Status
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Gene information
General information
TC033042
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013793,TC013792
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 2910536..2944161
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033042
    iBeetle screen iB_02207
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen potentially transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    iBeetle screen iB_05498
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages randomnot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 66.67% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033042 RA
      MRLVVQMRI SLRNRSAAL RSRPISMQK RPKSAHFYA 
      MQSRTCVRV CECQYKNPS VILIRNATH GVITAAAAE 
      TVRKLEERR DDGLAAKAV GAYYKLTRA HNIFHSGVE 
      MDSQQQQFC LKWNSFGTN LATSFSNLF KSETLADVT 
      LFCDGVTFK AHKLILAAC SKHLADLFE TSPPHQNLI 
      IILDGTSAS NMSALLEFM YKGEVHVSQ DCLSSFLKA 
      AECLQVKGL SIEHEKLAV AQSHMDTTL DSPRKHLKV 
      LKEEMDTSS SQQQSSPSP SYSPAMSPY MHPHHYRQY 
      EQRMPATGA AFESAMKRP LRSPSETLH ERASVLRDG 
      SKTAGRPGS PHGPLSLAF RPSLPLQPE ADAPPENRF 
      DPDPATAVV CPESNTSEC CQDSGCAED LRMKMEPAI 
      QQDEPPNST SGSSGGISN NGSANGSLG QINKYDHER 
      DKDLVPPSL WNSVGKLSH KSGTVNTPD GKKLKCPFC 
      ERLYGYETN LRAHIRQRH QGIRVPCPF CSRTFTRNN 
      TVRRHIARE HKAELSLKA FQNQQQQRP TMAQCHDLS 
      YEDSCDSGY DLSFKSESD DKPEPFDYD FHHPNPDLL 
      DTFTTAFHS TIDSFYTLC AAEAKTSTP VKDKPKKRY 
      GRATRCKSP SQILRIKRV RRLKANDRE RNRMHMLNE 
      ALDRLRCVL PTFPEDTKL TKIETLRFA HSYIFALTQ 
      TLNDLEKYS SSGECVTVN VGNVTVSIG KSGNSITAR 
      NGNAVVTSG SITNASFMQ DYNLEGRHQ DDGFVDQGY 
      ASGNFFNNN CYGSNDFMY EYL
    CDS sequence
    • >TC033042 RA
      atgcgtctc gtggtgcaa atgcggatt tctttacga 
      aaccgcagc gcagcattg aggtcgcga ccgatttcg 
      atgcaaaaa cggccgaaa tcggcgcac ttttacgca 
      atgcagtcg cggacgtgc gtgcgtgtg tgtgagtgc 
      caatataaa aatccatct gtgatactg ataaggaac 
      gcgacgcat ggagtgata acggctgct gctgccgaa 
      acggtgcgt aagctggag gagcgtcgc gacgacggc 
      cttgccgct aaggccgtc ggtgcgtat tacaaactg 
      acacgagct cataatatt ttccattct ggtgttgag 
      atggactct cagcaacaa caattctgt ttgaaatgg 
      aacagtttt ggtacaaat cttgcaaca tcgttcagc 
      aacctcttc aaatccgaa actttggcc gacgtgacg 
      ttattttgt gacggagta acatttaag gcccacaaa 
      ctgatcctg gcagcatgt agtaaacac ctagccgac 
      ttgttcgaa acgtcgccc cctcaccaa aacctaata 
      atcatcctc gatggcacc tcggcgtcc aacatgtcc 
      gccctgttg gagttcatg tacaaaggg gaagtccac 
      gtgtcccaa gactgcctg tccagcttc ctcaaagct 
      gccgaatgc ctccaggtc aagggcctg agcatcgag 
      cacgaaaag ctggccgtg gcccaaagc cacatggac 
      acgacgttg gactcccca cgcaagcac ctcaaagtc 
      ctcaaagag gagatggac acttcctcc agccagcag 
      cagtcgagc ccgtccccc agctactcg cccgccatg 
      tcgccctac atgcacccc catcactac cggcagtac 
      gagcagcgt atgcctgcc acgggggct gccttcgaa 
      tcggcgatg aagcggccg ttgcgcagc cccagcgag 
      actctccac gaacgagcg tcggttttg cgcgatggc 
      agcaaaacg gccggacgt ccgggcagc ccccatggg 
      cccctctcg ctggctttc aggccctcg ttgccactg 
      cagcccgag gctgacgcc ccgccggag aataggttc 
      gatccggat ccggcgacg gcggtggtg tgtccagag 
      agcaatacc tcggagtgc tgccaagac agtggctgt 
      gctgaagat ttgaggatg aaaatggaa ccggctatt 
      caacaagac gaaccgccg aacagtacg agcggtagc 
      agtggaggg atcagcaat aacggttcg gctaatggg 
      tcactggga cagattaat aagtatgat cacgagagg 
      gataaggat ttggtaccg ccgtcgttg tggaattct 
      gtgggaaaa ttgtcgcat aaaagcgga acggttaat 
      acgcccgat ggaaagaaa ttgaagtgc cctttttgt 
      gaacgattg tacggctat gagaccaac ttgcgcgct 
      cacatacga cagcggcac caggggatc cgggtgcct 
      tgtccgttc tgttcacgt actttcacc cgcaataac 
      acagtgagg cggcacata gcccgcgaa cacaaagcc 
      gaattgagt ctgaaggcg tttcagaac cagcaacag 
      cagcgccca acgatggcc cagtgccac gacctctcc 
      tacgaagac agctgcgac tccggttac gacctctcg 
      ttcaaatcg gaaagcgac gacaagccg gagcccttc 
      gactacgac ttccaccac ccgaacccc gacctcctc 
      gacaccttc accaccgcc ttccactcc accatcgac 
      agcttctac acgctgtgc gccgccgag gccaagacc 
      tccaccccc gtcaaggac aagccgaag aagcgctac 
      ggacgcgcc accaggtgc aagagcccc tcgcagatc 
      ctgcgcatc aagcgggtt cgcaggctg aaggccaac 
      gatcgcgag cggaacagg atgcacatg ctgaacgag 
      gcgctggac aggctgagg tgcgttctg ccgacgttt 
      ccggaagac acgaagttg acgaagatt gaaactttg 
      cggtttgct cacagttat atcttcgcg ctgacgcag 
      actttgaac gatttggag aaatattcg agcagtggt 
      gagtgcgtg acggtcaat gtgggcaat gtgaccgtt 
      tcgattggg aaaagtggg aacagtatc acggcaagg 
      aatgggaat gcggtggtt acgagtgga agtatcacg 
      aatgcgagc tttatgcag gattataat ctggaggga 
      cggcatcag gacgacggg tttgtcgat caaggatac 
      gcatcagga aattttttc aataataat tgttacggg 
      agtaacgat tttatgtac gaatatttg taa
    mRNA sequence
    • >TC033042 RA
      gcaccttca agacctggg ctatcggaa aaatgcgtc 
      tcgtggtgc aaatgcgga tttctttac gaaaccgca 
      gcgcagcat tgaggtcgc gaccgattt cgatgcaaa 
      aacggccga aatcggcgc acttttacg caatgcagt 
      cgcggacgt gcgtgcgtg tgtgtgagt gccaatata 
      aaaatccat ctgtgatac tgataagga acgcgacgc 
      atggagtga taacggctg ctgctgccg aaacggtgc 
      gtaagctgg aggagcgtc gcgacgacg gccttgccg 
      ctaaggccg tcggtgcgt attacaaac tgacacgag 
      ctcataata ttttccatt ctggtgttg agatggact 
      ctcagcaac aacaattct gtttgaaat ggaacagtt 
      ttggtacaa atcttgcaa catcgttca gcaacctct 
