| General information | |||
| TC013884 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid37: 1133342..1144325 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013884 | |
| General information | ||||
| iB_02219 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC013884 RA
MPSDAKKRE QQRKKGAAK ARQAGKKAP NKNEEQAAS QNGDSISQN GSVELSAEE ALCAKLEAD ARLNAEARA CTGSLAVHP KSRDIKIDN FSITFHGCE MLQDAMLEL NCGRRYGLL GLNGSGKST ILAVLGNRE VPIPEHIDI FHLTREMPA SDKSALQCV MEVDEERVR LEKLAEELV ACEDDESQE QLMDIYERL DEISADTAE ARAANILHG LGFTKEMQS KKTKDFSGG WRMRIALAR ALYVKPHLL LLDEPTNHL DLDACVWLE EELKHYKRI LVLISHSQD FLNGVCTNI IHVNKKRLK YYTGNYDAF VRTRMELLE NQMKQYNWE QDQINHMKN YIARFGHGS AKLARQAQS KEKTLAKMV AAGLTEKVV SDKIVTFYF PSCGKIPPP VIMVQNVSF RYSDDGPWI YRNLEFGID LDTRLALVG PNGAGKSTL LKLLYGDLV PTEGMIRKN SHLRIARYH QHLHELLDL DLSPLEYMM KSFPEVKEK EEMRKIIGR YGLTGRQQV CPIRQLSDG QRCRVVFAW LAWQAPHLL LFDEPTNHL DMETIDALA EAINDFEGG MVLVSHDFR LISQVANEI WVCEKETCT KWNGTILEY KEHLKSKIL KENDKRK
- >TC013884 RA
atgccttcc gacgcgaag aaacgcgaa cagcagcgt aaaaagggg gctgcgaag gcgcgtcaa gccggaaag aaggccccc aacaagaac gaagagcaa gctgcgtcc cagaacggt gattccatt tcgcagaat ggctcagtc gagctgagt gctgaagaa gcgctttgc gcgaaactc gaagccgat gcgaggttg aatgctgag gcgagggct tgtacgggc agcttggca gtccatccc aagtcaagg gacatcaaa atcgataac ttttccatt acttttcac ggatgtgag atgctgcaa gacgctatg cttgagttg aattgcggg agacggtat ggattgttg gggctgaat ggcagtgga aagtcgact attctggca gtattaggg aacagggaa gttcccatt cctgaacat attgatatt tttcacttg actagggaa atgccagcg tcggataag tctgccttg cagtgtgtt atggaagtt gatgaggag agggtgagg ctggagaag ctggccgag gaattggtg gcgtgtgaa gatgatgaa tcgcaggag caactcatg gacatctac gagcgtctg gacgagatt tcggctgac acggccgaa gcccgtgca gccaacatc ctccacggt ttgggcttc accaaagaa atgcaaagt aagaagacc aaggacttc agcggtggc tggagaatg aggatagcg ttagctcgc gctctttac gtcaaacct catttgtta cttttggac gaaccgacc aatcatctc gatcttgac gcttgtgta tggcttgaa gaggaactc aaacattat aagagaatt ttggtttta atttcgcac tcacaggat ttcctaaac ggcgtttgc acaaacatt atccacgtt aataaaaaa cgcttgaag tactatact ggtaattat gacgctttt gttcgaact cgcatggag ttgctagag aatcaaatg aagcaatac aattgggaa caagaccag atcaaccat atgaagaat tatattgca aggtttgga cacggttcg gctaaatta gcccgtcaa gctcaatct aaggaaaaa actctagcc aaaatggtc gctgctggt ctaactgaa aaagtagtc agtgataaa atcgttacg ttttatttc cctagttgt ggaaaaatt ccgccgccc gtcatcatg gtacaaaat gttagtttt cggtatagt gatgacggt ccttggatt tatagaaat ttggaattt gggattgat ttggacacg aggctggcc cttgttgga ccgaacggt gccggaaaa agtactctg ctgaaactt ctttatggt gacttggtg cccaccgaa ggcatgatt agaaaaaac tcgcatttg aggatcgca cgataccac caacacctc cacgaactc ctagacctg gacctctcc cctctggaa tacatgatg aagtcgttc cctgaagtg aaggaaaag gaagaaatg cgtaaaatc atcggacgt tacggtcta acaggccgt cagcaagtt tgccccatt agacaattg tcagatggg caaagatgc agagtggta tttgcctgg ttggcttgg caagcccct catttgctc ctgtttgat gaacccacc aatcacttg gacatggaa acaatcgac gctctagct gaagccatc aatgatttt gaaggtgga atggtgctg gttagtcac gatttcaga ctcatcagc caggtcgct aatgaaatt tgggtttgc gagaaggag acttgcacg aaatggaac ggaacaatt ctggagtat aaggaacac ttgaaatcg aagatcttg aaagagaat gataaacgg aaataa
- >TC013884 RA
ccggctgtc tttcagtga gcggttgca cgtgaaatt tgtgttaaa ttgcaaacc tctctttaa attacttca cccgttaag tcatcatgc cttccgacg cgaagaaac gcgaacagc agcgtaaaa agggggctg cgaaggcgc gtcaagccg gaaagaagg cccccaaca agaacgaag agcaagctg cgtcccaga acggtgatt ccatttcgc agaatggct cagtcgagc tgagtgctg aagaagcgc tttgcgcga aactcgaag ccgatgcga ggttgaatg ctgaggcga gggcttgta cgggcagct tggcagtcc atcccaagt caagggaca tcaaaatcg ataactttt ccattactt ttcacggat gtgagatgc tgcaagacg ctatgcttg agttgaatt gcgggagac ggtatggat tgttggggc tgaatggca gtggaaagt cgactattc tggcagtat tagggaaca gggaagttc ccattcctg aacatattg atatttttc acttgacta gggaaatgc cagcgtcgg ataagtctg ccttgcagt gtgttatgg aagttgatg aggagaggg tgaggctgg agaagctgg ccgaggaat tggtggcgt gtgaagatg atgaatcgc aggagcaac tcatggaca tctacgagc gtctggacg agatttcgg ctgacacgg ccgaagccc gtgcagcca acatcctcc acggtttgg gcttcacca aagaaatgc aaagtaaga agaccaagg acttcagcg gtggctgga gaatgagga tagcgttag ctcgcgctc tttacgtca aacctcatt tgttacttt tggacgaac cgaccaatc atctcgatc ttgacgctt gtgtatggc ttgaagagg aactcaaac attataaga gaattttgg ttttaattt cgcactcac aggatttcc taaacggcg tttgcacaa acattatcc acgttaata aaaaacgct tgaagtact atactggta attatgacg cttttgttc gaactcgca tggagttgc tagagaatc aaatgaagc aatacaatt gggaacaag accagatca accatatga agaattata ttgcaaggt ttggacacg gttcggcta aattagccc gtcaagctc aatctaagg aaaaaactc tagccaaaa tggtcgctg ctggtctaa ctgaaaaag tagtcagtg ataaaatcg ttacgtttt atttcccta gttgtggaa aaattccgc cgcccgtca tcatggtac aaaatgtta gttttcggt atagtgatg acggtcctt ggatttata gaaatttgg aatttggga ttgatttgg acacgaggc tggcccttg ttggaccga acggtgccg gaaaaagta ctctgctga aacttcttt atggtgact tggtgccca ccgaaggca tgattagaa aaaactcgc atttgagga tcgcacgat accaccaac acctccacg aactcctag acctggacc tctcccctc tggaataca tgatgaagt cgttccctg aagtgaagg aaaaggaag aaatgcgta aaatcatcg gacgttacg gtctaacag gccgtcagc aagtttgcc ccattagac aattgtcag atgggcaaa gatgcagag tggtatttg cctggttgg cttggcaag cccctcatt tgctcctgt ttgatgaac ccaccaatc acttggaca tggaaacaa tcgacgctc tagctgaag ccatcaatg attttgaag gtggaatgg tgctggtta gtcacgatt tcagactca tcagccagg tcgctaatg aaatttggg tttgcgaga aggagactt gcacgaaat ggaacggaa caattctgg agtataagg aacacttga aatcgaaga tcttgaaag agaatgata aacggaaat aacttgtcc agtttctag gtttttcat ttttcgata atagtaaat tatttttat ggaaacatt gtatgatct tgtacagtt tttagatga aattaattt attatttag ttaatacca ggttttgac ctttttgca ttcgcttgt actaaccgc aatttttta tttatacag tcattgggt acacctttg ccatttttg gcgagttcc aaggcaatt aaaccgttt aacgttcaa aaggtattt tattcgacc aagaaattt aattttcta ttgcacgac tattttaag aacaccaat ttttaagaa tgtagacgt gtttagggc gggataggt cagttctgg attttaccg tcgtaaaca ttttattta taaactata caaataagt aataaagaa taccaagtt gtccgtttt atcgcagca tccttgata agtgaaggt agtttcccc tttttttgt ctcacatca gtgttacta tagttataa ttcatttcg taaccacgg aaaaaagaa gaaattaac agatggcgc cacattgcg gggaatatc tatggggaa ccttcatta agagagagt taatccaaa attctgatt tggggaaca cagtcttaa ttctgtata ataatacca tgtgatcat ttgaattta taatacttt ttggaatca caattaatc aagatgaaa tgataaata ataaaattt cgaaccata tttagttac attcatttt ttccttcag tttgcacta atttcttaa tcataaagg aaaaaaaag cctactcta attaataaa tagtacggt ggaaattaa atgttcata atagagcta aagatatga cccgagata agaaattaa aaaaaaaat tgttgccat tttaattga taaaaatat aaagatatt aattacatt taataaaat gcatccaat attattaaa ttcatgtga ctagaagaa aatattgta tgttggcca catggatga ccaaagata aaaaaatta ataaaattt tgtattata aagatattt ttgtggatt ggtgatccg gaaacaatt ttatttgca ttgtatagt tccagaact tgtaaatcc agtagactt tacacataa atggctatt agaaaggtt aagtgtatt cttaaagct cacaagaca aaaaaatga ataacgatt atacaggtg ctttaaaat tggcgaatt tagagttca cagcattgt agaaaatca cttagtagt ttaaatata taaaaaaaa ttcggggct tccccagaa agatacact ggtttgaag atcactctt tgaattgcg ttttcttca aagatttta taacttcca cattcattg gcctggctc tgatttggg ttgcgttta gccagccct tttgtccac agcagatgt gtagtgtaa ttattaaaa tggtaagta ctttaatag agaatagtg gtgttatta tattattag ttattacag ctaaaaatt attagtctt ttttgttct ttacagtat aatttcatt atcagggtt ccttgccca caagaatta caagtaccc taacatgaa acaaaaaaa cgccctgat tattcaaat caacttgtg tcaatcata gaaatagca atgtgttgg acgtccgag aattaatct cgagttccc gatcatcat caaggaatg atctttggt attgaaaaa ttactcaac cacataatc gaagaacaa aaaaagatg aaatgaaga tgaatatta gtgagtatg aagtaccaa aatctgttt caagtatct gttgatctg cactactgt tcgtgcaaa aagccaact ttttgttta actgaaaac atcaaaaaa ttaaatttt aatggttat tgtgaactt gcaagagta atatttggg ttaagatga agtgaatga gttgtggaa tgcgataaa tttattctt ggtcttatt attgttaca tattctttg ggtttatag aaataaatg ttattaatt agc