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Gene information
General information
TC013784 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 3125567..3150131
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013784
    iBeetle screen iB_02205
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    mesothoracic leg musculature pl1-tr1 potentially shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature shape thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    metathoracic leg musculature pl1-tr1 potentially shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Pupal morphology
    oblique dorsal thoracic muscles need term anteriorshape irregular - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013784 RA
      MKKICNKIS KQQWRITVP NINSKKAAT ERKSGSYDL 
      EAAQKEYDR TRTVPNSVF VNSDSDSDT DDEKFQDAM 
      ENGTSYLNE PTEPVEYDV PKISFKFLS KGEPPENDV 
      VLKSEVCFE VATPEPSPV KSDIEILPV VGIIKCGSD 
      PNLVLRDHE DDGLYKIPR QLARCRLSQ SLNDFKVEE 
      FQAYTSNHS SIEHVSCSQ VIVQSPDVP PENFDYCKA 
      RSKLPMKLR RPTFLRKPK KAVDTWNQI RTKFSNIMI 
      EHAAQQRVG AFSDKDKVT LNLEEVYKT SKTKCKNAF 
      KSTTRMFKR NQDSLDSEN SGQFVKNEA FFARVPLTP 
      NDIEYKFNF NNEKGTNDD EKAEFDFSS IKSAFRRSK 
      FVAEGQNGF EDLRRYVKQ GGDFCKELA TILQERAEA 
      ETQYAKNLS KLSSKLSRA CRDGVGGLN EAWKAVAIE 
      LETRAEIHR LMGQALSEE TAKPLRNMT ENQHRTRKQ 
      CEGTVDKAA KSLAEWRTA EAKSKKQSH TCARDNEKL 
      QDAAVLDTS RSGKLTSSS LIQLAHMHN KNTPDKENT 
      KLEGKKRKA EDAVKKADI EYYTLCVRA ERARLEWES 
      AVLRGVNTF QSLEEERLN HLKTVLTSY LHHSAELGP 
      RLIEASERL KAPVTQAES GKDLSTFIN LRQSSQQVS 
      EQLLPDFYC EHITLAMNR ERRKQALVK LLHLIRQDI 
      ERERKSKNG LENLSKAIK QTPNFGAED SQQSVSEKL 
      YHMKSMLTY LEGARYKLH NALAELDSK PRSGHPLAA 
      HITITRDKS GLQQSILKV PQWLKEEWF ETEKSPLSE 
      KSVRSSKSD NSDPHNNDI ADPEWTDRG AADGNSNQP 
      DSDFDEFSS QCSSAENIC NTEESPVQP IAQCKALYA 
      YTPNLTDEL ALQPGDLLS VYRQQDDGW WLGECKGNV 
      GIFPATYVE LINSN
    • >TC013784 RB
      MKKICNKIS KQQWRITVP NINSKKAAT ERKSGSYDL 
      EAAQKEYDR TRTVPNSVF VNSDSDSDT DDEKFQDAM 
      ENGTSYLNE PTEPVEYDV PKISFKFLS KGEPPENDV 
      VLKSEVCFE VATPEPSPV KSDIEILPV VGIIKCGSD 
      PNLVLRDHE DDGLYKIPR QLARCRLSQ SLNDFKVEE 
      FQAYTSNHS SIEHVSCSQ VIVQSPDVP PENFDYCKA 
      RSKLPMKLR RPTFLRKPK KAVDTWNQI RTKFSNIMI 
      EHAAQQRVG AFSDKDKVT LNLEEVYKT SKTKCKNAF 
      KSTTRMFKR NQDSLDSEN SGQFVKNEA FFARVPLTP 
      NDIEYKFNF NNEKGTNDD EKAEFDFSS IKSAFRRSK 
      FVAEGQNGF EDLRRYVKQ GGDFCKELA TILQERAEA 
      ETQYAKNLS KLSSKLSRA CRDGVGGLN EAWKAVAIE 
      LETRAEIHR LMGQALSEE TAKPLRNMT ENQHRTRKQ 
      CEGTVDKAA KSLAEWRTA EAKSKKQSH TCARDNEKL 
      QDAAVLDTS RSGKLTLNR SSSLIQLAH MHNKNTPDK 
      ENTKLEGKK RKAEDAVKK ADIEYYTLC VRAERARLE 
      WESAVLRGV NTFQSLEEE RLNHLKTVL TSYLHHSAE 
      LGPRLIEAS ERLKAPVTQ AESGKDLST FINLRQSSQ 
      QVSEQLLPD FYCEHITLA MNRERRKQA LVKLLHLIR 
      QDIERERKS KNGLENLSK AIKQTPNFG AEDSQQSVS 
      EKLYHMKSM LTYLEGARY KLHNALAEL DSKPRSGHP 
      LAAHITITR DKSGLQQSI LKVPQWLKE EWFETEKSP 
      LSEKSVRSS KSDNSDPHN NDIADPEWT DRGAADGNS 
      NQPDSDFDE FSSQCSSAE NICNTEESP VQPIAQCKA 
      LYAYTPNLT DELALQPGD LLSVYRQQD DGWWLGECK 
      GNVGIFPAT YVELINSN
    CDS sequence
    • >TC013784 RA
      atgaaaaaa atctgcaat aaaatatcg aaacagcag 
      tggagaatc accgttccg aatatcaac tcgaaaaaa 
      gccgctacc gagcgaaaa tcgggctct tacgacttg 
      gaagcggcc caaaaggaa tacgaccgc actagaact 
      gtgccaaac agtgttttt gtaaacagt gatagtgat 
      agtgacacc gacgacgaa aaattccaa gacgcaatg 
      gaaaatggt acaagttac ctgaacgaa ccaacggaa 
      ccagtggaa tacgacgtc cccaaaatc agcttcaaa 
      tttctatca aagggtgaa ccccccgaa aacgatgtg 
      gtgctcaaa agtgaagtg tgcttcgaa gtcgcaacc 
      cccgaaccc tcgcccgtt aaaagtgac atcgaaatt 
      ttgccagtg gtgggcatc atcaagtgc ggaagtgac 
      ccaaacctg gtgcttcgt gaccacgaa gacgacggt 
      ctttacaaa atcccgaga caattggcc aggtgtcgt 
      ctatcacaa tctttaaac gatttcaaa gtcgaggag 
      ttccaagcc tacacttcg aaccactcg agcatcgaa 
      catgtctcc tgtagtcag gttattgtg caaagtccg 
      gacgtacca ccggagaat ttcgactat tgcaaagcc 
      cggagtaaa ttaccgatg aagttacgg aggcccact 
      tttttgaga aaacccaaa aaagcagtc gatacttgg 
      aaccaaatc cggacgaag tttagcaat attatgatc 
      gaacatgcg gcccagcag cgagttggg gcgttcagt 
      gataaggat aaggtcact cttaatctt gaagaagtg 
      tacaaaacg tccaaaacc aagtgcaag aacgcgttt 
      aaaagcaca acgaggatg ttcaagcgg aatcaggat 
      tcgcttgat agtgaaaat tcggggcag tttgttaaa 
      aatgaggcg ttttttgca agagtgccg ctcacgccg 
      aatgatatt gagtataag tttaatttc aacaatgaa 
      aagggcacc aatgatgat gagaaggct gagtttgat 
      ttttcatcg attaaatcg gcgtttcgg agaagtaaa 
      ttcgtggcg gaggggcaa aatgggttt gaagatctt 
      agaagatac gtgaaacag gggggcgat ttctgcaag 
      gaactcgcc accatactc caagaaagg gccgaagct 
      gagacccaa tacgccaaa aacttatca aaactcagc 
      tcaaaacta tcccgagca tgtcgcgac ggcgtcggc 
      ggcctaaac gaagcctgg aaagccgtc gctattgaa 
      ctggaaact cgggctgaa atccaccgc ctgatgggc 
      caagccctc tcggaggag acggcgaaa ccgctccgg 
      aacatgacg gagaaccag caccggacc cggaaacag 
      tgcgaaggg acggtggac aaagcggcc aaatccttg 
      gccgaatgg aggacagct gaggccaag agcaagaag 
      caaagccat acgtgtgcc agagacaac gaaaagttg 
      caagatgcc gccgttttg gatacgtcg aggtcgggg 
      aagttgacg agcagcagt ttgatccag ctggcgcac 
      atgcacaat aaaaacaca ccggataag gaaaatacg 
      aagctggaa gggaagaag cgcaaggcg gaggatgca 
      gtcaagaag gcggatatc gagtattac acgctttgc 
      gtccgggca gagagggca agactggaa tgggaatcg 
      gccgttttg cggggcgtc aacacgttt cagtcgctt 
      gaagaagag agattgaat catctgaaa accgttcta 
      acgtcgtat cttcatcac agtgccgaa ctagggcct 
      agactaata gaagcatct gagagactc aaagccccg 
      gtcacgcaa gctgaatcg ggtaaagat ttgagcact 
      tttattaat ttgagacaa tcctcgcaa caagtctcg 
      gaacagctt ttgccggat ttttactgc gaacatatt 
      actttggct atgaatagg gaacggcgg aagcaggct 
      ttggtcaaa ttactacac ctaatccgg caagatatc 
      gaaagggag aggaagtct aagaatggt ttggaaaac 
      ttatctaaa gccatcaag caaacccca aattttggt 
      gctgaggat tcgcaacaa agtgtcagc gaaaaactg 
      tatcatatg aagtcgatg ttaacgtat ttggaggga 
      gcacgatac aaactacac aatgcttta gcagaactt 
      gacagtaaa ccaaggtct gggcatccc ctagcagcc 
      catattacc ataacgagg gataaatcg ggattgcaa 
      caaagcata cttaaggtg cctcagtgg ttgaaagaa 
      gaatggttc gagacggag aagagcccc ctgagtgaa 
      aaatccgtc agatcaagc aaatcggat aattccgat 
      ccgcacaac aatgacata gccgacccc gaatggacc 
      gacaggggg gctgctgac ggcaactct aatcagcct 
      gactccgat ttcgatgag ttttcgtct caatgcagc 
      agtgctgaa aacatttgc aacacggaa gagagtcca 
      gtgcagcca atcgcacaa tgcaaagct ctgtacgct 
      tacactccc aatttaacc gacgagttg gcgttacaa 
      ccaggcgat ctcctctcg gtttatcgg cagcaagac 
      gacggctgg tggctggga gagtgcaaa ggaaacgtc 
      gggattttc cccgcaact tacgtcgag ctgatcaat 
      tcgaactaa
    • >TC013784 RB
      atgaaaaaa atctgcaat aaaatatcg aaacagcag 
      tggagaatc accgttccg aatatcaac tcgaaaaaa 
      gccgctacc gagcgaaaa tcgggctct tacgacttg 
      gaagcggcc caaaaggaa tacgaccgc actagaact 
      gtgccaaac agtgttttt gtaaacagt gatagtgat 
      agtgacacc gacgacgaa aaattccaa gacgcaatg 
      gaaaatggt acaagttac ctgaacgaa ccaacggaa 
      ccagtggaa tacgacgtc cccaaaatc agcttcaaa 
      tttctatca aagggtgaa ccccccgaa aacgatgtg 
      gtgctcaaa agtgaagtg tgcttcgaa gtcgcaacc 
      cccgaaccc tcgcccgtt aaaagtgac atcgaaatt 
      ttgccagtg gtgggcatc atcaagtgc ggaagtgac 
      ccaaacctg gtgcttcgt gaccacgaa gacgacggt 
      ctttacaaa atcccgaga caattggcc aggtgtcgt 
      ctatcacaa tctttaaac gatttcaaa gtcgaggag 
      ttccaagcc tacacttcg aaccactcg agcatcgaa 
      catgtctcc tgtagtcag gttattgtg caaagtccg 
      gacgtacca ccggagaat ttcgactat tgcaaagcc 
      cggagtaaa ttaccgatg aagttacgg aggcccact 
      tttttgaga aaacccaaa aaagcagtc gatacttgg 
      aaccaaatc cggacgaag tttagcaat attatgatc 
      gaacatgcg gcccagcag cgagttggg gcgttcagt 
      gataaggat aaggtcact cttaatctt gaagaagtg 
      tacaaaacg tccaaaacc aagtgcaag aacgcgttt 
      aaaagcaca acgaggatg ttcaagcgg aatcaggat 
      tcgcttgat agtgaaaat tcggggcag tttgttaaa 
      aatgaggcg ttttttgca agagtgccg ctcacgccg 
      aatgatatt gagtataag tttaatttc aacaatgaa 
      aagggcacc aatgatgat gagaaggct gagtttgat 
      ttttcatcg attaaatcg gcgtttcgg agaagtaaa 
      ttcgtggcg gaggggcaa aatgggttt gaagatctt 
      agaagatac gtgaaacag gggggcgat ttctgcaag 
      gaactcgcc accatactc caagaaagg gccgaagct 
      gagacccaa tacgccaaa aacttatca aaactcagc 
      tcaaaacta tcccgagca tgtcgcgac ggcgtcggc 
      ggcctaaac gaagcctgg aaagccgtc gctattgaa 
      ctggaaact cgggctgaa atccaccgc ctgatgggc 
      caagccctc tcggaggag acggcgaaa ccgctccgg 
      aacatgacg gagaaccag caccggacc cggaaacag 
      tgcgaaggg acggtggac aaagcggcc aaatccttg 
      gccgaatgg aggacagct gaggccaag agcaagaag 
      caaagccat acgtgtgcc agagacaac gaaaagttg 
      caagatgcc gccgttttg gatacgtcg aggtcgggg 
      aagttgacg ttgaatagg agcagcagt ttgatccag 
      ctggcgcac atgcacaat aaaaacaca ccggataag 
      gaaaatacg aagctggaa gggaagaag cgcaaggcg 
      gaggatgca gtcaagaag gcggatatc gagtattac 
      acgctttgc gtccgggca gagagggca agactggaa 
      tgggaatcg gccgttttg cggggcgtc aacacgttt 
      cagtcgctt gaagaagag agattgaat catctgaaa 
      accgttcta acgtcgtat cttcatcac agtgccgaa 
      ctagggcct agactaata gaagcatct gagagactc 
      aaagccccg gtcacgcaa gctgaatcg ggtaaagat 
      ttgagcact tttattaat ttgagacaa tcctcgcaa 
      caagtctcg gaacagctt ttgccggat ttttactgc 
      gaacatatt actttggct atgaatagg gaacggcgg 
      aagcaggct ttggtcaaa ttactacac ctaatccgg 
      caagatatc gaaagggag aggaagtct aagaatggt 
      ttggaaaac ttatctaaa gccatcaag caaacccca 
      aattttggt gctgaggat tcgcaacaa agtgtcagc 
      gaaaaactg tatcatatg aagtcgatg ttaacgtat 
      ttggaggga gcacgatac aaactacac aatgcttta 
      gcagaactt gacagtaaa ccaaggtct gggcatccc 
      ctagcagcc catattacc ataacgagg gataaatcg 
      ggattgcaa caaagcata cttaaggtg cctcagtgg 
      ttgaaagaa gaatggttc gagacggag aagagcccc 
      ctgagtgaa aaatccgtc agatcaagc aaatcggat 
      aattccgat ccgcacaac aatgacata gccgacccc 
      gaatggacc gacaggggg gctgctgac ggcaactct 
      aatcagcct gactccgat ttcgatgag ttttcgtct 
      caatgcagc agtgctgaa aacatttgc aacacggaa 
      gagagtcca gtgcagcca atcgcacaa tgcaaagct 
      ctgtacgct tacactccc aatttaacc gacgagttg 
      gcgttacaa ccaggcgat ctcctctcg gtttatcgg 
      cagcaagac gacggctgg tggctggga gagtgcaaa 
      ggaaacgtc gggattttc cccgcaact tacgtcgag 
      ctgatcaat tcgaactaa
    mRNA sequence
    • >TC013784 RA
      cacacacac atgtgataa ttatagcac cacagacta 
      atatagtga ggtcttgct ccgcgttgg ccctgcggt 
      agttctgaa tgaagcgcg agtgcgaaa agtgaaaaa 
      tgtgaactt tcgagtgtt gtgttatgt caccgtcat 
      atgatagca agcacaata tgcgacctt cccaataat 
      ttaccttta acaatttca tcgacgtca acattttcc 
      ttccttatc taaccgtac acacaacct aattagcgt 
      ttatcctgc ttttttcac aaatgagtg tcagtaagt 
      agcggcaat tgagtgcga cgcgagcga aaatgaaaa 
      aaatctgca ataaaatat cgaaacagc agtggagaa 
      tcaccgttc cgaatatca actcgaaaa aagccgcta 
      ccgagcgaa aatcgggct cttacgact tggaagcgg 
      cccaaaagg aatacgacc gcactagaa ctgtgccaa 
      acagtgttt ttgtaaaca gtgatagtg atagtgaca 
      ccgacgacg aaaaattcc aagacgcaa tggaaaatg 
      gtacaagtt acctgaacg aaccaacgg aaccagtgg 
      aatacgacg tccccaaaa tcagcttca aatttctat 
      caaagggtg aaccccccg aaaacgatg tggtgctca 
      aaagtgaag tgtgcttcg aagtcgcaa cccccgaac 
      cctcgcccg ttaaaagtg acatcgaaa ttttgccag 
      tggtgggca tcatcaagt gcggaagtg acccaaacc 
      tggtgcttc gtgaccacg aagacgacg gtctttaca 
      aaatcccga gacaattgg ccaggtgtc gtctatcac 
      aatctttaa acgatttca aagtcgagg agttccaag 
      cctacactt cgaaccact cgagcatcg aacatgtct 
      cctgtagtc aggttattg tgcaaagtc cggacgtac 
      caccggaga atttcgact attgcaaag cccggagta 
      aattaccga tgaagttac ggaggccca cttttttga 
      gaaaaccca aaaaagcag tcgatactt ggaaccaaa 
      tccggacga agtttagca atattatga tcgaacatg 
      cggcccagc agcgagttg gggcgttca gtgataagg 
      ataaggtca ctcttaatc ttgaagaag tgtacaaaa 
      cgtccaaaa ccaagtgca agaacgcgt ttaaaagca 
      caacgagga tgttcaagc ggaatcagg attcgcttg 
      atagtgaaa attcggggc agtttgtta aaaatgagg 
      cgttttttg caagagtgc cgctcacgc cgaatgata 
      ttgagtata agtttaatt tcaacaatg aaaagggca 
      ccaatgatg atgagaagg ctgagtttg atttttcat 
      cgattaaat cggcgtttc ggagaagta aattcgtgg 
      cggaggggc aaaatgggt ttgaagatc ttagaagat 
      acgtgaaac aggggggcg atttctgca aggaactcg 
      ccaccatac tccaagaaa gggccgaag ctgagaccc 
      aatacgcca aaaacttat caaaactca gctcaaaac 
      tatcccgag catgtcgcg acggcgtcg gcggcctaa 
      acgaagcct ggaaagccg tcgctattg aactggaaa 
      ctcgggctg aaatccacc gcctgatgg gccaagccc 
      tctcggagg agacggcga aaccgctcc ggaacatga 
      cggagaacc agcaccgga cccggaaac agtgcgaag 
      ggacggtgg acaaagcgg ccaaatcct tggccgaat 
      ggaggacag ctgaggcca agagcaaga agcaaagcc 
      atacgtgtg ccagagaca acgaaaagt tgcaagatg 
      ccgccgttt tggatacgt cgaggtcgg ggaagttga 
      cgagcagca gtttgatcc agctggcgc acatgcaca 
      ataaaaaca caccggata aggaaaata cgaagctgg 
      aagggaaga agcgcaagg cggaggatg cagtcaaga 
      aggcggata tcgagtatt acacgcttt gcgtccggg 
      cagagaggg caagactgg aatgggaat cggccgttt 
      tgcggggcg tcaacacgt ttcagtcgc ttgaagaag 
      agagattga atcatctga aaaccgttc taacgtcgt 
      atcttcatc acagtgccg aactagggc ctagactaa 
      tagaagcat ctgagagac tcaaagccc cggtcacgc 
      aagctgaat cgggtaaag atttgagca cttttatta 
      atttgagac aatcctcgc aacaagtct cggaacagc 
      ttttgccgg atttttact gcgaacata ttactttgg 
      ctatgaata gggaacggc ggaagcagg ctttggtca 
      aattactac acctaatcc ggcaagata tcgaaaggg 
      agaggaagt ctaagaatg gtttggaaa acttatcta 
      aagccatca agcaaaccc caaattttg gtgctgagg 
      attcgcaac aaagtgtca gcgaaaaac tgtatcata 
      tgaagtcga tgttaacgt atttggagg gagcacgat 
      acaaactac acaatgctt tagcagaac ttgacagta 
      aaccaaggt ctgggcatc ccctagcag cccatatta 
      ccataacga gggataaat cgggattgc aacaaagca 
      tacttaagg tgcctcagt ggttgaaag aagaatggt 
      tcgagacgg agaagagcc ccctgagtg aaaaatccg 
      tcagatcaa gcaaatcgg ataattccg atccgcaca 
      acaatgaca tagccgacc ccgaatgga ccgacaggg 
      gggctgctg acggcaact ctaatcagc ctgactccg 
      atttcgatg agttttcgt ctcaatgca gcagtgctg 
      aaaacattt gcaacacgg aagagagtc cagtgcagc 
      caatcgcac aatgcaaag ctctgtacg cttacactc 
      ccaatttaa ccgacgagt tggcgttac aaccaggcg 
      atctcctct cggtttatc ggcagcaag acgacggct 
      ggtggctgg gagagtgca aaggaaacg tcgggattt 
      tccccgcaa cttacgtcg agctgatca attcgaact 
      aaagactgg agaacgatt ccacgttgg gataaacgc 
      tgtacaaat aattttaac gttcttccc tccccactt 
      tatacaaat tttttattc acaacttat taatatttt 
      gttaacagc agtgataat aaaagtgta actcctcc
    • >TC013784 RB
      cacacacac atgtgataa ttatagcac cacagacta 
      atatagtga ggtcttgct ccgcgttgg ccctgcggt 
      agttctgaa tgaagcgcg agtgcgaaa agtgaaaaa 
      tgtgaactt tcgagtgtt gtgttatgt caccgtcat 
      atgatagca agcacaata tgcgacctt cccaataat 
      ttaccttta acaatttca tcgacgtca acattttcc 
      ttccttatc taaccgtac acacaacct aattagcgt 
      ttatcctgc ttttttcac aaatgagtg tcagtaagt 
      agcggcaat tgagtgcga cgcgagcga aaatgaaaa 
      aaatctgca ataaaatat cgaaacagc agtggagaa 
      tcaccgttc cgaatatca actcgaaaa aagccgcta 
      ccgagcgaa aatcgggct cttacgact tggaagcgg 
      cccaaaagg aatacgacc gcactagaa ctgtgccaa 
      acagtgttt ttgtaaaca gtgatagtg atagtgaca 
      ccgacgacg aaaaattcc aagacgcaa tggaaaatg 
      gtacaagtt acctgaacg aaccaacgg aaccagtgg 
      aatacgacg tccccaaaa tcagcttca aatttctat 
      caaagggtg aaccccccg aaaacgatg tggtgctca 
      aaagtgaag tgtgcttcg aagtcgcaa cccccgaac 
      cctcgcccg ttaaaagtg acatcgaaa ttttgccag 
      tggtgggca tcatcaagt gcggaagtg acccaaacc 
      tggtgcttc gtgaccacg aagacgacg gtctttaca 
      aaatcccga gacaattgg ccaggtgtc gtctatcac 
      aatctttaa acgatttca aagtcgagg agttccaag 
      cctacactt cgaaccact cgagcatcg aacatgtct 
      cctgtagtc aggttattg tgcaaagtc cggacgtac 
      caccggaga atttcgact attgcaaag cccggagta 
      aattaccga tgaagttac ggaggccca cttttttga 
      gaaaaccca aaaaagcag tcgatactt ggaaccaaa 
      tccggacga agtttagca atattatga tcgaacatg 
      cggcccagc agcgagttg gggcgttca gtgataagg 
      ataaggtca ctcttaatc ttgaagaag tgtacaaaa 
      cgtccaaaa ccaagtgca agaacgcgt ttaaaagca 
      caacgagga tgttcaagc ggaatcagg attcgcttg 
      atagtgaaa attcggggc agtttgtta aaaatgagg 
      cgttttttg caagagtgc cgctcacgc cgaatgata 
      ttgagtata agtttaatt tcaacaatg aaaagggca 
      ccaatgatg atgagaagg ctgagtttg atttttcat 
      cgattaaat cggcgtttc ggagaagta aattcgtgg 
      cggaggggc aaaatgggt ttgaagatc ttagaagat 
      acgtgaaac aggggggcg atttctgca aggaactcg 
      ccaccatac tccaagaaa gggccgaag ctgagaccc 
      aatacgcca aaaacttat caaaactca gctcaaaac 
      tatcccgag catgtcgcg acggcgtcg gcggcctaa 
      acgaagcct ggaaagccg tcgctattg aactggaaa 
      ctcgggctg aaatccacc gcctgatgg gccaagccc 
      tctcggagg agacggcga aaccgctcc ggaacatga 
      cggagaacc agcaccgga cccggaaac agtgcgaag 
      ggacggtgg acaaagcgg ccaaatcct tggccgaat 
      ggaggacag ctgaggcca agagcaaga agcaaagcc 
      atacgtgtg ccagagaca acgaaaagt tgcaagatg 
      ccgccgttt tggatacgt cgaggtcgg ggaagttga 
      cgttgaata ggagcagca gtttgatcc agctggcgc 
      acatgcaca ataaaaaca caccggata aggaaaata 
      cgaagctgg aagggaaga agcgcaagg cggaggatg 
      cagtcaaga aggcggata tcgagtatt acacgcttt 
      gcgtccggg cagagaggg caagactgg aatgggaat 
      cggccgttt tgcggggcg tcaacacgt ttcagtcgc 
      ttgaagaag agagattga atcatctga aaaccgttc 
      taacgtcgt atcttcatc acagtgccg aactagggc 
      ctagactaa tagaagcat ctgagagac tcaaagccc 
      cggtcacgc aagctgaat cgggtaaag atttgagca 
      cttttatta atttgagac aatcctcgc aacaagtct 
      cggaacagc ttttgccgg atttttact gcgaacata 
      ttactttgg ctatgaata gggaacggc ggaagcagg 
      ctttggtca aattactac acctaatcc ggcaagata 
      tcgaaaggg agaggaagt ctaagaatg gtttggaaa 
      acttatcta aagccatca agcaaaccc caaattttg 
      gtgctgagg attcgcaac aaagtgtca gcgaaaaac 
      tgtatcata tgaagtcga tgttaacgt atttggagg 
      gagcacgat acaaactac acaatgctt tagcagaac 
      ttgacagta aaccaaggt ctgggcatc ccctagcag 
      cccatatta ccataacga gggataaat cgggattgc 
      aacaaagca tacttaagg tgcctcagt ggttgaaag 
      aagaatggt tcgagacgg agaagagcc ccctgagtg 
      aaaaatccg tcagatcaa gcaaatcgg ataattccg 
      atccgcaca acaatgaca tagccgacc ccgaatgga 
      ccgacaggg gggctgctg acggcaact ctaatcagc 
      ctgactccg atttcgatg agttttcgt ctcaatgca 
      gcagtgctg aaaacattt gcaacacgg aagagagtc 
      cagtgcagc caatcgcac aatgcaaag ctctgtacg 
      cttacactc ccaatttaa ccgacgagt tggcgttac 
      aaccaggcg atctcctct cggtttatc ggcagcaag 
      acgacggct ggtggctgg gagagtgca aaggaaacg 
      tcgggattt tccccgcaa cttacgtcg agctgatca 
      attcgaact aaagactgg agaacgatt ccacgttgg 
      gataaacgc tgtacaaat aattttaac gttcttccc 
      tccccactt tatacaaat tttttattc acaacttat 
      taatatttt gttaacagc agtgataat aaaagtgta 
      actcctcc