General information | |||
TC014026 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 4428310..4432161 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC014026 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02238 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
larva segmentation/division incomplete - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 77.78% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
Comment: nearly completely eaten up |
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (19 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (22 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (41 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (41 dpi)
antenna slightlyorientation irregular - Number of animals showing the phenotype: 2
wings not closed - Number of animals showing the phenotype: 3
wings shortened - Number of animals showing the phenotype: 2
prosternum anterior rim irregular - Number of animals showing the phenotype: 1
prosternum anterior shape irregular - Number of animals showing the phenotype: 1
mesobasisternum posterior dv-orientation irregular - Number of animals showing the phenotype: 1
- >TC014026 RA
MGGLFSSQG NDYSDDSCS EEESNEVSC KNQPTFDED NCLDSTDTI DASLSQPAM PITLNVDQV MSIENDLDD YEKVALVFL IYEDPKSAF VQLTKLLQG SHDKLLYNW AIHENLAGR KWQEKLVEA LCIIQNYQI ISSLGFKRE DLTARFLPH HIFTYAYVN KVRKALYFV CEALQQKDV ENLLNLACK DFREKGLDF KCINAQFLE LYLLHWETV GYVKLDDLS NLCRVLKQI DQLDICDKL REIVPIVHK SAESRSVDK RVDSSESEK RQSVSVFEG ILDPKSSLL SLASLETSH ISADNAAFV SDENKYFID PERPGICLI INQENFYRE VDRKYRHLL PRRDNKLEL RIGTQYDRD KLEATFRQF GFKTIVENN LTHLEMLRK IEEVVQSVN DESSLFISI MSHGDVGVV YGVNSCQVD VKAIKRVMC TKNRPHLSG KPKVLILQS CQGTRCQKI QSDDDDIET DGPSSPVQE SVPPIADLF TFWATVPGY GAIRNKKTG SWFIQSLCG KMKELGHKY HFDDICTSV IGDVSRKKW CQEKDEVAM VPEKESTFR KLFYLPPVR V
- >TC014026 RA
atgggtgga ttgttttca agccaagga aatgattat tccgacgat tcgtgttcc gaagaggaa agcaacgag gtgtcgtgc aaaaatcaa cctaccttt gatgaggac aattgtttg gactccact gacaccata gacgccagt ttatcgcaa ccagcgatg ccaataacg ctaaatgtg gaccaagtt atgtccatt gaaaacgac ttggatgac tacgagaag gtggccctt gtgtttttg atttacgaa gaccccaaa tctgcgttt gttcaacta acgaaattg ctccaaggg tcccatgac aaactcttg tataattgg gcaattcat gagaactta gccggtcgg aagtggcaa gaaaagctg gttgaggca ctttgcatc atccagaat taccaaatc atatcaagt ttgggcttc aaacgggag gatttaacc gctcggttt ttaccgcac cacattttc acgtacgcc tatgtgaac aaagtcaga aaggccctc tatttcgtc tgcgaggcg ttgcagcag aaggacgtt gagaatttg ctcaacttg gcgtgtaag gacttcagg gagaagggg ctggatttt aagtgcatt aatgcgcaa tttttggag ttgtatttg ctgcattgg gagactgtg gggtacgta aaactggac gatttgagt aatttgtgt agagttctc aagcaaatt gaccagttg gatatttgt gataaactg agagaaatt gtgccaatt gtgcacaaa tctgctgag agtagaagt gtagataaa agagtagat tctagtgag agtgaaaaa agacagtca gtgagtgtg tttgaggga attttagat ccaaagtcc agtttgctt agtctggcc tcgctggag acgtcgcat atcagtgca gacaatgca gcttttgtc agcgatgaa aataaatat tttattgat ccagaaaga cctggcatt tgtttgatc atcaatcaa gagaatttc tacagagaa gtggacaga aaatatcga cacttgctt ccacgccgg gacaacaaa ttagaactt aggattggt acacaatac gaccgagac aagcttgaa gccactttc cgacaattt ggtttcaaa actattgta gaaaataat ctcactcat ttggaaatg ctacgaaaa atagaagaa gtagtgcaa agcgttaac gatgagtcg agccttttc atttctata atgtctcat ggtgatgta ggtgtcgtt tacggcgtt aatagttgc caagtcgac gtcaaagca attaaacgt gtgatgtgt accaaaaat cgcccacat ttatcgggg aaacccaaa gttttaatc ctccagtct tgtcaaggc accagatgt caaaaaatt cagtcagat gacgatgac attgaaaca gacggtcct tcgtcacct gtccaagaa tcagtcccg ccaatagca gatttgttc actttctgg gccacagtt ccaggctat ggtgcaata aggaacaaa aaaacagga tcctggttt attcagtcc ttgtgcgga aaaatgaaa gaattgggg cataagtac catttcgat gatatctgc accagtgtt ataggggac gtaagtagg aaaaaatgg tgccaagag aaggacgaa gtggcaatg gtgccagaa aaggagtcc acattcagg aaactgttt tatctgcca cccgttaga gtgtaa
- >TC014026 RA
tcacgggga agtcccttg ttgagttac gtgttttaa tcagtttat cgaattaat tgttattta aaaccaacg acaatgggt ggattgttt tcaagccaa ggaaatgat tattccgac gattcgtgt tccgaagag gaaagcaac gaggtgtcg tgcaaaaat caacctacc tttgatgag gacaattgt ttggactcc actgacacc atagacgcc agtttatcg caaccagcg atgccaata acgctaaat gtggaccaa gttatgtcc attgaaaac gacttggat gactacgag aaggtggcc cttgtgttt ttgatttac gaagacccc aaatctgcg tttgttcaa ctaacgaaa ttgctccaa gggtcccat gacaaactc ttgtataat tgggcaatt catgagaac ttagccggt cggaagtgg caagaaaag ctggttgag gcactttgc atcatccag aattaccaa atcatatca agtttgggc ttcaaacgg gaggattta accgctcgg tttttaccg caccacatt ttcacgtac gcctatgtg aacaaagtc agaaaggcc ctctatttc gtctgcgag gcgttgcag cagaaggac gttgagaat ttgctcaac ttggcgtgt aaggacttc agggagaag gggctggat tttaagtgc attaatgcg caatttttg gagttgtat ttgctgcat tgggagact gtggggtac gtaaaactg gacgatttg agtaatttg tgtagagtt ctcaagcaa attgaccag ttggatatt tgtgataaa ctgagagaa attgtgcca attgtgcac aaatctgct gagagtaga agtgtagat aaaagagta gattctagt gagagtgaa aaaagacag tcagtgagt gtgtttgag ggaatttta gatccaaag tccagtttg cttagtctg gcctcgctg gagacgtcg catatcagt gcagacaat gcagctttt gtcagcgat gaaaataaa tattttatt gatccagaa agacctggc atttgtttg atcatcaat caagagaat ttctacaga gaagtggac agaaaatat cgacacttg cttccacgc cgggacaac aaattagaa cttaggatt ggtacacaa tacgaccga gacaagctt gaagccact ttccgacaa tttggtttc aaaactatt gtagaaaat aatctcact catttggaa atgctacga aaaatagaa gaagtagtg caaagcgtt aacgatgag tcgagcctt ttcatttct ataatgtct catggtgat gtaggtgtc gtttacggc gttaatagt tgccaagtc gacgtcaaa gcaattaaa cgtgtgatg tgtaccaaa aatcgccca catttatcg gggaaaccc aaagtttta atcctccag tcttgtcaa ggcaccaga tgtcaaaaa attcagtca gatgacgat gacattgaa acagacggt ccttcgtca cctgtccaa gaatcagtc ccgccaata gcagatttg ttcactttc tgggccaca gttccaggc tatggtgca ataaggaac aaaaaaaca ggatcctgg tttattcag tccttgtgc ggaaaaatg aaagaattg gggcataag taccatttc gatgatatc tgcaccagt gttataggg gacgtaagt aggaaaaaa tggtgccaa gagaaggac gaagtggca atggtgcca gaaaaggag tccacattc aggaaactg ttttatctg ccacccgtt agagtgtaa gtttttaaa aaattatta agcaatatg atttttaaa ttaagttgg tgacactga aaaactgaa aaatgttgc taacttaat ttcaattat atttaattt ttttaatga aaactcaaa taatttttg ttcaaaaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
