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Gene information
General information
TC013927 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2541369..2547392
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013927
    iBeetle screen iB_02229

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013927 RA
      MSKDIRSFF TVISKKSDA NSPKAASPA VKKKPKRII 
      DSDDEDVIS ATPDKEQKK VEKKREVLS SDSDEDAKK 
      KRKTEEPKL KPVNVADVF KNSPIKQSK VEVPQTPEV 
      KPTKAEEKK PKNRKKKHD AEKEIHNNS AFNKTLEDL 
      DDDIFEQSI HLIESIEEA YQGDKEKKE SKNESDSQV 
      QSAKKRSRN DSSSDNQTP KKKKIEHSD SGIDPGQEA 
      HEKKRYSAM LYQRYLNRG EPKHLGMKE LPKGKPNCL 
      QNLCFLRTG VLDSLDSEE FANIIKQHG GRVVHAVSK 
      KVNYVVVGA EPGPAKLAK AESYNIPNI SEDELLDMI 
      LTKSGMEPK YCKKNTSCD SEDLGIDLN HETKPSKEK 
      ISNKTEPKK ETPEKKSSV KKQNGTAPS KIEQEIKSE 
      TLSSNSFYS QPGTSKVSE STKIENKVE AVQLSLPEK 
      YKPQTLRAV IGQQGDSSN LAKLKHWLE NWYKNQDPK 
      VKKTLARPS PWSTKKDDG AYFKCALLS GPPGVGKTT 
      TATLVAKEL GLDIVEFNA SDTRSKKLL HEEVAQLLS 
      TKTIAGYAT GQPNVNRNR VLLMDEVDG MAGNEDRGG 
      IQELIQLIK NSSVPIICM CNDRNHQKI RSLVNYCFD 
      LKFTKPKLE QIRGAMMSI CCKENIDVS TQALTEIIA 
      GTGCDVRQT LNHLALLGS TKGEITVEV AEKESKASK 
      KDTVMGPWE VCRTVFTKS EHKDMSVAD RARLFFFDY 
      SLGPLFIQE NYLRVQPEG SKKEGLLKA ALTADSISV 
      GDIIESKIR RTNNWALLE SQAFFSSVI PGHYMSGHF 
      NSQINFPAW LGKNSKKNK FKRLISELQ SHTRTSASA 
      SKMAVKLDY LVPLRNAIF TPLIKNGLE GINQAVGVM 
      KDYNLLRED VDSLIELCQ WLQGENPMN SIDSKVKSA 
      FTRTYNKQV TLLPYAPAS GVSKKKAAA LESDLLDEL 
      DEISDNEDD DDISKNAMI KVKKKTAPK GKKADEGKG 
      TSKNEKKGK AKKK
    CDS sequence
    • >TC013927 RA
      atgtcgaaa gacatccgt tcctttttc actgtaatt 
      tcaaagaag tctgatgca aattcgccc aaagctgct 
      tctcctgcc gttaaaaag aagccaaaa cgtatcata 
      gactccgat gatgaggac gtgatttct gccactcca 
      gacaaagag cagaagaag gttgagaag aaacgtgaa 
      gttctaagt tcagactct gatgaagac gcgaagaaa 
      aaaaggaag actgaagaa ccgaaatta aaaccggtg 
      aatgtggcc gacgtgttt aagaacagt cctataaaa 
      cagagcaag gttgaagtg ccacaaaca cccgaagtc 
      aagcctaca aaagcagaa gagaaaaaa cccaaaaat 
      aggaagaaa aagcacgat gcggagaag gagattcac 
      aacaatagt gctttcaac aagacgctg gaagactta 
      gatgatgat attttcgaa cagagtatt catcttatt 
      gagtcaatt gaggaagcg taccagggt gataaagaa 
      aagaaagaa agtaaaaat gaatcagat agtcaagtt 
      caaagcgca aagaaacga tcaagaaac gattcgtca 
      agtgataat caaacacct aagaagaag aaaattgag 
      cattcggac tctggtatt gatccggga caagaagct 
      catgaaaaa aagagatac tcagccatg ctctaccaa 
      aggtacctc aacagaggg gaacccaaa catcttgga 
      atgaaggaa ttgccaaaa gggaagccg aattgtttg 
      caaaatttg tgtttccta agaactggc gttttagac 
      tcgcttgat tcggaggag tttgcaaat ataataaaa 
      caacacggt ggacgtgta gtgcacgca gtttcaaaa 
      aaagtaaat tatgtggtt gtgggtgca gaaccaggg 
      ccggcaaaa cttgcaaaa gctgaaagt tataacata 
      cctaacata tctgaagat gagcttttg gacatgata 
      cttacaaag tcagggatg gaaccgaaa tattgcaaa 
      aagaatacg tcgtgtgat agcgaggat ttaggcatt 
      gatttaaac catgaaaca aaacctagc aaagaaaaa 
      ataagtaac aaaactgag cctaaaaaa gaaacgcct 
      gaaaaaaaa tcgagtgtc aaaaaacaa aacggtacc 
      gctccatcc aaaatagag caggaaatt aaaagtgaa 
      actctgtca tctaacagt ttttatagc caacctggc 
      actagtaaa gtttcggaa agtacaaaa atcgaaaat 
      aaagtagag gcagttcag ttatccttg cctgaaaaa 
      tacaagcca cagacactt cgagcagta ataggacaa 
      caaggtgat tcaagtaat ttggccaaa ctcaaacac 
      tggcttgaa aattggtac aaaaatcaa gacccgaaa 
      gttaaaaaa acattagca cgtcctagt ccttggtct 
      actaagaaa gatgatggt gcttatttc aagtgtgcg 
      ttgttgagt ggtccacca ggtgttggt aaaaccaca 
      actgcaaca cttgtggca aaggaattg ggtttggat 
      atagtcgag tttaatgct tctgatacg aggagcaag 
      aagttgcta catgaagaa gtggcgcag ttgttgagc 
      actaaaaca atagctgga tatgcaaca ggccaaccg 
      aatgtgaac cgcaaccgc gtgttgcta atggacgaa 
      gtggacgga atggcagga aacgaggac cgcggcggc 
      atccaggaa ctaatccag ctgattaaa aattcaagc 
      gttccgata atttgcatg tgcaacgac cgaaatcac 
      caaaaaatc cgatcactt gtcaactac tgcttcgat 
      ttgaaattt accaaacca aaacttgaa caaattcgg 
      ggggcaatg atgtcgatc tgttgcaaa gaaaacatc 
      gatgtttcc acacaagca ttgactgaa attatcgcc 
      ggaacgggc tgtgatgtc cgccagacg ctcaatcat 
      ttggctctg ttgggatca accaaaggg gaaatcaca 
      gttgaagtg gctgaaaag gagtcaaaa gcttcaaag 
      aaagacact gtgatgggt ccatgggag gtgtgtcgg 
      actgttttt accaaaagc gaacataag gatatgagt 
      gtagctgac agagcgaga ttgtttttc ttcgattat 
      agtttaggg ccgcttttt attcaagaa aattatttg 
      agggtgcaa cccgagggt tcaaagaaa gaagggtta 
      ctaaaggct gctttaacc gctgattct ataagtgtt 
      ggcgatatt atagaatca aaaatcaga agaaccaat 
      aattgggcg ttgcttgaa tcgcaagct ttcttttcg 
      tctgttatt ccaggtcat tacatgtcc ggtcatttc 
      aattcccaa attaatttc cccgcttgg ttggggaaa 
      aattcgaag aaaaataag ttcaagagg ttgattagt 
      gagctgcaa tcgcacaca cgtactagt gcatctgcg 
      agcaaaatg gcagttaag ttggattat ttggtaccg 
      ctccgaaat gcaattttt acaccactg attaagaac 
      ggattggaa ggaatcaat caagcggtt ggtgtgatg 
      aaggattat aacctcctg agagaagat gtcgatagt 
      ttgatagaa ctgtgccaa tggttacaa ggagaaaat 
      ccaatgaat agtatcgat tcgaaagta aaaagtgcg 
      tttactcgc acatacaat aaacaagtc actctatta 
      ccatatgct cctgcgtct ggagtttct aaaaagaaa 
      gcggctgct ttggaaagt gatctcttg gacgaactt 
      gatgaaatt tccgataat gaggatgat gacgatatt 
      agtaaaaat gctatgatt aaggtgaaa aagaagaca 
      gctcctaaa ggtaagaag gctgatgaa ggtaaaggt 
      acaagtaaa aatgagaag aaaggaaaa gcgaagaaa 
      aagtaa
    mRNA sequence
    • >TC013927 RA
      tgtgaatta aacaacaac ctgcaaaca gataataat 
      ttgataaaa acaacagat aattttggg tgtaacgta 
      atcgtacca tgtcgaaag acatccgtt cctttttca 
      ctgtaattt caaagaagt ctgatgcaa attcgccca 
      aagctgctt ctcctgccg ttaaaaaga agccaaaac 
      gtatcatag actccgatg atgaggacg tgatttctg 
      ccactccag acaaagagc agaagaagg ttgagaaga 
      aacgtgaag ttctaagtt cagactctg atgaagacg 
      cgaagaaaa aaaggaaga ctgaagaac cgaaattaa 
      aaccggtga atgtggccg acgtgttta agaacagtc 
      ctataaaac agagcaagg ttgaagtgc cacaaacac 
      ccgaagtca agcctacaa aagcagaag agaaaaaac 
      ccaaaaata ggaagaaaa agcacgatg cggagaagg 
      agattcaca acaatagtg ctttcaaca agacgctgg 
      aagacttag atgatgata ttttcgaac agagtattc 
      atcttattg agtcaattg aggaagcgt accagggtg 
      ataaagaaa agaaagaaa gtaaaaatg aatcagata 
      gtcaagttc aaagcgcaa agaaacgat caagaaacg 
      attcgtcaa gtgataatc aaacaccta agaagaaga 
      aaattgagc attcggact ctggtattg atccgggac 
      aagaagctc atgaaaaaa agagatact cagccatgc 
      tctaccaaa ggtacctca acagagggg aacccaaac 
      atcttggaa tgaaggaat tgccaaaag ggaagccga 
      attgtttgc aaaatttgt gtttcctaa gaactggcg 
      ttttagact cgcttgatt cggaggagt ttgcaaata 
      taataaaac aacacggtg gacgtgtag tgcacgcag 
      tttcaaaaa aagtaaatt atgtggttg tgggtgcag 
      aaccagggc cggcaaaac ttgcaaaag ctgaaagtt 
      ataacatac ctaacatat ctgaagatg agcttttgg 
      acatgatac ttacaaagt cagggatgg aaccgaaat 
      attgcaaaa agaatacgt cgtgtgata gcgaggatt 
      taggcattg atttaaacc atgaaacaa aacctagca 
      aagaaaaaa taagtaaca aaactgagc ctaaaaaag 
      aaacgcctg aaaaaaaat cgagtgtca aaaaacaaa 
      acggtaccg ctccatcca aaatagagc aggaaatta 
      aaagtgaaa ctctgtcat ctaacagtt tttatagcc 
      aacctggca ctagtaaag tttcggaaa gtacaaaaa 
      tcgaaaata aagtagagg cagttcagt tatccttgc 
      ctgaaaaat acaagccac agacacttc gagcagtaa 
      taggacaac aaggtgatt caagtaatt tggccaaac 
      tcaaacact ggcttgaaa attggtaca aaaatcaag 
      acccgaaag ttaaaaaaa cattagcac gtcctagtc 
      cttggtcta ctaagaaag atgatggtg cttatttca 
      agtgtgcgt tgttgagtg gtccaccag gtgttggta 
      aaaccacaa ctgcaacac ttgtggcaa aggaattgg 
      gtttggata tagtcgagt ttaatgctt ctgatacga 
      ggagcaaga agttgctac atgaagaag tggcgcagt 
      tgttgagca ctaaaacaa tagctggat atgcaacag 
      gccaaccga atgtgaacc gcaaccgcg tgttgctaa 
      tggacgaag tggacggaa tggcaggaa acgaggacc 
      gcggcggca tccaggaac taatccagc tgattaaaa 
      attcaagcg ttccgataa tttgcatgt gcaacgacc 
      gaaatcacc aaaaaatcc gatcacttg tcaactact 
      gcttcgatt tgaaattta ccaaaccaa aacttgaac 
      aaattcggg gggcaatga tgtcgatct gttgcaaag 
      aaaacatcg atgtttcca cacaagcat tgactgaaa 
      ttatcgccg gaacgggct gtgatgtcc gccagacgc 
      tcaatcatt tggctctgt tgggatcaa ccaaagggg 
      aaatcacag ttgaagtgg ctgaaaagg agtcaaaag 
      cttcaaaga aagacactg tgatgggtc catgggagg 
      tgtgtcgga ctgttttta ccaaaagcg aacataagg 
      atatgagtg tagctgaca gagcgagat tgtttttct 
      tcgattata gtttagggc cgcttttta ttcaagaaa 
      attatttga gggtgcaac ccgagggtt caaagaaag 
      aagggttac taaaggctg ctttaaccg ctgattcta 
      taagtgttg gcgatatta tagaatcaa aaatcagaa 
      gaaccaata attgggcgt tgcttgaat cgcaagctt 
      tcttttcgt ctgttattc caggtcatt acatgtccg 
      gtcatttca attcccaaa ttaatttcc ccgcttggt 
      tggggaaaa attcgaaga aaaataagt tcaagaggt 
      tgattagtg agctgcaat cgcacacac gtactagtg 
      catctgcga gcaaaatgg cagttaagt tggattatt 
      tggtaccgc tccgaaatg caattttta caccactga 
      ttaagaacg gattggaag gaatcaatc aagcggttg 
      gtgtgatga aggattata acctcctga gagaagatg 
      tcgatagtt tgatagaac tgtgccaat ggttacaag 
      gagaaaatc caatgaata gtatcgatt cgaaagtaa 
      aaagtgcgt ttactcgca catacaata aacaagtca 
      ctctattac catatgctc ctgcgtctg gagtttcta 
      aaaagaaag cggctgctt tggaaagtg atctcttgg 
      acgaacttg atgaaattt ccgataatg aggatgatg 
      acgatatta gtaaaaatg ctatgatta aggtgaaaa 
      agaagacag ctcctaaag gtaagaagg ctgatgaag 
      gtaaaggta caagtaaaa atgagaaga aaggaaaag 
      cgaagaaaa agtaatttg tgttttctt tttaattat 
      tcatttatt tttctctac tatgtttta tttaacacc 
      aacaaataa tttctttga aatgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY