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Gene information
General information
TC014026 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4428310..4432161
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014026
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02238

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    larva segmentation/division incomplete - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 77.78% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    Comment: nearly completely eaten up

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    head slightlyorientation irregular - Number of animals showing the phenotype: 3

    antenna slightlyorientation irregular - Number of animals showing the phenotype: 2

    wings not closed - Number of animals showing the phenotype: 3

    wings shortened - Number of animals showing the phenotype: 2
    Adult morphology
    wings not closed - Number of animals showing the phenotype: 1


    prosternum anterior rim irregular - Number of animals showing the phenotype: 1

    prosternum anterior shape irregular - Number of animals showing the phenotype: 1

    mesobasisternum posterior dv-orientation irregular - Number of animals showing the phenotype: 1

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014026 RA
      MGGLFSSQG NDYSDDSCS EEESNEVSC KNQPTFDED 
      NCLDSTDTI DASLSQPAM PITLNVDQV MSIENDLDD 
      YEKVALVFL IYEDPKSAF VQLTKLLQG SHDKLLYNW 
      AIHENLAGR KWQEKLVEA LCIIQNYQI ISSLGFKRE 
      DLTARFLPH HIFTYAYVN KVRKALYFV CEALQQKDV 
      ENLLNLACK DFREKGLDF KCINAQFLE LYLLHWETV 
      GYVKLDDLS NLCRVLKQI DQLDICDKL REIVPIVHK 
      SAESRSVDK RVDSSESEK RQSVSVFEG ILDPKSSLL 
      SLASLETSH ISADNAAFV SDENKYFID PERPGICLI 
      INQENFYRE VDRKYRHLL PRRDNKLEL RIGTQYDRD 
      KLEATFRQF GFKTIVENN LTHLEMLRK IEEVVQSVN 
      DESSLFISI MSHGDVGVV YGVNSCQVD VKAIKRVMC 
      TKNRPHLSG KPKVLILQS CQGTRCQKI QSDDDDIET 
      DGPSSPVQE SVPPIADLF TFWATVPGY GAIRNKKTG 
      SWFIQSLCG KMKELGHKY HFDDICTSV IGDVSRKKW 
      CQEKDEVAM VPEKESTFR KLFYLPPVR V
    CDS sequence
    • >TC014026 RA
      atgggtgga ttgttttca agccaagga aatgattat 
      tccgacgat tcgtgttcc gaagaggaa agcaacgag 
      gtgtcgtgc aaaaatcaa cctaccttt gatgaggac 
      aattgtttg gactccact gacaccata gacgccagt 
      ttatcgcaa ccagcgatg ccaataacg ctaaatgtg 
      gaccaagtt atgtccatt gaaaacgac ttggatgac 
      tacgagaag gtggccctt gtgtttttg atttacgaa 
      gaccccaaa tctgcgttt gttcaacta acgaaattg 
      ctccaaggg tcccatgac aaactcttg tataattgg 
      gcaattcat gagaactta gccggtcgg aagtggcaa 
      gaaaagctg gttgaggca ctttgcatc atccagaat 
      taccaaatc atatcaagt ttgggcttc aaacgggag 
      gatttaacc gctcggttt ttaccgcac cacattttc 
      acgtacgcc tatgtgaac aaagtcaga aaggccctc 
      tatttcgtc tgcgaggcg ttgcagcag aaggacgtt 
      gagaatttg ctcaacttg gcgtgtaag gacttcagg 
      gagaagggg ctggatttt aagtgcatt aatgcgcaa 
      tttttggag ttgtatttg ctgcattgg gagactgtg 
      gggtacgta aaactggac gatttgagt aatttgtgt 
      agagttctc aagcaaatt gaccagttg gatatttgt 
      gataaactg agagaaatt gtgccaatt gtgcacaaa 
      tctgctgag agtagaagt gtagataaa agagtagat 
      tctagtgag agtgaaaaa agacagtca gtgagtgtg 
      tttgaggga attttagat ccaaagtcc agtttgctt 
      agtctggcc tcgctggag acgtcgcat atcagtgca 
      gacaatgca gcttttgtc agcgatgaa aataaatat 
      tttattgat ccagaaaga cctggcatt tgtttgatc 
      atcaatcaa gagaatttc tacagagaa gtggacaga 
      aaatatcga cacttgctt ccacgccgg gacaacaaa 
      ttagaactt aggattggt acacaatac gaccgagac 
      aagcttgaa gccactttc cgacaattt ggtttcaaa 
      actattgta gaaaataat ctcactcat ttggaaatg 
      ctacgaaaa atagaagaa gtagtgcaa agcgttaac 
      gatgagtcg agccttttc atttctata atgtctcat 
      ggtgatgta ggtgtcgtt tacggcgtt aatagttgc 
      caagtcgac gtcaaagca attaaacgt gtgatgtgt 
      accaaaaat cgcccacat ttatcgggg aaacccaaa 
      gttttaatc ctccagtct tgtcaaggc accagatgt 
      caaaaaatt cagtcagat gacgatgac attgaaaca 
      gacggtcct tcgtcacct gtccaagaa tcagtcccg 
      ccaatagca gatttgttc actttctgg gccacagtt 
      ccaggctat ggtgcaata aggaacaaa aaaacagga 
      tcctggttt attcagtcc ttgtgcgga aaaatgaaa 
      gaattgggg cataagtac catttcgat gatatctgc 
      accagtgtt ataggggac gtaagtagg aaaaaatgg 
      tgccaagag aaggacgaa gtggcaatg gtgccagaa 
      aaggagtcc acattcagg aaactgttt tatctgcca 
      cccgttaga gtgtaa
    mRNA sequence
    • >TC014026 RA
      tcacgggga agtcccttg ttgagttac gtgttttaa 
      tcagtttat cgaattaat tgttattta aaaccaacg 
      acaatgggt ggattgttt tcaagccaa ggaaatgat 
      tattccgac gattcgtgt tccgaagag gaaagcaac 
      gaggtgtcg tgcaaaaat caacctacc tttgatgag 
      gacaattgt ttggactcc actgacacc atagacgcc 
      agtttatcg caaccagcg atgccaata acgctaaat 
      gtggaccaa gttatgtcc attgaaaac gacttggat 
      gactacgag aaggtggcc cttgtgttt ttgatttac 
      gaagacccc aaatctgcg tttgttcaa ctaacgaaa 
      ttgctccaa gggtcccat gacaaactc ttgtataat 
      tgggcaatt catgagaac ttagccggt cggaagtgg 
      caagaaaag ctggttgag gcactttgc atcatccag 
      aattaccaa atcatatca agtttgggc ttcaaacgg 
      gaggattta accgctcgg tttttaccg caccacatt 
      ttcacgtac gcctatgtg aacaaagtc agaaaggcc 
      ctctatttc gtctgcgag gcgttgcag cagaaggac 
      gttgagaat ttgctcaac ttggcgtgt aaggacttc 
      agggagaag gggctggat tttaagtgc attaatgcg 
      caatttttg gagttgtat ttgctgcat tgggagact 
      gtggggtac gtaaaactg gacgatttg agtaatttg 
      tgtagagtt ctcaagcaa attgaccag ttggatatt 
      tgtgataaa ctgagagaa attgtgcca attgtgcac 
      aaatctgct gagagtaga agtgtagat aaaagagta 
      gattctagt gagagtgaa aaaagacag tcagtgagt 
      gtgtttgag ggaatttta gatccaaag tccagtttg 
      cttagtctg gcctcgctg gagacgtcg catatcagt 
      gcagacaat gcagctttt gtcagcgat gaaaataaa 
      tattttatt gatccagaa agacctggc atttgtttg 
      atcatcaat caagagaat ttctacaga gaagtggac 
      agaaaatat cgacacttg cttccacgc cgggacaac 
      aaattagaa cttaggatt ggtacacaa tacgaccga 
      gacaagctt gaagccact ttccgacaa tttggtttc 
      aaaactatt gtagaaaat aatctcact catttggaa 
      atgctacga aaaatagaa gaagtagtg caaagcgtt 
      aacgatgag tcgagcctt ttcatttct ataatgtct 
      catggtgat gtaggtgtc gtttacggc gttaatagt 
      tgccaagtc gacgtcaaa gcaattaaa cgtgtgatg 
      tgtaccaaa aatcgccca catttatcg gggaaaccc 
      aaagtttta atcctccag tcttgtcaa ggcaccaga 
      tgtcaaaaa attcagtca gatgacgat gacattgaa 
      acagacggt ccttcgtca cctgtccaa gaatcagtc 
      ccgccaata gcagatttg ttcactttc tgggccaca 
      gttccaggc tatggtgca ataaggaac aaaaaaaca 
      ggatcctgg tttattcag tccttgtgc ggaaaaatg 
      aaagaattg gggcataag taccatttc gatgatatc 
      tgcaccagt gttataggg gacgtaagt aggaaaaaa 
      tggtgccaa gagaaggac gaagtggca atggtgcca 
      gaaaaggag tccacattc aggaaactg ttttatctg 
      ccacccgtt agagtgtaa gtttttaaa aaattatta 
      agcaatatg atttttaaa ttaagttgg tgacactga 
      aaaactgaa aaatgttgc taacttaat ttcaattat 
      atttaattt ttttaatga aaactcaaa taatttttg 
      ttcaaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY