| General information | |||
| TC013875 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 2603930..2608737 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013875 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02214 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
| in same well (21) as iB02213 |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
| in the same well as iB2213 (well 21) but all pupae are wildtypic |
| in the same well as iB02213 (#21) but all animals are wildtypic and no significantly increased lethality is observed |
| animals in well 21 together with iB2213 but all animals phenotypically wildtype |
| in the same well as iB2213 (well 21) but all pupae are wildtypic |
| in the same well as iB02213 (#21) but all animals are wildtypic and no significantly increased lethality is observed |
| in the same well as iB02213 (#21) but all animals are wildtypic and no significantly increased lethality is observed |
| animals in well 21 together with iB2213 but all animals phenotypically wildtype |
- >TC013875 RA
MPIRRAADP ITVQHVWDA IRSAVLQRQ MADSKRIIK YLQRVDNCT YAQAELYVQ QTHQDGLIV ALQKVTKTS KGDLVDSFR IPTHEVPPL DGKDWYCFE CHLAGDVTA CQQCFRVFH ADCVPNGRL KFQEYKKTN ILYDQLSKS GVKDESTSN SVNSINISS DSDDLNASE LSTYKFNNN GQMKSDALE YDETLCSMC NVSKLDDGC NLEKSELNY LLKFVIHRI QSWLPHTLT HTMAQQERP EWLTDKELS WRANQLFFE HRDMSVIEV NLNNESYTK LYQFLADVL TIQHNVAIF HGIESQEYG AAELMIRDC MHDLTELKL CVDCYRHSN EKINAKWFC LPCRVPHKL VWAKQKGYP YWPAKVIQE TSTHFDVRF FGGKYERSL LIKSLIKPI ETPQSKLQI KTSSAFNKA VEELRFHQR LMQNPDELQ KLISSSKNR VRSLNKQRI STLKKSLNS SLEVKKRNS FEMNDVYKF EDNPFGQQN LSLTHKRKS SFCEGSPVS KKNSTSSVE KNEEVHNTS DSVIHISDG EDDEYPYYD YSSVSNTNY NGSYEQVSS STENYRFQD RSQENGMQQ LDHPYSDSV EKMRRRLEC VSDKKELIK CAMDCMQSE IDQITNNHN DHLKRLFES HNAQISEIK KKQWCYNCE QEAIYHCCW NTAYCSQSC QQQHWQAEH KKVCRRKR
- >TC013875 RB
MPIRRAADP ITVQHVWDA IRSAVLQRQ MADSKRIIK YLQRVDNCT YAQAELYVQ QTHQDGLIV SDRALQKVT KTSKGDLVD SFRIPTHEV PPLDGKDWY CFECHLAGD VTACQQCFR VFHADCVPN GRLKFQEYK KTNILYDQL SKSGVKDES TSNSVNSIN ISSDSDDLN ASELSTYKF NNNGQMKSD ALEYDETLC SMCNVSKLD DGCNLEKSE LNYLLKFVI HRIQSWLPH TLTHTMAQQ ERPEWLTDK ELSWRANQL FFEHRDMSV IEVNLNNES YTKLYQFLA DVLTIQHNV AIFHGIESQ EYGAAELMI RDCMHDLTE LKLCVDCYR HSNEKINAK WFCLPCRVP HKLVWAKQK GYPYWPAKV IQETSTHFD VRFFGGKYE RSLLIKSLI KPIETPQSK LQIKTSSAF NKAVEELRF HQRLMQNPD ELQKLISSS KNRVRSLNK QRISTLKKS LNSSLEVKK RNSFEMNDV YKFEDNPFG QQNLSLTHK RKSSFCEGS PVSKKNSTS SVEKNEEVH NTSDSVIHI SDGEDDEYP YYDYSSVSN TNYNGSYEQ VSSSTENYR FQDRSQENG MQQLDHPYS DSVEKMRRR LECVSDKKE LIKCAMDCM QSEIDQITN NHNDHLKRL FESHNAQIS EIKKKQWCY NCEQEAIYH CCWNTAYCS QSCQQQHWQ AEHKKVCRR KR
- >TC013875 RA
atgcctatc agaagagca gccgatccg atcaccgtc caacatgtg tgggatgca attcgatcg gcggttctg cagcgccaa atggccgat tcgaaacga ataataaaa tatttgcaa cgcgtggac aactgtacc tacgcccaa gccgaatta tacgtgcaa cagacgcat caagacgga ctgattgtg gccttacag aaagtaacc aaaacgtca aaaggggac ttggtggac agctttcgg atacctaca cacgaagtg ccgcccttg gacgggaaa gactggtat tgcttcgaa tgccacctc gccggtgat gtgaccgcc tgccaacag tgtttccgc gttttccat gcggactgt gtcccaaac ggccgtctc aagttccag gaatataag aaaactaat attctttat gaccagttg agtaaatca ggggtgaag gatgagtcg acgagtaat tcagtgaat tcgattaat atttcgtcg gattccgac gacttgaat gcgagtgag ttgagtact tacaaattt aataacaac ggacaaatg aaaagtgat gcgcttgag tatgacgaa acgttgtgc agcatgtgc aacgtttcc aagctggac gatggctgt aatttagaa aagtctgaa ctgaattat ctccttaaa tttgtaata cacaggatt cagtcttgg ttgccgcac actttaaca cacaccatg gcccagcaa gagcggccc gagtggcta acagacaag gagctgagt tggcgggca aaccagcta tttttcgaa cacagagac atgtccgtc attgaggtg aacctaaat aacgagtcg tacacgaaa ctgtaccaa tttctcgcc gatgtttta acaatccaa cacaacgtt gcgattttt cacggaatt gagtcgcag gaatacggg gctgcggaa cttatgatt agagactgc atgcacgac ctgaccgag ttgaaactt tgcgttgat tgttaccgc catagtaat gagaaaatt aacgctaag tggttctgt ttgccgtgc agagtgcct cataagtta gtttgggcc aaacaaaag ggctacccc tattggcct gctaaagtg attcaagag acgagcacg cattttgat gttaggttt tttgggggt aaatacgaa aggtcgctt ctgatcaaa tcgttaatt aaaccgatt gaaacgccg caatcgaag ttgcaaata aagacgtct tcggcgttt aacaaggcg gtggaggag ttaaggttc catcagcgc ttgatgcag aaccctgac gagctccag aagttgatc agttcgtcg aaaaacagg gtcaggagt ttaaataag cagcgaatt tcgacgttg aagaagtcg cttaatagt tcactggaa gtgaaaaag agaaattca tttgaaatg aatgatgtt tacaagttt gaggataat ccattcggt cagcaaaat ttaagccta acgcataaa cgtaaaagc agtttttgt gaaggaagt cccgtgtcg aaaaaaaat tcaactagt tccgtggag aaaaacgag gaagttcac aatacttcc gatagcgtt atacacata tcagacggc gaagacgat gagtatccc tattacgat tattcatcg gtttctaat acaaactat aatggttca tatgagcaa gtttcaagt tccactgaa aattacaga ttccaggac agaagccag gaaaatggg atgcagcaa cttgatcac ccttatagt gattcagtt gaaaagatg agaagaaga cttgaatgt gtgagtgat aaaaaagag cttataaag tgtgcaatg gactgtatg caaagtgag attgatcaa attacaaat aatcacaat gatcatttg aaaaggttg tttgagtcc cacaatgcg cagatatcg gagattaag aaaaagcaa tggtgctat aattgcgaa caggaagcc atttatcat tgttgctgg aacacggcg tattgttcc caaagttgt caacaacag cactggcaa gcagaacat aaaaaagtt tgcaggcgt aagcgctag
- >TC013875 RB
atgcctatc agaagagca gccgatccg atcaccgtc caacatgtg tgggatgca attcgatcg gcggttctg cagcgccaa atggccgat tcgaaacga ataataaaa tatttgcaa cgcgtggac aactgtacc tacgcccaa gccgaatta tacgtgcaa cagacgcat caagacgga ctgattgtc tccgacagg gccttacag aaagtaacc aaaacgtca aaaggggac ttggtggac agctttcgg atacctaca cacgaagtg ccgcccttg gacgggaaa gactggtat tgcttcgaa tgccacctc gccggtgat gtgaccgcc tgccaacag tgtttccgc gttttccat gcggactgt gtcccaaac ggccgtctc aagttccag gaatataag aaaactaat attctttat gaccagttg agtaaatca ggggtgaag gatgagtcg acgagtaat tcagtgaat tcgattaat atttcgtcg gattccgac gacttgaat gcgagtgag ttgagtact tacaaattt aataacaac ggacaaatg aaaagtgat gcgcttgag tatgacgaa acgttgtgc agcatgtgc aacgtttcc aagctggac gatggctgt aatttagaa aagtctgaa ctgaattat ctccttaaa tttgtaata cacaggatt cagtcttgg ttgccgcac actttaaca cacaccatg gcccagcaa gagcggccc gagtggcta acagacaag gagctgagt tggcgggca aaccagcta tttttcgaa cacagagac atgtccgtc attgaggtg aacctaaat aacgagtcg tacacgaaa ctgtaccaa tttctcgcc gatgtttta acaatccaa cacaacgtt gcgattttt cacggaatt gagtcgcag gaatacggg gctgcggaa cttatgatt agagactgc atgcacgac ctgaccgag ttgaaactt tgcgttgat tgttaccgc catagtaat gagaaaatt aacgctaag tggttctgt ttgccgtgc agagtgcct cataagtta gtttgggcc aaacaaaag ggctacccc tattggcct gctaaagtg attcaagag acgagcacg cattttgat gttaggttt tttgggggt aaatacgaa aggtcgctt ctgatcaaa tcgttaatt aaaccgatt gaaacgccg caatcgaag ttgcaaata aagacgtct tcggcgttt aacaaggcg gtggaggag ttaaggttc catcagcgc ttgatgcag aaccctgac gagctccag aagttgatc agttcgtcg aaaaacagg gtcaggagt ttaaataag cagcgaatt tcgacgttg aagaagtcg cttaatagt tcactggaa gtgaaaaag agaaattca tttgaaatg aatgatgtt tacaagttt gaggataat ccattcggt cagcaaaat ttaagccta acgcataaa cgtaaaagc agtttttgt gaaggaagt cccgtgtcg aaaaaaaat tcaactagt tccgtggag aaaaacgag gaagttcac aatacttcc gatagcgtt atacacata tcagacggc gaagacgat gagtatccc tattacgat tattcatcg gtttctaat acaaactat aatggttca tatgagcaa gtttcaagt tccactgaa aattacaga ttccaggac agaagccag gaaaatggg atgcagcaa cttgatcac ccttatagt gattcagtt gaaaagatg agaagaaga cttgaatgt gtgagtgat aaaaaagag cttataaag tgtgcaatg gactgtatg caaagtgag attgatcaa attacaaat aatcacaat gatcatttg aaaaggttg tttgagtcc cacaatgcg cagatatcg gagattaag aaaaagcaa tggtgctat aattgcgaa caggaagcc atttatcat tgttgctgg aacacggcg tattgttcc caaagttgt caacaacag cactggcaa gcagaacat aaaaaagtt tgcaggcgt aagcgctag
- >TC013875 RA
acttatcaa aatactttc caaatttcg aaaattatc aaaatggat tttgaggcc gccgttttt gtttgtaaa ttgcaggta tgcctatca gaagagcag ccgatccga tcaccgtcc aacatgtgt gggatgcaa ttcgatcgg cggttctgc agcgccaaa tggccgatt cgaaacgaa taataaaat atttgcaac gcgtggaca actgtacct acgcccaag ccgaattat acgtgcaac agacgcatc aagacggac tgattgtgg ccttacaga aagtaacca aaacgtcaa aaggggact tggtggaca gctttcgga tacctacac acgaagtgc cgcccttgg acgggaaag actggtatt gcttcgaat gccacctcg ccggtgatg tgaccgcct gccaacagt gtttccgcg ttttccatg cggactgtg tcccaaacg gccgtctca agttccagg aatataaga aaactaata ttctttatg accagttga gtaaatcag gggtgaagg atgagtcga cgagtaatt cagtgaatt cgattaata tttcgtcgg attccgacg acttgaatg cgagtgagt tgagtactt acaaattta ataacaacg gacaaatga aaagtgatg cgcttgagt atgacgaaa cgttgtgca gcatgtgca acgtttcca agctggacg atggctgta atttagaaa agtctgaac tgaattatc tccttaaat ttgtaatac acaggattc agtcttggt tgccgcaca ctttaacac acaccatgg cccagcaag agcggcccg agtggctaa cagacaagg agctgagtt ggcgggcaa accagctat ttttcgaac acagagaca tgtccgtca ttgaggtga acctaaata acgagtcgt acacgaaac tgtaccaat ttctcgccg atgttttaa caatccaac acaacgttg cgatttttc acggaattg agtcgcagg aatacgggg ctgcggaac ttatgatta gagactgca tgcacgacc tgaccgagt tgaaacttt gcgttgatt gttaccgcc atagtaatg agaaaatta acgctaagt ggttctgtt tgccgtgca gagtgcctc ataagttag tttgggcca aacaaaagg gctacccct attggcctg ctaaagtga ttcaagaga cgagcacgc attttgatg ttaggtttt ttgggggta aatacgaaa ggtcgcttc tgatcaaat cgttaatta aaccgattg aaacgccgc aatcgaagt tgcaaataa agacgtctt cggcgttta acaaggcgg tggaggagt taaggttcc atcagcgct tgatgcaga accctgacg agctccaga agttgatca gttcgtcga aaaacaggg tcaggagtt taaataagc agcgaattt cgacgttga agaagtcgc ttaatagtt cactggaag tgaaaaaga gaaattcat ttgaaatga atgatgttt acaagtttg aggataatc cattcggtc agcaaaatt taagcctaa cgcataaac gtaaaagca gtttttgtg aaggaagtc ccgtgtcga aaaaaaatt caactagtt ccgtggaga aaaacgagg aagttcaca atacttccg atagcgtta tacacatat cagacggcg aagacgatg agtatccct attacgatt attcatcgg tttctaata caaactata atggttcat atgagcaag tttcaagtt ccactgaaa attacagat tccaggaca gaagccagg aaaatggga tgcagcaac ttgatcacc cttatagtg attcagttg aaaagatga gaagaagac ttgaatgtg tgagtgata aaaaagagc ttataaagt gtgcaatgg actgtatgc aaagtgaga ttgatcaaa ttacaaata atcacaatg atcatttga aaaggttgt ttgagtccc acaatgcgc agatatcgg agattaaga aaaagcaat ggtgctata attgcgaac aggaagcca tttatcatt gttgctgga acacggcgt attgttccc aaagttgtc aacaacagc actggcaag cagaacata aaaaagttt gcaggcgta agcgctagt ttcatacaa aaattaaaa aatgttatt ttgtgtatt atctatttt tatagtttg tttatcctc taatatcat tatgtagag taatgggaa cattggcgt aattttcat tgttttggt actttttag gaccaagtt acgaaagta caattctag ttaactttt taattgtag gcaataact tgagttgta agagtttat cacatagtg ttgttgaaa cttccaaat catttttaa acgttgtat cacaattga aagttatgc cactacttc attgtttgt tcttatttt atggtttaa aata
- >TC013875 RB
ttctcgatg ctcttcatt ttgttggta tcaaaatta ttaaaatcc atacaagcc cgtggtatt gtcaaaatc gtaaactaa aaattgtcg taatttgtt tgtgaacgg atagatgct tcgttttgt cgaatccgc gagtccagg aagtcttaa agtaaaggt atgcctatc agaagagca gccgatccg atcaccgtc caacatgtg tgggatgca attcgatcg gcggttctg cagcgccaa atggccgat tcgaaacga ataataaaa tatttgcaa cgcgtggac aactgtacc tacgcccaa gccgaatta tacgtgcaa cagacgcat caagacgga ctgattgtc tccgacagg gccttacag aaagtaacc aaaacgtca aaaggggac ttggtggac agctttcgg atacctaca cacgaagtg ccgcccttg gacgggaaa gactggtat tgcttcgaa tgccacctc gccggtgat gtgaccgcc tgccaacag tgtttccgc gttttccat gcggactgt gtcccaaac ggccgtctc aagttccag gaatataag aaaactaat attctttat gaccagttg agtaaatca ggggtgaag gatgagtcg acgagtaat tcagtgaat tcgattaat atttcgtcg gattccgac gacttgaat gcgagtgag ttgagtact tacaaattt aataacaac ggacaaatg aaaagtgat gcgcttgag tatgacgaa acgttgtgc agcatgtgc aacgtttcc aagctggac gatggctgt aatttagaa aagtctgaa ctgaattat ctccttaaa tttgtaata cacaggatt cagtcttgg ttgccgcac actttaaca cacaccatg gcccagcaa gagcggccc gagtggcta acagacaag gagctgagt tggcgggca aaccagcta tttttcgaa cacagagac atgtccgtc attgaggtg aacctaaat aacgagtcg tacacgaaa ctgtaccaa tttctcgcc gatgtttta acaatccaa cacaacgtt gcgattttt cacggaatt gagtcgcag gaatacggg gctgcggaa cttatgatt agagactgc atgcacgac ctgaccgag ttgaaactt tgcgttgat tgttaccgc catagtaat gagaaaatt aacgctaag tggttctgt ttgccgtgc agagtgcct cataagtta gtttgggcc aaacaaaag ggctacccc tattggcct gctaaagtg attcaagag acgagcacg cattttgat gttaggttt tttgggggt aaatacgaa aggtcgctt ctgatcaaa tcgttaatt aaaccgatt gaaacgccg caatcgaag ttgcaaata aagacgtct tcggcgttt aacaaggcg gtggaggag ttaaggttc catcagcgc ttgatgcag aaccctgac gagctccag aagttgatc agttcgtcg aaaaacagg gtcaggagt ttaaataag cagcgaatt tcgacgttg aagaagtcg cttaatagt tcactggaa gtgaaaaag agaaattca tttgaaatg aatgatgtt tacaagttt gaggataat ccattcggt cagcaaaat ttaagccta acgcataaa cgtaaaagc agtttttgt gaaggaagt cccgtgtcg aaaaaaaat tcaactagt tccgtggag aaaaacgag gaagttcac aatacttcc gatagcgtt atacacata tcagacggc gaagacgat gagtatccc tattacgat tattcatcg gtttctaat acaaactat aatggttca tatgagcaa gtttcaagt tccactgaa aattacaga ttccaggac agaagccag gaaaatggg atgcagcaa cttgatcac ccttatagt gattcagtt gaaaagatg agaagaaga cttgaatgt gtgagtgat aaaaaagag cttataaag tgtgcaatg gactgtatg caaagtgag attgatcaa attacaaat aatcacaat gatcatttg aaaaggttg tttgagtcc cacaatgcg cagatatcg gagattaag aaaaagcaa tggtgctat aattgcgaa caggaagcc atttatcat tgttgctgg aacacggcg tattgttcc caaagttgt caacaacag cactggcaa gcagaacat aaaaaagtt tgcaggcgt aagcgctag tttcataca aaaattaaa aaatgttat tttgtgtat tatctattt ttatagttt gtttatcct ctaatatca ttatgtaga gtaatggga acattggcg taattttca ttgttttgg tacttttta ggaccaagt tacgaaagt acaattcta gttaacttt ttaattgta ggcaataac ttgagttgt aagagttta tcacatagt gttgttgaa acttccaaa tcattttta aacgttgta tcacaattg aaagttatg ccactactt cattgtttg ttcttattt tatggttta aaata
