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Gene information
General information
TC014026 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4428310..4432161
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014026
    iBeetle screen iB_02238

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     larva segmentation/division incomplete - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 77.78% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection
    Comment: nearly completely eaten up

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    Pupal morphology
    head slightlyorientation irregular - Number of animals showing the phenotype: 3

    antenna slightlyorientation irregular - Number of animals showing the phenotype: 2

    wings not closed - Number of animals showing the phenotype: 3

    wings shortened - Number of animals showing the phenotype: 2
    Adult morphology
    wings not closed - Number of animals showing the phenotype: 1


    prosternum anterior rim irregular - Number of animals showing the phenotype: 1

    prosternum anterior shape irregular - Number of animals showing the phenotype: 1

    mesobasisternum posterior dv-orientation irregular - Number of animals showing the phenotype: 1

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014026 RA
      MGGLFSSQG NDYSDDSCS EEESNEVSC KNQPTFDED 
NCLDSTDTI DASLSQPAM PITLNVDQV MSIENDLDD 
YEKVALVFL IYEDPKSAF VQLTKLLQG SHDKLLYNW 
AIHENLAGR KWQEKLVEA LCIIQNYQI ISSLGFKRE 
DLTARFLPH HIFTYAYVN KVRKALYFV CEALQQKDV 
ENLLNLACK DFREKGLDF KCINAQFLE LYLLHWETV 
GYVKLDDLS NLCRVLKQI DQLDICDKL REIVPIVHK 
SAESRSVDK RVDSSESEK RQSVSVFEG ILDPKSSLL 
SLASLETSH ISADNAAFV SDENKYFID PERPGICLI 
INQENFYRE VDRKYRHLL PRRDNKLEL RIGTQYDRD 
KLEATFRQF GFKTIVENN LTHLEMLRK IEEVVQSVN 
DESSLFISI MSHGDVGVV YGVNSCQVD VKAIKRVMC 
TKNRPHLSG KPKVLILQS CQGTRCQKI QSDDDDIET 
DGPSSPVQE SVPPIADLF TFWATVPGY GAIRNKKTG 
SWFIQSLCG KMKELGHKY HFDDICTSV IGDVSRKKW 
CQEKDEVAM VPEKESTFR KLFYLPPVR V
    CDS sequence
    • >TC014026 RA
      atgggtgga ttgttttca agccaagga aatgattat 
tccgacgat tcgtgttcc gaagaggaa agcaacgag 
gtgtcgtgc aaaaatcaa cctaccttt gatgaggac 
aattgtttg gactccact gacaccata gacgccagt 
ttatcgcaa ccagcgatg ccaataacg ctaaatgtg 
gaccaagtt atgtccatt gaaaacgac ttggatgac 
tacgagaag gtggccctt gtgtttttg atttacgaa 
gaccccaaa tctgcgttt gttcaacta acgaaattg 
ctccaaggg tcccatgac aaactcttg tataattgg 
gcaattcat gagaactta gccggtcgg aagtggcaa 
gaaaagctg gttgaggca ctttgcatc atccagaat 
taccaaatc atatcaagt ttgggcttc aaacgggag 
gatttaacc gctcggttt ttaccgcac cacattttc 
acgtacgcc tatgtgaac aaagtcaga aaggccctc 
tatttcgtc tgcgaggcg ttgcagcag aaggacgtt 
gagaatttg ctcaacttg gcgtgtaag gacttcagg 
gagaagggg ctggatttt aagtgcatt aatgcgcaa 
tttttggag ttgtatttg ctgcattgg gagactgtg 
gggtacgta aaactggac gatttgagt aatttgtgt 
agagttctc aagcaaatt gaccagttg gatatttgt 
gataaactg agagaaatt gtgccaatt gtgcacaaa 
tctgctgag agtagaagt gtagataaa agagtagat 
tctagtgag agtgaaaaa agacagtca gtgagtgtg 
tttgaggga attttagat ccaaagtcc agtttgctt 
agtctggcc tcgctggag acgtcgcat atcagtgca 
gacaatgca gcttttgtc agcgatgaa aataaatat 
tttattgat ccagaaaga cctggcatt tgtttgatc 
atcaatcaa gagaatttc tacagagaa gtggacaga 
aaatatcga cacttgctt ccacgccgg gacaacaaa 
ttagaactt aggattggt acacaatac gaccgagac 
aagcttgaa gccactttc cgacaattt ggtttcaaa 
actattgta gaaaataat ctcactcat ttggaaatg 
ctacgaaaa atagaagaa gtagtgcaa agcgttaac 
gatgagtcg agccttttc atttctata atgtctcat 
ggtgatgta ggtgtcgtt tacggcgtt aatagttgc 
caagtcgac gtcaaagca attaaacgt gtgatgtgt 
accaaaaat cgcccacat ttatcgggg aaacccaaa 
gttttaatc ctccagtct tgtcaaggc accagatgt 
caaaaaatt cagtcagat gacgatgac attgaaaca 
gacggtcct tcgtcacct gtccaagaa tcagtcccg 
ccaatagca gatttgttc actttctgg gccacagtt 
ccaggctat ggtgcaata aggaacaaa aaaacagga 
tcctggttt attcagtcc ttgtgcgga aaaatgaaa 
gaattgggg cataagtac catttcgat gatatctgc 
accagtgtt ataggggac gtaagtagg aaaaaatgg 
tgccaagag aaggacgaa gtggcaatg gtgccagaa 
aaggagtcc acattcagg aaactgttt tatctgcca 
cccgttaga gtgtaa
    mRNA sequence
    • >TC014026 RA
      tcacgggga agtcccttg ttgagttac gtgttttaa 
tcagtttat cgaattaat tgttattta aaaccaacg 
acaatgggt ggattgttt tcaagccaa ggaaatgat 
tattccgac gattcgtgt tccgaagag gaaagcaac 
gaggtgtcg tgcaaaaat caacctacc tttgatgag 
gacaattgt ttggactcc actgacacc atagacgcc 
agtttatcg caaccagcg atgccaata acgctaaat 
gtggaccaa gttatgtcc attgaaaac gacttggat 
gactacgag aaggtggcc cttgtgttt ttgatttac 
gaagacccc aaatctgcg tttgttcaa ctaacgaaa 
ttgctccaa gggtcccat gacaaactc ttgtataat 
tgggcaatt catgagaac ttagccggt cggaagtgg 
caagaaaag ctggttgag gcactttgc atcatccag 
aattaccaa atcatatca agtttgggc ttcaaacgg 
gaggattta accgctcgg tttttaccg caccacatt 
ttcacgtac gcctatgtg aacaaagtc agaaaggcc 
ctctatttc gtctgcgag gcgttgcag cagaaggac 
gttgagaat ttgctcaac ttggcgtgt aaggacttc 
agggagaag gggctggat tttaagtgc attaatgcg 
caatttttg gagttgtat ttgctgcat tgggagact 
gtggggtac gtaaaactg gacgatttg agtaatttg 
tgtagagtt ctcaagcaa attgaccag ttggatatt 
tgtgataaa ctgagagaa attgtgcca attgtgcac 
aaatctgct gagagtaga agtgtagat aaaagagta 
gattctagt gagagtgaa aaaagacag tcagtgagt 
gtgtttgag ggaatttta gatccaaag tccagtttg 
cttagtctg gcctcgctg gagacgtcg catatcagt 
gcagacaat gcagctttt gtcagcgat gaaaataaa 
tattttatt gatccagaa agacctggc atttgtttg 
atcatcaat caagagaat ttctacaga gaagtggac 
agaaaatat cgacacttg cttccacgc cgggacaac 
aaattagaa cttaggatt ggtacacaa tacgaccga 
gacaagctt gaagccact ttccgacaa tttggtttc 
aaaactatt gtagaaaat aatctcact catttggaa 
atgctacga aaaatagaa gaagtagtg caaagcgtt 
aacgatgag tcgagcctt ttcatttct ataatgtct 
catggtgat gtaggtgtc gtttacggc gttaatagt 
tgccaagtc gacgtcaaa gcaattaaa cgtgtgatg 
tgtaccaaa aatcgccca catttatcg gggaaaccc 
aaagtttta atcctccag tcttgtcaa ggcaccaga 
tgtcaaaaa attcagtca gatgacgat gacattgaa 
acagacggt ccttcgtca cctgtccaa gaatcagtc 
ccgccaata gcagatttg ttcactttc tgggccaca 
gttccaggc tatggtgca ataaggaac aaaaaaaca 
ggatcctgg tttattcag tccttgtgc ggaaaaatg 
aaagaattg gggcataag taccatttc gatgatatc 
tgcaccagt gttataggg gacgtaagt aggaaaaaa 
tggtgccaa gagaaggac gaagtggca atggtgcca 
gaaaaggag tccacattc aggaaactg ttttatctg 
ccacccgtt agagtgtaa gtttttaaa aaattatta 
agcaatatg atttttaaa ttaagttgg tgacactga 
aaaactgaa aaatgttgc taacttaat ttcaattat 
atttaattt ttttaatga aaactcaaa taatttttg 
ttcaaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY