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Gene information
General information
TC013923 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2131890..2134358
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013923
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02227

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4

    egg chamber number decreased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    iBeetle screen iB_10662

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013923 RA
      MFKIFLNYL KFSANCTLN NNSKALGHF NNSIRRYAT 
      RKRFYRNTG ILRSEGKYE VTLDQRKLK TPKGNLFSV 
      ESEPLAIAV ATEWDSQKE NIIQSKMHL TTLCNTVID 
      NPNNLTKYD MVDYITNYL DTDTILFQA NEEEDLLEL 
      QEKEWDPII DWFNKRFDV SIKKSQQMD APPASEKDK 
      ATLSKHLLS HNFATICGF VYGVDTLKS VILTLACVE 
      HFLTPEKAV LLSRLEEEY QTGHWGRVE WAHDLNQQD 
      LQARLAAVI LFTYFNNQS SKIKSKSIQ
    CDS sequence
    • >TC013923 RA
      atgttcaaa atattttta aattattta aaattcagt 
      gccaattgt acactaaat aataattca aaagcacta 
      gggcatttt aacaattca atcagacgt tatgcaact 
      cgaaaacgt ttttatcga aacactggg attttacgc 
      agcgaaggc aaatacgaa gtcacgtta gaccagaga 
      aaattaaaa acaccgaaa ggaaattta tttagcgtt 
      gaaagtgaa cccttagct atagcagta gccacagaa 
      tgggattcc caaaaggag aacattatt cagagtaaa 
      atgcacctt acaacctta tgtaacaca gtcattgat 
      aatccgaat aatttaaca aagtacgac atggttgac 
      tacatcact aattatcta gacaccgac acgatttta 
      tttcaagcc aatgaagag gaagatttg ctcgaattg 
      caagaaaaa gagtgggac cctatcata gactggttc 
      aataaacgc tttgatgtg agtataaaa aaatcgcaa 
      caaatggac gcccctcca gccagtgaa aaggacaaa 
      gctacccta agcaaacac ctcttgtca cacaacttt 
      gccacaatt tgcggtttt gtgtacgga gtggatacg 
      ttaaaatct gttatattg acgctagcg tgtgtggaa 
      catttccta acacctgaa aaagcagtt cttttgtct 
      cgactggag gaggagtat cagacgggg cactggggc 
      agagtcgag tgggctcat gatttgaac caacaagac 
      ctgcaagcc aggctagct gcagtcata cttttcacc 
      tactttaac aaccagtcg agtaaaatt aaatcgaaa 
      tcaatccag taa
    mRNA sequence
    • >TC013923 RA
      ctaacctaa aaaatatgt cattgatgt caccatgtt 
      caaaatatt tttaaatta tttaaaatt cagtgccaa 
      ttgtacact aaataataa ttcaaaagc actagggca 
      ttttaacaa ttcaatcag acgttatgc aactcgaaa 
      acgttttta tcgaaacac tgggatttt acgcagcga 
      aggcaaata cgaagtcac gttagacca gagaaaatt 
      aaaaacacc gaaaggaaa tttatttag cgttgaaag 
      tgaaccctt agctatagc agtagccac agaatggga 
      ttcccaaaa ggagaacat tattcagag taaaatgca 
      ccttacaac cttatgtaa cacagtcat tgataatcc 
      gaataattt aacaaagta cgacatggt tgactacat 
      cactaatta tctagacac cgacacgat tttatttca 
      agccaatga agaggaaga tttgctcga attgcaaga 
      aaaagagtg ggaccctat catagactg gttcaataa 
      acgctttga tgtgagtat aaaaaaatc gcaacaaat 
      ggacgcccc tccagccag tgaaaagga caaagctac 
      cctaagcaa acacctctt gtcacacaa ctttgccac 
      aatttgcgg ttttgtgta cggagtgga tacgttaaa 
      atctgttat attgacgct agcgtgtgt ggaacattt 
      cctaacacc tgaaaaagc agttctttt gtctcgact 
      ggaggagga gtatcagac ggggcactg gggcagagt 
      cgagtgggc tcatgattt gaaccaaca agacctgca 
      agccaggct agctgcagt catactttt cacctactt 
      taacaacca gtcgagtaa aattaaatc gaaatcaat 
      ccagtaata aaatcagat aagttgtaa tttttgctt 
      tatttttac tcttcagca aagcctcgt agtactgca 
      tgaattggt tttggcttt tagtcaaca tggataaac 
      tatcgattt taatcccct tttcagcga gtttttgca 
      gccttttct gcgtcgttt ccacactgt ggaccaaga 
      ttttgggca cgcggatta tgtgcaagg gttggaaac 
      cttcagcgt ataacaagt cgatgagtt ctttgagtt 
      ttttcaaac ttttgtttt ggagggctg gctgtttaa 
      ttatcatgt aatttgcca cttctacgc tgcatttga 
      ggcgttctg ataagtact cggcgaggg aactgttgc 
      ccagaatcg acttattgt cagaacgtc tttggatat 
      agctgaaaa aaaaagaca tttatttat ttatttttg 
      taattgggg gaatcacat acacttgaa acacttctt 
      gtttggctc gcaccatcc aagtttttg cagctccga 
      tgttgtatt tctttgctc gacttaaac gttcctcta 
      ttaattttt ctctgtatc ttaaaaccc acaagtcgt 
      tgcagattt cttctaatg ggatgccat aacttttta 
      gtatttcta tttttctcg tgattttat cgttcgaaa 
      gaaaacaca gaaaaggtt ttcgtgcca aatattcac 
      acgctttct gatggtcta tctgaaacc aatggttaa 
      taaaaatta cttttaaaa cacgtagaa atacattta 
      aaagtgaag aaagactcg ttatagtgc tcccttctg 
      gatccacct cattctctt ctaattttt taagactag 
      gtatgttta gtaataaaa gaattgcaa cttcattta 
      tatcatatt ttagcgcct ctataatct gtagtttct 
      gctcggttc ccgacactg ttaacaatt ctggatact 
      tcaaaatag tttctggtg ttattcctt tctgcacaa 
      gaagtgcat aattgcctt ttaaagttg ctttagaca 
      cattgttga aaaaatcat tattcaaca caatgttaa 
      acgcgtcaa aagttttaa gtcgcaaca gcttctgca 
      aagtactaa cagcccctt tcttggtca ttttatact 
      catcgtctc tcttggtgt aaaccggag ccacatttt 
      ccgattttt taacaggac ttgcagaat ataacctcg 
      catttaaat gcagtgaag gaagcaata gtcgaacat 
      cgattttca aaaactgac acaaatttc tagccattt 
      tcattaagg ggcgacaat ttttcttga agttaaggg 
      accataacc tcaaaaaat tccaaaatc atcacttct 
      tcaccttgt ttgctcgca atgtaacaa ttgttggca 
      aaaaacaac ttaattata aatttgcct gtaatttgt 
      ttcagaaat acgtataaa ctgtggtat aaataaata 
      aaaccgtgt gatgttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY