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Gene information
General information
TC013924 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2124874..2131648
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013924
    iBeetle screen iB_02228

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013924 RA
      MDNSSSETD VIEQIENDE FYSCFLKTG SKNAIPQAL 
      SISEQVKKL GEGIELISR ELQKQVLEK HDDLLRQGN 
      HATKLENVL NTMNGHVQN LFANAERLR NQVTGPYNA 
      LEMHTKVLG RLHLASHIL RQVNRIQQL SKRLANTND 
      PVQKATILH ELEQLASDP DLKDIDAVT SELRNIRAQ 
      QQKVVKLAT GSLNQGIIN ENVAQTTTA LQIFINLGT 
      IDATLHTTI ENNFSECRE CLKTAFDAS IVSSGLKTT 
      KGPGRATLT TSQGFRNKI WSEIEKAFS EEIYQNCKQ 
      IKFLQTTVN NLNIQNIDS DIATKFWTK LGNLLKSEI 
      KNTSSAVQQ MLEEDYPKL LKCFYEMCE KLKYDQFVF 
      DRQVLEKCE NSYLSSSLN RLLDPAQSM FSSEGEVPS 
      HDQIDSLIR VISTELSVA LIEETLSEK ICKNVSKSI 
      KMFALKTEQ QIETGPEAA QVIGGNANS GQQRNIQFA 
      NALYYFQVQ IQRMLSNMK DSLPQSGIT IINESLQVL 
      VNLVGAIIQ PIVVSINST IETIIITIH LETDWAKLQ 
      VPINKNSVV CSPYMRELS QFVTRVYHT YLSSFNNKE 
      VLLEKCSEI AVRCIELLV RHSSLLRPI SQGGRVRLQ 
      SDYLHLEGA LRVICPHLS DLGRPYRLL KSMASLVTL 
      TPEEIVASQ ASESSVPHS TVLLLLFSF AGSELASPH 
      QNTAWSLPK LSAWLDEHP SESDRLDLI VGALQKYEN 
      LVRQKNSVN YDPVYPIMS TFLENAVKT VK
    CDS sequence
    • >TC013924 RA
      atggataat tccagctca gaaacagat gtaatagaa 
      cagattgaa aacgacgag ttttatagt tgtttcttg 
      aagaccggc tccaaaaat gccattcct caagcccta 
      agtatttct gaacaagtc aaaaagctt ggcgaaggc 
      atcgaatta atcagtcgc gaattacaa aagcaagtt 
      ttggaaaaa cacgatgat ttactcaga cagggcaac 
      catgcaact aaattggag aacgtctta aatacgatg 
      aatggccac gtccagaat ttatttgct aatgctgaa 
      cgtttgagg aatcaagtg acaggtcca tacaatgcg 
      ctagaaatg cacacgaaa gtattgggc cgtttgcat 
      ttggccagt catatcctg agacaggtt aatagaatc 
      cagcagttg agtaaacga ctggctaat actaacgat 
      cctgtccag aaagcaaca attttgcac gagttagag 
      caactagcg tcagatccg gatttaaag gacattgat 
      gccgtaaca tcagaattg cgaaatata agagcccaa 
      caacagaaa gttgtcaaa ttggccaca ggatcctta 
      aatcaagga ataatcaat gaaaatgtg gcccaaacc 
      accactgct ttacaaatt tttattaat ttggggact 
      attgatgcc accttacac acaactatt gaaaataat 
      ttcagcgag tgtcgtgaa tgtttaaaa acggctttt 
      gacgccagt attgtctca agtgggctt aaaacaacc 
      aaagggcct ggaagggcc actttaacc acttcgcaa 
      ggcttcaga aataaaatt tggagtgag attgagaaa 
      gcattctct gaggaaatc taccaaaat tgcaagcag 
      atcaagttt ttgcaaaca accgtaaac aacttaaac 
      atccaaaat atcgacagt gacatcgct acgaaattt 
      tggacgaaa ttaggcaat ttgttgaag agtgaaatc 
      aaaaacacg tcttcagca gttcagcaa atgttggaa 
      gaggactat ccaaaatta ctgaagtgc ttttacgaa 
      atgtgcgag aaactgaaa tacgatcag tttgttttt 
      gaccgccaa gtgctcgaa aagtgtgaa aattcgtat 
      ttgtcaagc tcattaaac agattattg gatccagct 
      cagtctatg tttagtagt gaaggggaa gtgccctct 
      cacgaccag attgattcc ttaattcgg gtcattagc 
      accgagtta agtgttgcc ttgattgag gaaacgtta 
      agtgagaaa atttgtaaa aacgtgtcc aaatctatc 
      aaaatgttt gcgctaaaa accgaacaa cagattgaa 
      actggacca gaagcggct caggtcatt ggaggcaat 
      gctaactca ggacagcaa aggaacatc caatttgca 
      aacgccttg tattatttt caagtacaa atccagaga 
      atgttgtca aacatgaaa gacagtttg ccacagtct 
      ggtattacg attattaac gaaagttta caagttctt 
      gttaactta gttggggct ataattcag cctattgtt 
      gtctcaata aactcaaca attgaaacg ataatcata 
      acaattcat cttgaaaca gattgggcc aagcttcaa 
      gtcccgata aataaaaat tcagtggtt tgtagcccc 
      tacatgcga gagttgtct cagtttgtc actcgtgtt 
      tatcacacc tatttatca agttttaac aataaagaa 
      gtattgctt gaaaaatgt agcgaaatc gccgtccgt 
      tgcattgag ctcctggtc cgccactcc tccttactc 
      cggcccata tcacaaggg gggcgcgtg cgactccag 
      tccgactac ctccatctc gaaggcgcc ctcagggtg 
      atttgcccc catttgagc gatttgggg cgtccttat 
      cgtttacta aaatccatg gcgtctctt gtcacccta 
      actccagag gaaatcgtc gcaagtcaa gcttctgaa 
      agctcagtg cctcacagt accgtttta cttttatta 
      ttttcattt gccggaagt gaattggcg agtccgcac 
      caaaacaca gcgtggtca cttccgaaa ttatcggcc 
      tggttggat gagcaccct tcggaatcg gatcggttg 
      gatttgatc gtgggagcc ctccaaaag tacgaaaat 
      ctagtgagg cagaaaaat tccgttaat tatgacccg 
      gtttatccg atcatgtct acgtttttg gaaaatgcg 
      gttaaaacg gttaaataa
    mRNA sequence
    • >TC013924 RA
      gatataaag tttgtgatg tgtcaaacc cttgtcaat 
      aaaagttta tgaattatt ttaattaca caaaaacag 
      tggtaataa tggataatt ccagctcag aaacagatg 
      taatagaac agattgaaa acgacgagt tttatagtt 
      gtttcttga agaccggct ccaaaaatg ccattcctc 
      aagccctaa gtatttctg aacaagtca aaaagcttg 
      gcgaaggca tcgaattaa tcagtcgcg aattacaaa 
      agcaagttt tggaaaaac acgatgatt tactcagac 
      agggcaacc atgcaacta aattggaga acgtcttaa 
      atacgatga atggccacg tccagaatt tatttgcta 
      atgctgaac gtttgagga atcaagtga caggtccat 
      acaatgcgc tagaaatgc acacgaaag tattgggcc 
      gtttgcatt tggccagtc atatcctga gacaggtta 
      atagaatcc agcagttga gtaaacgac tggctaata 
      ctaacgatc ctgtccaga aagcaacaa ttttgcacg 
      agttagagc aactagcgt cagatccgg atttaaagg 
      acattgatg ccgtaacat cagaattgc gaaatataa 
      gagcccaac aacagaaag ttgtcaaat tggccacag 
      gatccttaa atcaaggaa taatcaatg aaaatgtgg 
      cccaaacca ccactgctt tacaaattt ttattaatt 
      tggggacta ttgatgcca ccttacaca caactattg 
      aaaataatt tcagcgagt gtcgtgaat gtttaaaaa 
      cggcttttg acgccagta ttgtctcaa gtgggctta 
      aaacaacca aagggcctg gaagggcca ctttaacca 
      cttcgcaag gcttcagaa ataaaattt ggagtgaga 
      ttgagaaag cattctctg aggaaatct accaaaatt 
      gcaagcaga tcaagtttt tgcaaacaa ccgtaaaca 
      acttaaaca tccaaaata tcgacagtg acatcgcta 
      cgaaatttt ggacgaaat taggcaatt tgttgaaga 
      gtgaaatca aaaacacgt cttcagcag ttcagcaaa 
      tgttggaag aggactatc caaaattac tgaagtgct 
      tttacgaaa tgtgcgaga aactgaaat acgatcagt 
      ttgtttttg accgccaag tgctcgaaa agtgtgaaa 
      attcgtatt tgtcaagct cattaaaca gattattgg 
      atccagctc agtctatgt ttagtagtg aaggggaag 
      tgccctctc acgaccaga ttgattcct taattcggg 
      tcattagca ccgagttaa gtgttgcct tgattgagg 
      aaacgttaa gtgagaaaa tttgtaaaa acgtgtcca 
      aatctatca aaatgtttg cgctaaaaa ccgaacaac 
      agattgaaa ctggaccag aagcggctc aggtcattg 
      gaggcaatg ctaactcag gacagcaaa ggaacatcc 
      aatttgcaa acgccttgt attattttc aagtacaaa 
      tccagagaa tgttgtcaa acatgaaag acagtttgc 
      cacagtctg gtattacga ttattaacg aaagtttac 
      aagttcttg ttaacttag ttggggcta taattcagc 
      ctattgttg tctcaataa actcaacaa ttgaaacga 
      taatcataa caattcatc ttgaaacag attgggcca 
      agcttcaag tcccgataa ataaaaatt cagtggttt 
      gtagcccct acatgcgag agttgtctc agtttgtca 
      ctcgtgttt atcacacct atttatcaa gttttaaca 
      ataaagaag tattgcttg aaaaatgta gcgaaatcg 
      ccgtccgtt gcattgagc tcctggtcc gccactcct 
      ccttactcc ggcccatat cacaagggg ggcgcgtgc 
      gactccagt ccgactacc tccatctcg aaggcgccc 
      tcagggtga tttgccccc atttgagcg atttggggc 
      gtccttatc gtttactaa aatccatgg cgtctcttg 
      tcaccctaa ctccagagg aaatcgtcg caagtcaag 
      cttctgaaa gctcagtgc ctcacagta ccgttttac 
      ttttattat tttcatttg ccggaagtg aattggcga 
      gtccgcacc aaaacacag cgtggtcac ttccgaaat 
      tatcggcct ggttggatg agcaccctt cggaatcgg 
      atcggttgg atttgatcg tgggagccc tccaaaagt 
      acgaaaatc tagtgaggc agaaaaatt ccgttaatt 
      atgacccgg tttatccga tcatgtcta cgtttttgg 
      aaaatgcgg ttaaaacgg ttaaataaa tattttgtt 
      aaactttga ttttgtttt cctgattac ggtcctgta 
      ttcaattgc ttggca
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