| General information | |||
| TC013799 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 3832893..3868699 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013799 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02208 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 44.44% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)
- >TC013799 RA
MTSKGSKCR VLLHQCLAI IVRKEGCRG DPGDFWMYE NGYLLFQGL LSSNSLCWW NTGLNGATK TLTYHGYIS PGAILVGSE PCAIDIVKN AWARRVLQP PPGYQIVAL EDIECDGSP VAQTQWTPL PEAVCAVIL RLSRQGRPA GIETIREAL IMAFPHVSP PSEQALYDT LVQLTTERK LYHTSNGYF IVTPERRRS RSASRGRKR VEDHDHEPL SGKTMLMST EEALVMVHG DMATIRDGD VTHQLIQTN LADVICGGN SSDKILYPR STKHRASSL SVPRSPDRR GSFRLWRSS RRLRRSAST RTIAKHYVD NTSSSTENS APSTPSPKK ESLLTRLFK KTKRSQCQI SSFSAQFPP TEWFNSKAV HLHSIGTQT STTDNEVAS ITSYLENCE IKPTRSATL PRRRCRQYP DLPYPPYPL SRENSPVTR KNPYMPCTL PRNKQKSRD SSRKTSRSS SRQTEEKPE SVKTSQKLE VPDNSGPSS IESHKSSTK TESSFNSGP SSLESHKTV INTKEKAKK TTNNRTCIP QSNSFTLQV TTNQGGKLN NKPNATTTT TINGEGGSS TKIFVQNSP VRSVITFEN GQCDDRNPN LVIINDTEP REQIPTKNT NQSKETTLN NSRDLLPNG ESLEQKNNS RKLSLQLPN NLIKNVSTH KFVSNSNPD SPIIGHEVP KGGYNSTPP SPTKSTDAL SMSSIGNIY TKNEMGSLA SFDSCKKLE KNMLGSEPN ISFKDNRDQ QFPSLNDLS FNFTSLAAQ KILKGVGLN SVDTLVELN MTANLDKQN NCDVVHTDF GMF
- >TC013799 RA
atgacctcg aagggctcc aagtgcaga gtcctcttg caccagtgc ctggccatc atcgtcagg aaggagggc tgtcggggc gacccgggc gacttctgg atgtacgaa aatggatat ctgctcttc cagggtttg ctatcatcg aacagcctt tgctggtgg aacaccggt ctaaacgga gccacgaag acgctaacc tatcacggc tacatcagc cccggagcc atcctcgtc ggttctgaa ccctgtgct atcgacatc gtcaaaaac gcatgggct agacgcgtc ctccagcct cctccaggc taccagatc gtggctttg gaggatatc gagtgtgat ggtagtccg gttgcccag acccaatgg acgccgctt ccggaggcc gtttgtgcc gttatcctc cggctgtcg cgccaaggg cggcctgct gggatcgag accatcagg gaggccctc atcatggcc ttcccccac gtctcgccc ccaagtgag caggccttg tacgacact ctggtgcaa ctgaccacc gagaggaag ttgtaccat acctccaat gggtacttt atcgtcact ccagaacgg aggaggtcg cggagcgcg tcacgtggc cggaagcga gtggaggat cacgaccac gaaccactt tccggtaaa acgatgctc atgagtaca gaagaagcg cttgttatg gttcatgga gacatggcc actatacga gacggtgat gttacgcac caactcatc caaaccaac ttggccgac gttatatgt ggagggaac tcgagcgat aagattctc tacccgaga agtaccaaa cacagggcc tcctcctta tcagtacca cgaagtccg gaccggagg ggctccttc aggctgtgg aggtccagc cggaggctg aggaggtcg gcctccacg aggaccata gccaaacac tacgtggac aacacgagt agtagtacc gaaaattca gcgccttca acgccaagt ccgaaaaaa gagtcgctt ttgacgcgc ctcttcaag aaaaccaaa cgctcccag tgccaaatc agctcattc agtgctcaa ttcccgcca actgagtgg ttcaactcc aaagccgtc catttacac agcatcggg acccaaacc tcgaccaca gataatgag gttgcctcc atcacatct tatttggaa aactgcgag atcaaacca acacgttcg gccaccctc ccccgccgc cgctgccgc caatacccc gacctaccc taccccccg tacccccta tcccgtgaa aattccccc gtcacccgc aaaaacccc tacatgcct tgcaccctc ccgcgcaac aagcagaaa agccgcgac tcctcccgg aagacaagc cggagcagt tcccggcaa accgaggag aaacccgaa agtgtgaaa actagtcaa aaactggaa gttcccgac aacagcgga ccctcgagc atcgagtcg cataagagc agcaccaag acggagagc tcgttcaac agcgggccc tccagtctc gagtcgcac aaaaccgtc atcaacacc aaggagaag gccaaaaag acaaccaac aaccggacc tgcatcccg caaagcaac tcgttcacg ctgcaagtg acgaccaat cagggcgga aagctgaac aacaagccc aacgccacc accaccacg accatcaac ggggaaggc ggcagcagt acgaagatc ttcgtgcag aattcgccg gttcgctcg gttataacc ttcgagaac gggcagtgc gacgaccgc aaccccaac ctggtcata atcaacgac acggagccg cgggagcaa atcccgacg aaaaacacg aaccagtcc aaagaaacg actctgaac aactcgaga gacctgctc ccgaacggc gagagcctc gagcagaag aacaacagt cgcaaatta tcgcttcaa cttcccaat aaccttata aaaaacgtc tcgacgcac aagttcgtc tccaattcc aacccagac tcgcccatc atcgggcac gaagtgcct aaagggggc tacaacagc acccctcct agtcccacc aagagcacc gacgccctg tcgatgtcc tccattggg aacatttac accaagaac gaaatgggt tcgctggcg agtttcgat tcgtgcaaa aagctcgag aaaaatatg ctgggttct gagcccaat atcagtttt aaggacaat cgcgaccaa caatttccc agtcttaat gacctttcg tttaatttt acgagtttg gcggcacag aaaatcctc aagggagtg ggtctaaat agtgtcgat acgttggtt gagctgaat atgacggcc aatttggat aaacagaac aactgtgat gtggttcat acggatttt gggatgttt tag
- >TC013799 RA
tgtaacaat ttaaagagg caataaaaa tgcctcagt tcaaaattt atcgaatgt taaaccatc aatttatca tttaattgt gtcaaattg gagttaaaa gcgcccatt tatctattt gaaaacttt cgaaaatct gtcaatctt gatacaaat ctatcgaat tagtcgagt tgatttctt tcacccacg acctcatcc attgttgga tggcatctg tggtcccaa cccaggaac tgctcttta gctatcact ttcatcatc ccctttttc ctcataggt cacccatcg ccttcgtcg aaaggtcat tgacgcggt gcggtgcgt aaaaatcgc aaaatgata accggtgtc atgcaaggt tagtgctcg ttgcccata ataggccta aacgaggct gcatcatgt ccaaaagtt gcccaaaaa acctcccat tcaacaacc accggagca acaaatagc caaaccggt tacctgctg catagcatt ctctcaaaa attatacag attccaatg agcgctggc tacgcaagt ctccgcgac taccacacc gatttcttt agtcttggt tctactttc aaggatgta acaggtgta caactctgt gctagatct gtttgcagt gtttgggtt caagtgatc gattgctaa aatgattgc tagttcgtg aaacatgac ctcgaaggg ctccaagtg cagagtcct cttgcacca gtgcctggc catcatcgt caggaagga gggctgtcg gggcgaccc gggcgactt ctggatgta cgaaaatgg atatctgct cttccaggg tttgctatc atcgaacag cctttgctg gtggaacac cggtctaaa cggagccac gaagacgct aacctatca cggctacat cagccccgg agccatcct cgtcggttc tgaaccctg tgctatcga catcgtcaa aaacgcatg ggctagacg cgtcctcca gcctcctcc aggctacca gatcgtggc tttggagga tatcgagtg tgatggtag tccggttgc ccagaccca atggacgcc gcttccgga ggccgtttg tgccgttat cctccggct gtcgcgcca agggcggcc tgctgggat cgagaccat cagggaggc cctcatcat ggccttccc ccacgtctc gcccccaag tgagcaggc cttgtacga cactctggt gcaactgac caccgagag gaagttgta ccatacctc caatgggta ctttatcgt cactccaga acggaggag gtcgcggag cgcgtcacg tggccggaa gcgagtgga ggatcacga ccacgaacc actttccgg taaaacgat gctcatgag tacagaaga agcgcttgt tatggttca tggagacat ggccactat acgagacgg tgatgttac gcaccaact catccaaac caacttggc cgacgttat atgtggagg gaactcgag cgataagat tctctaccc gagaagtac caaacacag ggcctcctc cttatcagt accacgaag tccggaccg gaggggctc cttcaggct gtggaggtc cagccggag gctgaggag gtcggcctc cacgaggac catagccaa acactacgt ggacaacac gagtagtag taccgaaaa ttcagcgcc ttcaacgcc aagtccgaa aaaagagtc gcttttgac gcgcctctt caagaaaac caaacgctc ccagtgcca aatcagctc attcagtgc tcaattccc gccaactga gtggttcaa ctccaaagc cgtccattt acacagcat cgggaccca aacctcgac cacagataa tgaggttgc ctccatcac atcttattt ggaaaactg cgagatcaa accaacacg ttcggccac cctcccccg ccgccgctg ccgccaata ccccgacct accctaccc cccgtaccc cctatcccg tgaaaattc ccccgtcac ccgcaaaaa cccctacat gccttgcac cctcccgcg caacaagca gaaaagccg cgactcctc ccggaagac aagccggag cagttcccg gcaaaccga ggagaaacc cgaaagtgt gaaaactag tcaaaaact ggaagttcc cgacaacag cggaccctc gagcatcga gtcgcataa gagcagcac caagacgga gagctcgtt caacagcgg gccctccag tctcgagtc gcacaaaac cgtcatcaa caccaagga gaaggccaa aaagacaac caacaaccg gacctgcat cccgcaaag caactcgtt cacgctgca agtgacgac caatcaggg cggaaagct gaacaacaa gcccaacgc caccaccac cacgaccat caacgggga aggcggcag cagtacgaa gatcttcgt gcagaattc gccggttcg ctcggttat aaccttcga gaacgggca gtgcgacga ccgcaaccc caacctggt cataatcaa cgacacgga gccgcggga gcaaatccc gacgaaaaa cacgaacca gtccaaaga aacgactct gaacaactc gagagacct gctcccgaa cggcgagag cctcgagca gaagaacaa cagtcgcaa attatcgct tcaacttcc caataacct tataaaaaa cgtctcgac gcacaagtt cgtctccaa ttccaaccc agactcgcc catcatcgg gcacgaagt gcctaaagg gggctacaa cagcacccc tcctagtcc caccaagag caccgacgc cctgtcgat gtcctccat tgggaacat ttacaccaa gaacgaaat gggttcgct ggcgagttt cgattcgtg caaaaagct cgagaaaaa tatgctggg ttctgagcc caatatcag ttttaagga caatcgcga ccaacaatt tcccagtct taatgacct ttcgtttaa ttttacgag tttggcggc acagaaaat cctcaaggg agtgggtct aaatagtgt cgatacgtt ggttgagct gaatatgac ggccaattt ggataaaca gaacaactg tgatgtggt tcatacgga ttttgggat gttttagat ggtcttttt gccggcttt ggacaaagt cggttttaa aggattgta acatatgta tttttagcg agttaggtc agtcaccgg ctgaacatg tatttaata ttttatata cgattttat gtaattatg cagaataaa ctattatat ttaca