      tcaaatccg aaactttgg ccgacgtga cgttatttt 
      gtgacggag taacattta aggcccaca aactgatcc 
      tggcagcat gtagtaaac acctagccg acttgttcg 
      aaacgtcgc cccctcacc aaaacctaa taatcatcc 
      tcgatggca cctcggcgt ccaacatgt ccgccctgt 
      tggagttca tgtacaaag gggaagtcc acgtgtccc 
      aagactgcc tgtccagct tcctcaaag ctgccgaat 
      gcctccagg tcaagggcc tgagcatcg agcacgaaa 
      agctggccg tggcccaaa gccacatgg acacgacgt 
      tggactccc cacgcaagc acctcaaag tcctcaaag 
      aggagatgg acacttcct ccagccagc agcagtcga 
      gcccgtccc ccagctact cgcccgcca tgtcgccct 
      acatgcacc cccatcact accggcagt acgagcagc 
      gtatgcctg ccacggggg ctgccttcg aatcggcga 
      tgaagcggc cgttgcgca gccccagcg agactctcc 
      acgaacgag cgtcggttt tgcgcgatg gcagcaaaa 
      cggccggac gtccgggca gcccccatg ggcccctct 
      cgctggctt tcaggccct cgttgccac tgcagcccg 
      aggctgacg ccccgccgg agaataggt tcgatccgg 
      atccggcga cggcggtgg tgtgtccag agagcaata 
      cctcggagt gctgccaag acagtggct gtgctgaag 
      atttgagga tgaaaatgg aaccggcta ttcaacaag 
      acgaaccgc cgaacagta cgagcggta gcagtggag 
      ggatcagca ataacggtt cggctaatg ggtcactgg 
      gacagatta ataagtatg atcacgaga gggataagg 
      atttggtac cgccgtcgt tgtggaatt ctgtgggaa 
      aattgtcgc ataaaagcg gaacggtta atacgcccg 
      atggaaaga aattgaagt gcccttttt gtgaacgat 
      tgtacggct atgagacca acttgcgcg ctcacatac 
      gacagcggc accagggga tccgggtgc cttgtccgt 
      tctgttcac gtactttca cccgcaata acacagtga 
      ggcggcaca tagcccgcg aacacaaag ccgaattga 
      gtctgaagg cgtttcaga accagcaac agcagcgcc 
      caacgatgg cccagtgcc acgacctct cctacgaag 
      acagctgcg actccggtt acgacctct cgttcaaat 
      cggaaagcg acgacaagc cggagccct tcgactacg 
      acttccacc acccgaacc ccgacctcc tcgacacct 
      tcaccaccg ccttccact ccaccatcg acagcttct 
      acacgctgt gcgccgccg aggccaaga cctccaccc 
      ccgtcaagg acaagccga agaagcgct acggacgcg 
      ccaccaggt gcaagagcc cctcgcaga tcctgcgca 
      tcaagcggg ttcgcaggc tgaaggcca acgatcgcg 
      agcggaaca ggatgcaca tgctgaacg aggcgctgg 
      acaggctga ggtgcgttc tgccgacgt ttccggaag 
      acacgaagt tgacgaaga ttgaaactt tgcggtttg 
      ctcacagtt atatcttcg cgctgacgc agactttga 
      acgatttgg agaaatatt cgagcagtg gtgagtgcg 
      tgacggtca atgtgggca atgtgaccg tttcgattg 
      ggaaaagtg ggaacagta tcacggcaa ggaatggga 
      atgcggtgg ttacgagtg gaagtatca cgaatgcga 
      gctttatgc aggattata atctggagg gacggcatc 
      aggacgacg ggtttgtcg atcaaggat acgcatcag 
      gaaattttt tcaataata attgttacg ggagtaacg 
      attttatgt acgaatatt tgtaagact gtgtgataa 
      cgattctag tcaggcctt aagatgtgc tttgtacga 
      aactgtgaa atagattta tatttttat taagattga 
      aatgtattg caatatttt aataaagat ttaaagtta 
      at