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Gene information
General information
TC013884 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2168550..2179533
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013884
    iBeetle screen iB_02219

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013884 RA
      MPSDAKKRE QQRKKGAAK ARQAGKKAP NKNEEQAAS 
QNGDSISQN GSVELSAEE ALCAKLEAD ARLNAEARA 
CTGSLAVHP KSRDIKIDN FSITFHGCE MLQDAMLEL 
NCGRRYGLL GLNGSGKST ILAVLGNRE VPIPEHIDI 
FHLTREMPA SDKSALQCV MEVDEERVR LEKLAEELV 
ACEDDESQE QLMDIYERL DEISADTAE ARAANILHG 
LGFTKEMQS KKTKDFSGG WRMRIALAR ALYVKPHLL 
LLDEPTNHL DLDACVWLE EELKHYKRI LVLISHSQD 
FLNGVCTNI IHVNKKRLK YYTGNYDAF VRTRMELLE 
NQMKQYNWE QDQINHMKN YIARFGHGS AKLARQAQS 
KEKTLAKMV AAGLTEKVV SDKIVTFYF PSCGKIPPP 
VIMVQNVSF RYSDDGPWI YRNLEFGID LDTRLALVG 
PNGAGKSTL LKLLYGDLV PTEGMIRKN SHLRIARYH 
QHLHELLDL DLSPLEYMM KSFPEVKEK EEMRKIIGR 
YGLTGRQQV CPIRQLSDG QRCRVVFAW LAWQAPHLL 
LFDEPTNHL DMETIDALA EAINDFEGG MVLVSHDFR 
LISQVANEI WVCEKETCT KWNGTILEY KEHLKSKIL 
KENDKRK
    CDS sequence
    • >TC013884 RA
      atgccttcc gacgcgaag aaacgcgaa cagcagcgt 
aaaaagggg gctgcgaag gcgcgtcaa gccggaaag 
aaggccccc aacaagaac gaagagcaa gctgcgtcc 
cagaacggt gattccatt tcgcagaat ggctcagtc 
gagctgagt gctgaagaa gcgctttgc gcgaaactc 
gaagccgat gcgaggttg aatgctgag gcgagggct 
tgtacgggc agcttggca gtccatccc aagtcaagg 
gacatcaaa atcgataac ttttccatt acttttcac 
ggatgtgag atgctgcaa gacgctatg cttgagttg 
aattgcggg agacggtat ggattgttg gggctgaat 
ggcagtgga aagtcgact attctggca gtattaggg 
aacagggaa gttcccatt cctgaacat attgatatt 
tttcacttg actagggaa atgccagcg tcggataag 
tctgccttg cagtgtgtt atggaagtt gatgaggag 
agggtgagg ctggagaag ctggccgag gaattggtg 
gcgtgtgaa gatgatgaa tcgcaggag caactcatg 
gacatctac gagcgtctg gacgagatt tcggctgac 
acggccgaa gcccgtgca gccaacatc ctccacggt 
ttgggcttc accaaagaa atgcaaagt aagaagacc 
aaggacttc agcggtggc tggagaatg aggatagcg 
ttagctcgc gctctttac gtcaaacct catttgtta 
cttttggac gaaccgacc aatcatctc gatcttgac 
gcttgtgta tggcttgaa gaggaactc aaacattat 
aagagaatt ttggtttta atttcgcac tcacaggat 
ttcctaaac ggcgtttgc acaaacatt atccacgtt 
aataaaaaa cgcttgaag tactatact ggtaattat 
gacgctttt gttcgaact cgcatggag ttgctagag 
aatcaaatg aagcaatac aattgggaa caagaccag 
atcaaccat atgaagaat tatattgca aggtttgga 
cacggttcg gctaaatta gcccgtcaa gctcaatct 
aaggaaaaa actctagcc aaaatggtc gctgctggt 
ctaactgaa aaagtagtc agtgataaa atcgttacg 
ttttatttc cctagttgt ggaaaaatt ccgccgccc 
gtcatcatg gtacaaaat gttagtttt cggtatagt 
gatgacggt ccttggatt tatagaaat ttggaattt 
gggattgat ttggacacg aggctggcc cttgttgga 
ccgaacggt gccggaaaa agtactctg ctgaaactt 
ctttatggt gacttggtg cccaccgaa ggcatgatt 
agaaaaaac tcgcatttg aggatcgca cgataccac 
caacacctc cacgaactc ctagacctg gacctctcc 
cctctggaa tacatgatg aagtcgttc cctgaagtg 
aaggaaaag gaagaaatg cgtaaaatc atcggacgt 
tacggtcta acaggccgt cagcaagtt tgccccatt 
agacaattg tcagatggg caaagatgc agagtggta 
tttgcctgg ttggcttgg caagcccct catttgctc 
ctgtttgat gaacccacc aatcacttg gacatggaa 
acaatcgac gctctagct gaagccatc aatgatttt 
gaaggtgga atggtgctg gttagtcac gatttcaga 
ctcatcagc caggtcgct aatgaaatt tgggtttgc 
gagaaggag acttgcacg aaatggaac ggaacaatt 
ctggagtat aaggaacac ttgaaatcg aagatcttg 
aaagagaat gataaacgg aaataa
    mRNA sequence
    • >TC013884 RA
      ccggctgtc tttcagtga gcggttgca cgtgaaatt 
tgtgttaaa ttgcaaacc tctctttaa attacttca 
cccgttaag tcatcatgc cttccgacg cgaagaaac 
gcgaacagc agcgtaaaa agggggctg cgaaggcgc 
gtcaagccg gaaagaagg cccccaaca agaacgaag 
agcaagctg cgtcccaga acggtgatt ccatttcgc 
agaatggct cagtcgagc tgagtgctg aagaagcgc 
tttgcgcga aactcgaag ccgatgcga ggttgaatg 
ctgaggcga gggcttgta cgggcagct tggcagtcc 
atcccaagt caagggaca tcaaaatcg ataactttt 
ccattactt ttcacggat gtgagatgc tgcaagacg 
ctatgcttg agttgaatt gcgggagac ggtatggat 
tgttggggc tgaatggca gtggaaagt cgactattc 
tggcagtat tagggaaca gggaagttc ccattcctg 
aacatattg atatttttc acttgacta gggaaatgc 
cagcgtcgg ataagtctg ccttgcagt gtgttatgg 
aagttgatg aggagaggg tgaggctgg agaagctgg 
ccgaggaat tggtggcgt gtgaagatg atgaatcgc 
aggagcaac tcatggaca tctacgagc gtctggacg 
agatttcgg ctgacacgg ccgaagccc gtgcagcca 
acatcctcc acggtttgg gcttcacca aagaaatgc 
aaagtaaga agaccaagg acttcagcg gtggctgga 
gaatgagga tagcgttag ctcgcgctc tttacgtca 
aacctcatt tgttacttt tggacgaac cgaccaatc 
atctcgatc ttgacgctt gtgtatggc ttgaagagg 
aactcaaac attataaga gaattttgg ttttaattt 
cgcactcac aggatttcc taaacggcg tttgcacaa 
acattatcc acgttaata aaaaacgct tgaagtact 
atactggta attatgacg cttttgttc gaactcgca 
tggagttgc tagagaatc aaatgaagc aatacaatt 
gggaacaag accagatca accatatga agaattata 
ttgcaaggt ttggacacg gttcggcta aattagccc 
gtcaagctc aatctaagg aaaaaactc tagccaaaa 
tggtcgctg ctggtctaa ctgaaaaag tagtcagtg 
ataaaatcg ttacgtttt atttcccta gttgtggaa 
aaattccgc cgcccgtca tcatggtac aaaatgtta 
gttttcggt atagtgatg acggtcctt ggatttata 
gaaatttgg aatttggga ttgatttgg acacgaggc 
tggcccttg ttggaccga acggtgccg gaaaaagta 
ctctgctga aacttcttt atggtgact tggtgccca 
ccgaaggca tgattagaa aaaactcgc atttgagga 
tcgcacgat accaccaac acctccacg aactcctag 
acctggacc tctcccctc tggaataca tgatgaagt 
cgttccctg aagtgaagg aaaaggaag aaatgcgta 
aaatcatcg gacgttacg gtctaacag gccgtcagc 
aagtttgcc ccattagac aattgtcag atgggcaaa 
gatgcagag tggtatttg cctggttgg cttggcaag 
cccctcatt tgctcctgt ttgatgaac ccaccaatc 
acttggaca tggaaacaa tcgacgctc tagctgaag 
ccatcaatg attttgaag gtggaatgg tgctggtta 
gtcacgatt tcagactca tcagccagg tcgctaatg 
aaatttggg tttgcgaga aggagactt gcacgaaat 
ggaacggaa caattctgg agtataagg aacacttga 
aatcgaaga tcttgaaag agaatgata aacggaaat 
aacttgtcc agtttctag gtttttcat ttttcgata 
atagtaaat tatttttat ggaaacatt gtatgatct 
tgtacagtt tttagatga aattaattt attatttag 
ttaatacca ggttttgac ctttttgca ttcgcttgt 
actaaccgc aatttttta tttatacag tcattgggt 
acacctttg ccatttttg gcgagttcc aaggcaatt 
aaaccgttt aacgttcaa aaggtattt tattcgacc 
aagaaattt aattttcta ttgcacgac tattttaag 
aacaccaat ttttaagaa tgtagacgt gtttagggc 
gggataggt cagttctgg attttaccg tcgtaaaca 
ttttattta taaactata caaataagt aataaagaa 
taccaagtt gtccgtttt atcgcagca tccttgata 
agtgaaggt agtttcccc tttttttgt ctcacatca 
gtgttacta tagttataa ttcatttcg taaccacgg 
aaaaaagaa gaaattaac agatggcgc cacattgcg 
gggaatatc tatggggaa ccttcatta agagagagt 
taatccaaa attctgatt tggggaaca cagtcttaa 
ttctgtata ataatacca tgtgatcat ttgaattta 
taatacttt ttggaatca caattaatc aagatgaaa 
tgataaata ataaaattt cgaaccata tttagttac 
attcatttt ttccttcag tttgcacta atttcttaa 
tcataaagg aaaaaaaag cctactcta attaataaa 
tagtacggt ggaaattaa atgttcata atagagcta 
aagatatga cccgagata agaaattaa aaaaaaaat 
tgttgccat tttaattga taaaaatat aaagatatt 
aattacatt taataaaat gcatccaat attattaaa 
ttcatgtga ctagaagaa aatattgta tgttggcca 
catggatga ccaaagata aaaaaatta ataaaattt 
tgtattata aagatattt ttgtggatt ggtgatccg 
gaaacaatt ttatttgca ttgtatagt tccagaact 
tgtaaatcc agtagactt tacacataa atggctatt 
agaaaggtt aagtgtatt cttaaagct cacaagaca 
aaaaaatga ataacgatt atacaggtg ctttaaaat 
tggcgaatt tagagttca cagcattgt agaaaatca 
cttagtagt ttaaatata taaaaaaaa ttcggggct 
tccccagaa agatacact ggtttgaag atcactctt 
tgaattgcg ttttcttca aagatttta taacttcca 
cattcattg gcctggctc tgatttggg ttgcgttta 
gccagccct tttgtccac agcagatgt gtagtgtaa 
ttattaaaa tggtaagta ctttaatag agaatagtg 
gtgttatta tattattag ttattacag ctaaaaatt 
attagtctt ttttgttct ttacagtat aatttcatt 
atcagggtt ccttgccca caagaatta caagtaccc 
taacatgaa acaaaaaaa cgccctgat tattcaaat 
caacttgtg tcaatcata gaaatagca atgtgttgg 
acgtccgag aattaatct cgagttccc gatcatcat 
caaggaatg atctttggt attgaaaaa ttactcaac 
cacataatc gaagaacaa aaaaagatg aaatgaaga 
tgaatatta gtgagtatg aagtaccaa aatctgttt 
caagtatct gttgatctg cactactgt tcgtgcaaa 
aagccaact ttttgttta actgaaaac atcaaaaaa 
ttaaatttt aatggttat tgtgaactt gcaagagta 
atatttggg ttaagatga agtgaatga gttgtggaa 
tgcgataaa tttattctt ggtcttatt attgttaca 
tattctttg ggtttatag aaataaatg ttattaatt 
agc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY