Status
Please wait ...
Gene information
General information
TC013882 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2151090..2158683
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013882
    iBeetle screen iB_02218

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber segmentation/division irregular - Number of animals showing the phenotype: 4

    Comment: Contact between mid stage egg chambers is potentially to large. Maybe due to missing stalk cells?
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    about 20
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 25

    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    Pupal morphology
    labrum size decreased - Number of animals showing the phenotype: 9

    head orientation irregular - Number of animals showing the phenotype: 8

    elytra blistered - Number of animals showing the phenotype: 2

    elytra necrotic - Number of animals showing the phenotype: 10

    wings shortened - Number of animals showing the phenotype: 2
    wings orientation irregular - Number of animals showing the phenotype: 2
    legs tarsus shape irregular - Number of animals showing the phenotype: 2

    wings potentially blistered - Number of animals showing the phenotype: 8

    Comment: animals are dead for too long to make definitive statements and wings are clotted with flour
    wings potentially shortened - Number of animals showing the phenotype: 8
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013882 RA
      MSNLRGDLD PSLDQSFEQ KMQYMSLND SNPRYGRIM 
      GKAEPAVPP YHQKQKSMG AIESFVAEG PTQQKSNDY 
      TLYERNNII ASSKYATPK QVETLVQKQ VEENDIYVQ 
      CAKPQVPPN ASPTHSLSG SSQHSGSPR TSMANQTPV 
      YENIDYYST RNYQPPYYH SMESNYRKA QPQVPSINR 
      NITRDGEAL PVYENLQSL GQKSSGATP GPQVPSNQA 
      PPPYPTQSY QQTYPQMHK TVLKAPYYP SYNKNLTKQ 
      QLDEINSSD YVCMTGNIS HTLSTNVPF QTSTAKNYN 
      REPGTGIAT TPSVLPKSP IVKEKVEVR PPPVPSPTP 
      SACSTLSAG KLKMSGKTL LPYNVTPPR PRGPTEAER 
      KIEEMTRQI EEEMEKHEE EGEYFGICH TCGEKVTGA 
      GQACQAMGN LYHTNCFIC CSCGRALRG KAFYNVHGR 
      VYCEEDYLY SGFQQTAEK CAICGHLIM EMILQAMGK 
      SYHPGCFRC CICNECLDG VPFTVDVDN KIYCVNDYH 
      RMFAPKCAS CGKGITPVE GTEETVRVV SMDKDFHVD 
      CYICEECGM QLTDEPDKR CYPLEGRLM CRSCHIERL 
      QHTPRQMHP VAATYQYTG
    CDS sequence
    • >TC013882 RA
      atgtcgaac cttcgcggt gacttggat ccttctttg 
      gatcagtcg ttcgagcaa aaaatgcag tacatgtcc 
      ctcaacgac tcgaatcca cggtacggc cgcatcatg 
      gggaaggca gagccggcg gtgccccct taccaccag 
      aaacaaaaa tcgatgggg gccatcgaa agtttcgta 
      gcagaagga ccgacccag cagaaatca aacgattac 
      actttgtat gaaagaaac aacatcatt gccagttct 
      aaatacgcc actcctaaa caagtcgag accctagta 
      cagaagcaa gtcgaagag aatgatatt tacgtccag 
      tgcgctaag ccccaagta cccccaaat gcgagcccc 
      actcactct ttaagcggc tcatcgcaa cactccggc 
      agcccccga accagcatg gccaaccaa accccagtt 
      tacgaaaac atcgactac tactccact cggaactac 
      caaccccct tactaccac tccatggaa agtaactac 
      cggaaagcc cagccccaa gtcccctca atcaaccgc 
      aacatcacg cgtgatggt gaggctttg cccgtttac 
      gaaaacttg cagtctttg gggcagaaa tccagcggg 
      gctacgccg gggccccaa gtgccttca aatcaagca 
      ccgcctcca taccccacc caatcgtac caacagacg 
      tacccccaa atgcacaaa acggttctc aaagccccg 
      tactacccc agttataat aaaaatttg actaaacaa 
      cagctggat gaaatcaat agcagtgat tatgtctgt 
      atgactggg aatatctcg cacactttg agtacgaac 
      gtgccgttt caaacgtcc actgccaaa aactacaat 
      agagagcct ggtactggc attgccact acgccgtca 
      gttttgccc aaaagtccg attgtgaag gagaaagtt 
      gaggtgaga ccgccaccg gttccgagc cccacgccc 
      agcgcttgt agtactttg agtgctggg aaattgaaa 
      atgagtgga aagactttg ttgccctat aatgttaca 
      ccgccgagg ccaaggggg cccacggag gcagaaagg 
      aaaattgag gagatgact aggcagatt gaagaggaa 
      atggagaag catgaggag gaaggggaa tattttggt 
      atttgccac acatgcggc gaaaaagtg accggcgcc 
      ggtcaagca tgccaagca atgggcaac ttgtaccat 
      acgaactgt tttatatgt tgttcgtgt ggtcgtgcc 
      ttacgcggc aaagctttc tataatgtt catggaaga 
      gtctactgc gaagaagac tacctgtat tcaggattt 
      cagcaaact gcggaaaaa tgtgccatt tgtggccat 
      ttaattatg gagatgatt ctacaagcc atgggcaag 
      tcgtatcat ccagggtgt ttccgttgt tgcatttgt 
      aacgaatgc ttagacggt gtgcctttc actgtggac 
      gtcgacaat aaaatttat tgcgtcaat gattaccac 
      aggatgttc gccccgaaa tgtgcttcg tgtggcaaa 
      ggtataaca ccagttgag ggtacagaa gaaaccgtc 
      cgagtggtc tccatggat aaggacttc cacgttgac 
      tgttacata tgcgaagaa tgtggaatg cagttaact 
      gacgaacct gacaagcgt tgttatcca cttgagggg 
      cgtttgatg tgtcgaagt tgtcacatt gagcgcctc 
      caacatacc cccaggcag atgcatcct gttgctgct 
      acatatcag tatacgggc taa
    mRNA sequence
    • >TC013882 RA
      tcggtcgtt ttattacct catctccgg ttaattatg 
      tatttattt atgagtaat tattgaaat atgtcgatt 
      gagttactt aatttaagt gaaaactgc cgttctgtg 
      attaaatca atttggtga taatgtcga accttcgcg 
      gtgacttgg atccttctt tggatcagt cgttcgagc 
      aaaaaatgc agtacatgt ccctcaacg actcgaatc 
      cacggtacg gccgcatca tggggaagg cagagccgg 
      cggtgcccc cttaccacc agaaacaaa aatcgatgg 
      gggccatcg aaagtttcg tagcagaag gaccgaccc 
      agcagaaat caaacgatt acactttgt atgaaagaa 
      acaacatca ttgccagtt ctaaatacg ccactccta 
      aacaagtcg agaccctag tacagaagc aagtcgaag 
      agaatgata tttacgtcc agtgcgcta agccccaag 
      tacccccaa atgcgagcc ccactcact ctttaagcg 
      gctcatcgc aacactccg gcagccccc gaaccagca 
      tggccaacc aaaccccag tttacgaaa acatcgact 
      actactcca ctcggaact accaacccc cttactacc 
      actccatgg aaagtaact accggaaag cccagcccc 
      aagtcccct caatcaacc gcaacatca cgcgtgatg 
      gtgaggctt tgcccgttt acgaaaact tgcagtctt 
      tggggcaga aatccagcg gggctacgc cggggcccc 
      aagtgcctt caaatcaag caccgcctc catacccca 
      cccaatcgt accaacaga cgtaccccc aaatgcaca 
      aaacggttc tcaaagccc cgtactacc ccagttata 
      ataaaaatt tgactaaac aacagctgg atgaaatca 
      atagcagtg attatgtct gtatgactg ggaatatct 
      cgcacactt tgagtacga acgtgccgt ttcaaacgt 
      ccactgcca aaaactaca atagagagc ctggtactg 
      gcattgcca ctacgccgt cagttttgc ccaaaagtc 
      cgattgtga aggagaaag ttgaggtga gaccgccac 
      cggttccga gccccacgc ccagcgctt gtagtactt 
      tgagtgctg ggaaattga aaatgagtg gaaagactt 
      tgttgccct ataatgtta caccgccga ggccaaggg 
      ggcccacgg aggcagaaa ggaaaattg aggagatga 
      ctaggcaga ttgaagagg aaatggaga agcatgagg 
      aggaagggg aatattttg gtatttgcc acacatgcg 
      gcgaaaaag tgaccggcg ccggtcaag catgccaag 
      caatgggca acttgtacc atacgaact gttttatat 
      gttgttcgt gtggtcgtg ccttacgcg gcaaagctt 
      tctataatg ttcatggaa gagtctact gcgaagaag 
      actacctgt attcaggat ttcagcaaa ctgcggaaa 
      aatgtgcca tttgtggcc atttaatta tggagatga 
      ttctacaag ccatgggca agtcgtatc atccagggt 
      gtttccgtt gttgcattt gtaacgaat gcttagacg 
      gtgtgcctt tcactgtgg acgtcgaca ataaaattt 
      attgcgtca atgattacc acaggatgt tcgccccga 
      aatgtgctt cgtgtggca aaggtataa caccagttg 
      agggtacag aagaaaccg tccgagtgg tctccatgg 
      ataaggact tccacgttg actgttaca tatgcgaag 
      aatgtggaa tgcagttaa ctgacgaac ctgacaagc 
      gttgttatc cacttgagg ggcgtttga tgtgtcgaa 
      gttgtcaca ttgagcgcc tccaacata cccccaggc 
      agatgcatc ctgttgctg ctacatatc agtatacgg 
      gctaaaaat taccgtttt taggttagt cttagccca 
      ccagaaaca aactactta ttttgcttt tattaggaa 
      tttaatgtg ccaggtttg gtagattgt gagggtgta 
      atgatggtt cgctcattt gaaaagtaa ttattaata 
      ggacgagag tatatttgc ttttagtgt gtagcttta 
      atcttgtta ttgtcgttg gacaaggta ggaggaacg 
      ctaaaagtg gtctagagt tttaggaat tgttatgta 
      agttaaata gtaatttta cgacctaaa tgctaaatg 
      tgattggtt ttaatttta attgtatga aacgccatg 
      gttttaatg ggtatgaag tcaacgatg tacagtgca 
      accttgatt tttctggaa aagttcgaa tgatcgagg 
      ttgtactgt aattatttt ccgatttca tgtccgcct 
      aattaacaa ttgcaatta agtgtttag gtgttaagt 
      gtataaact tgttcctaa ggtattttt gtattattt 
      ttgttgtat gaagctttt gccttttgg catctaaaa 
      caattcatt tttatacaa agtttattt aaaatttgc 
      tatgaattg ctgtgatat gttttattg acaggacaa 
      gcttattga ttttcattc catttagtg tttttatgc 
      ttttgtatt ataataaat acatacaca aatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY