Status
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Gene information
General information
TC013884 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2168550..2179533
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013884
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02219

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013884 RA
      MPSDAKKRE QQRKKGAAK ARQAGKKAP NKNEEQAAS 
      QNGDSISQN GSVELSAEE ALCAKLEAD ARLNAEARA 
      CTGSLAVHP KSRDIKIDN FSITFHGCE MLQDAMLEL 
      NCGRRYGLL GLNGSGKST ILAVLGNRE VPIPEHIDI 
      FHLTREMPA SDKSALQCV MEVDEERVR LEKLAEELV 
      ACEDDESQE QLMDIYERL DEISADTAE ARAANILHG 
      LGFTKEMQS KKTKDFSGG WRMRIALAR ALYVKPHLL 
      LLDEPTNHL DLDACVWLE EELKHYKRI LVLISHSQD 
      FLNGVCTNI IHVNKKRLK YYTGNYDAF VRTRMELLE 
      NQMKQYNWE QDQINHMKN YIARFGHGS AKLARQAQS 
      KEKTLAKMV AAGLTEKVV SDKIVTFYF PSCGKIPPP 
      VIMVQNVSF RYSDDGPWI YRNLEFGID LDTRLALVG 
      PNGAGKSTL LKLLYGDLV PTEGMIRKN SHLRIARYH 
      QHLHELLDL DLSPLEYMM KSFPEVKEK EEMRKIIGR 
      YGLTGRQQV CPIRQLSDG QRCRVVFAW LAWQAPHLL 
      LFDEPTNHL DMETIDALA EAINDFEGG MVLVSHDFR 
      LISQVANEI WVCEKETCT KWNGTILEY KEHLKSKIL 
      KENDKRK
    CDS sequence
    • >TC013884 RA
      atgccttcc gacgcgaag aaacgcgaa cagcagcgt 
      aaaaagggg gctgcgaag gcgcgtcaa gccggaaag 
      aaggccccc aacaagaac gaagagcaa gctgcgtcc 
      cagaacggt gattccatt tcgcagaat ggctcagtc 
      gagctgagt gctgaagaa gcgctttgc gcgaaactc 
      gaagccgat gcgaggttg aatgctgag gcgagggct 
      tgtacgggc agcttggca gtccatccc aagtcaagg 
      gacatcaaa atcgataac ttttccatt acttttcac 
      ggatgtgag atgctgcaa gacgctatg cttgagttg 
      aattgcggg agacggtat ggattgttg gggctgaat 
      ggcagtgga aagtcgact attctggca gtattaggg 
      aacagggaa gttcccatt cctgaacat attgatatt 
      tttcacttg actagggaa atgccagcg tcggataag 
      tctgccttg cagtgtgtt atggaagtt gatgaggag 
      agggtgagg ctggagaag ctggccgag gaattggtg 
      gcgtgtgaa gatgatgaa tcgcaggag caactcatg 
      gacatctac gagcgtctg gacgagatt tcggctgac 
      acggccgaa gcccgtgca gccaacatc ctccacggt 
      ttgggcttc accaaagaa atgcaaagt aagaagacc 
      aaggacttc agcggtggc tggagaatg aggatagcg 
      ttagctcgc gctctttac gtcaaacct catttgtta 
      cttttggac gaaccgacc aatcatctc gatcttgac 
      gcttgtgta tggcttgaa gaggaactc aaacattat 
      aagagaatt ttggtttta atttcgcac tcacaggat 
      ttcctaaac ggcgtttgc acaaacatt atccacgtt 
      aataaaaaa cgcttgaag tactatact ggtaattat 
      gacgctttt gttcgaact cgcatggag ttgctagag 
      aatcaaatg aagcaatac aattgggaa caagaccag 
      atcaaccat atgaagaat tatattgca aggtttgga 
      cacggttcg gctaaatta gcccgtcaa gctcaatct 
      aaggaaaaa actctagcc aaaatggtc gctgctggt 
      ctaactgaa aaagtagtc agtgataaa atcgttacg 
      ttttatttc cctagttgt ggaaaaatt ccgccgccc 
      gtcatcatg gtacaaaat gttagtttt cggtatagt 
      gatgacggt ccttggatt tatagaaat ttggaattt 
      gggattgat ttggacacg aggctggcc cttgttgga 
      ccgaacggt gccggaaaa agtactctg ctgaaactt 
      ctttatggt gacttggtg cccaccgaa ggcatgatt 
      agaaaaaac tcgcatttg aggatcgca cgataccac 
      caacacctc cacgaactc ctagacctg gacctctcc 
      cctctggaa tacatgatg aagtcgttc cctgaagtg 
      aaggaaaag gaagaaatg cgtaaaatc atcggacgt 
      tacggtcta acaggccgt cagcaagtt tgccccatt 
      agacaattg tcagatggg caaagatgc agagtggta 
      tttgcctgg ttggcttgg caagcccct catttgctc 
      ctgtttgat gaacccacc aatcacttg gacatggaa 
      acaatcgac gctctagct gaagccatc aatgatttt 
      gaaggtgga atggtgctg gttagtcac gatttcaga 
      ctcatcagc caggtcgct aatgaaatt tgggtttgc 
      gagaaggag acttgcacg aaatggaac ggaacaatt 
      ctggagtat aaggaacac ttgaaatcg aagatcttg 
      aaagagaat gataaacgg aaataa
    mRNA sequence
    • >TC013884 RA
      ccggctgtc tttcagtga gcggttgca cgtgaaatt 
      tgtgttaaa ttgcaaacc tctctttaa attacttca 
      cccgttaag tcatcatgc cttccgacg cgaagaaac 
      gcgaacagc agcgtaaaa agggggctg cgaaggcgc 
      gtcaagccg gaaagaagg cccccaaca agaacgaag 
      agcaagctg cgtcccaga acggtgatt ccatttcgc 
      agaatggct cagtcgagc tgagtgctg aagaagcgc 
      tttgcgcga aactcgaag ccgatgcga ggttgaatg 
      ctgaggcga gggcttgta cgggcagct tggcagtcc 
      atcccaagt caagggaca tcaaaatcg ataactttt 
      ccattactt ttcacggat gtgagatgc tgcaagacg 
      ctatgcttg agttgaatt gcgggagac ggtatggat 
      tgttggggc tgaatggca gtggaaagt cgactattc 
      tggcagtat tagggaaca gggaagttc ccattcctg 
      aacatattg atatttttc acttgacta gggaaatgc 
      cagcgtcgg ataagtctg ccttgcagt gtgttatgg 
      aagttgatg aggagaggg tgaggctgg agaagctgg 
      ccgaggaat tggtggcgt gtgaagatg atgaatcgc 
      aggagcaac tcatggaca tctacgagc gtctggacg 
      agatttcgg ctgacacgg ccgaagccc gtgcagcca 
      acatcctcc acggtttgg gcttcacca aagaaatgc 
      aaagtaaga agaccaagg acttcagcg gtggctgga 
      gaatgagga tagcgttag ctcgcgctc tttacgtca 
      aacctcatt tgttacttt tggacgaac cgaccaatc 
      atctcgatc ttgacgctt gtgtatggc ttgaagagg 
      aactcaaac attataaga gaattttgg ttttaattt 
      cgcactcac aggatttcc taaacggcg tttgcacaa 
      acattatcc acgttaata aaaaacgct tgaagtact 
      atactggta attatgacg cttttgttc gaactcgca 
      tggagttgc tagagaatc aaatgaagc aatacaatt 
      gggaacaag accagatca accatatga agaattata 
      ttgcaaggt ttggacacg gttcggcta aattagccc 
      gtcaagctc aatctaagg aaaaaactc tagccaaaa 
      tggtcgctg ctggtctaa ctgaaaaag tagtcagtg 
      ataaaatcg ttacgtttt atttcccta gttgtggaa 
      aaattccgc cgcccgtca tcatggtac aaaatgtta 
      gttttcggt atagtgatg acggtcctt ggatttata 
      gaaatttgg aatttggga ttgatttgg acacgaggc 
      tggcccttg ttggaccga acggtgccg gaaaaagta 
      ctctgctga aacttcttt atggtgact tggtgccca 
      ccgaaggca tgattagaa aaaactcgc atttgagga 
      tcgcacgat accaccaac acctccacg aactcctag 
      acctggacc tctcccctc tggaataca tgatgaagt 
      cgttccctg aagtgaagg aaaaggaag aaatgcgta 
      aaatcatcg gacgttacg gtctaacag gccgtcagc 
      aagtttgcc ccattagac aattgtcag atgggcaaa 
      gatgcagag tggtatttg cctggttgg cttggcaag 
      cccctcatt tgctcctgt ttgatgaac ccaccaatc 
      acttggaca tggaaacaa tcgacgctc tagctgaag 
      ccatcaatg attttgaag gtggaatgg tgctggtta 
      gtcacgatt tcagactca tcagccagg tcgctaatg 
      aaatttggg tttgcgaga aggagactt gcacgaaat 
      ggaacggaa caattctgg agtataagg aacacttga 
      aatcgaaga tcttgaaag agaatgata aacggaaat 
      aacttgtcc agtttctag gtttttcat ttttcgata 
      atagtaaat tatttttat ggaaacatt gtatgatct 
      tgtacagtt tttagatga aattaattt attatttag 
      ttaatacca ggttttgac ctttttgca ttcgcttgt 
      actaaccgc aatttttta tttatacag tcattgggt 
      acacctttg ccatttttg gcgagttcc aaggcaatt 
      aaaccgttt aacgttcaa aaggtattt tattcgacc 
      aagaaattt aattttcta ttgcacgac tattttaag 
      aacaccaat ttttaagaa tgtagacgt gtttagggc 
      gggataggt cagttctgg attttaccg tcgtaaaca 
      ttttattta taaactata caaataagt aataaagaa 
      taccaagtt gtccgtttt atcgcagca tccttgata 
      agtgaaggt agtttcccc tttttttgt ctcacatca 
      gtgttacta tagttataa ttcatttcg taaccacgg 
      aaaaaagaa gaaattaac agatggcgc cacattgcg 
      gggaatatc tatggggaa ccttcatta agagagagt 
      taatccaaa attctgatt tggggaaca cagtcttaa 
      ttctgtata ataatacca tgtgatcat ttgaattta 
      taatacttt ttggaatca caattaatc aagatgaaa 
      tgataaata ataaaattt cgaaccata tttagttac 
      attcatttt ttccttcag tttgcacta atttcttaa 
      tcataaagg aaaaaaaag cctactcta attaataaa 
      tagtacggt ggaaattaa atgttcata atagagcta 
      aagatatga cccgagata agaaattaa aaaaaaaat 
      tgttgccat tttaattga taaaaatat aaagatatt 
      aattacatt taataaaat gcatccaat attattaaa 
      ttcatgtga ctagaagaa aatattgta tgttggcca 
      catggatga ccaaagata aaaaaatta ataaaattt 
      tgtattata aagatattt ttgtggatt ggtgatccg 
      gaaacaatt ttatttgca ttgtatagt tccagaact 
      tgtaaatcc agtagactt tacacataa atggctatt 
      agaaaggtt aagtgtatt cttaaagct cacaagaca 
      aaaaaatga ataacgatt atacaggtg ctttaaaat 
      tggcgaatt tagagttca cagcattgt agaaaatca 
      cttagtagt ttaaatata taaaaaaaa ttcggggct 
      tccccagaa agatacact ggtttgaag atcactctt 
      tgaattgcg ttttcttca aagatttta taacttcca 
      cattcattg gcctggctc tgatttggg ttgcgttta 
      gccagccct tttgtccac agcagatgt gtagtgtaa 
      ttattaaaa tggtaagta ctttaatag agaatagtg 
      gtgttatta tattattag ttattacag ctaaaaatt 
      attagtctt ttttgttct ttacagtat aatttcatt 
      atcagggtt ccttgccca caagaatta caagtaccc 
      taacatgaa acaaaaaaa cgccctgat tattcaaat 
      caacttgtg tcaatcata gaaatagca atgtgttgg 
      acgtccgag aattaatct cgagttccc gatcatcat 
      caaggaatg atctttggt attgaaaaa ttactcaac 
      cacataatc gaagaacaa aaaaagatg aaatgaaga 
      tgaatatta gtgagtatg aagtaccaa aatctgttt 
      caagtatct gttgatctg cactactgt tcgtgcaaa 
      aagccaact ttttgttta actgaaaac atcaaaaaa 
      ttaaatttt aatggttat tgtgaactt gcaagagta 
      atatttggg ttaagatga agtgaatga gttgtggaa 
      tgcgataaa tttattctt ggtcttatt attgttaca 
      tattctttg ggtttatag aaataaatg ttattaatt 
      agc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY