| General information | |||
| TC013927 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 2541369..2547392 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013927 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02229 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
- >TC013927 RA
MSKDIRSFF TVISKKSDA NSPKAASPA VKKKPKRII DSDDEDVIS ATPDKEQKK VEKKREVLS SDSDEDAKK KRKTEEPKL KPVNVADVF KNSPIKQSK VEVPQTPEV KPTKAEEKK PKNRKKKHD AEKEIHNNS AFNKTLEDL DDDIFEQSI HLIESIEEA YQGDKEKKE SKNESDSQV QSAKKRSRN DSSSDNQTP KKKKIEHSD SGIDPGQEA HEKKRYSAM LYQRYLNRG EPKHLGMKE LPKGKPNCL QNLCFLRTG VLDSLDSEE FANIIKQHG GRVVHAVSK KVNYVVVGA EPGPAKLAK AESYNIPNI SEDELLDMI LTKSGMEPK YCKKNTSCD SEDLGIDLN HETKPSKEK ISNKTEPKK ETPEKKSSV KKQNGTAPS KIEQEIKSE TLSSNSFYS QPGTSKVSE STKIENKVE AVQLSLPEK YKPQTLRAV IGQQGDSSN LAKLKHWLE NWYKNQDPK VKKTLARPS PWSTKKDDG AYFKCALLS GPPGVGKTT TATLVAKEL GLDIVEFNA SDTRSKKLL HEEVAQLLS TKTIAGYAT GQPNVNRNR VLLMDEVDG MAGNEDRGG IQELIQLIK NSSVPIICM CNDRNHQKI RSLVNYCFD LKFTKPKLE QIRGAMMSI CCKENIDVS TQALTEIIA GTGCDVRQT LNHLALLGS TKGEITVEV AEKESKASK KDTVMGPWE VCRTVFTKS EHKDMSVAD RARLFFFDY SLGPLFIQE NYLRVQPEG SKKEGLLKA ALTADSISV GDIIESKIR RTNNWALLE SQAFFSSVI PGHYMSGHF NSQINFPAW LGKNSKKNK FKRLISELQ SHTRTSASA SKMAVKLDY LVPLRNAIF TPLIKNGLE GINQAVGVM KDYNLLRED VDSLIELCQ WLQGENPMN SIDSKVKSA FTRTYNKQV TLLPYAPAS GVSKKKAAA LESDLLDEL DEISDNEDD DDISKNAMI KVKKKTAPK GKKADEGKG TSKNEKKGK AKKK
- >TC013927 RA
atgtcgaaa gacatccgt tcctttttc actgtaatt tcaaagaag tctgatgca aattcgccc aaagctgct tctcctgcc gttaaaaag aagccaaaa cgtatcata gactccgat gatgaggac gtgatttct gccactcca gacaaagag cagaagaag gttgagaag aaacgtgaa gttctaagt tcagactct gatgaagac gcgaagaaa aaaaggaag actgaagaa ccgaaatta aaaccggtg aatgtggcc gacgtgttt aagaacagt cctataaaa cagagcaag gttgaagtg ccacaaaca cccgaagtc aagcctaca aaagcagaa gagaaaaaa cccaaaaat aggaagaaa aagcacgat gcggagaag gagattcac aacaatagt gctttcaac aagacgctg gaagactta gatgatgat attttcgaa cagagtatt catcttatt gagtcaatt gaggaagcg taccagggt gataaagaa aagaaagaa agtaaaaat gaatcagat agtcaagtt caaagcgca aagaaacga tcaagaaac gattcgtca agtgataat caaacacct aagaagaag aaaattgag cattcggac tctggtatt gatccggga caagaagct catgaaaaa aagagatac tcagccatg ctctaccaa aggtacctc aacagaggg gaacccaaa catcttgga atgaaggaa ttgccaaaa gggaagccg aattgtttg caaaatttg tgtttccta agaactggc gttttagac tcgcttgat tcggaggag tttgcaaat ataataaaa caacacggt ggacgtgta gtgcacgca gtttcaaaa aaagtaaat tatgtggtt gtgggtgca gaaccaggg ccggcaaaa cttgcaaaa gctgaaagt tataacata cctaacata tctgaagat gagcttttg gacatgata cttacaaag tcagggatg gaaccgaaa tattgcaaa aagaatacg tcgtgtgat agcgaggat ttaggcatt gatttaaac catgaaaca aaacctagc aaagaaaaa ataagtaac aaaactgag cctaaaaaa gaaacgcct gaaaaaaaa tcgagtgtc aaaaaacaa aacggtacc gctccatcc aaaatagag caggaaatt aaaagtgaa actctgtca tctaacagt ttttatagc caacctggc actagtaaa gtttcggaa agtacaaaa atcgaaaat aaagtagag gcagttcag ttatccttg cctgaaaaa tacaagcca cagacactt cgagcagta ataggacaa caaggtgat tcaagtaat ttggccaaa ctcaaacac tggcttgaa aattggtac aaaaatcaa gacccgaaa gttaaaaaa acattagca cgtcctagt ccttggtct actaagaaa gatgatggt gcttatttc aagtgtgcg ttgttgagt ggtccacca ggtgttggt aaaaccaca actgcaaca cttgtggca aaggaattg ggtttggat atagtcgag tttaatgct tctgatacg aggagcaag aagttgcta catgaagaa gtggcgcag ttgttgagc actaaaaca atagctgga tatgcaaca ggccaaccg aatgtgaac cgcaaccgc gtgttgcta atggacgaa gtggacgga atggcagga aacgaggac cgcggcggc atccaggaa ctaatccag ctgattaaa aattcaagc gttccgata atttgcatg tgcaacgac cgaaatcac caaaaaatc cgatcactt gtcaactac tgcttcgat ttgaaattt accaaacca aaacttgaa caaattcgg ggggcaatg atgtcgatc tgttgcaaa gaaaacatc gatgtttcc acacaagca ttgactgaa attatcgcc ggaacgggc tgtgatgtc cgccagacg ctcaatcat ttggctctg ttgggatca accaaaggg gaaatcaca gttgaagtg gctgaaaag gagtcaaaa gcttcaaag aaagacact gtgatgggt ccatgggag gtgtgtcgg actgttttt accaaaagc gaacataag gatatgagt gtagctgac agagcgaga ttgtttttc ttcgattat agtttaggg ccgcttttt attcaagaa aattatttg agggtgcaa cccgagggt tcaaagaaa gaagggtta ctaaaggct gctttaacc gctgattct ataagtgtt ggcgatatt atagaatca aaaatcaga agaaccaat aattgggcg ttgcttgaa tcgcaagct ttcttttcg tctgttatt ccaggtcat tacatgtcc ggtcatttc aattcccaa attaatttc cccgcttgg ttggggaaa aattcgaag aaaaataag ttcaagagg ttgattagt gagctgcaa tcgcacaca cgtactagt gcatctgcg agcaaaatg gcagttaag ttggattat ttggtaccg ctccgaaat gcaattttt acaccactg attaagaac ggattggaa ggaatcaat caagcggtt ggtgtgatg aaggattat aacctcctg agagaagat gtcgatagt ttgatagaa ctgtgccaa tggttacaa ggagaaaat ccaatgaat agtatcgat tcgaaagta aaaagtgcg tttactcgc acatacaat aaacaagtc actctatta ccatatgct cctgcgtct ggagtttct aaaaagaaa gcggctgct ttggaaagt gatctcttg gacgaactt gatgaaatt tccgataat gaggatgat gacgatatt agtaaaaat gctatgatt aaggtgaaa aagaagaca gctcctaaa ggtaagaag gctgatgaa ggtaaaggt acaagtaaa aatgagaag aaaggaaaa gcgaagaaa aagtaa
- >TC013927 RA
tgtgaatta aacaacaac ctgcaaaca gataataat ttgataaaa acaacagat aattttggg tgtaacgta atcgtacca tgtcgaaag acatccgtt cctttttca ctgtaattt caaagaagt ctgatgcaa attcgccca aagctgctt ctcctgccg ttaaaaaga agccaaaac gtatcatag actccgatg atgaggacg tgatttctg ccactccag acaaagagc agaagaagg ttgagaaga aacgtgaag ttctaagtt cagactctg atgaagacg cgaagaaaa aaaggaaga ctgaagaac cgaaattaa aaccggtga atgtggccg acgtgttta agaacagtc ctataaaac agagcaagg ttgaagtgc cacaaacac ccgaagtca agcctacaa aagcagaag agaaaaaac ccaaaaata ggaagaaaa agcacgatg cggagaagg agattcaca acaatagtg ctttcaaca agacgctgg aagacttag atgatgata ttttcgaac agagtattc atcttattg agtcaattg aggaagcgt accagggtg ataaagaaa agaaagaaa gtaaaaatg aatcagata gtcaagttc aaagcgcaa agaaacgat caagaaacg attcgtcaa gtgataatc aaacaccta agaagaaga aaattgagc attcggact ctggtattg atccgggac aagaagctc atgaaaaaa agagatact cagccatgc tctaccaaa ggtacctca acagagggg aacccaaac atcttggaa tgaaggaat tgccaaaag ggaagccga attgtttgc aaaatttgt gtttcctaa gaactggcg ttttagact cgcttgatt cggaggagt ttgcaaata taataaaac aacacggtg gacgtgtag tgcacgcag tttcaaaaa aagtaaatt atgtggttg tgggtgcag aaccagggc cggcaaaac ttgcaaaag ctgaaagtt ataacatac ctaacatat ctgaagatg agcttttgg acatgatac ttacaaagt cagggatgg aaccgaaat attgcaaaa agaatacgt cgtgtgata gcgaggatt taggcattg atttaaacc atgaaacaa aacctagca aagaaaaaa taagtaaca aaactgagc ctaaaaaag aaacgcctg aaaaaaaat cgagtgtca aaaaacaaa acggtaccg ctccatcca aaatagagc aggaaatta aaagtgaaa ctctgtcat ctaacagtt tttatagcc aacctggca ctagtaaag tttcggaaa gtacaaaaa tcgaaaata aagtagagg cagttcagt tatccttgc ctgaaaaat acaagccac agacacttc gagcagtaa taggacaac aaggtgatt caagtaatt tggccaaac tcaaacact ggcttgaaa attggtaca aaaatcaag acccgaaag ttaaaaaaa cattagcac gtcctagtc cttggtcta ctaagaaag atgatggtg cttatttca agtgtgcgt tgttgagtg gtccaccag gtgttggta aaaccacaa ctgcaacac ttgtggcaa aggaattgg gtttggata tagtcgagt ttaatgctt ctgatacga ggagcaaga agttgctac atgaagaag tggcgcagt tgttgagca ctaaaacaa tagctggat atgcaacag gccaaccga atgtgaacc gcaaccgcg tgttgctaa tggacgaag tggacggaa tggcaggaa acgaggacc gcggcggca tccaggaac taatccagc tgattaaaa attcaagcg ttccgataa tttgcatgt gcaacgacc gaaatcacc aaaaaatcc gatcacttg tcaactact gcttcgatt tgaaattta ccaaaccaa aacttgaac aaattcggg gggcaatga tgtcgatct gttgcaaag aaaacatcg atgtttcca cacaagcat tgactgaaa ttatcgccg gaacgggct gtgatgtcc gccagacgc tcaatcatt tggctctgt tgggatcaa ccaaagggg aaatcacag ttgaagtgg ctgaaaagg agtcaaaag cttcaaaga aagacactg tgatgggtc catgggagg tgtgtcgga ctgttttta ccaaaagcg aacataagg atatgagtg tagctgaca gagcgagat tgtttttct tcgattata gtttagggc cgcttttta ttcaagaaa attatttga gggtgcaac ccgagggtt caaagaaag aagggttac taaaggctg ctttaaccg ctgattcta taagtgttg gcgatatta tagaatcaa aaatcagaa gaaccaata attgggcgt tgcttgaat cgcaagctt tcttttcgt ctgttattc caggtcatt acatgtccg gtcatttca attcccaaa ttaatttcc ccgcttggt tggggaaaa attcgaaga aaaataagt tcaagaggt tgattagtg agctgcaat cgcacacac gtactagtg catctgcga gcaaaatgg cagttaagt tggattatt tggtaccgc tccgaaatg caattttta caccactga ttaagaacg gattggaag gaatcaatc aagcggttg gtgtgatga aggattata acctcctga gagaagatg tcgatagtt tgatagaac tgtgccaat ggttacaag gagaaaatc caatgaata gtatcgatt cgaaagtaa aaagtgcgt ttactcgca catacaata aacaagtca ctctattac catatgctc ctgcgtctg gagtttcta aaaagaaag cggctgctt tggaaagtg atctcttgg acgaacttg atgaaattt ccgataatg aggatgatg acgatatta gtaaaaatg ctatgatta aggtgaaaa agaagacag ctcctaaag gtaagaagg ctgatgaag gtaaaggta caagtaaaa atgagaaga aaggaaaag cgaagaaaa agtaatttg tgttttctt tttaattat tcatttatt tttctctac tatgtttta tttaacacc aacaaataa tttctttga aatgaa
