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Gene information
General information
TC013971 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3299955..3304882
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013971
    iBeetle screen iB_02232

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013971 RA
      MGGSSKSSS TDSSNPRHQ VQFGITRDE SEESVELDL 
      PSEHSETEI ENVNERLRH QTRLPDDDY QIKNLSRVL 
      AVVTTVMLQ MCYQKIAFF RTMNKMPRV PINLEYVPL 
      DEKYNNAER EIGVILHPS EEFLNYRKF QKDLSWLEN 
      VLKSVIVDL QKSFYFDDF IKILNEDEM TRQEEQTLL 
      TSKMFNLHE VKKLRTYLD LEKKELTRE LQDVTEELG 
      NFKDQLNDD IIESVVKLN YVKEWQKSR GEQNTSILN 
      RQEQKLIKM SENMTTEIE KEIVIHDEM CTFLEMSLD 
      EFQEEITKW MEKYDMDTE ELEKAVEKK KIELEQQKD 
      NHSKLLEEF NYRQEEMDK YKAHKLQMQ KEAEEIQRQ 
      TRAATRIQA WWRGVMFRK GLGPFKRKK GKGKKGSGS 
      AKKHKK
    CDS sequence
    • >TC013971 RA
      atggggggc tccagtaaa agctccagc accgacagt 
      agcaacccc cgccaccaa gtccaattc ggcatcact 
      cgcgacgaa agcgaggaa agcgtcgaa ttggactta 
      ccatccgaa cattcggaa accgaaatt gaaaacgtc 
      aacgagcgc ctccgccac caaacgcgt ctacctgac 
      gatgactac caaattaaa aatttgagc cgagttttg 
      gccgtcgtg accacagtc atgctccaa atgtgttac 
      caaaaaatc gcttttttc cgaacgatg aataaaatg 
      cccagagta cccataaat ctcgaatat gtgccactg 
      gacgagaaa tacaacaat gcggagcgc gagattggg 
      gttatttta cacccgagt gaggagttt ctcaattac 
      agaaagttt caaaaggat ttgtcgtgg cttgagaat 
      gtcttaaaa tcggttatt gttgatttg caaaaaagt 
      ttctatttc gatgatttt atcaaaatt ttgaacgag 
      gacgagatg acccggcag gaggagcaa acattgctc 
      acctcgaag atgtttaat cttcatgaa gtgaagaag 
      ttgaggacg tatctcgat ttagagaag aaagaactt 
      accagagaa ttgcaagat gttactgaa gagttgggc 
      aattttaaa gaccaatta aacgacgac attattgaa 
      agcgtagtt aaactaaat tacgtaaaa gagtggcaa 
      aaatcgcga ggagagcaa aacacaagt attctaaat 
      cgccaagaa cagaaactt atcaaaatg tccgaaaat 
      atgactaca gaaattgag aaagaaatt gtcattcat 
      gacgaaatg tgcactttc ctggaaatg tctctcgat 
      gaatttcag gaagaaatc accaagtgg atggaaaag 
      tatgacatg gatacggag gaactggaa aaggcggtc 
      gaaaagaag aaaatcgaa ttggaacag cagaaggac 
      aatcactca aaattacta gaagagttc aattatcgc 
      caagaagag atggacaaa tacaaagcc cacaaactc 
      caaatgcaa aaagaggcc gaggagatc caacggcag 
      acccgagca gcgacccgg atccaggcc tggtggcgc 
      ggcgtaatg ttccgcaaa ggcctaggc cccttcaaa 
      cgcaaaaaa gggaaaggg aagaaagga agtggatcg 
      gcaaaaaaa cacaaaaaa taa
    mRNA sequence
    • >TC013971 RA
      agtgacaaa cacgtgcgc ttcccttct acccctttc 
      actcaaaaa tcgcgtccc aaccagccc gggctgttg 
      aaactccct cgatgtaaa ccgccccaa acgcaaaat 
      ggggggctc cagtaaaag ctccagcac cgacagtag 
      caacccccg ccaccaagt ccaattcgg catcactcg 
      cgacgaaag cgaggaaag cgtcgaatt ggacttacc 
      atccgaaca ttcggaaac cgaaattga aaacgtcaa 
      cgagcgcct ccgccacca aacgcgtct acctgacga 
      tgactacca aattaaaaa tttgagccg agttttggc 
      cgtcgtgac cacagtcat gctccaaat gtgttacca 
      aaaaatcgc ttttttccg aacgatgaa taaaatgcc 
      cagagtacc cataaatct cgaatatgt gccactgga 
      cgagaaata caacaatgc ggagcgcga gattggggt 
      tattttaca cccgagtga ggagtttct caattacag 
      aaagtttca aaaggattt gtcgtggct tgagaatgt 
      cttaaaatc ggttattgt tgatttgca aaaaagttt 
      ctatttcga tgattttat caaaatttt gaacgagga 
      cgagatgac ccggcagga ggagcaaac attgctcac 
      ctcgaagat gtttaatct tcatgaagt gaagaagtt 
      gaggacgta tctcgattt agagaagaa agaacttac 
      cagagaatt gcaagatgt tactgaaga gttgggcaa 
      ttttaaaga ccaattaaa cgacgacat tattgaaag 
      cgtagttaa actaaatta cgtaaaaga gtggcaaaa 
      atcgcgagg agagcaaaa cacaagtat tctaaatcg 
      ccaagaaca gaaacttat caaaatgtc cgaaaatat 
      gactacaga aattgagaa agaaattgt cattcatga 
      cgaaatgtg cactttcct ggaaatgtc tctcgatga 
      atttcagga agaaatcac caagtggat ggaaaagta 
      tgacatgga tacggagga actggaaaa ggcggtcga 
      aaagaagaa aatcgaatt ggaacagca gaaggacaa 
      tcactcaaa attactaga agagttcaa ttatcgcca 
      agaagagat ggacaaata caaagccca caaactcca 
      aatgcaaaa agaggccga ggagatcca acggcagac 
      ccgagcagc gacccggat ccaggcctg gtggcgcgg 
      cgtaatgtt ccgcaaagg cctaggccc cttcaaacg 
      caaaaaagg gaaagggaa gaaaggaag tggatcggc 
      aaaaaaaca caaaaaata aacttctaa aactataat 
      tcccaaatt aatttacaa ttctttata acctcttcg 
      ggctccgtg tgtttataa aacatgaaa aatccctga 
      atctgggcc ggactgaca ttttttccg aacgcggcc 
      actttctca gcgaactcc ttcgcctgc cttcgggca 
      ttttcgcca tcgtaaaac cgtaggaac atctgctcg 
      atctgatag ggggaacac cagccgatg tattccttc 
      aaatcaact cggccgggc cgtatcagg gccggatcc 
      agccgttcg aggtaattc gttgtcata aaaacaatc 
      cgggcttcg gtgctggcc accccgtcc aagcagttc 
      aaaagaccg ctgaaagtg acccgattt agcccctca 
      tatgcggac ttttgctgg ggggtgtcc tcccgtgaa 
      acgaaagca gcatcgatg tcctccaga aggatgatg 
      ctctgctgg ggggccaca ctcaagaga tgattcagc 
      ctgtcatcc gacagcccc ctctccgat aaattcaaa 
      acacaaatc gaaaagccc aactcgccg gccagggcc 
      gtaatgtac gaagatttg ccgcaacca ggcggcccg 
      tgcaacaaa taacctaca aatgagcta agcaatgaa 
      gaggccgag ttggggtca cgtgacgta cctctccga 
      taggggatt ccccgttcc gtgtaccac gaagggttt 
      gaaatgaat tctttacag tcatttaaa attttgtca 
      ccgatgttt tcatcgagg attacagag gcgatgggg 
      cgtcggcgg cgtgggtgc cccaaaggg cgccattcg 
      ctacccatt gctgtgtac atgatggtt ttgccctcg 
      tgctgccgc aaagccatc tgtctggct aacaaattt 
      aagttgaca tttcacaaa atcacacca cttttgctt 
      tttgagtgc tttttacag ccacaaacg caattatta 
      cgcaactaa attgtgtta tttttgttt agaaaaatt 
      ttgccacca aattagata gaatagacg ttattttag 
      tttgttgcg ctcgattta gttcatttg cttcactcg 
      acttcagca agaattcgt caaaacaaa aactgaaaa 
      aattattcc taaataatt tcgtcaaaa cacaaaaat 
      ctaaattac caagtaaag tctaaaata acatttcga 
      gccaaaatt ttccaattt tcaagtgcc ccaaatgta 
      gaagcacga tagcgcttt aattggtag ttaaaaggt 
      gcacttcga cccaaaata aaattaaat ggaacga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY