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Gene information
General information
TC013803 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3799189..3808589
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013803
    iBeetle screen iB_02209

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late partiallyrounded - Number of animals showing the phenotype: 4
    vitellogenic egg chamber latenumber decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber partiallyfused - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    elytra blistered - Number of animals showing the phenotype: 7

    hindwing partially blistered - Number of animals showing the phenotype: 1

    longitudinal dorsal thoracic muscles partially not present - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013803 RA
      MLETRSVRG MPEPSWQSK SNFGAKSTP HAMAAPNIR 
      HGKTGQDDI CHVVAKYDY AAQGPQELD LRKNERYLL 
      LDDSKHWWK VQNSRNQAG YVPSNYVKK EKPSLFDSI 
      KKKVKKGAG SKTLPSNNS PSRTVESPS MARRQAADP 
      SEAIGTAVV KYNYQAQQP DELSLVKGS RILILEKSN 
      DGWWRGQSG NVAGWFPSN YTQEEGDAD DTLHTYAMA 
      ENVLDIVVA LYSFSSTND QELSFEKGD RLEILDRPP 
      SDPEWYKAR NAQGQVGLV PRNYLQELS EYLTQPFLD 
      RKQEPMERS QVAMGDKPH LVDKPWYYG SITRYMCDN 
      LLNQRGHDG DFLIRDSET NVGDYSVSL KAPGRNKHF 
      RVHVEGALY CIGQRKFHT LDQLVDHYQ RAPIYTNKQ 
      GEKLYLVRP LPKPTTNG
    CDS sequence
    • >TC013803 RA
      atgttggag accagatct gttcgggga atgccagag 
      ccttcttgg cagtctaag agtaatttc ggcgctaaa 
      tcgaccccc catgcgatg gctgcacca aacatacga 
      catggcaaa actgggcaa gatgacatt tgccatgtt 
      gtggccaag tacgattac gccgcacag ggcccccag 
      gagctcgac ctcaggaag aacgagcgc tatttgctc 
      ctcgacgac tccaagcat tggtggaaa gtgcagaat 
      tcgagaaat caagccggt tatgtgcca agcaactat 
      gttaagaaa gagaaaccg tcacttttt gatagtatc 
      aagaaaaag gtaaaaaag ggcgccggc tctaaaacc 
      ctcccttcg aataactct ccctcccgg acggtggaa 
      tcccccagt atggcgcgt cgtcaagcc gccgatccc 
      tccgaagcc atcggcacc gccgtcgtc aagtacaat 
      tatcaagcc caacaaccc gacgaactt tccctcgtc 
      aaaggttcc cgaattctc atcttggag aagagcaac 
      gacggctgg tggcgaggt cagagcggg aacgtcgcc 
      ggctggttc ccttcgaac tacacccag gaggagggc 
      gacgccgac gataccctt cacacgtac gcgatggca 
      gaaaacgtg ctagatatc gtcgtggcg ttgtattcg 
      ttctcgtcg acaaacgac caggagttg tcttttgaa 
      aagggcgac cgattggag attttagat cggcctccg 
      agtgatccc gaatggtac aaagcgagg aatgcgcaa 
      ggacaggtc ggcttggtg ccgaggaat tacctgcag 
      gagttgtcc gagtatttg actcagccg tttctcgac 
      cgaaaacag gagccgatg gagaggtcg caagtggca 
      atgggcgat aaaccccat ctggtggac aagccgtgg 
      tattatggg agtatcacg aggtatatg tgtgataat 
      ctcctgaat cagagagga cacgatggg gattttctc 
      atcagggat agtgaaacg aatgtgggg gattattcg 
      gtgtcgctg aaggccccc ggacgcaac aagcacttc 
      cgcgtccac gtcgagggg gcgctgtac tgcatcgga 
      caacgaaaa ttccacacg ttagaccaa ctcgtcgac 
      cactaccag agggcgccg atttacacg aacaaacag 
      ggcgaaaaa ttgtacctc gtacgtcca ttacctaag 
      ccaaccacc aacggataa
    mRNA sequence
    • >TC013803 RA
      aagctcccc tgtgcgatt gtgatgtct tctgaaaat 
      accgtggag ggtgggagc aataatttt ctatgtgtg 
      aaactgttg caaatggtg cttcaaccc gaccactga 
      tgtgtaata aagtactat gtccagttt gaaaatcgt 
      ggtagaagc atgaatgtg ggctagcac tagatagtt 
      gtgcttgtg gtcatgttg gagaccaga tctgttcgg 
      ggaatgcca gagccttct tggcagtct aagagtaat 
      ttcggcgct aaatcgacc ccccatgcg atggctgca 
      ccaaacata cgacatggc aaaactggg caagatgac 
      atttgccat gttgtggcc aagtacgat tacgccgca 
      cagggcccc caggagctc gacctcagg aagaacgag 
      cgctatttg ctcctcgac gactccaag cattggtgg 
      aaagtgcag aattcgaga aatcaagcc ggttatgtg 
      ccaagcaac tatgttaag aaagagaaa ccgtcactt 
      tttgatagt atcaagaaa aaggtaaaa aagggcgcc 
      ggctctaaa accctccct tcgaataac tctccctcc 
      cggacggtg gaatccccc agtatggcg cgtcgtcaa 
      gccgccgat ccctccgaa gccatcggc accgccgtc 
      gtcaagtac aattatcaa gcccaacaa cccgacgaa 
      ctttccctc gtcaaaggt tcccgaatt ctcatcttg 
      gagaagagc aacgacggc tggtggcga ggtcagagc 
      gggaacgtc gccggctgg ttcccttcg aactacacc 
      caggaggag ggcgacgcc gacgatacc cttcacacg 
      tacgcgatg gcagaaaac gtgctagat atcgtcgtg 
      gcgttgtat tcgttctcg tcgacaaac gaccaggag 
      ttgtctttt gaaaagggc gaccgattg gagatttta 
      gatcggcct ccgagtgat cccgaatgg tacaaagcg 
      aggaatgcg caaggacag gtcggcttg gtgccgagg 
      aattacctg caggagttg tccgagtat ttgactcag 
      ccgtttctc gaccgaaaa caggagccg atggagagg 
      tcgcaagtg gcaatgggc gataaaccc catctggtg 
      gacaagccg tggtattat gggagtatc acgaggtat 
      atgtgtgat aatctcctg aatcagaga ggacacgat 
      ggggatttt ctcatcagg gatagtgaa acgaatgtg 
      ggggattat tcggtgtcg ctgaaggcc cccggacgc 
      aacaagcac ttccgcgtc cacgtcgag ggggcgctg 
      tactgcatc ggacaacga aaattccac acgttagac 
      caactcgtc gaccactac cagagggcg ccgatttac 
      acgaacaaa cagggcgaa aaattgtac ctcgtacgt 
      ccattacct aagccaacc accaacgga taaagacag 
      ttttgattc tctcttttt ttgtgtgtc acaagtgac 
      gttaaaaac gaaaaatct ggtcatttt tatgtatat 
      ttttttatt tcgtgagcc aagagggcg cgtgtatta 
      tgtcaaata ccagtagtc acaagcaca actagttct 
      cagtgttga agaagaaga aaacattgc agcttttat 
      atcgtcaat actcataac gtaggtgta aggattggt 
      tgcttccgg aaatttcga tgatataaa gccaaaatg 
      gatagttaa ggacggaat tatattttt tgtaaacga 
      gttttgatt gactagtgg tacagtctt attttccac 
      tgtatttat tatacataa acgtaatta taggtaatt 
      gatgtgaga gtttttgtg agtttgaat tatttaata 
      ataaatata tggtctcat cttctgatt atttacagg 
      aacacaccc gttcgaatt attgttaaa aatcaaatt 
      aatcgactg ttgaaatta ttacaaagt tggagagtg 
      taaaagttt ttctacttt ttataatta aaattgtaa 
      aaaaatcat atcataaat caaataact gcgcttaga 
      tagcttgac attttttga gtaccttat tgtaatttt 
      tttataacg aaggctctt aggaccatt tatttactc 
      gatatgacg taattagga taattttaa tcctggagt 
      tggacactc catactcgg ccacattag acggttagt 
      gaataaata gtttaagct ttgtgacaa cttctgtca 
      ttaaaataa ttgtgtgta ttatgtaaa taaacatga 
      gcctgtatg tcaaacgag tattgcata tttctcaat 
      atacttata caaaccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY