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Gene information
General information
TC013862 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2761941..2765228
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013862
    iBeetle screen iB_02213

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head potentially size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: All head appendages are present. Their shape looks irregular in a few cases. Especially when looking at the distance between the median and posterior vertex bristles the head size seems to be reduced.
    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs slightly shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    TR: 134924 potentially confused/crosscontaminated with : potentially confused/contaminated with, iB # (comma separated) 2214
    iB_02214 is in the same well
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    both numbers in the same well but all pupae are wildtypic
    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    iB02214 in the same well
    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    animals of both injections in this well, but egg number is normal according to the number of animals
    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    animals of both injections in the same well, but all animals wildtypic
    Pupal morphology
    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    both numbers in the same well but all pupae are wildtypic
    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    iB02214 in the same well
    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    animals of both injections in this well, but egg number is normal according to the number of animals
    Adult morphology
    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    iB02214 in the same well
    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    animals of both injections in this well, but egg number is normal according to the number of animals
    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    animals of both injections in the same well, but all animals wildtypic
    Fertility
    potentially confused/crosscontaminated with: iB # (comma separated) 2214
    animals of both injections in this well, but egg number is normal according to the number of animals
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013862 RA
      MSGNGYGYY YAASAQSFP VQNPNQSWS EWSLEQQQY 
      NNSTRNAAP HPLNHVNNH TNAYVAPHR SQNHLYNAN 
      NRHYQQRRY TNPNQTFPS QQQPWQRNN YKRPRPQDS 
      PNDPEPKSK TGKKKKKPL SQNVPSKKD WTLEEAEMA 
      LNVEKEYNK RYKNHSLII KFPDQELNR DIVSKFHPS 
      IENVHFQQP STPRFCFVT LQESADAES VISALNKIK 
      FGEGHLTAE YKKDREEDQ NIGPEDIDP CTLYVGNLA 
      QEITKEDMV KTYPTSRRI DIGYAKKMK YTRYAFVSF 
      KNVSESIDA FKKTHSTQM YSKSLIVRF RRLHGTVGM 
      PGESKPQSP AKKTDSNNQ QEQTSERPE STTSCEVHE 
      QNSTVVNDS LHSEVDLSV VKQEVNSSD DEITVNKTS 
      KNYESPYMV GSIDVAFEQ HRPMIKKEI KRETEDDDL 
      SFVQNSSGT QANGSIVQI KPEKENHPT VKRERDEDD 
      ESSDDDYGG RISDVNFSS LLNQLEDHS NQIESLRKS 
      VP
    CDS sequence
    • >TC013862 RA
      atgagtgga aatggttac ggttattat tacgccgca 
      agtgcccaa agtttccca gtgcagaac cccaaccaa 
      agttggagt gagtggtca ttggagcaa cagcaatac 
      aacaacagc acgagaaat gccgcccca catccctta 
      aatcacgtc aacaatcac acaaacgca tatgtggca 
      ccacacagg tcccaaaac cacttatac aacgcaaac 
      aacaggcac taccaacag agaaggtac acaaaccca 
      aatcagaca tttcccagt caacaacag ccatggcaa 
      agaaataat tacaaaaga ccgagacca caagattct 
      ccaaatgac cccgaaccg aagagcaaa acgggcaaa 
      aagaagaag aaaccgctg tcgcaaaac gtgccaagt 
      aaaaaggac tggacgctg gaggaggca gaaatggca 
      ctgaacgtg gaaaaagag tacaacaaa agatacaaa 
      aaccactca cttattatc aaattcccc gatcaggag 
      ttaaacagg gacatcgtc tccaaattc cacccaagc 
      atcgaaaac gtgcatttc cagcagccc tcgaccccc 
      agattctgc ttcgtcacc cttcaggaa tctgccgac 
      gcagaatct gttataagt gctttaaac aagataaaa 
      ttcggcgaa ggccattta accgctgag tacaagaag 
      gaccgggaa gaggaccaa aacataggc cctgaagac 
      atagaccct tgtactctg tacgttggc aaccttgct 
      caagaaata acaaaggaa gatatggtc aaaacctat 
      cccacaagc cggcggatc gacattggc tatgcgaaa 
      aaaatgaaa tacacgcga tacgccttt gtctcgttt 
      aaaaacgtg agtgagtca atcgacgcc ttcaagaaa 
      acgcacagc acacaaatg tactccaaa agtctgatc 
      gtgcgattt cgacgattg cacggaact gtgggaatg 
      cctggggag tccaagccc cagagcccc gccaaaaaa 
      acggactcg aacaaccaa caggaacaa accagtgaa 
      agacctgaa agtacgacg agttgtgag gtgcatgag 
      caaaacagc actgttgta aatgactcg ctacactct 
      gaagttgat ttgagtgta gttaagcag gaagttaat 
      agttccgat gatgaaatt acagtgaac aaaacgtcg 
      aaaaattac gagtcgcct tacatggtc ggctcaatt 
      gatgtggcg tttgaacag catagacca atgattaag 
      aaagaaatt aagcgggaa actgaagat gatgacttg 
      tcatttgta caaaattcg tcagggacg caagctaat 
      ggctcaata gttcaaata aaaccagaa aaagaaaat 
      cacccgacg gtgaaacgt gagcgtgac gaggacgat 
      gagtcatcg gatgatgat tacggtggc aggataagc 
      gatgtgaat tttagcagt ctgttaaac cagctagaa 
      gaccattcc aatcagata gaatctctg agaaagtct 
      gttccttaa
    mRNA sequence
    • >TC013862 RA
      aaatctaaa caggttcaa ggttagata agtgtttca 
      agctgtcaa aaattggta aataaataa attaaatat 
      atgcccggt tggaggaat agccatgag tggaaatgg 
      ttacggtta ttattacgc cgcaagtgc ccaaagttt 
      cccagtgca gaaccccaa ccaaagttg gagtgagtg 
      gtcattgga gcaacagca atacaacaa cagcacgag 
      aaatgccgc cccacatcc cttaaatca cgtcaacaa 
      tcacacaaa cgcatatgt ggcaccaca caggtccca 
      aaaccactt atacaacgc aaacaacag gcactacca 
      acagagaag gtacacaaa cccaaatca gacatttcc 
      cagtcaaca acagccatg gcaaagaaa taattacaa 
      aagaccgag accacaaga ttctccaaa tgaccccga 
      accgaagag caaaacggg caaaaagaa gaagaaacc 
      gctgtcgca aaacgtgcc aagtaaaaa ggactggac 
      gctggagga ggcagaaat ggcactgaa cgtggaaaa 
      agagtacaa caaaagata caaaaacca ctcacttat 
      tatcaaatt ccccgatca ggagttaaa cagggacat 
      cgtctccaa attccaccc aagcatcga aaacgtgca 
      tttccagca gccctcgac ccccagatt ctgcttcgt 
      cacccttca ggaatctgc cgacgcaga atctgttat 
      aagtgcttt aaacaagat aaaattcgg cgaaggcca 
      tttaaccgc tgagtacaa gaaggaccg ggaagagga 
      ccaaaacat aggccctga agacataga cccttgtac 
      tctgtacgt tggcaacct tgctcaaga aataacaaa 
      ggaagatat ggtcaaaac ctatcccac aagccggcg 
      gatcgacat tggctatgc gaaaaaaat gaaatacac 
      gcgatacgc ctttgtctc gtttaaaaa cgtgagtga 
      gtcaatcga cgccttcaa gaaaacgca cagcacaca 
      aatgtactc caaaagtct gatcgtgcg atttcgacg 
      attgcacgg aactgtggg aatgcctgg ggagtccaa 
      gccccagag ccccgccaa aaaaacgga ctcgaacaa 
      ccaacagga acaaaccag tgaaagacc tgaaagtac 
      gacgagttg tgaggtgca tgagcaaaa cagcactgt 
      tgtaaatga ctcgctaca ctctgaagt tgatttgag 
      tgtagttaa gcaggaagt taatagttc cgatgatga 
      aattacagt gaacaaaac gtcgaaaaa ttacgagtc 
      gccttacat ggtcggctc aattgatgt ggcgtttga 
      acagcatag accaatgat taagaaaga aattaagcg 
      ggaaactga agatgatga cttgtcatt tgtacaaaa 
      ttcgtcagg gacgcaagc taatggctc aatagttca 
      aataaaacc agaaaaaga aaatcaccc gacggtgaa 
      acgtgagcg tgacgagga cgatgagtc atcggatga 
      tgattacgg tggcaggat aagcgatgt gaattttag 
      cagtctgtt aaaccagct agaagacca ttccaatca 
      gatagaatc tctgagaaa gtctgttcc ttaactttt 
      atttattta accttacct ctatgtaat gtttatacg 
      tattgcgtt gcggttaaa ccataagtt aataaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY