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Gene information
General information
TC014039 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4638622..4655300
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014039
    iBeetle screen iB_02239

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014039 RA
      MPCEHPSSS AMFSAVTFF LVLATVLLP TTAADASPQ 
      RGRQRKGAT FPSSPSRER KPNIVLILT DDQDVELGS 
      LNFMPKTAR AIRDQGTEF RHAYVTTPM CCPSRSSLL 
      TGMYVHNHH VYTNNDNCS STQWQATYE TRSFATYLS 
      NAGYRTGYF GKYLNKYNG SYVPPGWRE WGGLIMNSK 
      YYNYSINMN GKKIKHGFD YHKDYYPDL IANDSIAFL 
      RQSKKNSAH KPVMLTMSF PAPHGPEDS APQYSHLFF 
      NVTTHHTPS YDYAPNPDK QWILQVTQK MQPIHKQFT 
      DLLMTKRLQ TLQSVDAAV QLIVEELRA LGELDNTYI 
      IYTSDHGYH LGQFGLIKG KSFPFEFDV RIPFLVRGP 
      GVEPGTVVD DIVLNIDLA PTFLDIAGV EAPPHMDGR 
      SVLPLFLNS KRKRMRWPD TFLIESSGR RETPYLDAR 
      IKARRNSTT EAPLSTLAP GDYSMATST KFWESSTVT 
      TTSPKKVYE SGEDLALDD EDDDNEEED IDVDEDEID 
      GGYDVDDDI YVYSNEANV TNKNLIRGE DETVLEGRS 
      LPPANKLER LTLECAADE MKYPCRRNQ KWYCINENG 
      RWRKHKCKT LHVPLAGNK LLRKCACFT QEGLVYKTM 
      FDRNITRRH TRIKRDTDL THILFTLID PDGMTSRAK 
      RDTLDHVET VLKDVQGKI IDLKSVNTS LMQPNLVTN 
      TISETSAGC IIMSKGRVN CSTSVYQDP KSWRQSRHQ 
      IDNEIYQLK QKLEKLKEI RRHLKHTRP LMGQADDEE 
      DNQKNKTAK FESPDVNEL NLHLGQEPK RKRKPVKIN 
      RIELTTSSP YFGHGNHHR HKHPDNDVT TSPPTRRPS 
      TTTKTPEDY ENCHCTPDP RPTKDPKHK IKEERLRKK 
      LRKQRKRDQ LEKECASER MNCFRHDKE HWKTAPMWT 
      EGPFCFCMN ANNNTYSCV RTINSTHNF LYCEFTTGL 
      VTFYNLRID PYELQNRIE QLKPEEKLF LHEQLKHLM 
      ACKGKACTV NHNAHQHHT KIRANVAPV VGAPHRYKK 
      RKLVEEGRG AAATHGSTP GVRISSTRE RGRNSSSMM 
      GILREAGIR RKRRKT
    CDS sequence
    • >TC014039 RA
      atgccatgt gagcaccct tccagcagt gccatgttc 
      agcgcggtg acttttttt ctcgtatta gcgacggtt 
      ttgttgccg acgaccgcg gctgatgcg tcccctcaa 
      cgaggcaga caacgaaaa ggtgccact ttcccatca 
      tcgccgtcg agagaacgc aaacccaac atcgtgctt 
      atcctcacc gacgatcag gacgtcgaa ctgggatcg 
      ttgaatttc atgccaaag acagcgagg gctatacga 
      gaccaaggg acagagttc aggcatgcc tacgttacg 
      acacccatg tgctgcccc tccaggtcc tcgctgctg 
      acggggatg tacgtccac aaccaccac gtctacacc 
      aacaacgac aactgctcc agcacccag tggcaggcc 
      acctatgag acgcggtcc tttgccaca tacttgtcc 
      aacgcggga tacaggaca ggctatttc ggaaaatat 
      ttgaacaaa tacaatgga tcgtacgtg ccgccaggt 
      tggcgcgaa tggggtggt ttaataatg aattcgaag 
      tattacaat tactcgatc aatatgaat gggaaaaag 
      atcaagcac ggcttcgat taccacaag gactactac 
      cccgacctg atcgccaac gactcgatc gccttcctt 
      cgccaatcg aagaaaaac agcgctcac aagcccgtc 
      atgctaacg atgtcgttt cctgcaccg cacgggccc 
      gaggactca gccccgcaa tactcgcac ctcttcttc 
      aacgtcacc acacatcat accccttcg tatgactac 
      gcgccaaac ccggacaag cagtggatc ctgcaagtg 
      acccagaag atgcaaccc atccacaaa caattcact 
      gatcttttg atgaccaaa cgtttgcag acgctccag 
      tctgtggac gccgccgtc cagctcatc gtcgaagag 
      ctgcgagct ttaggcgaa ctcgacaac acctacatc 
      atctacacg tcggaccat ggataccat ctcggacag 
      tttgggctg atcaagggc aaaagtttt ccgtttgaa 
      tttgatgtc cgaataccg tttctggtc cgcgggcca 
      ggtgtggaa cctggcacc gtggtcgac gatatagta 
      cttaacata gatctggca ccgacgttt ctggacatt 
      gccggcgtg gaggcgccc ccacacatg gacggcagg 
      tcggtgctt cccctcttt ttgaactcg aagaggaag 
      aggatgcga tggccggac acgttcctc attgaaagt 
      tctggacgt cgggaaacg ccgtacctc gacgctcgg 
      attaaggcc aggaggaac tccactacg gaagccccc 
      ttgtcgact ttagccccc ggggattat tctatggcc 
      acgtcgacg aaattctgg gaaagtagt accgtgaca 
      acgaccagt ccgaagaaa gtctacgag tcaggtgag 
      gatctggcg cttgatgac gaagatgac gacaatgag 
      gaggaagat attgacgtc gatgaagac gagattgat 
      ggtggttac gatgtcgat gacgatatt tacgtttac 
      agtaacgag gcaaatgtt actaataag aatctaatc 
      cgaggtgaa gacgagacg gttctagaa gggaggagt 
      ttaccaccg gccaataaa ttagaaaga ttgacgctg 
      gaatgtgcc gccgatgaa atgaaatat ccttgtcgg 
      aggaaccag aagtggtat tgcattaac gaaaacggg 
      aggtggagg aaacacaaa tgtaaaaca ctgcatgtg 
      cccttagcc ggaaacaag ttgttgcgg aagtgtgct 
      tgtttcact caggaggga ttggtttac aagaccatg 
      tttgatcga aacataaca cggcgtcat acgcgcatc 
      aaacgtgac actgacctc acccacatc ctcttcact 
      ctaatagac ccagacgga atgacctcg cgagcaaaa 
      cgtgacact ctcgatcac gtcgaaacc gtgctgaaa 
      gacgtccaa ggcaaaata atcgacttg aaatcagtc 
      aacacatct ctgatgcaa cccaacttg gtcaccaac 
      acgatttcg gaaacctca gccggttgt ataatcatg 
      agcaaaggt cgggtcaac tgttccacc tcggtgtat 
      caagacccg aaatcttgg agacaatca cgccatcag 
      atagacaac gaaatctac caactgaag caaaagctc 
      gaaaaactg aaagaaata cgccgccat ttgaagcac 
      acgcgccct ctaatgggg caagctgac gacgaagag 
      gacaatcag aaaaacaaa actgcaaaa ttcgaatct 
      ccggacgtg aacgaacta aacttgcac ctaggacaa 
      gaaccgaaa cgaaaacgc aaaccggtg aaaatcaac 
      cgaattgag ctaacaacc agtagcccc tacttcggt 
      cacggcaac caccaccgt cacaagcac ccagataat 
      gacgtcaca acgtcgcct ccaacccgg agaccttcc 
      accacgacc aaaaccccc gaagattac gaaaactgc 
      cactgcacc cccgacccc aggcccacc aaagacccc 
      aaacacaaa atcaaggag gaacgacta cgtaaaaag 
      ttgcgtaag cagcgtaaa cgcgaccaa ttggagaaa 
      gagtgtgcg tctgaacgc atgaattgc ttccgtcat 
      gacaaggag cactggaag accgctccg atgtggacc 
      gagggcccc ttttgtttt tgtatgaat gccaacaac 
      aacacttat tcctgtgtt cggacgatc aattcaacg 
      cacaatttc ctctactgt gaattcacc accggtttg 
      gtgactttt tataatctg aggatcgat ccgtacgag 
      ttgcagaat cggatcgag cagttgaag ccggaagaa 
      aagttgttt cttcacgaa cagttgaag catctgatg 
      gcctgtaag ggcaaggca tgcaccgtt aatcacaac 
      gcgcatcag caccacacc aaaattagg gccaatgtg 
      gcgccggtt gtgggggcg ccacataga tacaagaag 
      aggaagcta gtcgaagag ggacgtggc gccgccgca 
      acccatgga agtacacct ggcgtcaga ataagcagc 
      acgagagaa cgcggcaga aacagttcg tctatgatg 
      ggaatttta agagaagct ggcattagg cggaaacgg 
      aggaaaacg tga
    mRNA sequence
    • >TC014039 RA
      accgaaaag tatccaaag taaacgata gagagtcgc 
      tttagttca caccgtttt ggctccgcg tccttccca 
      agggttctt ggcgacgta aaaaccggt cgtgtcttt 
      gtttgccca ccctgtatg gtattgtcg cgtcctcgt 
      ccaaatctt ccacttcgc cgtgggtcg agtaaaatc 
      gcggaaatt gattaaatc aattttggg ctcgcacaa 
      aacgacacc aattgcgac aataactcc tccaaaggc 
      gttcattaa tcatatttt cgggaagtt tagacaagc 
      gactccatc catctatcc atctcgagg agtagtccg 
      ggccggcac caaacggaa tcgtttaac cactcacca 
      gagtttaac ttcgctgct tgactgctt ctcagttta 
      cttttaacc ttagagcga ggtggtgca tgcgatcgc 
      ttcgccgat tcctatcgc gtgtttcca tcttttcgg 
      atttttggg gtggctttc gacgaaatt ccgccgaaa 
      attcaagat gatttagcg agttcgcat gccatgtga 
      gcacccttc cagcagtgc catgttcag cgcggtgac 
      tttttttct cgtattagc gacggtttt gttgccgac 
      gaccgcggc tgatgcgtc ccctcaacg aggcagaca 
      acgaaaagg tgccacttt cccatcatc gccgtcgag 
      agaacgcaa acccaacat cgtgcttat cctcaccga 
      cgatcagga cgtcgaact gggatcgtt gaatttcat 
      gccaaagac agcgagggc tatacgaga ccaagggac 
      agagttcag gcatgccta cgttacgac acccatgtg 
      ctgcccctc caggtcctc gctgctgac ggggatgta 
      cgtccacaa ccaccacgt ctacaccaa caacgacaa 
      ctgctccag cacccagtg gcaggccac ctatgagac 
      gcggtcctt tgccacata cttgtccaa cgcgggata 
      caggacagg ctatttcgg aaaatattt gaacaaata 
      caatggatc gtacgtgcc gccaggttg gcgcgaatg 
      gggtggttt aataatgaa ttcgaagta ttacaatta 
      ctcgatcaa tatgaatgg gaaaaagat caagcacgg 
      cttcgatta ccacaagga ctactaccc cgacctgat 
      cgccaacga ctcgatcgc cttccttcg ccaatcgaa 
      gaaaaacag cgctcacaa gcccgtcat gctaacgat 
      gtcgtttcc tgcaccgca cgggcccga ggactcagc 
      cccgcaata ctcgcacct cttcttcaa cgtcaccac 
      acatcatac cccttcgta tgactacgc gccaaaccc 
      ggacaagca gtggatcct gcaagtgac ccagaagat 
      gcaacccat ccacaaaca attcactga tcttttgat 
      gaccaaacg tttgcagac gctccagtc tgtggacgc 
      cgccgtcca gctcatcgt cgaagagct gcgagcttt 
      aggcgaact cgacaacac ctacatcat ctacacgtc 
      ggaccatgg ataccatct cggacagtt tgggctgat 
      caagggcaa aagttttcc gtttgaatt tgatgtccg 
      aataccgtt tctggtccg cgggccagg tgtggaacc 
      tggcaccgt ggtcgacga tatagtact taacataga 
      tctggcacc gacgtttct ggacattgc cggcgtgga 
      ggcgccccc acacatgga cggcaggtc ggtgcttcc 
      cctcttttt gaactcgaa gaggaagag gatgcgatg 
      gccggacac gttcctcat tgaaagttc tggacgtcg 
      ggaaacgcc gtacctcga cgctcggat taaggccag 
      gaggaactc cactacgga agccccctt gtcgacttt 
      agcccccgg ggattattc tatggccac gtcgacgaa 
      attctggga aagtagtac cgtgacaac gaccagtcc 
      gaagaaagt ctacgagtc aggtgagga tctggcgct 
      tgatgacga agatgacga caatgagga ggaagatat 
      tgacgtcga tgaagacga gattgatgg tggttacga 
      tgtcgatga cgatattta cgtttacag taacgaggc 
      aaatgttac taataagaa tctaatccg aggtgaaga 
      cgagacggt tctagaagg gaggagttt accaccggc 
      caataaatt agaaagatt gacgctgga atgtgccgc 
      cgatgaaat gaaatatcc ttgtcggag gaaccagaa 
      gtggtattg cattaacga aaacgggag gtggaggaa 
      acacaaatg taaaacact gcatgtgcc cttagccgg 
      aaacaagtt gttgcggaa gtgtgcttg tttcactca 
      ggagggatt ggtttacaa gaccatgtt tgatcgaaa 
      cataacacg gcgtcatac gcgcatcaa acgtgacac 
      tgacctcac ccacatcct cttcactct aatagaccc 
      agacggaat gacctcgcg agcaaaacg tgacactct 
      cgatcacgt cgaaaccgt gctgaaaga cgtccaagg 
      caaaataat cgacttgaa atcagtcaa cacatctct 
      gatgcaacc caacttggt caccaacac gatttcgga 
      aacctcagc cggttgtat aatcatgag caaaggtcg 
      ggtcaactg ttccacctc ggtgtatca agacccgaa 
      atcttggag acaatcacg ccatcagat agacaacga 
      aatctacca actgaagca aaagctcga aaaactgaa 
      agaaatacg ccgccattt gaagcacac gcgccctct 
      aatggggca agctgacga cgaagagga caatcagaa 
      aaacaaaac tgcaaaatt cgaatctcc ggacgtgaa 
      cgaactaaa cttgcacct aggacaaga accgaaacg 
      aaaacgcaa accggtgaa aatcaaccg aattgagct 
      aacaaccag tagccccta cttcggtca cggcaacca 
      ccaccgtca caagcaccc agataatga cgtcacaac 
      gtcgcctcc aacccggag accttccac cacgaccaa 
      aacccccga agattacga aaactgcca ctgcacccc 
      cgaccccag gcccaccaa agaccccaa acacaaaat 
      caaggagga acgactacg taaaaagtt gcgtaagca 
      gcgtaaacg cgaccaatt ggagaaaga gtgtgcgtc 
      tgaacgcat gaattgctt ccgtcatga caaggagca 
      ctggaagac cgctccgat gtggaccga gggcccctt 
      ttgtttttg tatgaatgc caacaacaa cacttattc 
      ctgtgttcg gacgatcaa ttcaacgca caatttcct 
      ctactgtga attcaccac cggtttggt gacttttta 
      taatctgag gatcgatcc gtacgagtt gcagaatcg 
      gatcgagca gttgaagcc ggaagaaaa gttgtttct 
      tcacgaaca gttgaagca tctgatggc ctgtaaggg 
      caaggcatg caccgttaa tcacaacgc gcatcagca 
      ccacaccaa aattagggc caatgtggc gccggttgt 
      gggggcgcc acatagata caagaagag gaagctagt 
      cgaagaggg acgtggcgc cgccgcaac ccatggaag 
      tacacctgg cgtcagaat aagcagcac gagagaacg 
      cggcagaaa cagttcgtc tatgatggg aattttaag 
      agaagctgg cattaggcg gaaacggag gaaaacgtg 
      aacactatc aataagaat aatcgactg aatttaaag 
      aagtatatc atttaatga tgcatttat tgtgatctc 
      taatttatg atttcgttt gtaaattaa tttgtaggt 
      ttcgaagct tatgcttta tatgtaatt gtgtaaaaa 
      ctatagtaa ggtaatgtt aggtacctg catacttcc 
      actaaacaa actagcaat atctctttc ggaatttct 
      atttaataa agtgaaacg aagggcaat aagttattt 
      ttgtaacgc gttactttt gttcactct tgacttgta 
      tttctgtct agtccaaat cgttttgta tataggtaa 
      tccttgtat tgttataac ctgcttggt acaattatt 
      tcaatcaat tccaaacaa tacacgtgt aacaaggac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY