iBeetle: TC013862 iB

Gene information

General information
Tc number	TC013862	Gene The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.	NC_007420.3: 2761941..2765228
Sequence information
Transcripts / Proteins (1)	Get mRNA sequence Get CDS sequence Get protein sequence
Ortholog information (from OrthoDB) The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required. The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.
Closest fly homologs	Pof - Painting of fourth (CG3691)	View orthologs at OrthoDB for:	TC013862

Gene ontology

GO terms for Tribolium

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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

GO terms for the homologous Drosophila genes

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Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

Gene	{{ goGene }}	The Tribolium gene the GO term is assigned to.
GO ID	* {{ blurIdResult }}	The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
Evidence	{{ blurEvidenceResult }}	The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
Reference	* {{ blurReferenceResult }}	The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
Quotation		If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
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iBeetle screen iB_02213

Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

iB sequence

Phenotype after pupal injection

Usually 10 injected animals

Metamorphosis and survival

Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)

adult lethality - Number of animals showing the phenotype: 1 (9 dpi)

Analysis of embryonic musculature and early development

number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,

Analysis of larval stage 1 cuticle

number of eggs/embryos/larvae on slide: > 50

head potentially size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

Comment: All head appendages are present. Their shape looks irregular in a few cases. Especially when looking at the distance between the median and posterior vertex bristles the head size seems to be reduced.

head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

legs slightly shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%

Phenotype after larval injection

TR: 134924 potentially confused/crosscontaminated with : potentially confused/contaminated with, iB # (comma separated) 2214

iB_02214 is in the same well

Usually 10 injected animals

Metamorphosis and survival

Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)

potentially confused/crosscontaminated with: iB # (comma separated) 2214

both numbers in the same well but all pupae are wildtypic