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Gene information
General information
TC013946 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2752930..2759664
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013946
    iBeetle screen iB_02230

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013946 RA
      MEIEEIVDF AKHKTPEVK LKKQNSQDL QMTSSTTSS 
      LASPGDSGV QLLDSESEI TSVMSVSGL GCDIDTIPN 
      LDNDEKTAD NKEFNQNNF NHFATSVPN YFDKFEAKC 
      LEKSKKEVL NKSLSEEVF EMEHPIKDS DLMNVSLNS 
      YELLDYTLQ NKLATNIDQ MHVSMSEEV NENLKVNGQ 
      NPEPVVLPI AEDKPEEQI VFRRQRKKK SKSDTPKKR 
      VSFHEDILN STKIDDIHI NHGFITHEP DVSLSFFQR 
      GFVRKPDVV KGRYSWAAE GDAPYYEKT PSDREIKSD 
      LYCQHNTRF SSTSSSSTG SISSSIDED DETCVKKEP 
      KFMQPKSSC LKKTKHSKK IDTKIVQEE TNRKRRSDS 
      NLLDSNIFG SLKNILTFS TSVPLAERG VPEGQEDFA 
      IYSSSHESN RRKSCTNLT FKGFEPIEA QPVPAKTEK 
      PLSANKTNL KLTASEGFY PNYPTNQNL PANVIVCDS 
      NVYEHKGIS YSYEYDKFQ KSFEQQNKP KSSTVYQMI 
      LKEFNFFRK KAKEPEKEA PDDFEIVPS PVPEKSDSI 
      EEVQEVEYK SPKNSISRY ASTTKMDWS DNETISDMS 
      ENPTGRHLH SPKRRVSKS NHYSLSPFK VAPSESGKS 
      DQEGALVSK SLQNLKPAT SKSSLINRF LRNVTLKKM 
      LDAKAQNKQ KNCRKYMGL YVKGVKFEY KGRDEVDEA 
      LEREILKGM EVAQSSEDV VDKKFGMQL KKEIFRSRM 
      ERLIRIFPV RSAYATNGE SKPLLLILS DTTLYITGI 
      KLGNSLCNH FVLPYTELN TILIGPSAQ TIHLSNYDK 
      DMQCIITTG CGKITGDIV GQLEVAMRR DMSKPRLPA 
      VKHLSMRDM VNLRRAICK QTSVDKEEE YFYYSIVNI 
      QDFNPDIPE VTPLGPNKE GPLMFKTSD TEARWETAY 
      FILKAGVLY MLSSASQRV PMRVFPLIN GSCQGAHRV 
      FNCPRPHTF QLIVEGKSL HLAAPDEYV ASDWLQCLV 
      HAASGGYNQ KEKMLTQSC SLLMTSDHI LTVREAFPC 
      TVSSLLPSK NQHQPIKGP QVLSCAAII DLVAFRLPS 
      AEQSWCVLE FSCREVHEC SGDWILYFA TNVELENFI 
      STLEVLWTY NNENGDSFP LSTIPESDP LSKKCVDIY 
      TSLKQLWPS NSVHLQFL
    CDS sequence
    • >TC013946 RA
      atggaaatt gaagaaatc gtcgatttt gcaaagcac 
      aaaacaccg gaagtgaaa ctcaagaaa cagaacagc 
      caagatctg caaatgaca tcaagtaca acctcgagt 
      ttggcctcg ccaggggac tcaggagtg caattgttg 
      gacagcgag agcgaaatc acgtctgtg atgtccgtt 
      agcggactc ggttgtgat attgatacg ataccaaac 
      ctggataac gacgaaaaa accgctgac aataaagaa 
      ttcaatcaa aacaatttc aaccatttc gcaacgtcc 
      gtacccaac tatttcgac aagttcgag gcaaaatgc 
      ctggaaaag tcgaagaag gaggtgctg aacaagtcg 
      ctaagcgag gaagtgttc gaaatggaa cacccgatc 
      aaagactcg gacttgatg aacgtctcc ttgaacagc 
      tacgaactg ctagactac accctccag aacaaactt 
      gcgacaaac attgaccaa atgcacgtc agcatgtcc 
      gaagaagtg aacgaaaat ttgaaagtc aacggccag 
      aaccccgaa cctgtggtg ttgccgatc gctgaagac 
      aaaccggaa gagcagatc gtcttccgg cgccaacgc 
      aagaaaaaa tccaagtcg gacacccca aaaaagcgc 
      gtctcgttc cacgaggat attttaaac agcacgaaa 
      atcgacgat attcacatt aatcacggc tttatcacg 
      cacgagccc gacgtctcg ctcagcttt ttccaaagg 
      ggcttcgtc cggaagccg gacgtggtc aaggggcgc 
      tacagctgg gccgccgaa ggcgacgcc ccttactac 
      gagaaaacc ccctcagac agagaaatc aaatcggac 
      ctgtattgc caacacaac acccgcttc tcgtcaact 
      tcgtcaagc tccaccgga agcatctcc tcgtcaatc 
      gacgaagac gacgagacc tgtgtgaag aaagagccc 
      aagttcatg caaccgaag tcgagttgt ctgaagaaa 
      accaaacac tcgaagaaa atcgacacg aagatcgtg 
      caggaggaa acgaaccgg aaacgaaga tctgacagt 
      aatttactc gattcgaat attttcggc agtttgaag 
      aacatttta acgttttcg acttcggtt cctttggcg 
      gagcgtgga gtcccagaa ggccaggaa gactttgcg 
      atttattcg tcttcacat gagtccaac cggcgtaaa 
      tcctgcacg aatttaact tttaaaggg tttgaaccg 
      atcgaggca caaccggtt ccggctaaa accgaaaaa 
      cctctctct gccaacaaa acaaacctg aagttgacg 
      gcaagcgag ggcttttac ccgaattac cccaccaat 
      caaaaccta ccagcgaat gttatcgtg tgtgatagt 
      aacgtgtac gagcataaa gggatcagt tattcgtac 
      gaatatgac aagttccag aagtcgttc gagcagcaa 
      aacaaaccg aaaagctca accgtctac caaatgatc 
      ctgaaagag tttaatttt tttcgcaag aaggctaag 
      gaaccggaa aaggaagct ccggacgat ttcgagatc 
      gtgccgagt ccggtgccg gaaaaaagc gacagtatt 
      gaagaagtc caagaggtg gagtacaaa agtccgaaa 
      aactccatc agtagatat gcttcaacc accaaaatg 
      gattggtcc gataatgaa accatctca gacatgtcc 
      gaaaatccc actgggcgc catttgcac tcgccgaag 
      aggcgggtg agtaaaagt aatcattac tcgttgagt 
      ccgtttaaa gttgcgcca agtgagtcg gggaaaagt 
      gaccaagag ggggcgcta gttagtaaa agtttgcaa 
      aatttaaaa ccagccact tcgaagtcc tcgctgatc 
      aatcggttt ttgcgcaac gttacgctg aagaaaatg 
      ttggacgcc aaagcgcaa aataagcaa aagaattgt 
      cgcaagtat atggggttg tatgtgaaa ggggtgaag 
      tttgagtat aaggggagg gatgaggtt gatgaggcg 
      ttggaacgg gaaattctg aaagggatg gaagtggct 
      cagagttcc gaagatgtg gtcgataag aagtttggg 
      atgcagttg aagaaggag atctttagg agcagaatg 
      gaacggctt ataaggatt ttccccgta cgaagcgct 
      tacgcaaca aacggcgaa agcaaacct ttgctccta 
      atcctaagc gacacaact ctctacata acaggaata 
      aaattggga aattcgctt tgcaatcat tttgttctg 
      ccctacacc gagctcaat acaattcta atagggcca 
      agtgcacaa accatacac ttatccaat tacgataaa 
      gacatgcaa tgtattata acaacgggt tgtgggaaa 
      attacgggt gatattgtg ggccagctt gaagtggca 
      atgagacgt gatatgagt aaaccacga ctgccagcc 
      gtcaaacat ctaagtatg cgagacatg gtgaatctg 
      cggagagcg atttgcaaa caaacatca gtcgacaag 
      gaggaagaa tatttctat tatagcatc gtaaacatt 
      caagatttt aacccggat ataccggaa gtgacgcct 
      ctaggcccc aacaaggag ggccctctg atgttcaaa 
      acctcagac actgaggca cgatgggaa accgcttac 
      ttcatccta aaggctggt gttctttat atgctgtca 
      tcggcttcg caaagggtc ccaatgcgt gtgtttccc 
      ctaataaat ggttcctgt cagggggct catcgtgtc 
      ttcaactgc cctcggccc cacacattc cagttgatc 
      gtcgaaggg aaaagtctg catctggca gcccccgac 
      gaatacgta gccagtgac tggttgcag tgcctcgta 
      cacgcagcc agcgggggc tacaaccag aaagagaaa 
      atgttgact caatcctgc tctcttctg atgacgagt 
      gaccatatt ttgacagtg cgggaggct ttcccctgc 
      acagtctcc tctctcttg ccctcgaaa aaccagcat 
      cagcccatt aaaggacct caagtatta tcatgtgct 
      gcgattatt gatttggtg gcgtttcga ttgccttcg 
      gccgagcaa agttggtgt gttttggag ttctcctgt 
      agagaggta cacgagtgt agcggcgat tggattttg 
      tattttgcg acgaacgtc gagcttgag aattttata 
      tcaactttg gaggttctc tggacttac aataatgaa 
      aacggtgat agttttccg ttgagtact atcccggag 
      agcgaccct ttgagtaag aaatgcgtg gatatttat 
      acttctttg aaacaacta tggccatct aatagtgta 
      catttacaa tttctgtaa
    mRNA sequence
    • >TC013946 RA
      gtcaaaaag taacagcaa acgcatgct caaaagtgt 
      gatcagtgg agcaccctt aaaaataat aatgacgat 
      ttactttat tacgtgcaa tgtagttaa aaaaatgat 
      actctttta atatcccac cacaagaaa aaccacaac 
      gaggacaat aatagcaag caacaatgg aaattgaag 
      aaatcgtcg attttgcaa agcacaaaa caccggaag 
      tgaaactca agaaacaga acagccaag atctgcaaa 
      tgacatcaa gtacaacct cgagtttgg cctcgccag 
      gggactcag gagtgcaat tgttggaca gcgagagcg 
      aaatcacgt ctgtgatgt ccgttagcg gactcggtt 
      gtgatattg atacgatac caaacctgg ataacgacg 
      aaaaaaccg ctgacaata aagaattca atcaaaaca 
      atttcaacc atttcgcaa cgtccgtac ccaactatt 
      tcgacaagt tcgaggcaa aatgcctgg aaaagtcga 
      agaaggagg tgctgaaca agtcgctaa gcgaggaag 
      tgttcgaaa tggaacacc cgatcaaag actcggact 
      tgatgaacg tctccttga acagctacg aactgctag 
      actacaccc tccagaaca aacttgcga caaacattg 
      accaaatgc acgtcagca tgtccgaag aagtgaacg 
      aaaatttga aagtcaacg gccagaacc ccgaacctg 
      tggtgttgc cgatcgctg aagacaaac cggaagagc 
      agatcgtct tccggcgcc aacgcaaga aaaaatcca 
      agtcggaca ccccaaaaa agcgcgtct cgttccacg 
      aggatattt taaacagca cgaaaatcg acgatattc 
      acattaatc acggcttta tcacgcacg agcccgacg 
      tctcgctca gctttttcc aaaggggct tcgtccgga 
      agccggacg tggtcaagg ggcgctaca gctgggccg 
      ccgaaggcg acgcccctt actacgaga aaaccccct 
      cagacagag aaatcaaat cggacctgt attgccaac 
      acaacaccc gcttctcgt caacttcgt caagctcca 
      ccggaagca tctcctcgt caatcgacg aagacgacg 
      agacctgtg tgaagaaag agcccaagt tcatgcaac 
      cgaagtcga gttgtctga agaaaacca aacactcga 
      agaaaatcg acacgaaga tcgtgcagg aggaaacga 
      accggaaac gaagatctg acagtaatt tactcgatt 
      cgaatattt tcggcagtt tgaagaaca ttttaacgt 
      tttcgactt cggttcctt tggcggagc gtggagtcc 
      cagaaggcc aggaagact ttgcgattt attcgtctt 
      cacatgagt ccaaccggc gtaaatcct gcacgaatt 
      taactttta aagggtttg aaccgatcg aggcacaac 
      cggttccgg ctaaaaccg aaaaacctc tctctgcca 
      acaaaacaa acctgaagt tgacggcaa gcgagggct 
      tttacccga attacccca ccaatcaaa acctaccag 
      cgaatgtta tcgtgtgtg atagtaacg tgtacgagc 
      ataaaggga tcagttatt cgtacgaat atgacaagt 
      tccagaagt cgttcgagc agcaaaaca aaccgaaaa 
      gctcaaccg tctaccaaa tgatcctga aagagttta 
      atttttttc gcaagaagg ctaaggaac cggaaaagg 
      aagctccgg acgatttcg agatcgtgc cgagtccgg 
      tgccggaaa aaagcgaca gtattgaag aagtccaag 
      aggtggagt acaaaagtc cgaaaaact ccatcagta 
      gatatgctt caaccacca aaatggatt ggtccgata 
      atgaaacca tctcagaca tgtccgaaa atcccactg 
      ggcgccatt tgcactcgc cgaagaggc gggtgagta 
      aaagtaatc attactcgt tgagtccgt ttaaagttg 
      cgccaagtg agtcgggga aaagtgacc aagaggggg 
      cgctagtta gtaaaagtt tgcaaaatt taaaaccag 
      ccacttcga agtcctcgc tgatcaatc ggtttttgc 
      gcaacgtta cgctgaaga aaatgttgg acgccaaag 
      cgcaaaata agcaaaaga attgtcgca agtatatgg 
      ggttgtatg tgaaagggg tgaagtttg agtataagg 
      ggagggatg aggttgatg aggcgttgg aacgggaaa 
      ttctgaaag ggatggaag tggctcaga gttccgaag 
      atgtggtcg ataagaagt ttgggatgc agttgaaga 
      aggagatct ttaggagca gaatggaac ggcttataa 
      ggattttcc ccgtacgaa gcgcttacg caacaaacg 
      gcgaaagca aacctttgc tcctaatcc taagcgaca 
      caactctct acataacag gaataaaat tgggaaatt 
      cgctttgca atcattttg ttctgccct acaccgagc 
      tcaatacaa ttctaatag ggccaagtg cacaaacca 
      tacacttat ccaattacg ataaagaca tgcaatgta 
      ttataacaa cgggttgtg ggaaaatta cgggtgata 
      ttgtgggcc agcttgaag tggcaatga gacgtgata 
      tgagtaaac cacgactgc cagccgtca aacatctaa 
      gtatgcgag acatggtga atctgcgga gagcgattt 
      gcaaacaaa catcagtcg acaaggagg aagaatatt 
      tctattata gcatcgtaa acattcaag attttaacc 
      cggatatac cggaagtga cgcctctag gccccaaca 
      aggagggcc ctctgatgt tcaaaacct cagacactg 
      aggcacgat gggaaaccg cttacttca tcctaaagg 
      ctggtgttc tttatatgc tgtcatcgg cttcgcaaa 
      gggtcccaa tgcgtgtgt ttcccctaa taaatggtt 
      cctgtcagg gggctcatc gtgtcttca actgccctc 
      ggccccaca cattccagt tgatcgtcg aagggaaaa 
      gtctgcatc tggcagccc ccgacgaat acgtagcca 
      gtgactggt tgcagtgcc tcgtacacg cagccagcg 
      ggggctaca accagaaag agaaaatgt tgactcaat 
      cctgctctc ttctgatga cgagtgacc atattttga 
      cagtgcggg aggctttcc cctgcacag tctcctctc 
      tcttgccct cgaaaaacc agcatcagc ccattaaag 
      gacctcaag tattatcat gtgctgcga ttattgatt 
      tggtggcgt ttcgattgc cttcggccg agcaaagtt 
      ggtgtgttt tggagttct cctgtagag aggtacacg 
      agtgtagcg gcgattgga ttttgtatt ttgcgacga 
      acgtcgagc ttgagaatt ttatatcaa ctttggagg 
      ttctctgga cttacaata atgaaaacg gtgatagtt 
      ttccgttga gtactatcc cggagagcg accctttga 
      gtaagaaat gcgtggata tttatactt ctttgaaac 
      aactatggc catctaata gtgtacatt tacaatttc 
      tgtaattta acgcttgat ttcctgttg gttttctgt 
      atatatggc cgctttagt tgtagggtg atatattta 
      tttttctag ccatacaat tccaaattt attttgata 
      ctctagtca ttttttctt ttatttatt gatatacaa 
      atgtgatag atatttaaa tagtttttg tatcaacgc 
      ttgctgtaa tgcacatgt agacaaagc acagctcct 
      tttgttaaa ttattacgt agcatggta gtcataatt 
      gtcgctctt tatggcttg ggtttggcc gattttaac 
      tgattttat ttcaagttt tagatgaac tgtgatatt 
      ttgttaccg ttttaattt agaattgtc caaatcttt 
      caagccgta aaggtaaat tatttaaag ggattgata 
      ttgaacata ttaggaaat accgtttct ctcaatttt 
      gaaaatatt caaaggcca gtttaatcg tttaaatcg 
      ttaccccgt gtagcttgg atggtgctt gacccagtt 
      tatatcaaa tgtaatttt agttaggga aataatttg 
      gtgctgttg aaaattgtt taaatttgt taagggttt 
      caaacgttg ggtgatgta gtgatattt gatttaatt 
      ccaacttac acaagtatt ttttgctca gattaccta 
      aatactgac ccattatta ttaacttca ctgtactaa 
      ataatgtag tcctcaata ctcaataaa ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY