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Gene information
General information
TC013875 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 1568722..1573529
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013875
    iBeetle screen iB_02214
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    TR: 134957 potentially confused/crosscontaminated with : potentially confused/contaminated with, iB # (comma separated) 2213
    in same well (21) as iB02213
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    potentially confused/crosscontaminated with: iB # (comma separated) 2213
    in the same well as iB2213 (well 21) but all pupae are wildtypic
    potentially confused/crosscontaminated with: iB # (comma separated) 2213
    in the same well as iB02213 (#21) but all animals are wildtypic and no significantly increased lethality is observed
    potentially confused/crosscontaminated with: iB # (comma separated) 2213
    animals in well 21 together with iB2213 but all animals phenotypically wildtype
    Pupal morphology
    potentially confused/crosscontaminated with: iB # (comma separated) 2213
    in the same well as iB2213 (well 21) but all pupae are wildtypic
    potentially confused/crosscontaminated with: iB # (comma separated) 2213
    in the same well as iB02213 (#21) but all animals are wildtypic and no significantly increased lethality is observed
    Adult morphology
    potentially confused/crosscontaminated with: iB # (comma separated) 2213
    in the same well as iB02213 (#21) but all animals are wildtypic and no significantly increased lethality is observed
    potentially confused/crosscontaminated with: iB # (comma separated) 2213
    animals in well 21 together with iB2213 but all animals phenotypically wildtype
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013875 RA
      MPIRRAADP ITVQHVWDA IRSAVLQRQ MADSKRIIK 
      YLQRVDNCT YAQAELYVQ QTHQDGLIV ALQKVTKTS 
      KGDLVDSFR IPTHEVPPL DGKDWYCFE CHLAGDVTA 
      CQQCFRVFH ADCVPNGRL KFQEYKKTN ILYDQLSKS 
      GVKDESTSN SVNSINISS DSDDLNASE LSTYKFNNN 
      GQMKSDALE YDETLCSMC NVSKLDDGC NLEKSELNY 
      LLKFVIHRI QSWLPHTLT HTMAQQERP EWLTDKELS 
      WRANQLFFE HRDMSVIEV NLNNESYTK LYQFLADVL 
      TIQHNVAIF HGIESQEYG AAELMIRDC MHDLTELKL 
      CVDCYRHSN EKINAKWFC LPCRVPHKL VWAKQKGYP 
      YWPAKVIQE TSTHFDVRF FGGKYERSL LIKSLIKPI 
      ETPQSKLQI KTSSAFNKA VEELRFHQR LMQNPDELQ 
      KLISSSKNR VRSLNKQRI STLKKSLNS SLEVKKRNS 
      FEMNDVYKF EDNPFGQQN LSLTHKRKS SFCEGSPVS 
      KKNSTSSVE KNEEVHNTS DSVIHISDG EDDEYPYYD 
      YSSVSNTNY NGSYEQVSS STENYRFQD RSQENGMQQ 
      LDHPYSDSV EKMRRRLEC VSDKKELIK CAMDCMQSE 
      IDQITNNHN DHLKRLFES HNAQISEIK KKQWCYNCE 
      QEAIYHCCW NTAYCSQSC QQQHWQAEH KKVCRRKR
    • >TC013875 RB
      MPIRRAADP ITVQHVWDA IRSAVLQRQ MADSKRIIK 
      YLQRVDNCT YAQAELYVQ QTHQDGLIV SDRALQKVT 
      KTSKGDLVD SFRIPTHEV PPLDGKDWY CFECHLAGD 
      VTACQQCFR VFHADCVPN GRLKFQEYK KTNILYDQL 
      SKSGVKDES TSNSVNSIN ISSDSDDLN ASELSTYKF 
      NNNGQMKSD ALEYDETLC SMCNVSKLD DGCNLEKSE 
      LNYLLKFVI HRIQSWLPH TLTHTMAQQ ERPEWLTDK 
      ELSWRANQL FFEHRDMSV IEVNLNNES YTKLYQFLA 
      DVLTIQHNV AIFHGIESQ EYGAAELMI RDCMHDLTE 
      LKLCVDCYR HSNEKINAK WFCLPCRVP HKLVWAKQK 
      GYPYWPAKV IQETSTHFD VRFFGGKYE RSLLIKSLI 
      KPIETPQSK LQIKTSSAF NKAVEELRF HQRLMQNPD 
      ELQKLISSS KNRVRSLNK QRISTLKKS LNSSLEVKK 
      RNSFEMNDV YKFEDNPFG QQNLSLTHK RKSSFCEGS 
      PVSKKNSTS SVEKNEEVH NTSDSVIHI SDGEDDEYP 
      YYDYSSVSN TNYNGSYEQ VSSSTENYR FQDRSQENG 
      MQQLDHPYS DSVEKMRRR LECVSDKKE LIKCAMDCM 
      QSEIDQITN NHNDHLKRL FESHNAQIS EIKKKQWCY 
      NCEQEAIYH CCWNTAYCS QSCQQQHWQ AEHKKVCRR 
      KR
    CDS sequence
    • >TC013875 RA
      atgcctatc agaagagca gccgatccg atcaccgtc 
      caacatgtg tgggatgca attcgatcg gcggttctg 
      cagcgccaa atggccgat tcgaaacga ataataaaa 
      tatttgcaa cgcgtggac aactgtacc tacgcccaa 
      gccgaatta tacgtgcaa cagacgcat caagacgga 
      ctgattgtg gccttacag aaagtaacc aaaacgtca 
      aaaggggac ttggtggac agctttcgg atacctaca 
      cacgaagtg ccgcccttg gacgggaaa gactggtat 
      tgcttcgaa tgccacctc gccggtgat gtgaccgcc 
      tgccaacag tgtttccgc gttttccat gcggactgt 
      gtcccaaac ggccgtctc aagttccag gaatataag 
      aaaactaat attctttat gaccagttg agtaaatca 
      ggggtgaag gatgagtcg acgagtaat tcagtgaat 
      tcgattaat atttcgtcg gattccgac gacttgaat 
      gcgagtgag ttgagtact tacaaattt aataacaac 
      ggacaaatg aaaagtgat gcgcttgag tatgacgaa 
      acgttgtgc agcatgtgc aacgtttcc aagctggac 
      gatggctgt aatttagaa aagtctgaa ctgaattat 
      ctccttaaa tttgtaata cacaggatt cagtcttgg 
      ttgccgcac actttaaca cacaccatg gcccagcaa 
      gagcggccc gagtggcta acagacaag gagctgagt 
      tggcgggca aaccagcta tttttcgaa cacagagac 
      atgtccgtc attgaggtg aacctaaat aacgagtcg 
      tacacgaaa ctgtaccaa tttctcgcc gatgtttta 
      acaatccaa cacaacgtt gcgattttt cacggaatt 
      gagtcgcag gaatacggg gctgcggaa cttatgatt 
      agagactgc atgcacgac ctgaccgag ttgaaactt 
      tgcgttgat tgttaccgc catagtaat gagaaaatt 
      aacgctaag tggttctgt ttgccgtgc agagtgcct 
      cataagtta gtttgggcc aaacaaaag ggctacccc 
      tattggcct gctaaagtg attcaagag acgagcacg 
      cattttgat gttaggttt tttgggggt aaatacgaa 
      aggtcgctt ctgatcaaa tcgttaatt aaaccgatt 
      gaaacgccg caatcgaag ttgcaaata aagacgtct 
      tcggcgttt aacaaggcg gtggaggag ttaaggttc 
      catcagcgc ttgatgcag aaccctgac gagctccag 
      aagttgatc agttcgtcg aaaaacagg gtcaggagt 
      ttaaataag cagcgaatt tcgacgttg aagaagtcg 
      cttaatagt tcactggaa gtgaaaaag agaaattca 
      tttgaaatg aatgatgtt tacaagttt gaggataat 
      ccattcggt cagcaaaat ttaagccta acgcataaa 
      cgtaaaagc agtttttgt gaaggaagt cccgtgtcg 
      aaaaaaaat tcaactagt tccgtggag aaaaacgag 
      gaagttcac aatacttcc gatagcgtt atacacata 
      tcagacggc gaagacgat gagtatccc tattacgat 
      tattcatcg gtttctaat acaaactat aatggttca 
      tatgagcaa gtttcaagt tccactgaa aattacaga 
      ttccaggac agaagccag gaaaatggg atgcagcaa 
      cttgatcac ccttatagt gattcagtt gaaaagatg 
      agaagaaga cttgaatgt gtgagtgat aaaaaagag 
      cttataaag tgtgcaatg gactgtatg caaagtgag 
      attgatcaa attacaaat aatcacaat gatcatttg 
      aaaaggttg tttgagtcc cacaatgcg cagatatcg 
      gagattaag aaaaagcaa tggtgctat aattgcgaa 
      caggaagcc atttatcat tgttgctgg aacacggcg 
      tattgttcc caaagttgt caacaacag cactggcaa 
      gcagaacat aaaaaagtt tgcaggcgt aagcgctag
    • >TC013875 RB
      atgcctatc agaagagca gccgatccg atcaccgtc 
      caacatgtg tgggatgca attcgatcg gcggttctg 
      cagcgccaa atggccgat tcgaaacga ataataaaa 
      tatttgcaa cgcgtggac aactgtacc tacgcccaa 
      gccgaatta tacgtgcaa cagacgcat caagacgga 
      ctgattgtc tccgacagg gccttacag aaagtaacc 
      aaaacgtca aaaggggac ttggtggac agctttcgg 
      atacctaca cacgaagtg ccgcccttg gacgggaaa 
      gactggtat tgcttcgaa tgccacctc gccggtgat 
      gtgaccgcc tgccaacag tgtttccgc gttttccat 
      gcggactgt gtcccaaac ggccgtctc aagttccag 
      gaatataag aaaactaat attctttat gaccagttg 
      agtaaatca ggggtgaag gatgagtcg acgagtaat 
      tcagtgaat tcgattaat atttcgtcg gattccgac 
      gacttgaat gcgagtgag ttgagtact tacaaattt 
      aataacaac ggacaaatg aaaagtgat gcgcttgag 
      tatgacgaa acgttgtgc agcatgtgc aacgtttcc 
      aagctggac gatggctgt aatttagaa aagtctgaa 
      ctgaattat ctccttaaa tttgtaata cacaggatt 
      cagtcttgg ttgccgcac actttaaca cacaccatg 
      gcccagcaa gagcggccc gagtggcta acagacaag 
      gagctgagt tggcgggca aaccagcta tttttcgaa 
      cacagagac atgtccgtc attgaggtg aacctaaat 
      aacgagtcg tacacgaaa ctgtaccaa tttctcgcc 
      gatgtttta acaatccaa cacaacgtt gcgattttt 
      cacggaatt gagtcgcag gaatacggg gctgcggaa 
      cttatgatt agagactgc atgcacgac ctgaccgag 
      ttgaaactt tgcgttgat tgttaccgc catagtaat 
      gagaaaatt aacgctaag tggttctgt ttgccgtgc 
      agagtgcct cataagtta gtttgggcc aaacaaaag 
      ggctacccc tattggcct gctaaagtg attcaagag 
      acgagcacg cattttgat gttaggttt tttgggggt 
      aaatacgaa aggtcgctt ctgatcaaa tcgttaatt 
      aaaccgatt gaaacgccg caatcgaag ttgcaaata 
      aagacgtct tcggcgttt aacaaggcg gtggaggag 
      ttaaggttc catcagcgc ttgatgcag aaccctgac 
      gagctccag aagttgatc agttcgtcg aaaaacagg 
      gtcaggagt ttaaataag cagcgaatt tcgacgttg 
      aagaagtcg cttaatagt tcactggaa gtgaaaaag 
      agaaattca tttgaaatg aatgatgtt tacaagttt 
      gaggataat ccattcggt cagcaaaat ttaagccta 
      acgcataaa cgtaaaagc agtttttgt gaaggaagt 
      cccgtgtcg aaaaaaaat tcaactagt tccgtggag 
      aaaaacgag gaagttcac aatacttcc gatagcgtt 
      atacacata tcagacggc gaagacgat gagtatccc 
      tattacgat tattcatcg gtttctaat acaaactat 
      aatggttca tatgagcaa gtttcaagt tccactgaa 
      aattacaga ttccaggac agaagccag gaaaatggg 
      atgcagcaa cttgatcac ccttatagt gattcagtt 
      gaaaagatg agaagaaga cttgaatgt gtgagtgat 
      aaaaaagag cttataaag tgtgcaatg gactgtatg 
      caaagtgag attgatcaa attacaaat aatcacaat 
      gatcatttg aaaaggttg tttgagtcc cacaatgcg 
      cagatatcg gagattaag aaaaagcaa tggtgctat 
      aattgcgaa caggaagcc atttatcat tgttgctgg 
      aacacggcg tattgttcc caaagttgt caacaacag 
      cactggcaa gcagaacat aaaaaagtt tgcaggcgt 
      aagcgctag
    mRNA sequence
    • >TC013875 RA
      acttatcaa aatactttc caaatttcg aaaattatc 
      aaaatggat tttgaggcc gccgttttt gtttgtaaa 
      ttgcaggta tgcctatca gaagagcag ccgatccga 
      tcaccgtcc aacatgtgt gggatgcaa ttcgatcgg 
      cggttctgc agcgccaaa tggccgatt cgaaacgaa 
      taataaaat atttgcaac gcgtggaca actgtacct 
      acgcccaag ccgaattat acgtgcaac agacgcatc 
      aagacggac tgattgtgg ccttacaga aagtaacca 
      aaacgtcaa aaggggact tggtggaca gctttcgga 
      tacctacac acgaagtgc cgcccttgg acgggaaag 
      actggtatt gcttcgaat gccacctcg ccggtgatg 
      tgaccgcct gccaacagt gtttccgcg ttttccatg 
      cggactgtg tcccaaacg gccgtctca agttccagg 
      aatataaga aaactaata ttctttatg accagttga 
      gtaaatcag gggtgaagg atgagtcga cgagtaatt 
      cagtgaatt cgattaata tttcgtcgg attccgacg 
      acttgaatg cgagtgagt tgagtactt acaaattta 
      ataacaacg gacaaatga aaagtgatg cgcttgagt 
      atgacgaaa cgttgtgca gcatgtgca acgtttcca 
      agctggacg atggctgta atttagaaa agtctgaac 
      tgaattatc tccttaaat ttgtaatac acaggattc 
      agtcttggt tgccgcaca ctttaacac acaccatgg 
      cccagcaag agcggcccg agtggctaa cagacaagg 
      agctgagtt ggcgggcaa accagctat ttttcgaac 
      acagagaca tgtccgtca ttgaggtga acctaaata 
      acgagtcgt acacgaaac tgtaccaat ttctcgccg 
      atgttttaa caatccaac acaacgttg cgatttttc 
      acggaattg agtcgcagg aatacgggg ctgcggaac 
      ttatgatta gagactgca tgcacgacc tgaccgagt 
      tgaaacttt gcgttgatt gttaccgcc atagtaatg 
      agaaaatta acgctaagt ggttctgtt tgccgtgca 
      gagtgcctc ataagttag tttgggcca aacaaaagg 
      gctacccct attggcctg ctaaagtga ttcaagaga 
      cgagcacgc attttgatg ttaggtttt ttgggggta 
      aatacgaaa ggtcgcttc tgatcaaat cgttaatta 
      aaccgattg aaacgccgc aatcgaagt tgcaaataa 
      agacgtctt cggcgttta acaaggcgg tggaggagt 
      taaggttcc atcagcgct tgatgcaga accctgacg 
      agctccaga agttgatca gttcgtcga aaaacaggg 
      tcaggagtt taaataagc agcgaattt cgacgttga 
      agaagtcgc ttaatagtt cactggaag tgaaaaaga 
      gaaattcat ttgaaatga atgatgttt acaagtttg 
      aggataatc cattcggtc agcaaaatt taagcctaa 
      cgcataaac gtaaaagca gtttttgtg aaggaagtc 
      ccgtgtcga aaaaaaatt caactagtt ccgtggaga 
      aaaacgagg aagttcaca atacttccg atagcgtta 
      tacacatat cagacggcg aagacgatg agtatccct 
      attacgatt attcatcgg tttctaata caaactata 
      atggttcat atgagcaag tttcaagtt ccactgaaa 
      attacagat tccaggaca gaagccagg aaaatggga 
      tgcagcaac ttgatcacc cttatagtg attcagttg 
      aaaagatga gaagaagac ttgaatgtg tgagtgata 
      aaaaagagc ttataaagt gtgcaatgg actgtatgc 
      aaagtgaga ttgatcaaa ttacaaata atcacaatg 
      atcatttga aaaggttgt ttgagtccc acaatgcgc 
      agatatcgg agattaaga aaaagcaat ggtgctata 
      attgcgaac aggaagcca tttatcatt gttgctgga 
      acacggcgt attgttccc aaagttgtc aacaacagc 
      actggcaag cagaacata aaaaagttt gcaggcgta 
      agcgctagt ttcatacaa aaattaaaa aatgttatt 
      ttgtgtatt atctatttt tatagtttg tttatcctc 
      taatatcat tatgtagag taatgggaa cattggcgt 
      aattttcat tgttttggt actttttag gaccaagtt 
      acgaaagta caattctag ttaactttt taattgtag 
      gcaataact tgagttgta agagtttat cacatagtg 
      ttgttgaaa cttccaaat catttttaa acgttgtat 
      cacaattga aagttatgc cactacttc attgtttgt 
      tcttatttt atggtttaa aata
    • >TC013875 RB
      ttctcgatg ctcttcatt ttgttggta tcaaaatta 
      ttaaaatcc atacaagcc cgtggtatt gtcaaaatc 
      gtaaactaa aaattgtcg taatttgtt tgtgaacgg 
      atagatgct tcgttttgt cgaatccgc gagtccagg 
      aagtcttaa agtaaaggt atgcctatc agaagagca 
      gccgatccg atcaccgtc caacatgtg tgggatgca 
      attcgatcg gcggttctg cagcgccaa atggccgat 
      tcgaaacga ataataaaa tatttgcaa cgcgtggac 
      aactgtacc tacgcccaa gccgaatta tacgtgcaa 
      cagacgcat caagacgga ctgattgtc tccgacagg 
      gccttacag aaagtaacc aaaacgtca aaaggggac 
      ttggtggac agctttcgg atacctaca cacgaagtg 
      ccgcccttg gacgggaaa gactggtat tgcttcgaa 
      tgccacctc gccggtgat gtgaccgcc tgccaacag 
      tgtttccgc gttttccat gcggactgt gtcccaaac 
      ggccgtctc aagttccag gaatataag aaaactaat 
      attctttat gaccagttg agtaaatca ggggtgaag 
      gatgagtcg acgagtaat tcagtgaat tcgattaat 
      atttcgtcg gattccgac gacttgaat gcgagtgag 
      ttgagtact tacaaattt aataacaac ggacaaatg 
      aaaagtgat gcgcttgag tatgacgaa acgttgtgc 
      agcatgtgc aacgtttcc aagctggac gatggctgt 
      aatttagaa aagtctgaa ctgaattat ctccttaaa 
      tttgtaata cacaggatt cagtcttgg ttgccgcac 
      actttaaca cacaccatg gcccagcaa gagcggccc 
      gagtggcta acagacaag gagctgagt tggcgggca 
      aaccagcta tttttcgaa cacagagac atgtccgtc 
      attgaggtg aacctaaat aacgagtcg tacacgaaa 
      ctgtaccaa tttctcgcc gatgtttta acaatccaa 
      cacaacgtt gcgattttt cacggaatt gagtcgcag 
      gaatacggg gctgcggaa cttatgatt agagactgc 
      atgcacgac ctgaccgag ttgaaactt tgcgttgat 
      tgttaccgc catagtaat gagaaaatt aacgctaag 
      tggttctgt ttgccgtgc agagtgcct cataagtta 
      gtttgggcc aaacaaaag ggctacccc tattggcct 
      gctaaagtg attcaagag acgagcacg cattttgat 
      gttaggttt tttgggggt aaatacgaa aggtcgctt 
      ctgatcaaa tcgttaatt aaaccgatt gaaacgccg 
      caatcgaag ttgcaaata aagacgtct tcggcgttt 
      aacaaggcg gtggaggag ttaaggttc catcagcgc 
      ttgatgcag aaccctgac gagctccag aagttgatc 
      agttcgtcg aaaaacagg gtcaggagt ttaaataag 
      cagcgaatt tcgacgttg aagaagtcg cttaatagt 
      tcactggaa gtgaaaaag agaaattca tttgaaatg 
      aatgatgtt tacaagttt gaggataat ccattcggt 
      cagcaaaat ttaagccta acgcataaa cgtaaaagc 
      agtttttgt gaaggaagt cccgtgtcg aaaaaaaat 
      tcaactagt tccgtggag aaaaacgag gaagttcac 
      aatacttcc gatagcgtt atacacata tcagacggc 
      gaagacgat gagtatccc tattacgat tattcatcg 
      gtttctaat acaaactat aatggttca tatgagcaa 
      gtttcaagt tccactgaa aattacaga ttccaggac 
      agaagccag gaaaatggg atgcagcaa cttgatcac 
      ccttatagt gattcagtt gaaaagatg agaagaaga 
      cttgaatgt gtgagtgat aaaaaagag cttataaag 
      tgtgcaatg gactgtatg caaagtgag attgatcaa 
      attacaaat aatcacaat gatcatttg aaaaggttg 
      tttgagtcc cacaatgcg cagatatcg gagattaag 
      aaaaagcaa tggtgctat aattgcgaa caggaagcc 
      atttatcat tgttgctgg aacacggcg tattgttcc 
      caaagttgt caacaacag cactggcaa gcagaacat 
      aaaaaagtt tgcaggcgt aagcgctag tttcataca 
      aaaattaaa aaatgttat tttgtgtat tatctattt 
      ttatagttt gtttatcct ctaatatca ttatgtaga 
      gtaatggga acattggcg taattttca ttgttttgg 
      tacttttta ggaccaagt tacgaaagt acaattcta 
      gttaacttt ttaattgta ggcaataac ttgagttgt 
      aagagttta tcacatagt gttgttgaa acttccaaa 
      tcattttta aacgttgta tcacaattg aaagttatg 
      ccactactt cattgtttg ttcttattt tatggttta 
      aaata