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Gene information
General information
TC013907 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid36: 409761..416979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013907
    iBeetle screen iB_02225
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    Adult morphology
    mesobasisternum posterior irregular - Number of animals showing the phenotype: 10

    intercoxal process T3 potentially shortened - Number of animals showing the phenotype: 10

    intercoxal process T3 not closed - Number of animals showing the phenotype: 10

    mesothorax coxa protruding - Number of animals showing the phenotype: 10


    motility decreased -
    Comment: all animals cannot walk or turn themselves; joint between femur and tibia cannot be mved by the animal although the joint itself is functional and movable by external manipulation

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013907 RA
      MEFAELIAT PKLDGVILH CPFHEPIDG TLCVTGHHL 
      ILSSRKEDV QELWLLHQC IDSIEKKQN SNNSNQNGG 
      SLILKCKDF RILQLDISN SDELLNVYA SIDRLSNLD 
      RPESLYPFF YRPMYNILE DGHTLFKLE SEYAKLLAS 
      EEWRISHIN KNYTVCPTY SASVIVPKS IDDDVIVAS 
      ASFRDGGRF PLLSYRHDN GAILLRSSQ PLLNNNNRR 
      SRADEKILN VILGSGKKG YIIDTRSTN YVSHCKGKG 
      GGTEPDAHY TQWKRVHKP LDKIAKCDG SLIDSLAKL 
      LDACNDTNC SFDKWMSRL ESSNWLSFV QNSLNAACL 
      VAQCLDQDG ASVLVHGTS GLDSTLLVT SVAQVILNP 
      DCRTVRGLQ ALVEREWLQ AGHPFHLRH AKSCYSNNR 
      TKSQQPTFL LFIDCIHQL YYQFPCSFE FTTQMLVLL 
      FEHSYFSQF GTFLGNNDA ERQAMKIAK KTTSLWSYL 
      NRPDVLTSL LNPLYEPNK TAIWPSVAP VSIVLWSGL 
      YLRWVINQS DNKKSLMKI QNLIDYDKN LKSKALKLR 
      KELMDLQKE YEKLQLDLG ENNPDSD
    CDS sequence
    • >TC013907 RA
      atggaattt gcggaactt atagcaaca ccaaaactg 
      gatggagtt attttacac tgtccattc cacgaaccc 
      atcgatggg acattatgt gtgacaggt caccacttg 
      attctttca tcccggaag gaagacgtt caggaattg 
      tggctttta catcaatgt attgactca attgagaaa 
      aaacaaaac agcaacaat tcgaatcaa aacgggggt 
      tccctaatc ctcaaatgt aaagacttc agaattctt 
      caattggac atttcaaac agtgacgaa ctgttaaat 
      gtttacgcc tcaattgat agattatca aacttagat 
      aggcctgaa tccctttat ccgtttttt tatcgcccc 
      atgtacaac atccttgaa gacggccac acgttattc 
      aaattagaa agcgaatac gcgaaattg ttagccagt 
      gaagagtgg agaatttca catatcaat aaaaattac 
      actgtgtgt cccacatac agtgccagt gtaattgta 
      ccaaaatca attgatgat gatgttatt gtggcctct 
      gcgagtttt agagatggg gggcggttc ccactgtta 
      agttatcgg catgataat ggggcgatt ttgttgcgt 
      agtagtcag ccgctatta aacaataat aacagaagg 
      agtagagct gacgagaaa attttaaat gtaattctg 
      ggtagcgga aaaaaagga tatattatt gatacaagg 
      agtacgaat tatgtcagt cattgtaaa ggaaaagga 
      ggcggtact gaacctgac gcacactac acacagtgg 
      aaaagggtg cataaaccg ctggataaa attgccaaa 
      tgtgatgga tctctcata gatagttta gctaaactt 
      ctagacgcc tgtaatgat acaaattgc tcattcgac 
      aagtggatg tcccgcctt gagagcagc aattggctg 
      agttttgtg cagaactcc ctaaacgct gcttgtctc 
      gtcgcccag tgtctggac caagacggg gcttcggtc 
      ctagtccat ggcacaagc ggtcttgac tcaactctc 
      ctcgtaaca tccgtagct caagtgatt ctaaaccct 
      gactgtcga acagttcga ggcctccaa gcgctagtt 
      gaacgcgag tggcttcaa gcaggccac cctttccac 
      ctacgacac gccaaaagc tgctactca aataacaga 
      acaaagagc cagcaacca acattccta ctatttatc 
      gattgtatt caccaattg tattatcaa tttccttgc 
      agttttgag ttcacaacc cagatgttg gttttatta 
      tttgaacat tcgtatttt agccaattt ggcactttt 
      cttggtaat aatgatgca gaacgacaa gcgatgaag 
      atagcaaag aaaaccaca agtttgtgg tcgtatttg 
      aatagacct gatgtctta acgtcgtta ttgaatcca 
      ttgtacgaa ccaaataaa acagcaatt tggccgagt 
      gttgcccca gttagtatc gttttgtgg tcgggtttg 
      tacctcaga tgggtcata aatcaaagt gataacaag 
      aagagttta atgaaaata caaaacctc atcgattat 
      gataaaaat ttgaagagt aaagcactg aaactaaga 
      aaggaattg atggatctt cagaaagag tacgaaaaa 
      ttgcaactt gacttggga gaaaataat cccgattca 
      gattag
    mRNA sequence
    • >TC013907 RA
      tcagacgtt tgttttata gcttgtgta ttggtgtta 
      tttaaaact ttacaaaca cataatggc gaaaaaatc 
      cacgttatt gaaactcat tatgtgata tcgtcaatt 
      cgtagttag tgtcttcct cgttacgtc gatatggaa 
      tttgcggaa cttatagca acaccaaaa ctggatgga 
      gttatttta cactgtcca ttccacgaa cccatcgat 
      gggacatta tgtgtgaca ggtcaccac ttgattctt 
      tcatcccgg aaggaagac gttcaggaa ttgtggctt 
      ttacatcaa tgtattgac tcaattgag aaaaaacaa 
      aacagcaac aattcgaat caaaacggg ggttcccta 
      atcctcaaa tgtaaagac ttcagaatt cttcaattg 
      gacatttca aacagtgac gaactgtta aatgtttac 
      gcctcaatt gatagatta tcaaactta gataggcct 
      gaatccctt tatccgttt ttttatcgc cccatgtac 
      aacatcctt gaagacggc cacacgtta ttcaaatta 
      gaaagcgaa tacgcgaaa ttgttagcc agtgaagag 
      tggagaatt tcacatatc aataaaaat tacactgtg 
      tgtcccaca tacagtgcc agtgtaatt gtaccaaaa 
      tcaattgat gatgatgtt attgtggcc tctgcgagt 
      tttagagat ggggggcgg ttcccactg ttaagttat 
      cggcatgat aatggggcg attttgttg cgtagtagt 
      cagccgcta ttaaacaat aataacaga aggagtaga 
      gctgacgag aaaatttta aatgtaatt ctgggtagc 
      ggaaaaaaa ggatatatt attgataca aggagtacg 
      aattatgtc agtcattgt aaaggaaaa ggaggcggt 
      actgaacct gacgcacac tacacacag tggaaaagg 
      gtgcataaa ccgctggat aaaattgcc aaatgtgat 
      ggatctctc atagatagt ttagctaaa cttctagac 
      gcctgtaat gatacaaat tgctcattc gacaagtgg 
      atgtcccgc cttgagagc agcaattgg ctgagtttt 
      gtgcagaac tccctaaac gctgcttgt ctcgtcgcc 
      cagtgtctg gaccaagac ggggcttcg gtcctagtc 
      catggcaca agcggtctt gactcaact ctcctcgta 
      acatccgta gctcaagtg attctaaac cctgactgt 
      cgaacagtt cgaggcctc caagcgcta gttgaacgc 
      gagtggctt caagcaggc caccctttc cacctacga 
      cacgccaaa agctgctac tcaaataac agaacaaag 
      agccagcaa ccaacattc ctactattt atcgattgt 
      attcaccaa ttgtattat caatttcct tgcagtttt 
      gagttcaca acccagatg ttggtttta ttatttgaa 
      cattcgtat tttagccaa tttggcact tttcttggt 
      aataatgat gcagaacga caagcgatg aagatagca 
      aagaaaacc acaagtttg tggtcgtat ttgaataga 
      cctgatgtc ttaacgtcg ttattgaat ccattgtac 
      gaaccaaat aaaacagca atttggccg agtgttgcc 
      ccagttagt atcgttttg tggtcgggt ttgtacctc 
      agatgggtc ataaatcaa agtgataac aagaagagt 
      ttaatgaaa atacaaaac ctcatcgat tatgataaa 
      aatttgaag agtaaagca ctgaaacta agaaaggaa 
      ttgatggat cttcagaaa gagtacgaa aaattgcaa 
      cttgacttg ggagaaaat aatcccgat tcagattag 
      ttgaatggt ggaatatta aactgtcta gtcactatt 
      aagttaaaa acaattatt gaagttggg gaagaaaca 
      tttccatga cgtgattcc aatttttaa ggaaatctt 
      tattttatt tatgctgtg ttcccagtg ttcacattt 
      attttctta caatgtaca aataaatat ttatgcact 
      tt