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Gene information
General information
TC013805 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 2736832..2745651
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013805
    iBeetle screen iB_02210
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    about 55

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013805 RA
      MTILTKPIS GEKKDKLAP LVQNDQLAS APPTEDVEG 
      QPSDVVFLK ARARRVSTA TTLCLILTS LIVVSIGIF 
      VGKSLYNQY ISAQMRRFT GYAQIPMPS EDVETLYFR 
      ERNPMPDSA LLKALAGDD DDDDDNNNV KDDFDNIMR 
      NYFREDFEI DLDGEKYEK IDVPDFRDG RSGRFIHDF 
      NTNTTGIID ITGNRCFVM PLNRGHVLP PRSLFDLVN 
      KMWDGYYKV DTQVVRETM RVVTPPLKD LKGVGSYIA 
      KECENRPVY KLEKYVGGV VKRSADLHV QAKFAQFSG 
      NKITEFDIL NFEDVQDYE KKNNLH
    CDS sequence
    • >TC013805 RA
      atgaccatc ctgactaag cccatttcc ggggaaaag 
      aaggacaag ctagcgccc cttgtccag aatgaccag 
      ttggcatca gcgccgcca accgaagac gtggagggc 
      cagccctcc gacgtggtg ttcctcaaa gctcgcgct 
      cgtcgcgtc tccaccgcc acaaccctc tgcctaatt 
      ctcacttca ctcatcgtc gtcagtatc ggcattttc 
      gtcggtaaa tccctctac aaccaatac atcagcgct 
      caaatgcgt cgtttcacc ggctacgct caaatcccg 
      atgccttcg gaggacgtc gaaacactg tatttccgc 
      gaacgcaac ccaatgccc gattcggcc ctccttaag 
      gccctcgct ggcgatgac gacgacgac gatgacaat 
      aacaacgtc aaagacgat ttcgacaac atcatgagg 
      aactacttc cgagaggat ttcgagatc gacctcgat 
      ggggagaag tacgaaaaa atcgacgtt cctgatttc 
      agggatgga cgaagcggg agatttatt cacgatttt 
      aatacaaac acgacgggg attattgat attacgggg 
      aatcgatgc tttgtgatg ccgctgaat cgagggcat 
      gttttgccg ccgaggtcg ctttttgat ttggttaat 
      aaaatgtgg gatgggtat tataaagtt gatacgcaa 
      gtcgtgagg gagactatg agagtggtg acgccaccg 
      ttgaaggat ttgaaggga gttggcagt tatattgcg 
      aaggagtgt gaaaataga cccgtttac aagcttgaa 
      aaatacgtc ggaggagtg gttaaacga tcagccgat 
      ttgcatgtg caagccaag tttgctcag ttctctggc 
      aataaaata acagagttt gatattttg aattttgag 
      gatgttcaa gattatgag aagaagaac aatcttcat 
      taa
    mRNA sequence
    • >TC013805 RA
      tgttttggc gaagtgaaa agcgaatcg atcaattac 
      gcattttct gaaaattcg ggtgtaaat tttccgtcg 
      taatgacca tcctgacta agcccattt ccggggaaa 
      agaaggaca agctagcgc cccttgtcc agaatgacc 
      agttggcat cagcgccgc caaccgaag acgtggagg 
      gccagccct ccgacgtgg tgttcctca aagctcgcg 
      ctcgtcgcg tctccaccg ccacaaccc tctgcctaa 
      ttctcactt cactcatcg tcgtcagta tcggcattt 
      tcgtcggta aatccctct acaaccaat acatcagcg 
      ctcaaatgc gtcgtttca ccggctacg ctcaaatcc 
      cgatgcctt cggaggacg tcgaaacac tgtatttcc 
      gcgaacgca acccaatgc ccgattcgg ccctcctta 
      aggccctcg ctggcgatg acgacgacg acgatgaca 
      ataacaacg tcaaagacg atttcgaca acatcatga 
      ggaactact tccgagagg atttcgaga tcgacctcg 
      atggggaga agtacgaaa aaatcgacg ttcctgatt 
      tcagggatg gacgaagcg ggagattta ttcacgatt 
      ttaatacaa acacgacgg ggattattg atattacgg 
      ggaatcgat gctttgtga tgccgctga atcgagggc 
      atgttttgc cgccgaggt cgctttttg atttggtta 
      ataaaatgt gggatgggt attataaag ttgatacgc 
      aagtcgtga gggagacta tgagagtgg tgacgccac 
      cgttgaagg atttgaagg gagttggca gttatattg 
      cgaaggagt gtgaaaata gacccgttt acaagcttg 
      aaaaatacg tcggaggag tggttaaac gatcagccg 
      atttgcatg tgcaagcca agtttgctc agttctctg 
      gcaataaaa taacagagt ttgatattt tgaattttg 
      aggatgttc aagattatg agaagaaga acaatcttc 
      attaagtgt tgtaagagc tagagagat tttttcatt 
      atatttgct tttctatga tattcgatt ttgagacgt 
      aatgcaggt cgaaatatg tataaattt gcgtaattt 
      ttctgtttt tatgtagat gggatcggt ccgaggacc 
      ggttgtata ataataaaa aaagtttaa aggacgtta 
      ctgtgcatg taattgttg tacaatttt atatacaat 
      taaattatc cgattgtac ttacatttt atgtgttag 
      gtacacgtt ttcctgtgc aatatgtta ctttaccga 
      catatcacg tttgtcttg aataaataa aacaacaac 
      agttagtcc gaaaataat ttttattta cattt