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Gene information
General information
TC014008 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4030741..4035864
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014008
    iBeetle screen iB_02235

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014008 RA
      MQIRKVFLL AISSAFGIL MLLIVTRDS PINDIDRKF 
      RLEKIDELI HSQASSLAC KQPNLPVFS PEMMKFVHE 
      EPPIDCSSA GPDWVKCQG SECRIQDEI KARHGRVTC 
      SFTDIIRVN DFESTSGET TYSDSYYKL EASDVVKVS 
      CRGQKAQWS ATLTGIRLD KKIWENTSW SKLPSDAVK 
      FNVLMFGFD SISRNSFVR KLPKSYKFL TEILHGDVL 
      QGYNIIGDG TPQALIPIL TGKTELELP DTRKRFANT 
      KFVNSYPFI WNEYKKAGY VTGYFEDVP ELGIFTYRL 
      NGFNETPTD HYMRPYYLA SLGERRKWP RLCTGEIPR 
      HQVMFNQMK NFFTVYKQK PKFLFGFHG ELSHDDYNL 
      VGVADDDFY KFLQFMKDS GVLDDTILI IMADHGHRF 
      AEIRNTIQG KQEERLPFF SFTFPESFK NHFKQAYEN 
      FQNNINKLS TPFDIHFTL KSLLELNDI GEANINYRA 
      VSLFTKIPA ERSCSHAYI EPHWCACLD WEKIPLSDP 
      IVKRISNLL ISTLNDYTK PHRHICEVL SVSDIEWVM 
      KMTPNENLI KYNKNADLD GFVADLSAK MRIKTNMYQ 
      IKVVVTPGD SVFEASVTY YIDEDKLNL KMSDISRVN 
      MYGKQARCV EDNLPDLRK FCYCKD
    CDS sequence
    • >TC014008 RA
      atgcaaatc agaaaagtg tttcttctg gccatttct 
      tcagccttt ggtattctg atgttactt atagtaaca 
      cgggactct ccaataaat gacatcgac cgaaaattc 
      cgattggaa aaaatcgac gaactgatt cactcacag 
      gcgagctcc ctggcttgc aaacagccc aacttgccg 
      gtgttttcc cccgaaatg atgaaattc gtccatgag 
      gagcccccg attgactgc tcatcggca gggccggac 
      tgggtcaaa tgccaaggt agcgagtgt cgaatccag 
      gacgaaatt aaggcgaga cacggacgc gtcacttgt 
      tctttcact gatataatc cgagtcaat gattttgag 
      tcaaccagc ggggaaaca acctactca gactcctac 
      tataaactc gaagcgagc gatgttgtc aaagtttcg 
      tgtcggggg caaaaggcc caatggtcg gcgactttg 
      accggaatt cgcctcgat aaaaaaatt tgggagaac 
      accagttgg agtaaacta ccctcggac gctgtgaag 
      ttcaacgtt ttgatgttc ggtttcgac tcaatttcg 
      cgcaactct ttcgtgcga aagttgccc aaaagctac 
      aagtttctg actgaaata ttgcacggg gacgttcta 
      caaggttat aatataatc ggggacgga accccgcaa 
      gccctaata ccgatttta acggggaag accgaactg 
      gaactccct gatacgaga aaacggttc gcaaacacg 
      aaattcgtg aactcgtac ccgtttatt tggaatgag 
      tacaagaag gcggggtac gtgactggt tactttgag 
      gatgtgccc gaactgggg atttttaca tatcgccta 
      aatggtttc aatgaaact ccaactgat cactacatg 
      cggccgtat tacctcgct tcgttgggg gaaagacgc 
      aaatggcca agactttgt accggggaa ataccgagg 
      catcaagtc atgttcaat cagatgaaa aattttttc 
      acagtttat aaacaaaaa cccaagttt ttgtttggt 
      tttcatgga gagttgtca catgatgat tataatttg 
      gtgggggtt gctgacgac gatttttac aagttctta 
      cagtttatg aaggactcg ggggttttg gatgatacc 
      attttgatt attatggca gatcatggg cacagattt 
      gcagaaatc agaaacacg attcaaggt aaacaagag 
      gaacgttta ccatttttc tcatttacg tttcccgaa 
      agtttcaaa aatcacttc aaacaagct tacgaaaat 
      tttcaaaat aacattaat aagttatca accccgttt 
      gatatccat tttacgttg aaaagtttg ctggaattg 
      aacgatatt ggggaggca aatatcaat tatagagca 
      gtgtcactg tttacaaag attccggct gagaggagc 
      tgctcccac gcgtacatt gagccccac tggtgtgcg 
      tgtctcgac tgggaaaaa atccccctt tctgacccg 
      atagtaaaa cgaatatca aatttacta atttccact 
      ttaaacgat tacacgaaa ccgcaccgc cacatatgt 
      gaggtgtta tcagtgtct gatattgaa tgggtgatg 
      aaaatgaca cccaatgaa aacctcata aagtacaat 
      aaaaacgcc gacttggat ggttttgtt gccgatttg 
      tcggcgaaa atgcgaatc aaaacgaat atgtatcaa 
      attaaagtg gttgtgacg cctggagat tcagttttt 
      gaagccagt gtgacgtat tacattgat gaagataag 
      ttgaatctt aaaatgagt gatatcagt cgagtcaac 
      atgtatgga aaacaagcg aggtgtgtg gaggataac 
      ctacctgat ttgagaaag ttttgttat tgtaaagat 
      tga
    mRNA sequence
    • >TC014008 RA
      tgacaaatc ggtgtgaca aatagcccc accggcgtt 
      tgtcatcat gttttgttg tgtttcgta acaaattca 
      atggatttg ttccataaa gcgttgaaa acaatactt 
      tcgttatca tgattgtga taaggcacc ccaaatgct 
      attaaaggc gtgttgcta gtggacggt tttagttac 
      aaaatgcgc ataattaga acaatgcaa atcagaaaa 
      gtgtttctt ctggccatt tcttcagcc tttggtatt 
      ctgatgtta cttatagta acacgggac tctccaata 
      aatgacatc gaccgaaaa ttccgattg gaaaaaatc 
      gacgaactg attcactca caggcgagc tccctggct 
      tgcaaacag cccaacttg ccggtgttt tcccccgaa 
      atgatgaaa ttcgtccat gaggagccc ccgattgac 
      tgctcatcg gcagggccg gactgggtc aaatgccaa 
      ggtagcgag tgtcgaatc caggacgaa attaaggcg 
      agacacgga cgcgtcact tgttctttc actgatata 
      atccgagtc aatgatttt gagtcaacc agcggggaa 
      acaacctac tcagactcc tactataaa ctcgaagcg 
      agcgatgtt gtcaaagtt tcgtgtcgg gggcaaaag 
      gcccaatgg tcggcgact ttgaccgga attcgcctc 
      gataaaaaa atttgggag aacaccagt tggagtaaa 
      ctaccctcg gacgctgtg aagttcaac gttttgatg 
      ttcggtttc gactcaatt tcgcgcaac tctttcgtg 
      cgaaagttg cccaaaagc tacaagttt ctgactgaa 
      atattgcac ggggacgtt ctacaaggt tataatata 
      atcggggac ggaaccccg caagcccta ataccgatt 
      ttaacgggg aagaccgaa ctggaactc cctgatacg 
      agaaaacgg ttcgcaaac acgaaattc gtgaactcg 
      tacccgttt atttggaat gagtacaag aaggcgggg 
      tacgtgact ggttacttt gaggatgtg cccgaactg 
      gggattttt acatatcgc ctaaatggt ttcaatgaa 
      actccaact gatcactac atgcggccg tattacctc 
      gcttcgttg ggggaaaga cgcaaatgg ccaagactt 
      tgtaccggg gaaataccg aggcatcaa gtcatgttc 
      aatcagatg aaaaatttt ttcacagtt tataaacaa 
      aaacccaag tttttgttt ggttttcat ggagagttg 
      tcacatgat gattataat ttggtgggg gttgctgac 
      gacgatttt tacaagttc ttacagttt atgaaggac 
      tcgggggtt ttggatgat accattttg attattatg 
      gcagatcat gggcacaga tttgcagaa atcagaaac 
      acgattcaa ggtaaacaa gaggaacgt ttaccattt 
      ttctcattt acgtttccc gaaagtttc aaaaatcac 
      ttcaaacaa gcttacgaa aattttcaa aataacatt 
      aataagtta tcaaccccg tttgatatc cattttacg 
      ttgaaaagt ttgctggaa ttgaacgat attggggag 
      gcaaatatc aattataga gcagtgtca ctgtttaca 
      aagattccg gctgagagg agctgctcc cacgcgtac 
      attgagccc cactggtgt gcgtgtctc gactgggaa 
      aaaatcccc ctttctgac ccgatagta aaacgaata 
      tcaaattta ctaatttcc actttaaac gattacacg 
      aaaccgcac cgccacata tgtgaggtg ttatcagtg 
      tctgatatt gaatgggtg atgaaaatg acacccaat 
      gaaaacctc ataaagtac aataaaaac gccgacttg 
      gatggtttt gttgccgat ttgtcggcg aaaatgcga 
      atcaaaacg aatatgtat caaattaaa gtggttgtg 
      acgcctgga gattcagtt tttgaagcc agtgtgacg 
      tattacatt gatgaagat aagttgaat cttaaaatg 
      agtgatatc agtcgagtc aacatgtat ggaaaacaa 
      gcgaggtgt gtggaggat aacctacct gatttgaga 
      aagttttgt tattgtaaa gattgaaaa atattgctc 
      agtgtttga gtgactaaa actacaaat gtgagtgta 
      gggggcagc accagaaca aatcgtttt ctttatcag 
      tatttcaag ttgcagcac tttataaaa gggacaact 
      tgaattgtt ttacatgat caattacat aattgtttt 
      tgtttttat taactgttt attttgtac ataatataa 
      tgattttca aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY