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Gene information
General information
TC013877 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 1531863..1536938
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC013877
      iBeetle screen iB_02216
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC013877 RA
        MASGDNRKT KLKCPPKNP CLLIRQLVE LIEHPISQN 
        ATERHLQLL NEFLNASHY AFWLQDIPF IIRLYENIV 
        KRIDKFPQY KQPLECLLY FSAIPPMLT KNSDKLEYV 
        RDFQEYFSV LGYLILCLH DDNFKKLVL NAIRNLLQP 
        KPPIGYCCV PLDYCKEAA EKSRLPEVL AQLLQVVSD 
        QLYPLLLDV MFLLCEISP KVCEEIVAE NAIEYLLQR 
        LEPTWHERP SEIKPRNPS SQDNIPILD DTCCILWKL 
        LNVVDAQKN NVPNPSLFA LWSLQYAFR WSSVCPGHR 
        VNRNNLTAL ILKIMITFP TMEFIESDL GEDIAVLAL 
        AVANCGFRS NLEFSEDEL DFQFNKIIW ACLHFFRQY 
        QGGDEVLKQ SELIAALTK CIFQEHPAT KDHIEEFCE 
        LTKIILRVS PKYLPSSHE KFMEVNGPL KVVKMIEGF 
        LIKTYEMEV LDCYMYCLT EMLLQKNAD LLRILGENN 
        TYDIVASTC WKVLTISKS LTKFNQKVL TRGFTILQL 
        LLSAGYADD KPITLCLDF LNRVIHPNE NDPLIDVRA 
        TICALHLLW QSVVTSNSS YSKFLKKGG IYILLDITE 
        LCAFPAKVV SLGLLADTA EKSPSIPYF ITWRKKGQR 
        LLPMLMEIF RNEKMMTCH SIESQNENV WLVLCDGQK 
        INFRADCLT QEQLLESCR CKVHAILYA LKVRFRESV 
        EVADENYKL FSGKLKTDD ELTMLFAEN YSSLKMCQS 
        WIRVFRELQ SEGVTPVEV DGDHLNEKL ERIRHWKQY 
        LRYKIRQIV QKESQQDHF NELETYTTL KDARLSDAL 
        DGLKELRYI SNCTERMFQ MRKKLKLQR EVEVLRSRN 
        TNFHRTISA GLFTNVYNQ SISIQSNIV FDKKDDQSP 
        PSPVSLDDN QRLSDSGSE HDLSEHGLE C
      CDS sequence
      • >TC013877 RA
        atggcaagt ggtgataac agaaaaaca aagctaaag 
        tgccccccg aaaaatccc tgtctcctc ataagacag 
        ctcgtggag ttaatcgag cacccaatt tcgcaaaat 
        gcaactgag cgtcactta caactgttg aatgagttt 
        ttaaacgcc tcacattac gctttttgg cttcaagac 
        attccgttt ataatccgc ttgtacgaa aatattgtt 
        aaaagaatt gacaaattc ccccagtac aagcaaccc 
        ctcgaatgc ttgctgtac ttcagtgca attccgcca 
        atgttgacg aaaaattcg gacaaactg gaatacgta 
        agagacttt caggagtat ttttcagtc ttgggttat 
        ttaatttta tgcttacat gacgacaat ttcaaaaaa 
        ctggtctta aacgcaatc aggaattta ttacaaccc 
        aaaccaccg atcggctac tgttgcgtt cctcttgat 
        tattgtaaa gaagcggct gaaaaaagc cggcttccg 
        gaagtttta gcgcaacta ttgcaagtc gtttctgat 
        caattatat cccttgctg ctggacgta atgttcctc 
        ctttgcgaa atttcgccg aaagtttgc gaggaaata 
        gtcgctgaa aatgcgatt gagtatctt ttgcaacga 
        ctggagcca acttggcac gagcggcct tcggaaatc 
        aagcccaga aacccatct tcgcaagac aatattcct 
        atcctggac gacacttgt tgcatttta tggaaattg 
        ttaaatgtg gtcgatgcg cagaaaaac aacgttcca 
        aacccgtca cttttcgcg ttatggagt ttgcaatac 
        gcttttcgg tggtcaagc gtctgtccg ggacacaga 
        gtcaatcgg aacaattta acggctctt attttaaaa 
        atcatgatc acctttccc accatggag ttcatcgag 
        agcgatctc ggcgaagat attgcagtt ttggccctg 
        gcggtggca aactgcggg ttccgatcc aacttggag 
        ttctctgaa gacgagctt gattttcaa ttcaacaaa 
        atcatttgg gcttgtttg cacttcttc cggcaatat 
        caggggggt gacgaggtc ttgaaacag agcgaattg 
        attgcagct ctcacgaaa tgcattttc caagaacac 
        ccagcgaca aaggaccac attgaggag ttctgcgaa 
        ctgactaaa atcatactc agagtgagt cccaaatat 
        ttgccgagt tcgcacgaa aaattcatg gaagttaat 
        ggaccactt aaagttgtc aaaatgatc gaaggtttt 
        ttaatcaaa acgtacgaa atggaagtc ctggattgc 
        tacatgtac tgcttgact gaaatgctg ctccagaaa 
        aacgccgat ctgcttcgg attctgggc gaaaacaac 
        acttacgac attgtcgca tccacatgt tggaaagtt 
        ttaacaatc agtaaatcc ctaacaaag ttcaaccaa 
        aaagtccta actcgggga ttcaccatt ttgcagtta 
        cttttatca gccggttac gccgatgat aaacctata 
        accttgtgt ctcgatttt ctgaatcgg gtgattcac 
        ccgaatgaa aatgatccc ttgatcgac gttagagcc 
        acaatctgt gcactgcat ttgttgtgg cagagtgtg 
        gtcacatca aacagctcc tactccaag tttttgaaa 
        aaagggggg atttatatc ctactggac atcaccgag 
        ttgtgtgct ttccctgcc aaagtggtt tcgctgggc 
        cttctggcc gacactgcc gaaaaatcc ccctccatc 
        ccctacttt atcacttgg cgcaaaaaa ggccaaaga 
        ctgctcccg atgttgatg gaaattttc cgaaacgag 
        aaaatgatg acttgccac agcatcgaa agccagaat 
        gaaaatgta tggttggtg ttgtgtgac ggccaaaaa 
        atcaatttc cgcgccgat tgtttgaca caagaacag 
        cttttggaa agctgcaga tgcaaagtt catgctatt 
        ttgtacgct ttgaaagtc aggtttaga gagagcgtt 
        gaagtggcc gatgaaaat tacaagctc ttcagcgga 
        aaattgaaa acagacgac gagctcaca atgctattc 
        gctgaaaac tactcctcg ctgaaaatg tgccagagt 
        tggattcgc gtttttcgc gaattgcag agtgaggga 
        gtaacgcct gttgaggtg gacggtgac cacttgaac 
        gaaaaactc gaaaggatt cggcattgg aaacaatac 
        ctccgatac aaaatcagg caaatcgtc caaaaggag 
        agccaacaa gaccatttc aacgagttg gaaacttac 
        acaactctg aaagacgct cggctgtcc gatgcttta 
        gacgggttg aaagaactg cgttacatt tccaattgt 
        acggagaga atgtttcaa atgcgtaag aaattgaaa 
        ctccaaagg gaagttgag gtcttgcgt tcgcgaaac 
        acgaatttt catcgcact attagcgcg gggctgttt 
        acgaatgtt tacaatcag agtatcagt attcagagt 
        aacattgtt tttgataaa aaagacgac caaagcccg 
        cccagtccc gtctcattg gacgacaat caacgattg 
        agtgactca gggtctgag catgatttg agcgaacat 
        gggttggaa tgctaa
      mRNA sequence
      • >TC013877 RA
        tacagtggg ggctttttt taaactaat ttgacactt 
        cgacttttc gaaaaactt caaaataat ggcaagtgg 
        tgataacag aaaaacaaa gctaaagtg ccccccgaa 
        aaatccctg tctcctcat aagacagct cgtggagtt 
        aatcgagca cccaatttc gcaaaatgc aactgagcg 
        tcacttaca actgttgaa tgagttttt aaacgcctc 
        acattacgc tttttggct tcaagacat tccgtttat 
        aatccgctt gtacgaaaa tattgttaa aagaattga 
        caaattccc ccagtacaa gcaacccct cgaatgctt 
        gctgtactt cagtgcaat tccgccaat gttgacgaa 
        aaattcgga caaactgga atacgtaag agactttca 
        ggagtattt ttcagtctt gggttattt aattttatg 
        cttacatga cgacaattt caaaaaact ggtcttaaa 
        cgcaatcag gaatttatt acaacccaa accaccgat 
        cggctactg ttgcgttcc tcttgatta ttgtaaaga 
        agcggctga aaaaagccg gcttccgga agttttagc 
        gcaactatt gcaagtcgt ttctgatca attatatcc 
        cttgctgct ggacgtaat gttcctcct ttgcgaaat 
        ttcgccgaa agtttgcga ggaaatagt cgctgaaaa 
        tgcgattga gtatctttt gcaacgact ggagccaac 
        ttggcacga gcggccttc ggaaatcaa gcccagaaa 
        cccatcttc gcaagacaa tattcctat cctggacga 
        cacttgttg cattttatg gaaattgtt aaatgtggt 
        cgatgcgca gaaaaacaa cgttccaaa cccgtcact 
        tttcgcgtt atggagttt gcaatacgc ttttcggtg 
        gtcaagcgt ctgtccggg acacagagt caatcggaa 
        caatttaac ggctcttat tttaaaaat catgatcac 
        ctttcccac catggagtt catcgagag cgatctcgg 
        cgaagatat tgcagtttt ggccctggc ggtggcaaa 
        ctgcgggtt ccgatccaa cttggagtt ctctgaaga 
        cgagcttga ttttcaatt caacaaaat catttgggc 
        ttgtttgca cttcttccg gcaatatca ggggggtga 
        cgaggtctt gaaacagag cgaattgat tgcagctct 
        cacgaaatg cattttcca agaacaccc agcgacaaa 
        ggaccacat tgaggagtt ctgcgaact gactaaaat 
        catactcag agtgagtcc caaatattt gccgagttc 
        gcacgaaaa attcatgga agttaatgg accacttaa 
        agttgtcaa aatgatcga aggtttttt aatcaaaac 
        gtacgaaat ggaagtcct ggattgcta catgtactg 
        cttgactga aatgctgct ccagaaaaa cgccgatct 
        gcttcggat tctgggcga aaacaacac ttacgacat 
        tgtcgcatc cacatgttg gaaagtttt aacaatcag 
        taaatccct aacaaagtt caaccaaaa agtcctaac 
        tcggggatt caccatttt gcagttact tttatcagc 
        cggttacgc cgatgataa acctataac cttgtgtct 
        cgattttct gaatcgggt gattcaccc gaatgaaaa 
        tgatccctt gatcgacgt tagagccac aatctgtgc 
        actgcattt gttgtggca gagtgtggt cacatcaaa 
        cagctccta ctccaagtt tttgaaaaa aggggggat 
        ttatatcct actggacat caccgagtt gtgtgcttt 
        ccctgccaa agtggtttc gctgggcct tctggccga 
        cactgccga aaaatcccc ctccatccc ctactttat 
        cacttggcg caaaaaagg ccaaagact gctcccgat 
        gttgatgga aattttccg aaacgagaa aatgatgac 
        ttgccacag catcgaaag ccagaatga aaatgtatg 
        gttggtgtt gtgtgacgg ccaaaaaat caatttccg 
        cgccgattg tttgacaca agaacagct tttggaaag 
        ctgcagatg caaagttca tgctatttt gtacgcttt 
        gaaagtcag gtttagaga gagcgttga agtggccga 
        tgaaaatta caagctctt cagcggaaa attgaaaac 
        agacgacga gctcacaat gctattcgc tgaaaacta 
        ctcctcgct gaaaatgtg ccagagttg gattcgcgt 
        ttttcgcga attgcagag tgagggagt aacgcctgt 
        tgaggtgga cggtgacca cttgaacga aaaactcga 
        aaggattcg gcattggaa acaatacct ccgatacaa 
        aatcaggca aatcgtcca aaaggagag ccaacaaga 
        ccatttcaa cgagttgga aacttacac aactctgaa 
        agacgctcg gctgtccga tgctttaga cgggttgaa 
        agaactgcg ttacatttc caattgtac ggagagaat 
        gtttcaaat gcgtaagaa attgaaact ccaaaggga 
        agttgaggt cttgcgttc gcgaaacac gaattttca 
        tcgcactat tagcgcggg gctgtttac gaatgttta 
        caatcagag tatcagtat tcagagtaa cattgtttt 
        tgataaaaa agacgacca aagcccgcc cagtcccgt 
        ctcattgga cgacaatca acgattgag tgactcagg 
        gtctgagca tgatttgag cgaacatgg gttggaatg 
        ctaattttc attgtataa ataaatgcg aaaaatcac 
        g