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Gene information
General information
TC013927 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2541369..2547392
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013927
    iBeetle screen iB_02229

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013927 RA
      MSKDIRSFF TVISKKSDA NSPKAASPA VKKKPKRII 
DSDDEDVIS ATPDKEQKK VEKKREVLS SDSDEDAKK 
KRKTEEPKL KPVNVADVF KNSPIKQSK VEVPQTPEV 
KPTKAEEKK PKNRKKKHD AEKEIHNNS AFNKTLEDL 
DDDIFEQSI HLIESIEEA YQGDKEKKE SKNESDSQV 
QSAKKRSRN DSSSDNQTP KKKKIEHSD SGIDPGQEA 
HEKKRYSAM LYQRYLNRG EPKHLGMKE LPKGKPNCL 
QNLCFLRTG VLDSLDSEE FANIIKQHG GRVVHAVSK 
KVNYVVVGA EPGPAKLAK AESYNIPNI SEDELLDMI 
LTKSGMEPK YCKKNTSCD SEDLGIDLN HETKPSKEK 
ISNKTEPKK ETPEKKSSV KKQNGTAPS KIEQEIKSE 
TLSSNSFYS QPGTSKVSE STKIENKVE AVQLSLPEK 
YKPQTLRAV IGQQGDSSN LAKLKHWLE NWYKNQDPK 
VKKTLARPS PWSTKKDDG AYFKCALLS GPPGVGKTT 
TATLVAKEL GLDIVEFNA SDTRSKKLL HEEVAQLLS 
TKTIAGYAT GQPNVNRNR VLLMDEVDG MAGNEDRGG 
IQELIQLIK NSSVPIICM CNDRNHQKI RSLVNYCFD 
LKFTKPKLE QIRGAMMSI CCKENIDVS TQALTEIIA 
GTGCDVRQT LNHLALLGS TKGEITVEV AEKESKASK 
KDTVMGPWE VCRTVFTKS EHKDMSVAD RARLFFFDY 
SLGPLFIQE NYLRVQPEG SKKEGLLKA ALTADSISV 
GDIIESKIR RTNNWALLE SQAFFSSVI PGHYMSGHF 
NSQINFPAW LGKNSKKNK FKRLISELQ SHTRTSASA 
SKMAVKLDY LVPLRNAIF TPLIKNGLE GINQAVGVM 
KDYNLLRED VDSLIELCQ WLQGENPMN SIDSKVKSA 
FTRTYNKQV TLLPYAPAS GVSKKKAAA LESDLLDEL 
DEISDNEDD DDISKNAMI KVKKKTAPK GKKADEGKG 
TSKNEKKGK AKKK
    CDS sequence
    • >TC013927 RA
      atgtcgaaa gacatccgt tcctttttc actgtaatt 
tcaaagaag tctgatgca aattcgccc aaagctgct 
tctcctgcc gttaaaaag aagccaaaa cgtatcata 
gactccgat gatgaggac gtgatttct gccactcca 
gacaaagag cagaagaag gttgagaag aaacgtgaa 
gttctaagt tcagactct gatgaagac gcgaagaaa 
aaaaggaag actgaagaa ccgaaatta aaaccggtg 
aatgtggcc gacgtgttt aagaacagt cctataaaa 
cagagcaag gttgaagtg ccacaaaca cccgaagtc 
aagcctaca aaagcagaa gagaaaaaa cccaaaaat 
aggaagaaa aagcacgat gcggagaag gagattcac 
aacaatagt gctttcaac aagacgctg gaagactta 
gatgatgat attttcgaa cagagtatt catcttatt 
gagtcaatt gaggaagcg taccagggt gataaagaa 
aagaaagaa agtaaaaat gaatcagat agtcaagtt 
caaagcgca aagaaacga tcaagaaac gattcgtca 
agtgataat caaacacct aagaagaag aaaattgag 
cattcggac tctggtatt gatccggga caagaagct 
catgaaaaa aagagatac tcagccatg ctctaccaa 
aggtacctc aacagaggg gaacccaaa catcttgga 
atgaaggaa ttgccaaaa gggaagccg aattgtttg 
caaaatttg tgtttccta agaactggc gttttagac 
tcgcttgat tcggaggag tttgcaaat ataataaaa 
caacacggt ggacgtgta gtgcacgca gtttcaaaa 
aaagtaaat tatgtggtt gtgggtgca gaaccaggg 
ccggcaaaa cttgcaaaa gctgaaagt tataacata 
cctaacata tctgaagat gagcttttg gacatgata 
cttacaaag tcagggatg gaaccgaaa tattgcaaa 
aagaatacg tcgtgtgat agcgaggat ttaggcatt 
gatttaaac catgaaaca aaacctagc aaagaaaaa 
ataagtaac aaaactgag cctaaaaaa gaaacgcct 
gaaaaaaaa tcgagtgtc aaaaaacaa aacggtacc 
gctccatcc aaaatagag caggaaatt aaaagtgaa 
actctgtca tctaacagt ttttatagc caacctggc 
actagtaaa gtttcggaa agtacaaaa atcgaaaat 
aaagtagag gcagttcag ttatccttg cctgaaaaa 
tacaagcca cagacactt cgagcagta ataggacaa 
caaggtgat tcaagtaat ttggccaaa ctcaaacac 
tggcttgaa aattggtac aaaaatcaa gacccgaaa 
gttaaaaaa acattagca cgtcctagt ccttggtct 
actaagaaa gatgatggt gcttatttc aagtgtgcg 
ttgttgagt ggtccacca ggtgttggt aaaaccaca 
actgcaaca cttgtggca aaggaattg ggtttggat 
atagtcgag tttaatgct tctgatacg aggagcaag 
aagttgcta catgaagaa gtggcgcag ttgttgagc 
actaaaaca atagctgga tatgcaaca ggccaaccg 
aatgtgaac cgcaaccgc gtgttgcta atggacgaa 
gtggacgga atggcagga aacgaggac cgcggcggc 
atccaggaa ctaatccag ctgattaaa aattcaagc 
gttccgata atttgcatg tgcaacgac cgaaatcac 
caaaaaatc cgatcactt gtcaactac tgcttcgat 
ttgaaattt accaaacca aaacttgaa caaattcgg 
ggggcaatg atgtcgatc tgttgcaaa gaaaacatc 
gatgtttcc acacaagca ttgactgaa attatcgcc 
ggaacgggc tgtgatgtc cgccagacg ctcaatcat 
ttggctctg ttgggatca accaaaggg gaaatcaca 
gttgaagtg gctgaaaag gagtcaaaa gcttcaaag 
aaagacact gtgatgggt ccatgggag gtgtgtcgg 
actgttttt accaaaagc gaacataag gatatgagt 
gtagctgac agagcgaga ttgtttttc ttcgattat 
agtttaggg ccgcttttt attcaagaa aattatttg 
agggtgcaa cccgagggt tcaaagaaa gaagggtta 
ctaaaggct gctttaacc gctgattct ataagtgtt 
ggcgatatt atagaatca aaaatcaga agaaccaat 
aattgggcg ttgcttgaa tcgcaagct ttcttttcg 
tctgttatt ccaggtcat tacatgtcc ggtcatttc 
aattcccaa attaatttc cccgcttgg ttggggaaa 
aattcgaag aaaaataag ttcaagagg ttgattagt 
gagctgcaa tcgcacaca cgtactagt gcatctgcg 
agcaaaatg gcagttaag ttggattat ttggtaccg 
ctccgaaat gcaattttt acaccactg attaagaac 
ggattggaa ggaatcaat caagcggtt ggtgtgatg 
aaggattat aacctcctg agagaagat gtcgatagt 
ttgatagaa ctgtgccaa tggttacaa ggagaaaat 
ccaatgaat agtatcgat tcgaaagta aaaagtgcg 
tttactcgc acatacaat aaacaagtc actctatta 
ccatatgct cctgcgtct ggagtttct aaaaagaaa 
gcggctgct ttggaaagt gatctcttg gacgaactt 
gatgaaatt tccgataat gaggatgat gacgatatt 
agtaaaaat gctatgatt aaggtgaaa aagaagaca 
gctcctaaa ggtaagaag gctgatgaa ggtaaaggt 
acaagtaaa aatgagaag aaaggaaaa gcgaagaaa 
aagtaa
    mRNA sequence
    • >TC013927 RA
      tgtgaatta aacaacaac ctgcaaaca gataataat 
ttgataaaa acaacagat aattttggg tgtaacgta 
atcgtacca tgtcgaaag acatccgtt cctttttca 
ctgtaattt caaagaagt ctgatgcaa attcgccca 
aagctgctt ctcctgccg ttaaaaaga agccaaaac 
gtatcatag actccgatg atgaggacg tgatttctg 
ccactccag acaaagagc agaagaagg ttgagaaga 
aacgtgaag ttctaagtt cagactctg atgaagacg 
cgaagaaaa aaaggaaga ctgaagaac cgaaattaa 
aaccggtga atgtggccg acgtgttta agaacagtc 
ctataaaac agagcaagg ttgaagtgc cacaaacac 
ccgaagtca agcctacaa aagcagaag agaaaaaac 
ccaaaaata ggaagaaaa agcacgatg cggagaagg 
agattcaca acaatagtg ctttcaaca agacgctgg 
aagacttag atgatgata ttttcgaac agagtattc 
atcttattg agtcaattg aggaagcgt accagggtg 
ataaagaaa agaaagaaa gtaaaaatg aatcagata 
gtcaagttc aaagcgcaa agaaacgat caagaaacg 
attcgtcaa gtgataatc aaacaccta agaagaaga 
aaattgagc attcggact ctggtattg atccgggac 
aagaagctc atgaaaaaa agagatact cagccatgc 
tctaccaaa ggtacctca acagagggg aacccaaac 
atcttggaa tgaaggaat tgccaaaag ggaagccga 
attgtttgc aaaatttgt gtttcctaa gaactggcg 
ttttagact cgcttgatt cggaggagt ttgcaaata 
taataaaac aacacggtg gacgtgtag tgcacgcag 
tttcaaaaa aagtaaatt atgtggttg tgggtgcag 
aaccagggc cggcaaaac ttgcaaaag ctgaaagtt 
ataacatac ctaacatat ctgaagatg agcttttgg 
acatgatac ttacaaagt cagggatgg aaccgaaat 
attgcaaaa agaatacgt cgtgtgata gcgaggatt 
taggcattg atttaaacc atgaaacaa aacctagca 
aagaaaaaa taagtaaca aaactgagc ctaaaaaag 
aaacgcctg aaaaaaaat cgagtgtca aaaaacaaa 
acggtaccg ctccatcca aaatagagc aggaaatta 
aaagtgaaa ctctgtcat ctaacagtt tttatagcc 
aacctggca ctagtaaag tttcggaaa gtacaaaaa 
tcgaaaata aagtagagg cagttcagt tatccttgc 
ctgaaaaat acaagccac agacacttc gagcagtaa 
taggacaac aaggtgatt caagtaatt tggccaaac 
tcaaacact ggcttgaaa attggtaca aaaatcaag 
acccgaaag ttaaaaaaa cattagcac gtcctagtc 
cttggtcta ctaagaaag atgatggtg cttatttca 
agtgtgcgt tgttgagtg gtccaccag gtgttggta 
aaaccacaa ctgcaacac ttgtggcaa aggaattgg 
gtttggata tagtcgagt ttaatgctt ctgatacga 
ggagcaaga agttgctac atgaagaag tggcgcagt 
tgttgagca ctaaaacaa tagctggat atgcaacag 
gccaaccga atgtgaacc gcaaccgcg tgttgctaa 
tggacgaag tggacggaa tggcaggaa acgaggacc 
gcggcggca tccaggaac taatccagc tgattaaaa 
attcaagcg ttccgataa tttgcatgt gcaacgacc 
gaaatcacc aaaaaatcc gatcacttg tcaactact 
gcttcgatt tgaaattta ccaaaccaa aacttgaac 
aaattcggg gggcaatga tgtcgatct gttgcaaag 
aaaacatcg atgtttcca cacaagcat tgactgaaa 
ttatcgccg gaacgggct gtgatgtcc gccagacgc 
tcaatcatt tggctctgt tgggatcaa ccaaagggg 
aaatcacag ttgaagtgg ctgaaaagg agtcaaaag 
cttcaaaga aagacactg tgatgggtc catgggagg 
tgtgtcgga ctgttttta ccaaaagcg aacataagg 
atatgagtg tagctgaca gagcgagat tgtttttct 
tcgattata gtttagggc cgcttttta ttcaagaaa 
attatttga gggtgcaac ccgagggtt caaagaaag 
aagggttac taaaggctg ctttaaccg ctgattcta 
taagtgttg gcgatatta tagaatcaa aaatcagaa 
gaaccaata attgggcgt tgcttgaat cgcaagctt 
tcttttcgt ctgttattc caggtcatt acatgtccg 
gtcatttca attcccaaa ttaatttcc ccgcttggt 
tggggaaaa attcgaaga aaaataagt tcaagaggt 
tgattagtg agctgcaat cgcacacac gtactagtg 
catctgcga gcaaaatgg cagttaagt tggattatt 
tggtaccgc tccgaaatg caattttta caccactga 
ttaagaacg gattggaag gaatcaatc aagcggttg 
gtgtgatga aggattata acctcctga gagaagatg 
tcgatagtt tgatagaac tgtgccaat ggttacaag 
gagaaaatc caatgaata gtatcgatt cgaaagtaa 
aaagtgcgt ttactcgca catacaata aacaagtca 
ctctattac catatgctc ctgcgtctg gagtttcta 
aaaagaaag cggctgctt tggaaagtg atctcttgg 
acgaacttg atgaaattt ccgataatg aggatgatg 
acgatatta gtaaaaatg ctatgatta aggtgaaaa 
agaagacag ctcctaaag gtaagaagg ctgatgaag 
gtaaaggta caagtaaaa atgagaaga aaggaaaag 
cgaagaaaa agtaatttg tgttttctt tttaattat 
tcatttatt tttctctac tatgtttta tttaacacc 
aacaaataa tttctttga aatgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY