| General information | |||
| TC013784 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 4160775..4185339 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013784 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02205 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
mesothoracic leg musculature pl1-tr1 potentially shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature shape thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
metathoracic leg musculature pl1-tr1 potentially shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC013784 RA
MKKICNKIS KQQWRITVP NINSKKAAT ERKSGSYDL EAAQKEYDR TRTVPNSVF VNSDSDSDT DDEKFQDAM ENGTSYLNE PTEPVEYDV PKISFKFLS KGEPPENDV VLKSEVCFE VATPEPSPV KSDIEILPV VGIIKCGSD PNLVLRDHE DDGLYKIPR QLARCRLSQ SLNDFKVEE FQAYTSNHS SIEHVSCSQ VIVQSPDVP PENFDYCKA RSKLPMKLR RPTFLRKPK KAVDTWNQI RTKFSNIMI EHAAQQRVG AFSDKDKVT LNLEEVYKT SKTKCKNAF KSTTRMFKR NQDSLDSEN SGQFVKNEA FFARVPLTP NDIEYKFNF NNEKGTNDD EKAEFDFSS IKSAFRRSK FVAEGQNGF EDLRRYVKQ GGDFCKELA TILQERAEA ETQYAKNLS KLSSKLSRA CRDGVGGLN EAWKAVAIE LETRAEIHR LMGQALSEE TAKPLRNMT ENQHRTRKQ CEGTVDKAA KSLAEWRTA EAKSKKQSH TCARDNEKL QDAAVLDTS RSGKLTSSS LIQLAHMHN KNTPDKENT KLEGKKRKA EDAVKKADI EYYTLCVRA ERARLEWES AVLRGVNTF QSLEEERLN HLKTVLTSY LHHSAELGP RLIEASERL KAPVTQAES GKDLSTFIN LRQSSQQVS EQLLPDFYC EHITLAMNR ERRKQALVK LLHLIRQDI ERERKSKNG LENLSKAIK QTPNFGAED SQQSVSEKL YHMKSMLTY LEGARYKLH NALAELDSK PRSGHPLAA HITITRDKS GLQQSILKV PQWLKEEWF ETEKSPLSE KSVRSSKSD NSDPHNNDI ADPEWTDRG AADGNSNQP DSDFDEFSS QCSSAENIC NTEESPVQP IAQCKALYA YTPNLTDEL ALQPGDLLS VYRQQDDGW WLGECKGNV GIFPATYVE LINSN
- >TC013784 RB
MKKICNKIS KQQWRITVP NINSKKAAT ERKSGSYDL EAAQKEYDR TRTVPNSVF VNSDSDSDT DDEKFQDAM ENGTSYLNE PTEPVEYDV PKISFKFLS KGEPPENDV VLKSEVCFE VATPEPSPV KSDIEILPV VGIIKCGSD PNLVLRDHE DDGLYKIPR QLARCRLSQ SLNDFKVEE FQAYTSNHS SIEHVSCSQ VIVQSPDVP PENFDYCKA RSKLPMKLR RPTFLRKPK KAVDTWNQI RTKFSNIMI EHAAQQRVG AFSDKDKVT LNLEEVYKT SKTKCKNAF KSTTRMFKR NQDSLDSEN SGQFVKNEA FFARVPLTP NDIEYKFNF NNEKGTNDD EKAEFDFSS IKSAFRRSK FVAEGQNGF EDLRRYVKQ GGDFCKELA TILQERAEA ETQYAKNLS KLSSKLSRA CRDGVGGLN EAWKAVAIE LETRAEIHR LMGQALSEE TAKPLRNMT ENQHRTRKQ CEGTVDKAA KSLAEWRTA EAKSKKQSH TCARDNEKL QDAAVLDTS RSGKLTLNR SSSLIQLAH MHNKNTPDK ENTKLEGKK RKAEDAVKK ADIEYYTLC VRAERARLE WESAVLRGV NTFQSLEEE RLNHLKTVL TSYLHHSAE LGPRLIEAS ERLKAPVTQ AESGKDLST FINLRQSSQ QVSEQLLPD FYCEHITLA MNRERRKQA LVKLLHLIR QDIERERKS KNGLENLSK AIKQTPNFG AEDSQQSVS EKLYHMKSM LTYLEGARY KLHNALAEL DSKPRSGHP LAAHITITR DKSGLQQSI LKVPQWLKE EWFETEKSP LSEKSVRSS KSDNSDPHN NDIADPEWT DRGAADGNS NQPDSDFDE FSSQCSSAE NICNTEESP VQPIAQCKA LYAYTPNLT DELALQPGD LLSVYRQQD DGWWLGECK GNVGIFPAT YVELINSN
- >TC013784 RA
atgaaaaaa atctgcaat aaaatatcg aaacagcag tggagaatc accgttccg aatatcaac tcgaaaaaa gccgctacc gagcgaaaa tcgggctct tacgacttg gaagcggcc caaaaggaa tacgaccgc actagaact gtgccaaac agtgttttt gtaaacagt gatagtgat agtgacacc gacgacgaa aaattccaa gacgcaatg gaaaatggt acaagttac ctgaacgaa ccaacggaa ccagtggaa tacgacgtc cccaaaatc agcttcaaa tttctatca aagggtgaa ccccccgaa aacgatgtg gtgctcaaa agtgaagtg tgcttcgaa gtcgcaacc cccgaaccc tcgcccgtt aaaagtgac atcgaaatt ttgccagtg gtgggcatc atcaagtgc ggaagtgac ccaaacctg gtgcttcgt gaccacgaa gacgacggt ctttacaaa atcccgaga caattggcc aggtgtcgt ctatcacaa tctttaaac gatttcaaa gtcgaggag ttccaagcc tacacttcg aaccactcg agcatcgaa catgtctcc tgtagtcag gttattgtg caaagtccg gacgtacca ccggagaat ttcgactat tgcaaagcc cggagtaaa ttaccgatg aagttacgg aggcccact tttttgaga aaacccaaa aaagcagtc gatacttgg aaccaaatc cggacgaag tttagcaat attatgatc gaacatgcg gcccagcag cgagttggg gcgttcagt gataaggat aaggtcact cttaatctt gaagaagtg tacaaaacg tccaaaacc aagtgcaag aacgcgttt aaaagcaca acgaggatg ttcaagcgg aatcaggat tcgcttgat agtgaaaat tcggggcag tttgttaaa aatgaggcg ttttttgca agagtgccg ctcacgccg aatgatatt gagtataag tttaatttc aacaatgaa aagggcacc aatgatgat gagaaggct gagtttgat ttttcatcg attaaatcg gcgtttcgg agaagtaaa ttcgtggcg gaggggcaa aatgggttt gaagatctt agaagatac gtgaaacag gggggcgat ttctgcaag gaactcgcc accatactc caagaaagg gccgaagct gagacccaa tacgccaaa aacttatca aaactcagc tcaaaacta tcccgagca tgtcgcgac ggcgtcggc ggcctaaac gaagcctgg aaagccgtc gctattgaa ctggaaact cgggctgaa atccaccgc ctgatgggc caagccctc tcggaggag acggcgaaa ccgctccgg aacatgacg gagaaccag caccggacc cggaaacag tgcgaaggg acggtggac aaagcggcc aaatccttg gccgaatgg aggacagct gaggccaag agcaagaag caaagccat acgtgtgcc agagacaac gaaaagttg caagatgcc gccgttttg gatacgtcg aggtcgggg aagttgacg agcagcagt ttgatccag ctggcgcac atgcacaat aaaaacaca ccggataag gaaaatacg aagctggaa gggaagaag cgcaaggcg gaggatgca gtcaagaag gcggatatc gagtattac acgctttgc gtccgggca gagagggca agactggaa tgggaatcg gccgttttg cggggcgtc aacacgttt cagtcgctt gaagaagag agattgaat catctgaaa accgttcta acgtcgtat cttcatcac agtgccgaa ctagggcct agactaata gaagcatct gagagactc aaagccccg gtcacgcaa gctgaatcg ggtaaagat ttgagcact tttattaat ttgagacaa tcctcgcaa caagtctcg gaacagctt ttgccggat ttttactgc gaacatatt actttggct atgaatagg gaacggcgg aagcaggct ttggtcaaa ttactacac ctaatccgg caagatatc gaaagggag aggaagtct aagaatggt ttggaaaac ttatctaaa gccatcaag caaacccca aattttggt gctgaggat tcgcaacaa agtgtcagc gaaaaactg tatcatatg aagtcgatg ttaacgtat ttggaggga gcacgatac aaactacac aatgcttta gcagaactt gacagtaaa ccaaggtct gggcatccc ctagcagcc catattacc ataacgagg gataaatcg ggattgcaa caaagcata cttaaggtg cctcagtgg ttgaaagaa gaatggttc gagacggag aagagcccc ctgagtgaa aaatccgtc agatcaagc aaatcggat aattccgat ccgcacaac aatgacata gccgacccc gaatggacc gacaggggg gctgctgac ggcaactct aatcagcct gactccgat ttcgatgag ttttcgtct caatgcagc agtgctgaa aacatttgc aacacggaa gagagtcca gtgcagcca atcgcacaa tgcaaagct ctgtacgct tacactccc aatttaacc gacgagttg gcgttacaa ccaggcgat ctcctctcg gtttatcgg cagcaagac gacggctgg tggctggga gagtgcaaa ggaaacgtc gggattttc cccgcaact tacgtcgag ctgatcaat tcgaactaa
- >TC013784 RB
atgaaaaaa atctgcaat aaaatatcg aaacagcag tggagaatc accgttccg aatatcaac tcgaaaaaa gccgctacc gagcgaaaa tcgggctct tacgacttg gaagcggcc caaaaggaa tacgaccgc actagaact gtgccaaac agtgttttt gtaaacagt gatagtgat agtgacacc gacgacgaa aaattccaa gacgcaatg gaaaatggt acaagttac ctgaacgaa ccaacggaa ccagtggaa tacgacgtc cccaaaatc agcttcaaa tttctatca aagggtgaa ccccccgaa aacgatgtg gtgctcaaa agtgaagtg tgcttcgaa gtcgcaacc cccgaaccc tcgcccgtt aaaagtgac atcgaaatt ttgccagtg gtgggcatc atcaagtgc ggaagtgac ccaaacctg gtgcttcgt gaccacgaa gacgacggt ctttacaaa atcccgaga caattggcc aggtgtcgt ctatcacaa tctttaaac gatttcaaa gtcgaggag ttccaagcc tacacttcg aaccactcg agcatcgaa catgtctcc tgtagtcag gttattgtg caaagtccg gacgtacca ccggagaat ttcgactat tgcaaagcc cggagtaaa ttaccgatg aagttacgg aggcccact tttttgaga aaacccaaa aaagcagtc gatacttgg aaccaaatc cggacgaag tttagcaat attatgatc gaacatgcg gcccagcag cgagttggg gcgttcagt gataaggat aaggtcact cttaatctt gaagaagtg tacaaaacg tccaaaacc aagtgcaag aacgcgttt aaaagcaca acgaggatg ttcaagcgg aatcaggat tcgcttgat agtgaaaat tcggggcag tttgttaaa aatgaggcg ttttttgca agagtgccg ctcacgccg aatgatatt gagtataag tttaatttc aacaatgaa aagggcacc aatgatgat gagaaggct gagtttgat ttttcatcg attaaatcg gcgtttcgg agaagtaaa ttcgtggcg gaggggcaa aatgggttt gaagatctt agaagatac gtgaaacag gggggcgat ttctgcaag gaactcgcc accatactc caagaaagg gccgaagct gagacccaa tacgccaaa aacttatca aaactcagc tcaaaacta tcccgagca tgtcgcgac ggcgtcggc ggcctaaac gaagcctgg aaagccgtc gctattgaa ctggaaact cgggctgaa atccaccgc ctgatgggc caagccctc tcggaggag acggcgaaa ccgctccgg aacatgacg gagaaccag caccggacc cggaaacag tgcgaaggg acggtggac aaagcggcc aaatccttg gccgaatgg aggacagct gaggccaag agcaagaag caaagccat acgtgtgcc agagacaac gaaaagttg caagatgcc gccgttttg gatacgtcg aggtcgggg aagttgacg ttgaatagg agcagcagt ttgatccag ctggcgcac atgcacaat aaaaacaca ccggataag gaaaatacg aagctggaa gggaagaag cgcaaggcg gaggatgca gtcaagaag gcggatatc gagtattac acgctttgc gtccgggca gagagggca agactggaa tgggaatcg gccgttttg cggggcgtc aacacgttt cagtcgctt gaagaagag agattgaat catctgaaa accgttcta acgtcgtat cttcatcac agtgccgaa ctagggcct agactaata gaagcatct gagagactc aaagccccg gtcacgcaa gctgaatcg ggtaaagat ttgagcact tttattaat ttgagacaa tcctcgcaa caagtctcg gaacagctt ttgccggat ttttactgc gaacatatt actttggct atgaatagg gaacggcgg aagcaggct ttggtcaaa ttactacac ctaatccgg caagatatc gaaagggag aggaagtct aagaatggt ttggaaaac ttatctaaa gccatcaag caaacccca aattttggt gctgaggat tcgcaacaa agtgtcagc gaaaaactg tatcatatg aagtcgatg ttaacgtat ttggaggga gcacgatac aaactacac aatgcttta gcagaactt gacagtaaa ccaaggtct gggcatccc ctagcagcc catattacc ataacgagg gataaatcg ggattgcaa caaagcata cttaaggtg cctcagtgg ttgaaagaa gaatggttc gagacggag aagagcccc ctgagtgaa aaatccgtc agatcaagc aaatcggat aattccgat ccgcacaac aatgacata gccgacccc gaatggacc gacaggggg gctgctgac ggcaactct aatcagcct gactccgat ttcgatgag ttttcgtct caatgcagc agtgctgaa aacatttgc aacacggaa gagagtcca gtgcagcca atcgcacaa tgcaaagct ctgtacgct tacactccc aatttaacc gacgagttg gcgttacaa ccaggcgat ctcctctcg gtttatcgg cagcaagac gacggctgg tggctggga gagtgcaaa ggaaacgtc gggattttc cccgcaact tacgtcgag ctgatcaat tcgaactaa
- >TC013784 RA
cacacacac atgtgataa ttatagcac cacagacta atatagtga ggtcttgct ccgcgttgg ccctgcggt agttctgaa tgaagcgcg agtgcgaaa agtgaaaaa tgtgaactt tcgagtgtt gtgttatgt caccgtcat atgatagca agcacaata tgcgacctt cccaataat ttaccttta acaatttca tcgacgtca acattttcc ttccttatc taaccgtac acacaacct aattagcgt ttatcctgc ttttttcac aaatgagtg tcagtaagt agcggcaat tgagtgcga cgcgagcga aaatgaaaa aaatctgca ataaaatat cgaaacagc agtggagaa tcaccgttc cgaatatca actcgaaaa aagccgcta ccgagcgaa aatcgggct cttacgact tggaagcgg cccaaaagg aatacgacc gcactagaa ctgtgccaa acagtgttt ttgtaaaca gtgatagtg atagtgaca ccgacgacg aaaaattcc aagacgcaa tggaaaatg gtacaagtt acctgaacg aaccaacgg aaccagtgg aatacgacg tccccaaaa tcagcttca aatttctat caaagggtg aaccccccg aaaacgatg tggtgctca aaagtgaag tgtgcttcg aagtcgcaa cccccgaac cctcgcccg ttaaaagtg acatcgaaa ttttgccag tggtgggca tcatcaagt gcggaagtg acccaaacc tggtgcttc gtgaccacg aagacgacg gtctttaca aaatcccga gacaattgg ccaggtgtc gtctatcac aatctttaa acgatttca aagtcgagg agttccaag cctacactt cgaaccact cgagcatcg aacatgtct cctgtagtc aggttattg tgcaaagtc cggacgtac caccggaga atttcgact attgcaaag cccggagta aattaccga tgaagttac ggaggccca cttttttga gaaaaccca aaaaagcag tcgatactt ggaaccaaa tccggacga agtttagca atattatga tcgaacatg cggcccagc agcgagttg gggcgttca gtgataagg ataaggtca ctcttaatc ttgaagaag tgtacaaaa cgtccaaaa ccaagtgca agaacgcgt ttaaaagca caacgagga tgttcaagc ggaatcagg attcgcttg atagtgaaa attcggggc agtttgtta aaaatgagg cgttttttg caagagtgc cgctcacgc cgaatgata ttgagtata agtttaatt tcaacaatg aaaagggca ccaatgatg atgagaagg ctgagtttg atttttcat cgattaaat cggcgtttc ggagaagta aattcgtgg cggaggggc aaaatgggt ttgaagatc ttagaagat acgtgaaac aggggggcg atttctgca aggaactcg ccaccatac tccaagaaa gggccgaag ctgagaccc aatacgcca aaaacttat caaaactca gctcaaaac tatcccgag catgtcgcg acggcgtcg gcggcctaa acgaagcct ggaaagccg tcgctattg aactggaaa ctcgggctg aaatccacc gcctgatgg gccaagccc tctcggagg agacggcga aaccgctcc ggaacatga cggagaacc agcaccgga cccggaaac agtgcgaag ggacggtgg acaaagcgg ccaaatcct tggccgaat ggaggacag ctgaggcca agagcaaga agcaaagcc atacgtgtg ccagagaca acgaaaagt tgcaagatg ccgccgttt tggatacgt cgaggtcgg ggaagttga cgagcagca gtttgatcc agctggcgc acatgcaca ataaaaaca caccggata aggaaaata cgaagctgg aagggaaga agcgcaagg cggaggatg cagtcaaga aggcggata tcgagtatt acacgcttt gcgtccggg cagagaggg caagactgg aatgggaat cggccgttt tgcggggcg tcaacacgt ttcagtcgc ttgaagaag agagattga atcatctga aaaccgttc taacgtcgt atcttcatc acagtgccg aactagggc ctagactaa tagaagcat ctgagagac tcaaagccc cggtcacgc aagctgaat cgggtaaag atttgagca cttttatta atttgagac aatcctcgc aacaagtct cggaacagc ttttgccgg atttttact gcgaacata ttactttgg ctatgaata gggaacggc ggaagcagg ctttggtca aattactac acctaatcc ggcaagata tcgaaaggg agaggaagt ctaagaatg gtttggaaa acttatcta aagccatca agcaaaccc caaattttg gtgctgagg attcgcaac aaagtgtca gcgaaaaac tgtatcata tgaagtcga tgttaacgt atttggagg gagcacgat acaaactac acaatgctt tagcagaac ttgacagta aaccaaggt ctgggcatc ccctagcag cccatatta ccataacga gggataaat cgggattgc aacaaagca tacttaagg tgcctcagt ggttgaaag aagaatggt tcgagacgg agaagagcc ccctgagtg aaaaatccg tcagatcaa gcaaatcgg ataattccg atccgcaca acaatgaca tagccgacc ccgaatgga ccgacaggg gggctgctg acggcaact ctaatcagc ctgactccg atttcgatg agttttcgt ctcaatgca gcagtgctg aaaacattt gcaacacgg aagagagtc cagtgcagc caatcgcac aatgcaaag ctctgtacg cttacactc ccaatttaa ccgacgagt tggcgttac aaccaggcg atctcctct cggtttatc ggcagcaag acgacggct ggtggctgg gagagtgca aaggaaacg tcgggattt tccccgcaa cttacgtcg agctgatca attcgaact aaagactgg agaacgatt ccacgttgg gataaacgc tgtacaaat aattttaac gttcttccc tccccactt tatacaaat tttttattc acaacttat taatatttt gttaacagc agtgataat aaaagtgta actcctcc
- >TC013784 RB
cacacacac atgtgataa ttatagcac cacagacta atatagtga ggtcttgct ccgcgttgg ccctgcggt agttctgaa tgaagcgcg agtgcgaaa agtgaaaaa tgtgaactt tcgagtgtt gtgttatgt caccgtcat atgatagca agcacaata tgcgacctt cccaataat ttaccttta acaatttca tcgacgtca acattttcc ttccttatc taaccgtac acacaacct aattagcgt ttatcctgc ttttttcac aaatgagtg tcagtaagt agcggcaat tgagtgcga cgcgagcga aaatgaaaa aaatctgca ataaaatat cgaaacagc agtggagaa tcaccgttc cgaatatca actcgaaaa aagccgcta ccgagcgaa aatcgggct cttacgact tggaagcgg cccaaaagg aatacgacc gcactagaa ctgtgccaa acagtgttt ttgtaaaca gtgatagtg atagtgaca ccgacgacg aaaaattcc aagacgcaa tggaaaatg gtacaagtt acctgaacg aaccaacgg aaccagtgg aatacgacg tccccaaaa tcagcttca aatttctat caaagggtg aaccccccg aaaacgatg tggtgctca aaagtgaag tgtgcttcg aagtcgcaa cccccgaac cctcgcccg ttaaaagtg acatcgaaa ttttgccag tggtgggca tcatcaagt gcggaagtg acccaaacc tggtgcttc gtgaccacg aagacgacg gtctttaca aaatcccga gacaattgg ccaggtgtc gtctatcac aatctttaa acgatttca aagtcgagg agttccaag cctacactt cgaaccact cgagcatcg aacatgtct cctgtagtc aggttattg tgcaaagtc cggacgtac caccggaga atttcgact attgcaaag cccggagta aattaccga tgaagttac ggaggccca cttttttga gaaaaccca aaaaagcag tcgatactt ggaaccaaa tccggacga agtttagca atattatga tcgaacatg cggcccagc agcgagttg gggcgttca gtgataagg ataaggtca ctcttaatc ttgaagaag tgtacaaaa cgtccaaaa ccaagtgca agaacgcgt ttaaaagca caacgagga tgttcaagc ggaatcagg attcgcttg atagtgaaa attcggggc agtttgtta aaaatgagg cgttttttg caagagtgc cgctcacgc cgaatgata ttgagtata agtttaatt tcaacaatg aaaagggca ccaatgatg atgagaagg ctgagtttg atttttcat cgattaaat cggcgtttc ggagaagta aattcgtgg cggaggggc aaaatgggt ttgaagatc ttagaagat acgtgaaac aggggggcg atttctgca aggaactcg ccaccatac tccaagaaa gggccgaag ctgagaccc aatacgcca aaaacttat caaaactca gctcaaaac tatcccgag catgtcgcg acggcgtcg gcggcctaa acgaagcct ggaaagccg tcgctattg aactggaaa ctcgggctg aaatccacc gcctgatgg gccaagccc tctcggagg agacggcga aaccgctcc ggaacatga cggagaacc agcaccgga cccggaaac agtgcgaag ggacggtgg acaaagcgg ccaaatcct tggccgaat ggaggacag ctgaggcca agagcaaga agcaaagcc atacgtgtg ccagagaca acgaaaagt tgcaagatg ccgccgttt tggatacgt cgaggtcgg ggaagttga cgttgaata ggagcagca gtttgatcc agctggcgc acatgcaca ataaaaaca caccggata aggaaaata cgaagctgg aagggaaga agcgcaagg cggaggatg cagtcaaga aggcggata tcgagtatt acacgcttt gcgtccggg cagagaggg caagactgg aatgggaat cggccgttt tgcggggcg tcaacacgt ttcagtcgc ttgaagaag agagattga atcatctga aaaccgttc taacgtcgt atcttcatc acagtgccg aactagggc ctagactaa tagaagcat ctgagagac tcaaagccc cggtcacgc aagctgaat cgggtaaag atttgagca cttttatta atttgagac aatcctcgc aacaagtct cggaacagc ttttgccgg atttttact gcgaacata ttactttgg ctatgaata gggaacggc ggaagcagg ctttggtca aattactac acctaatcc ggcaagata tcgaaaggg agaggaagt ctaagaatg gtttggaaa acttatcta aagccatca agcaaaccc caaattttg gtgctgagg attcgcaac aaagtgtca gcgaaaaac tgtatcata tgaagtcga tgttaacgt atttggagg gagcacgat acaaactac acaatgctt tagcagaac ttgacagta aaccaaggt ctgggcatc ccctagcag cccatatta ccataacga gggataaat cgggattgc aacaaagca tacttaagg tgcctcagt ggttgaaag aagaatggt tcgagacgg agaagagcc ccctgagtg aaaaatccg tcagatcaa gcaaatcgg ataattccg atccgcaca acaatgaca tagccgacc ccgaatgga ccgacaggg gggctgctg acggcaact ctaatcagc ctgactccg atttcgatg agttttcgt ctcaatgca gcagtgctg aaaacattt gcaacacgg aagagagtc cagtgcagc caatcgcac aatgcaaag ctctgtacg cttacactc ccaatttaa ccgacgagt tggcgttac aaccaggcg atctcctct cggtttatc ggcagcaag acgacggct ggtggctgg gagagtgca aaggaaacg tcgggattt tccccgcaa cttacgtcg agctgatca attcgaact aaagactgg agaacgatt ccacgttgg gataaacgc tgtacaaat aattttaac gttcttccc tccccactt tatacaaat tttttattc acaacttat taatatttt gttaacagc agtgataat aaaagtgta actcctcc
