQP EPFQRPEHD 
YSDPTEPQI LAEIPTDNF EALSLDNNI NCYSPPPAF 
TSSQYSRSK SNLDQVYVS RLTKQQDRE NNISNINKN 
KNQLTTPRY YLQRQSSCK DVPENAPKA NNSIIKHSV 
SDANISKSS ISSRNSSRK NSFSRPSRN STSVPNVSK 
IPSARNSEK VAPVKTYIT PTPEDVFVS SKLSPDSPS 
EEVEFVPMS MDRPAGLDL DDFLPKNHR TLGFEQQLP 
DMSEAEVLG ALVRGHEPM MAMLVTRQR SLKVVLAQL 
RSKDIKTAT ETAVVMGDL AVLVDLLGV FNCKYTLWN 
LDLCVLILP KIQELLQSK FETYVTTGC NTLKLILKH 
FGPVIKSNV QSPVGSFGV DIPREERYN KSVKCHEVL 
QNLRTFLVK KQSMPGSVG STFREISTL IQNTFD
    CDS sequence
    • >TC034689 RA
      atggcaacc ccccacaag aagtcatgg aagctccag 
gagctcatg gcccacgac gccaaagtc aattgcctc 
tctctgggg cacaagtcg ggccgcgtg atggtgaca 
gggggcgac gacatgaaa gtcaacctg tgggccatc 
ggcaagcac acctgcttc atgattctc agcggccac 
acaaccccc atagagtgc gtccaattc aaccagttt 
gaggagcta gtgtgcgcc ggctcgagg gccggggcc 
ctcaaagtg tgggacttg gaggcggcc aagctggtc 
cgcaccctc aacggccat aaaagcgcc ctcaagtgt 
gtggatttt cacccgtat ggcgatttt ctagcgtct 
gggagctcc gactgttcc atcaaaatg tgggatagt 
cgcaagaag ggctgcatt tacacctac aacggccac 
aaggccacc atcaacagt ctgaagttc agccctgac 
gggcactgg atcgcctca gggggcgac gacgccacg 
gtgaaaatc tgggacttg agggttggg aaggtcctg 
aaggacttt ggggagcat ttgaattcg gtgacttgt 
gtcgagttc catccgcat gagttcttg ctggcatcg 
ggaagtgcc gaccgatct gtgcagttc tacgacttg 
gagaatttt tcggtggtg tcgagtgag agggacttg 
gggttggtg cggtgtttg tgctttaat cccgatggg 
gagaggctg tttgtggga cttagggat tacttgaag 
gttgtgggt tgggagccc aacagactt ttcgactcg 
gtgatggtt aattggagt ggcgttgcc gatatttcc 
atcgctcaa aatcaactg gtcggcgct tccttccac 
ctaaccaac gtccaggtc tacgtcgtc gacttgaac 
aaagtacac cctttgaat tcctcaacg gaaacaccc 
ccgagtcct ttcacacct aaccagagt atcagaaag 
agtttttcg aaagcggaa cgaccggtc agtttgaaa 
aaccgatca ctggacgtc aaaacgata gaagagagc 
acctcgggg accgatccg gaggaagaa tccacggcc 
gaagtgacg aatttaacg gattataac gaaatattc 
aaggggcga ggactgact cggacaccg cctccgctc 
gaacaacct gagccgttt caaaggcct gaacatgac 
tattcggac ccgactgaa cctcagatc ttggccgaa 
attccaaca gacaatttc gaggctctc tccctggac 
aacaacatc aactgttac tcaccaccg cctgcgttc 
acttcttca cagtattcc cgttcaaag tccaatctg 
gatcaggtc tatgtcagc aggctgacg aagcaacaa 
gaccgcgaa aataacatt tccaatatc aataagaat 
aaaaatcaa ttgaccact ccaaggtat tatcttcag 
aggcagagc agttgtaaa gatgtgcct gaaaatgcg 
cccaaagcg aacaacagt attattaaa catagcgtc 
tctgatgcc aatatcagc aagagtagt atatcgtca 
aggaactca tcgagaaag aattccttt tcaagacct 
agtcggaac tctactagt gtacctaat gtttcgaaa 
attcccagc gctaggaat agcgaaaaa gtggcaccg 
gttaaaacc tacattaca ccgacccct gaagatgtg 
tttgttagt tcgaaactt tcgccagat agtccatcc 
gaagaagtg gagtttgtt ccgatgtcg atggacaga 
ccggcagga ctggacttg gacgatttt cttccgaaa 
aaccatcga acgcttggc ttcgagcaa caactgcca 
gatatgtcc gaagctgaa gtgttgggg gctctggtg 
aggggtcac gagccgatg atggcaatg ttggtgact 
cgtcagagg agtttgaaa gtggttttg gctcaattg 
aggagtaag gatattaag acagcgact gagactgca 
gttgtgatg ggtgatttg gccgttttg gtcgatttg 
ttaggagtt tttaattgt aaatatacg ttgtggaat 
ttggatttg tgtgtgctg attttaccc aagattcaa 
gaattgctt cagagtaaa tttgaaacg tacgtgacg 
acgggttgt aacaccctc aagctcatc ctgaagcat 
ttcgggcca gtgatcaag agcaacgtt caatccccg 
gtgggcagc ttcggcgtc gacattcct cgcgaggag 
cgttacaac aaatcggtc aagtgccac gaagtcttg 
caaaacttg cggactttt ctcgtcaaa aaacagtca 
atgcccggt tcagtgggc tccactttt cgcgaaatc 
agtacttta attcagaac accttcgac tga
    mRNA sequence
    • >TC034689 RA
      cataattta atatgggtc gtggctggt gtggaccgt 
gcgcctcct agacgtcac tgtgatgtg tgtgcacgt 
ccttccacc gaaataatt aagaaaatc ggtttttcg 
ctcagttca gttctcttg tgcgcttgt gtagtcctc 
cttaacatc ccgtgcgat agagcagtt ttttgagtg 
cttcaacaa attttttaa accgaaaga tgctcaaac 
catcccatt tattcaatt tacccatca aactcgtct 
atttataaa ccgttcata aacaaactt ccagcgaag 
tagccgacg atggcaacc ccccacaag aagtcatgg 
aagctccag gagctcatg gcccacgac gccaaagtc 
aattgcctc tctctgggg cacaagtcg ggccgcgtg 
atggtgaca gggggcgac gacatgaaa gtcaacctg 
tgggccatc ggcaagcac acctgcttc atgattctc 
agcggccac acaaccccc atagagtgc gtccaattc 
aaccagttt gaggagcta gtgtgcgcc ggctcgagg 
gccggggcc ctcaaagtg tgggacttg gaggcggcc 
aagctggtc cgcaccctc aacggccat aaaagcgcc 
ctcaagtgt gtggatttt cacccgtat ggcgatttt 
ctagcgtct gggagctcc gactgttcc atcaaaatg 
tgggatagt cgcaagaag ggctgcatt tacacctac 
aacggccac aaggccacc atcaacagt ctgaagttc 
agccctgac gggcactgg atcgcctca gggggcgac 
gacgccacg gtgaaaatc tgggacttg agggttggg 
aaggtcctg aaggacttt ggggagcat ttgaattcg 
gtgacttgt gtcgagttc catccgcat gagttcttg 
ctggcatcg ggaagtgcc gaccgatct gtgcagttc 
tacgacttg gagaatttt tcggtggtg tcgagtgag 
agggacttg gggttggtg cggtgtttg tgctttaat 
cccgatggg gagaggctg tttgtggga cttagggat 
tacttgaag gttgtgggt tgggagccc aacagactt 
ttcgactcg gtgatggtt aattggagt ggcgttgcc 
gatatttcc atcgctcaa aatcaactg gtcggcgct 
tccttccac ctaaccaac gtccaggtc tacgtcgtc 
gacttgaac aaagtacac cctttgaat tcctcaacg 
gaaacaccc ccgagtcct ttcacacct aaccagagt 
atcagaaag agtttttcg aaagcggaa cgaccggtc 
agtttgaaa aaccgatca ctggacgtc aaaacgata 
gaagagagc acctcgggg accgatccg gaggaagaa 
tccacggcc gaagtgacg aatttaacg gattataac 
gaaatattc aaggggcga ggactgact cggacaccg 
cctccgctc gaacaacct gagccgttt caaaggcct 
gaacatgac tattcggac ccgactgaa cctcagatc 
ttggccgaa attccaaca gacaatttc gaggctctc 
tccctggac aacaacatc aactgttac tcaccaccg 
cctgcgttc acttcttca cagtattcc cgttcaaag 
tccaatctg gatcaggtc tatgtcagc aggctgacg 
aagcaacaa gaccgcgaa aataacatt tccaatatc 
aataagaat aaaaatcaa ttgaccact ccaaggtat 
tatcttcag aggcagagc agttgtaaa gatgtgcct 
gaaaatgcg cccaaagcg aacaacagt attattaaa 
catagcgtc tctgatgcc aatatcagc aagagtagt 
atatcgtca aggaactca tcgagaaag aattccttt 
tcaagacct agtcggaac tctactagt gtacctaat 
gtttcgaaa attcccagc gctaggaat agcgaaaaa 
gtggcaccg gttaaaacc tacattaca ccgacccct 
gaagatgtg tttgttagt tcgaaactt tcgccagat 
agtccatcc gaagaagtg gagtttgtt ccgatgtcg 
atggacaga ccggcagga ctggacttg gacgatttt 
cttccgaaa aaccatcga acgcttggc ttcgagcaa 
caactgcca gatatgtcc gaagctgaa gtgttgggg 
gctctggtg aggggtcac gagccgatg atggcaatg 
ttggtgact cgtcagagg agtttgaaa gtggttttg 
gctcaattg aggagtaag gatattaag acagcgact 
gagactgca gttgtgatg ggtgatttg gccgttttg 
gtcgatttg ttaggagtt tttaattgt aaatatacg 
ttgtggaat ttggatttg tgtgtgctg attttaccc 
aagattcaa gaattgctt cagagtaaa tttgaaacg 
tacgtgacg acgggttgt aacaccctc aagctcatc 
ctgaagcat ttcgggcca gtgatcaag agcaacgtt 
caatccccg gtgggcagc ttcggcgtc gacattcct 
cgcgaggag cgttacaac aaatcggtc aagtgccac 
gaagtcttg caaaacttg cggactttt ctcgtcaaa 
aaacagtca atgcccggt tcagtgggc tccactttt 
cgcgaaatc agtacttta attcagaac accttcgac 
tgacgaaag cccaaactg actcaccag aaccggagt 
gtttgagaa gtgtctcga aattttaac atgtgattg 
cgtattgga agaaaagac tagaaattc cgtccagtt 
tgctaggag tagaggact aacggttta tgatactta 
acgaagtta ctaaaaggt agttttaga taatttatt 
tcactaaag aagacttag acgacgacg agcggttga 
tggtgcgat tttttcttg cctgtttta acatgtttt 
gtgccattt tgttttatt tgtttctcc cgaacgagt 
tctgatggc ggaattaac ctcaattta tgtcttttt 
tacctacac tagtgctta ataaaccaa actattgta 
acataattt ataataaaa aagtgatta ttaa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY