General information | |||
TC013862 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 2761941..2765228 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC013862 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02213 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
head potentially size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Comment: All head appendages are present. Their shape looks irregular in a few cases. Especially when looking at the distance between the median and posterior vertex bristles the head size seems to be reduced. |
gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs slightly shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
iB_02214 is in the same well |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
potentially confused/crosscontaminated with: iB # (comma separated) 2214
both numbers in the same well but all pupae are wildtypic |
iB02214 in the same well |
animals of both injections in this well, but egg number is normal according to the number of animals |
animals of both injections in the same well, but all animals wildtypic |
both numbers in the same well but all pupae are wildtypic |
iB02214 in the same well |
animals of both injections in this well, but egg number is normal according to the number of animals |
iB02214 in the same well |
animals of both injections in this well, but egg number is normal according to the number of animals |
animals of both injections in the same well, but all animals wildtypic |
animals of both injections in this well, but egg number is normal according to the number of animals |
- >TC013862 RA
MSGNGYGYY YAASAQSFP VQNPNQSWS EWSLEQQQY NNSTRNAAP HPLNHVNNH TNAYVAPHR SQNHLYNAN NRHYQQRRY TNPNQTFPS QQQPWQRNN YKRPRPQDS PNDPEPKSK TGKKKKKPL SQNVPSKKD WTLEEAEMA LNVEKEYNK RYKNHSLII KFPDQELNR DIVSKFHPS IENVHFQQP STPRFCFVT LQESADAES VISALNKIK FGEGHLTAE YKKDREEDQ NIGPEDIDP CTLYVGNLA QEITKEDMV KTYPTSRRI DIGYAKKMK YTRYAFVSF KNVSESIDA FKKTHSTQM YSKSLIVRF RRLHGTVGM PGESKPQSP AKKTDSNNQ QEQTSERPE STTSCEVHE QNSTVVNDS LHSEVDLSV VKQEVNSSD DEITVNKTS KNYESPYMV GSIDVAFEQ HRPMIKKEI KRETEDDDL SFVQNSSGT QANGSIVQI KPEKENHPT VKRERDEDD ESSDDDYGG RISDVNFSS LLNQLEDHS NQIESLRKS VP
- >TC013862 RA
atgagtgga aatggttac ggttattat tacgccgca agtgcccaa agtttccca gtgcagaac cccaaccaa agttggagt gagtggtca ttggagcaa cagcaatac aacaacagc acgagaaat gccgcccca catccctta aatcacgtc aacaatcac acaaacgca tatgtggca ccacacagg tcccaaaac cacttatac aacgcaaac aacaggcac taccaacag agaaggtac acaaaccca aatcagaca tttcccagt caacaacag ccatggcaa agaaataat tacaaaaga ccgagacca caagattct ccaaatgac cccgaaccg aagagcaaa acgggcaaa aagaagaag aaaccgctg tcgcaaaac gtgccaagt aaaaaggac tggacgctg gaggaggca gaaatggca ctgaacgtg gaaaaagag tacaacaaa agatacaaa aaccactca cttattatc aaattcccc gatcaggag ttaaacagg gacatcgtc tccaaattc cacccaagc atcgaaaac gtgcatttc cagcagccc tcgaccccc agattctgc ttcgtcacc cttcaggaa tctgccgac gcagaatct gttataagt gctttaaac aagataaaa ttcggcgaa ggccattta accgctgag tacaagaag gaccgggaa gaggaccaa aacataggc cctgaagac atagaccct tgtactctg tacgttggc aaccttgct caagaaata acaaaggaa gatatggtc aaaacctat cccacaagc cggcggatc gacattggc tatgcgaaa aaaatgaaa tacacgcga tacgccttt gtctcgttt aaaaacgtg agtgagtca atcgacgcc ttcaagaaa acgcacagc acacaaatg tactccaaa agtctgatc gtgcgattt cgacgattg cacggaact gtgggaatg cctggggag tccaagccc cagagcccc gccaaaaaa acggactcg aacaaccaa caggaacaa accagtgaa agacctgaa agtacgacg agttgtgag gtgcatgag caaaacagc actgttgta aatgactcg ctacactct gaagttgat ttgagtgta gttaagcag gaagttaat agttccgat gatgaaatt acagtgaac aaaacgtcg aaaaattac gagtcgcct tacatggtc ggctcaatt gatgtggcg tttgaacag catagacca atgattaag aaagaaatt aagcgggaa actgaagat gatgacttg tcatttgta caaaattcg tcagggacg caagctaat ggctcaata gttcaaata aaaccagaa aaagaaaat cacccgacg gtgaaacgt gagcgtgac gaggacgat gagtcatcg gatgatgat tacggtggc aggataagc gatgtgaat tttagcagt ctgttaaac cagctagaa gaccattcc aatcagata gaatctctg agaaagtct gttccttaa
- >TC013862 RA
aaatctaaa caggttcaa ggttagata agtgtttca agctgtcaa aaattggta aataaataa attaaatat atgcccggt tggaggaat agccatgag tggaaatgg ttacggtta ttattacgc cgcaagtgc ccaaagttt cccagtgca gaaccccaa ccaaagttg gagtgagtg gtcattgga gcaacagca atacaacaa cagcacgag aaatgccgc cccacatcc cttaaatca cgtcaacaa tcacacaaa cgcatatgt ggcaccaca caggtccca aaaccactt atacaacgc aaacaacag gcactacca acagagaag gtacacaaa cccaaatca gacatttcc cagtcaaca acagccatg gcaaagaaa taattacaa aagaccgag accacaaga ttctccaaa tgaccccga accgaagag caaaacggg caaaaagaa gaagaaacc gctgtcgca aaacgtgcc aagtaaaaa ggactggac gctggagga ggcagaaat ggcactgaa cgtggaaaa agagtacaa caaaagata caaaaacca ctcacttat tatcaaatt ccccgatca ggagttaaa cagggacat cgtctccaa attccaccc aagcatcga aaacgtgca tttccagca gccctcgac ccccagatt ctgcttcgt cacccttca ggaatctgc cgacgcaga atctgttat aagtgcttt aaacaagat aaaattcgg cgaaggcca tttaaccgc tgagtacaa gaaggaccg ggaagagga ccaaaacat aggccctga agacataga cccttgtac tctgtacgt tggcaacct tgctcaaga aataacaaa ggaagatat ggtcaaaac ctatcccac aagccggcg gatcgacat tggctatgc gaaaaaaat gaaatacac gcgatacgc ctttgtctc gtttaaaaa cgtgagtga gtcaatcga cgccttcaa gaaaacgca cagcacaca aatgtactc caaaagtct gatcgtgcg atttcgacg attgcacgg aactgtggg aatgcctgg ggagtccaa gccccagag ccccgccaa aaaaacgga ctcgaacaa ccaacagga acaaaccag tgaaagacc tgaaagtac gacgagttg tgaggtgca tgagcaaaa cagcactgt tgtaaatga ctcgctaca ctctgaagt tgatttgag tgtagttaa gcaggaagt taatagttc cgatgatga aattacagt gaacaaaac gtcgaaaaa ttacgagtc gccttacat ggtcggctc aattgatgt ggcgtttga acagcatag accaatgat taagaaaga aattaagcg ggaaactga agatgatga cttgtcatt tgtacaaaa ttcgtcagg gacgcaagc taatggctc aatagttca aataaaacc agaaaaaga aaatcaccc gacggtgaa acgtgagcg tgacgagga cgatgagtc atcggatga tgattacgg tggcaggat aagcgatgt gaattttag cagtctgtt aaaccagct agaagacca ttccaatca gatagaatc tctgagaaa gtctgttcc ttaactttt atttattta accttacct ctatgtaat gtttatacg tattgcgtt gcggttaaa ccataagtt aataaaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
