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Gene information
General information
TC013791 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4021011..4023339
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013791
    iBeetle screen iB_02206

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 5
    egg chamber slightly number decreased - Number of animals showing the phenotype: 1

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Adult morphology
    elytra slightly surface irregular - Number of animals showing the phenotype: 5


    motility decreased - (22 dpi)
    Days post injection

    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 2
    vitellarium not present - Number of animals showing the phenotype: 2

    tropharium size decreased - Number of animals showing the phenotype: 2
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013791 RA
      MVNLGDVFP NFSADTTVG PIKFHDWLG 
DSWGILFSH PADFTPVCT TELARVVQL 
YPEFAKRGV KVIALSCDS VETHKKWLN 
DIKMYAGYS MEGFPYPLI ADEDRKLAT 
TLQMIDQDE KDSQGIPLT ARAVFIIDA 
KKRMRLSIL YPATTGRNF DEILRVIDS 
LQLTDKHRV ATPVDWKPQ EHVMVQPTV 
SDEEVKTLF PQLTIVALP SGKNYIRRT 
PQP
    CDS sequence
    • >TC013791 RA
      atggttaat ctgggcgac gttttcccg aatttctcg 
gccgacaca accgtcggt ccgatcaaa ttccacgac 
tggcttggc gactcttgg ggcatttta ttttcgcat 
cccgccgac tttacgcca gtttgcaca accgaattg 
gcgcgagtc gtgcaatta taccctgag tttgcaaaa 
cgcggggtt aaagtgatt gcgctctcg tgtgattca 
gttgaaaca cataagaag tggttgaat gatataaaa 
atgtacgct gggtattcc atggagggg tttccgtac 
cccttgata gccgatgaa gatcgtaag ttggccaca 
acgctacaa atgatcgat caagatgag aaagattcg 
caggggatt cccctaact gcacgagcg gttttcata 
attgatgcg aagaagagg atgagactg tcgattttg 
tacccagcg acgactggg aggaatttt gatgaaatt 
ttgcgagtt attgattcg cttcagttg actgataaa 
cacagagtt gcgacgcca gttgattgg aagccccaa 
gagcacgtt atggtacag ccaactgta tcagatgag 
gaagtcaaa actctattc ccacaatta acaatagtt 
gccttacca tcgggaaaa aattacata aggaggacc 
cctcagcct taa
    mRNA sequence
    • >TC013791 RA
      aatcaagcg ataacgttt aattaaaaa aagttataa 
actagcaat ggttaatct gggcgacgt tttcccgaa 
tttctcggc cgacacaac cgtcggtcc gatcaaatt 
ccacgactg gcttggcga ctcttgggg cattttatt 
ttcgcatcc cgccgactt tacgccagt ttgcacaac 
cgaattggc gcgagtcgt gcaattata ccctgagtt 
tgcaaaacg cggggttaa agtgattgc gctctcgtg 
tgattcagt tgaaacaca taagaagtg gttgaatga 
tataaaaat gtacgctgg gtattccat ggaggggtt 
tccgtaccc cttgatagc cgatgaaga tcgtaagtt 
ggccacaac gctacaaat gatcgatca agatgagaa 
agattcgca ggggattcc cctaactgc acgagcggt 
tttcataat tgatgcgaa gaagaggat gagactgtc 
gattttgta cccagcgac gactgggag gaattttga 
tgaaatttt gcgagttat tgattcgct tcagttgac 
tgataaaca cagagttgc gacgccagt tgattggaa 
gccccaaga gcacgttat ggtacagcc aactgtatc 
agatgagga agtcaaaac tctattccc acaattaac 
aatagttgc cttaccatc gggaaaaaa ttacataag 
gaggacccc tcagcctta agttaataa gccaatact 
tttgttatt tttgtacca agattctca aaataaaag 
cttaagtta ctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY