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Gene information
General information
TC034689 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 4274865..4295074
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034689
    iBeetle screen iB_02241
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head partially anteriornot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034689 RA
      MATPHKKSW KLQELMAHD AKVNCLSLG HKSGRVMVT 
      GGDDMKVNL WAIGKHTCF MILSGHTTP IECVQFNQF 
      EELVCAGSR AGALKVWDL EAAKLVRTL NGHKSALKC 
      VDFHPYGDF LASGSSDCS IKMWDSRKK GCIYTYNGH 
      KATINSLKF SPDGHWIAS GGDDATVKI WDLRVGKVL 
      KDFGEHLNS VTCVEFHPH EFLLASGSA DRSVQFYDL 
      ENFSVVSSE RDLGLVRCL CFNPDGERL FVGLRDYLK 
      VVGWEPNRL FDSVMVNWS GVADISIAQ NQLVGASFH 
      LTNVQVYVV DLNKVHPLN SSTETPPSP FTPNQSIRK 
      SFSKAERPV SLKNRSLDV KTIEESTSG TDPEEESTA 
      EVTNLTDYN EIFKGRGLT RTPPPLEQP EPFQRPEHD 
      YSDPTEPQI LAEIPTDNF EALSLDNNI NCYSPPPAF 
      TSSQYSRSK SNLDQVYVS RLTKQQDRE NNISNINKN 
      KNQLTTPRY YLQRQSSCK DVPENAPKA NNSIIKHSV 
      SDANISKSS ISSRNSSRK NSFSRPSRN STSVPNVSK 
      IPSARNSEK VAPVKTYIT PTPEDVFVS SKLSPDSPS 
      EEVEFVPMS MDRPAGLDL DDFLPKNHR TLGFEQQLP 
      DMSEAEVLG ALVRGHEPM MAMLVTRQR SLKVVLAQL 
      RSKDIKTAT ETAVVMGDL AVLVDLLGV FNCKYTLWN 
      LDLCVLILP KIQELLQSK FETYVTTGC NTLKLILKH 
      FGPVIKSNV QSPVGSFGV DIPREERYN KSVKCHEVL 
      QNLRTFLVK KQSMPGSVG STFREISTL IQNTFD
    CDS sequence
    • >TC034689 RA
      atggcaacc ccccacaag aagtcatgg aagctccag 
      gagctcatg gcccacgac gccaaagtc aattgcctc 
      tctctgggg cacaagtcg ggccgcgtg atggtgaca 
      gggggcgac gacatgaaa gtcaacctg tgggccatc 
      ggcaagcac acctgcttc atgattctc agcggccac 
      acaaccccc atagagtgc gtccaattc aaccagttt 
      gaggagcta gtgtgcgcc ggctcgagg gccggggcc 
      ctcaaagtg tgggacttg gaggcggcc aagctggtc 
      cgcaccctc aacggccat aaaagcgcc ctcaagtgt 
      gtggatttt cacccgtat ggcgatttt ctagcgtct 
      gggagctcc gactgttcc atcaaaatg tgggatagt 
      cgcaagaag ggctgcatt tacacctac aacggccac 
      aaggccacc atcaacagt ctgaagttc agccctgac 
      gggcactgg atcgcctca gggggcgac gacgccacg 
      gtgaaaatc tgggacttg agggttggg aaggtcctg 
      aaggacttt ggggagcat ttgaattcg gtgacttgt 
      gtcgagttc catccgcat gagttcttg ctggcatcg 
      ggaagtgcc gaccgatct gtgcagttc tacgacttg 
      gagaatttt tcggtggtg tcgagtgag agggacttg 
      gggttggtg cggtgtttg tgctttaat cccgatggg 
      gagaggctg tttgtggga cttagggat tacttgaag 
      gttgtgggt tgggagccc aacagactt ttcgactcg 
      gtgatggtt aattggagt ggcgttgcc gatatttcc 
      atcgctcaa aatcaactg gtcggcgct tccttccac 
      ctaaccaac gtccaggtc tacgtcgtc gacttgaac 
      aaagtacac cctttgaat tcctcaacg gaaacaccc 
      ccgagtcct ttcacacct aaccagagt atcagaaag 
      agtttttcg aaagcggaa cgaccggtc agtttgaaa 
      aaccgatca ctggacgtc aaaacgata gaagagagc 
      acctcgggg accgatccg gaggaagaa tccacggcc 
      gaagtgacg aatttaacg gattataac gaaatattc 
      aaggggcga ggactgact cggacaccg cctccgctc 
      gaacaacct gagccgttt caaaggcct gaacatgac 
      tattcggac ccgactgaa cctcagatc ttggccgaa 
      attccaaca gacaatttc gaggctctc tccctggac 
      aacaacatc aactgttac tcaccaccg cctgcgttc 
      acttcttca cagtattcc cgttcaaag tccaatctg 
      gatcaggtc tatgtcagc aggctgacg aagcaacaa 
      gaccgcgaa aataacatt tccaatatc aataagaat 
      aaaaatcaa ttgaccact ccaaggtat tatcttcag 
      aggcagagc agttgtaaa gatgtgcct gaaaatgcg 
      cccaaagcg aacaacagt attattaaa catagcgtc 
      tctgatgcc aatatcagc aagagtagt atatcgtca 
      aggaactca tcgagaaag aattccttt tcaagacct 
      agtcggaac tctactagt gtacctaat gtttcgaaa 
      attcccagc gctaggaat agcgaaaaa gtggcaccg 
      gttaaaacc tacattaca ccgacccct gaagatgtg 
      tttgttagt tcgaaactt tcgccagat agtccatcc 
      gaagaagtg gagtttgtt ccgatgtcg atggacaga 
      ccggcagga ctggacttg gacgatttt cttccgaaa 
      aaccatcga acgcttggc ttcgagcaa caactgcca 
      gatatgtcc gaagctgaa gtgttgggg gctctggtg 
      aggggtcac gagccgatg atggcaatg ttggtgact 
      cgtcagagg agtttgaaa gtggttttg gctcaattg 
      aggagtaag gatattaag acagcgact gagactgca 
      gttgtgatg ggtgatttg gccgttttg gtcgatttg 
      ttaggagtt tttaattgt aaatatacg ttgtggaat 
      ttggatttg tgtgtgctg attttaccc aagattcaa 
      gaattgctt cagagtaaa tttgaaacg tacgtgacg 
      acgggttgt aacaccctc aagctcatc ctgaagcat 
      ttcgggcca gtgatcaag agcaacgtt caatccccg 
      gtgggcagc ttcggcgtc gacattcct cgcgaggag 
      cgttacaac aaatcggtc aagtgccac gaagtcttg 
      caaaacttg cggactttt ctcgtcaaa aaacagtca 
      atgcccggt tcagtgggc tccactttt cgcgaaatc 
      agtacttta attcagaac accttcgac tga
    mRNA sequence
    • >TC034689 RA
      cataattta atatgggtc gtggctggt gtggaccgt 
      gcgcctcct agacgtcac tgtgatgtg tgtgcacgt 
      ccttccacc gaaataatt aagaaaatc ggtttttcg 
      ctcagttca gttctcttg tgcgcttgt gtagtcctc 
      cttaacatc ccgtgcgat agagcagtt ttttgagtg 
      cttcaacaa attttttaa accgaaaga tgctcaaac 
      catcccatt tattcaatt tacccatca aactcgtct 
      atttataaa ccgttcata aacaaactt ccagcgaag 
      tagccgacg atggcaacc ccccacaag aagtcatgg 
      aagctccag gagctcatg gcccacgac gccaaagtc 
      aattgcctc tctctgggg cacaagtcg ggccgcgtg 
      atggtgaca gggggcgac gacatgaaa gtcaacctg 
      tgggccatc ggcaagcac acctgcttc atgattctc 
      agcggccac acaaccccc atagagtgc gtccaattc 
      aaccagttt gaggagcta gtgtgcgcc ggctcgagg 
      gccggggcc ctcaaagtg tgggacttg gaggcggcc 
      aagctggtc cgcaccctc aacggccat aaaagcgcc 
      ctcaagtgt gtggatttt cacccgtat ggcgatttt 
      ctagcgtct gggagctcc gactgttcc atcaaaatg 
      tgggatagt cgcaagaag ggctgcatt tacacctac 
      aacggccac aaggccacc atcaacagt ctgaagttc 
      agccctgac gggcactgg atcgcctca gggggcgac 
      gacgccacg gtgaaaatc tgggacttg agggttggg 
      aaggtcctg aaggacttt ggggagcat ttgaattcg 
      gtgacttgt gtcgagttc catccgcat gagttcttg 
      ctggcatcg ggaagtgcc gaccgatct gtgcagttc 
      tacgacttg gagaatttt tcggtggtg tcgagtgag 
      agggacttg gggttggtg cggtgtttg tgctttaat 
      cccgatggg gagaggctg tttgtggga cttagggat 
      tacttgaag gttgtgggt tgggagccc aacagactt 
      ttcgactcg gtgatggtt aattggagt ggcgttgcc 
      gatatttcc atcgctcaa aatcaactg gtcggcgct 
      tccttccac ctaaccaac gtccaggtc tacgtcgtc 
      gacttgaac aaagtacac cctttgaat tcctcaacg 
      gaaacaccc ccgagtcct ttcacacct aaccagagt 
      atcagaaag agtttttcg aaagcggaa cgaccggtc 
      agtttgaaa aaccgatca ctggacgtc aaaacgata 
      gaagagagc acctcgggg accgatccg gaggaagaa 
      tccacggcc gaagtgacg aatttaacg gattataac 
      gaaatattc aaggggcga ggactgact cggacaccg 
      cctccgctc gaacaacct gagccgttt caaaggcct 
      gaacatgac tattcggac ccgactgaa cctcagatc 
      ttggccgaa attccaaca gacaatttc gaggctctc 
      tccctggac aacaacatc aactgttac tcaccaccg 
      cctgcgttc acttcttca cagtattcc cgttcaaag 
      tccaatctg gatcaggtc tatgtcagc aggctgacg 
      aagcaacaa gaccgcgaa aataacatt tccaatatc 
      aataagaat aaaaatcaa ttgaccact ccaaggtat 
      tatcttcag aggcagagc agttgtaaa gatgtgcct 
      gaaaatgcg cccaaagcg aacaacagt attattaaa 
      catagcgtc tctgatgcc aatatcagc aagagtagt 
      atatcgtca aggaactca tcgagaaag aattccttt 
      tcaagacct agtcggaac tctactagt gtacctaat 
      gtttcgaaa attcccagc gctaggaat agcgaaaaa 
      gtggcaccg gttaaaacc tacattaca ccgacccct 
      gaagatgtg tttgttagt tcgaaactt tcgccagat 
      agtccatcc gaagaagtg gagtttgtt ccgatgtcg 
      atggacaga ccggcagga ctggacttg gacgatttt 
      cttccgaaa aaccatcga acgcttggc ttcgagcaa 
      caactgcca gatatgtcc gaagctgaa gtgttgggg 
      gctctggtg aggggtcac gagccgatg atggcaatg 
      ttggtgact cgtcagagg agtttgaaa gtggttttg 
      gctcaattg aggagtaag gatattaag acagcgact 
      gagactgca gttgtgatg ggtgatttg gccgttttg 
      gtcgatttg ttaggagtt tttaattgt aaatatacg 
      ttgtggaat ttggatttg tgtgtgctg attttaccc 
      aagattcaa gaattgctt cagagtaaa tttgaaacg 
      tacgtgacg acgggttgt aacaccctc aagctcatc 
      ctgaagcat ttcgggcca gtgatcaag agcaacgtt 
      caatccccg gtgggcagc ttcggcgtc gacattcct 
      cgcgaggag cgttacaac aaatcggtc aagtgccac 
      gaagtcttg caaaacttg cggactttt ctcgtcaaa 
      aaacagtca atgcccggt tcagtgggc tccactttt 
      cgcgaaatc agtacttta attcagaac accttcgac 
      tgacgaaag cccaaactg actcaccag aaccggagt 
      gtttgagaa gtgtctcga aattttaac atgtgattg 
      cgtattgga agaaaagac tagaaattc cgtccagtt 
      tgctaggag tagaggact aacggttta tgatactta 
      acgaagtta ctaaaaggt agttttaga taatttatt 
      tcactaaag aagacttag acgacgacg agcggttga 
      tggtgcgat tttttcttg cctgtttta acatgtttt 
      gtgccattt tgttttatt tgtttctcc cgaacgagt 
      tctgatggc ggaattaac ctcaattta tgtcttttt 
      tacctacac tagtgctta ataaaccaa actattgta 
      acataattt ataataaaa aagtgatta ttaa