Status
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Gene information
General information
TC013799 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3832893..3868699
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013799
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02208

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 44.44% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013799 RA
      MTSKGSKCR VLLHQCLAI IVRKEGCRG DPGDFWMYE 
      NGYLLFQGL LSSNSLCWW NTGLNGATK TLTYHGYIS 
      PGAILVGSE PCAIDIVKN AWARRVLQP PPGYQIVAL 
      EDIECDGSP VAQTQWTPL PEAVCAVIL RLSRQGRPA 
      GIETIREAL IMAFPHVSP PSEQALYDT LVQLTTERK 
      LYHTSNGYF IVTPERRRS RSASRGRKR VEDHDHEPL 
      SGKTMLMST EEALVMVHG DMATIRDGD VTHQLIQTN 
      LADVICGGN SSDKILYPR STKHRASSL SVPRSPDRR 
      GSFRLWRSS RRLRRSAST RTIAKHYVD NTSSSTENS 
      APSTPSPKK ESLLTRLFK KTKRSQCQI SSFSAQFPP 
      TEWFNSKAV HLHSIGTQT STTDNEVAS ITSYLENCE 
      IKPTRSATL PRRRCRQYP DLPYPPYPL SRENSPVTR 
      KNPYMPCTL PRNKQKSRD SSRKTSRSS SRQTEEKPE 
      SVKTSQKLE VPDNSGPSS IESHKSSTK TESSFNSGP 
      SSLESHKTV INTKEKAKK TTNNRTCIP QSNSFTLQV 
      TTNQGGKLN NKPNATTTT TINGEGGSS TKIFVQNSP 
      VRSVITFEN GQCDDRNPN LVIINDTEP REQIPTKNT 
      NQSKETTLN NSRDLLPNG ESLEQKNNS RKLSLQLPN 
      NLIKNVSTH KFVSNSNPD SPIIGHEVP KGGYNSTPP 
      SPTKSTDAL SMSSIGNIY TKNEMGSLA SFDSCKKLE 
      KNMLGSEPN ISFKDNRDQ QFPSLNDLS FNFTSLAAQ 
      KILKGVGLN SVDTLVELN MTANLDKQN NCDVVHTDF 
      GMF
    CDS sequence
    • >TC013799 RA
      atgacctcg aagggctcc aagtgcaga gtcctcttg 
      caccagtgc ctggccatc atcgtcagg aaggagggc 
      tgtcggggc gacccgggc gacttctgg atgtacgaa 
      aatggatat ctgctcttc cagggtttg ctatcatcg 
      aacagcctt tgctggtgg aacaccggt ctaaacgga 
      gccacgaag acgctaacc tatcacggc tacatcagc 
      cccggagcc atcctcgtc ggttctgaa ccctgtgct 
      atcgacatc gtcaaaaac gcatgggct agacgcgtc 
      ctccagcct cctccaggc taccagatc gtggctttg 
      gaggatatc gagtgtgat ggtagtccg gttgcccag 
      acccaatgg acgccgctt ccggaggcc gtttgtgcc 
      gttatcctc cggctgtcg cgccaaggg cggcctgct 
      gggatcgag accatcagg gaggccctc atcatggcc 
      ttcccccac gtctcgccc ccaagtgag caggccttg 
      tacgacact ctggtgcaa ctgaccacc gagaggaag 
      ttgtaccat acctccaat gggtacttt atcgtcact 
      ccagaacgg aggaggtcg cggagcgcg tcacgtggc 
      cggaagcga gtggaggat cacgaccac gaaccactt 
      tccggtaaa acgatgctc atgagtaca gaagaagcg 
      cttgttatg gttcatgga gacatggcc actatacga 
      gacggtgat gttacgcac caactcatc caaaccaac 
      ttggccgac gttatatgt ggagggaac tcgagcgat 
      aagattctc tacccgaga agtaccaaa cacagggcc 
      tcctcctta tcagtacca cgaagtccg gaccggagg 
      ggctccttc aggctgtgg aggtccagc cggaggctg 
      aggaggtcg gcctccacg aggaccata gccaaacac 
      tacgtggac aacacgagt agtagtacc gaaaattca 
      gcgccttca acgccaagt ccgaaaaaa gagtcgctt 
      ttgacgcgc ctcttcaag aaaaccaaa cgctcccag 
      tgccaaatc agctcattc agtgctcaa ttcccgcca 
      actgagtgg ttcaactcc aaagccgtc catttacac 
      agcatcggg acccaaacc tcgaccaca gataatgag 
      gttgcctcc atcacatct tatttggaa aactgcgag 
      atcaaacca acacgttcg gccaccctc ccccgccgc 
      cgctgccgc caatacccc gacctaccc taccccccg 
      tacccccta tcccgtgaa aattccccc gtcacccgc 
      aaaaacccc tacatgcct tgcaccctc ccgcgcaac 
      aagcagaaa agccgcgac tcctcccgg aagacaagc 
      cggagcagt tcccggcaa accgaggag aaacccgaa 
      agtgtgaaa actagtcaa aaactggaa gttcccgac 
      aacagcgga ccctcgagc atcgagtcg cataagagc 
      agcaccaag acggagagc tcgttcaac agcgggccc 
      tccagtctc gagtcgcac aaaaccgtc atcaacacc 
      aaggagaag gccaaaaag acaaccaac aaccggacc 
      tgcatcccg caaagcaac tcgttcacg ctgcaagtg 
      acgaccaat cagggcgga aagctgaac aacaagccc 
      aacgccacc accaccacg accatcaac ggggaaggc 
      ggcagcagt acgaagatc ttcgtgcag aattcgccg 
      gttcgctcg gttataacc ttcgagaac gggcagtgc 
      gacgaccgc aaccccaac ctggtcata atcaacgac 
      acggagccg cgggagcaa atcccgacg aaaaacacg 
      aaccagtcc aaagaaacg actctgaac aactcgaga 
      gacctgctc ccgaacggc gagagcctc gagcagaag 
      aacaacagt cgcaaatta tcgcttcaa cttcccaat 
      aaccttata aaaaacgtc tcgacgcac aagttcgtc 
      tccaattcc aacccagac tcgcccatc atcgggcac 
      gaagtgcct aaagggggc tacaacagc acccctcct 
      agtcccacc aagagcacc gacgccctg tcgatgtcc 
      tccattggg aacatttac accaagaac gaaatgggt 
      tcgctggcg agtttcgat tcgtgcaaa aagctcgag 
      aaaaatatg ctgggttct gagcccaat atcagtttt 
      aaggacaat cgcgaccaa caatttccc agtcttaat 
      gacctttcg tttaatttt acgagtttg gcggcacag 
      aaaatcctc aagggagtg ggtctaaat agtgtcgat 
      acgttggtt gagctgaat atgacggcc aatttggat 
      aaacagaac aactgtgat gtggttcat acggatttt 
      gggatgttt tag
    mRNA sequence
    • >TC013799 RA
      tgtaacaat ttaaagagg caataaaaa tgcctcagt 
      tcaaaattt atcgaatgt taaaccatc aatttatca 
      tttaattgt gtcaaattg gagttaaaa gcgcccatt 
      tatctattt gaaaacttt cgaaaatct gtcaatctt 
      gatacaaat ctatcgaat tagtcgagt tgatttctt 
      tcacccacg acctcatcc attgttgga tggcatctg 
      tggtcccaa cccaggaac tgctcttta gctatcact 
      ttcatcatc ccctttttc ctcataggt cacccatcg 
      ccttcgtcg aaaggtcat tgacgcggt gcggtgcgt 
      aaaaatcgc aaaatgata accggtgtc atgcaaggt 
      tagtgctcg ttgcccata ataggccta aacgaggct 
      gcatcatgt ccaaaagtt gcccaaaaa acctcccat 
      tcaacaacc accggagca acaaatagc caaaccggt 
      tacctgctg catagcatt ctctcaaaa attatacag 
      attccaatg agcgctggc tacgcaagt ctccgcgac 
      taccacacc gatttcttt agtcttggt tctactttc 
      aaggatgta acaggtgta caactctgt gctagatct 
      gtttgcagt gtttgggtt caagtgatc gattgctaa 
      aatgattgc tagttcgtg aaacatgac ctcgaaggg 
      ctccaagtg cagagtcct cttgcacca gtgcctggc 
      catcatcgt caggaagga gggctgtcg gggcgaccc 
      gggcgactt ctggatgta cgaaaatgg atatctgct 
      cttccaggg tttgctatc atcgaacag cctttgctg 
      gtggaacac cggtctaaa cggagccac gaagacgct 
      aacctatca cggctacat cagccccgg agccatcct 
      cgtcggttc tgaaccctg tgctatcga catcgtcaa 
      aaacgcatg ggctagacg cgtcctcca gcctcctcc 
      aggctacca gatcgtggc tttggagga tatcgagtg 
      tgatggtag tccggttgc ccagaccca atggacgcc 
      gcttccgga ggccgtttg tgccgttat cctccggct 
      gtcgcgcca agggcggcc tgctgggat cgagaccat 
      cagggaggc cctcatcat ggccttccc ccacgtctc 
      gcccccaag tgagcaggc cttgtacga cactctggt 
      gcaactgac caccgagag gaagttgta ccatacctc 
      caatgggta ctttatcgt cactccaga acggaggag 
      gtcgcggag cgcgtcacg tggccggaa gcgagtgga 
      ggatcacga ccacgaacc actttccgg taaaacgat 
      gctcatgag tacagaaga agcgcttgt tatggttca 
      tggagacat ggccactat acgagacgg tgatgttac 
      gcaccaact catccaaac caacttggc cgacgttat 
      atgtggagg gaactcgag cgataagat tctctaccc 
      gagaagtac caaacacag ggcctcctc cttatcagt 
      accacgaag tccggaccg gaggggctc cttcaggct 
      gtggaggtc cagccggag gctgaggag gtcggcctc 
      cacgaggac catagccaa acactacgt ggacaacac 
      gagtagtag taccgaaaa ttcagcgcc ttcaacgcc 
      aagtccgaa aaaagagtc gcttttgac gcgcctctt 
      caagaaaac caaacgctc ccagtgcca aatcagctc 
      attcagtgc tcaattccc gccaactga gtggttcaa 
      ctccaaagc cgtccattt acacagcat cgggaccca 
      aacctcgac cacagataa tgaggttgc ctccatcac 
      atcttattt ggaaaactg cgagatcaa accaacacg 
      ttcggccac cctcccccg ccgccgctg ccgccaata 
      ccccgacct accctaccc cccgtaccc cctatcccg 
      tgaaaattc ccccgtcac ccgcaaaaa cccctacat 
      gccttgcac cctcccgcg caacaagca gaaaagccg 
      cgactcctc ccggaagac aagccggag cagttcccg 
      gcaaaccga ggagaaacc cgaaagtgt gaaaactag 
      tcaaaaact ggaagttcc cgacaacag cggaccctc 
      gagcatcga gtcgcataa gagcagcac caagacgga 
      gagctcgtt caacagcgg gccctccag tctcgagtc 
      gcacaaaac cgtcatcaa caccaagga gaaggccaa 
      aaagacaac caacaaccg gacctgcat cccgcaaag 
      caactcgtt cacgctgca agtgacgac caatcaggg 
      cggaaagct gaacaacaa gcccaacgc caccaccac 
      cacgaccat caacgggga aggcggcag cagtacgaa 
      gatcttcgt gcagaattc gccggttcg ctcggttat 
      aaccttcga gaacgggca gtgcgacga ccgcaaccc 
      caacctggt cataatcaa cgacacgga gccgcggga 
      gcaaatccc gacgaaaaa cacgaacca gtccaaaga 
      aacgactct gaacaactc gagagacct gctcccgaa 
      cggcgagag cctcgagca gaagaacaa cagtcgcaa 
      attatcgct tcaacttcc caataacct tataaaaaa 
      cgtctcgac gcacaagtt cgtctccaa ttccaaccc 
      agactcgcc catcatcgg gcacgaagt gcctaaagg 
      gggctacaa cagcacccc tcctagtcc caccaagag 
      caccgacgc cctgtcgat gtcctccat tgggaacat 
      ttacaccaa gaacgaaat gggttcgct ggcgagttt 
      cgattcgtg caaaaagct cgagaaaaa tatgctggg 
      ttctgagcc caatatcag ttttaagga caatcgcga 
      ccaacaatt tcccagtct taatgacct ttcgtttaa 
      ttttacgag tttggcggc acagaaaat cctcaaggg 
      agtgggtct aaatagtgt cgatacgtt ggttgagct 
      gaatatgac ggccaattt ggataaaca gaacaactg 
      tgatgtggt tcatacgga ttttgggat gttttagat 
      ggtcttttt gccggcttt ggacaaagt cggttttaa 
      aggattgta acatatgta tttttagcg agttaggtc 
      agtcaccgg ctgaacatg tatttaata ttttatata 
      cgattttat gtaattatg cagaataaa ctattatat 
      ttaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY