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Gene information
General information
TC013782 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 3170904..3178271
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013782
    iBeetle screen iB_02204
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013782 RA
      MEEILAPLR ASVKEQGDL VRKLKAEGA PVLDVKKAV 
      TELNARKKI LEEKELSLA PQVATFDRA KMEDLLKRR 
      FFYDQSFAI YGGITGQFD FGPMGCAFK ANLLQAWRQ 
      FFILEEQML EVDCSILTP EPVLKASGH VDRFADLMV 
      KDVGTGECF RLDHLIKAH LEKISADKR TTDDVKSDC 
      QDIVVKLDG MTKAEMAAV MQKYNMKSP LTGNDLTEP 
      IEFNLMFGT QIGPSGLIK GFLRPETAQ GIFVNFKRL 
      LEFNQGRLP FAAAQIGNA FRNEISPRS GLIRVREFT 
      MAEIEHFCD PADKNHPKF DSIRDTELL LYSACNQMD 
      GKPAEKLTI GSAVDKGLV ANATLGYFI ARIHKFLVK 
      CGVDEKRLR FRQHMANEM AHYAQDCWD AECLTSYGW 
      IECVGCADR SAFDLTQHT QATGVRLAA EKKLAEPKI 
      VDVVEATPN KGVLGKTFK KDAKIITDA LTQLQVSEV 
      EALEKDLES NGSHELTID DKKFTLTKE MVTVKRYQK 
      TLHVEEIIP SVIEPSFGV GRITYAIFE HNFKMREND 
      EQRTYLSLP ASIAPLKCS VLPLSSNAE FAPFVKQLS 
      TALTKVDVS HKIDDSSGS IGRRYARTD EIAIPYGIT 
      IDFDTLKEP HSVTLRERD SMAQVRIPL DDIATVLHD 
      LSYSKKSWA DIEGKYPKF EQQESKGV
    • >TC013782 RB
      MESQSECYF NIPVLLIST IVSFLSVHS TIIEKPVFK 
      WLNKQTGKE RELQLQKLA LKNEQSSLS MVDEFAKYS 
      KLQRRINVI DRELEQIKG DKPTNSFVV QLGFTNCVT 
      ARPLCISSV VSSKKWGIN KKHRNVKLQ LYFDSMADP 
      QMEEILAPL RASVKEQGD LVRKLKAEG APVLDVKKA 
      VTELNARKK ILEEKELSL APQVATFDR AKMEDLLKR 
      RFFYDQSFA IYGGITGQF DFGPMGCAF KANLLQAWR 
      QFFILEEQM LEVDCSILT PEPVLKASG HVDRFADLM 
      VKDVGTGEC FRLDHLIKA HLEKISADK RTTDDVKSD 
      CQDIVVKLD GMTKAEMAA VMQKYNMKS PLTGNDLTE 
      PIEFNLMFG TQIGPSGLI KGFLRPETA QGIFVNFKR 
      LLEFNQGRL PFAAAQIGN AFRNEISPR SGLIRVREF 
      TMAEIEHFC DPADKNHPK FDSIRDTEL LLYSACNQM 
      DGKPAEKLT IGSAVDKGL VANATLGYF IARIHKFLV 
      KCGVDEKRL RFRQHMANE MAHYAQDCW DAECLTSYG 
      WIECVGCAD RSAFDLTQH TQATGVRLA AEKKLAEPK 
      IVDVVEATP NKGVLGKTF KKDAKIITD ALTQLQVSE 
      VEALEKDLE SNGSHELTI DDKKFTLTK EMVTVKRYQ 
      KTLHVEEII PSVIEPSFG VGRITYAIF EHNFKMREN 
      DEQRTYLSL PASIAPLKC SVLPLSSNA EFAPFVKQL 
      STALTKVDV SHKIDDSSG SIGRRYART DEIAIPYGI 
      TIDFDTLKE PHSVTLRER DSMAQVRIP LDDIATVLH 
      DLSYSKKSW ADIEGKYPK FEQQESKGV
    • >TC013782 RC
      MVDEFAKYS KLQRRINVI DRELEQIKG DKPTNSFVV 
      QLGFTYGVK LVFALLLIL LSLYYRYTP VLYLGDKIS 
      LTPFTNFIC YPNDANYVS FYFWAMCCV TVARLI
    CDS sequence
    • >TC013782 RA
      atggaggaa attttggcc cccttgagg gccagtgtc 
      aaggaacag ggcgatttg gttcgcaaa ttgaaggcg 
      gaaggggcc cccgttttg gacgtgaaa aaggccgtt 
      acggagctg aacgctcga aaaaaaatc ctagaggag 
      aaagagctg agtttggcc ccacaagtt gccactttt 
      gacagggct aaaatggag gatttgttg aaaaggcgg 
      tttttttac gaccagtcg tttgcgatt tatggggga 
      atcaccggt cagtttgat tttgggcca atggggtgc 
      gcgttcaag gccaactta ctgcaagcc tggagacag 
      tttttcata ttggaggag caaatgttg gaggttgat 
      tgttcaatt ctcactcct gagcccgtt ttaaaagcg 
      tccggacat gttgacaga tttgcggat cttatggtt 
      aaagacgtg gggactggc gagtgtttt aggttggat 
      catttgata aaagcccat ttggagaaa atctcagct 
      gataagagg accacagat gatgtcaag tcagattgc 
      caggacatt gttgtaaag ttggatgga atgacgaaa 
      gcggaaatg gctgctgtg atgcagaaa tacaacatg 
      aagagccct ctgacgggg aatgatctc acagaaccc 
      attgagttt aatctaatg tttggaacg caaatcgga 
      ccttcggga cttatcaaa gggtttttg cgtccagag 
      acggcacaa ggcattttt gttaacttc aaacgactt 
      ttggagttc aaccaaggg cgtttgccc tttgctgcc 
      gctcaaatc ggaaacgct tttcgcaac gaaatttcc 
      ccccgttct ggtttgatt cgcgtccgt gaatttaca 
      atggccgaa atcgagcat ttttgcgac cctgctgat 
      aaaaatcac ccgaaattt gatagcatt cgggacact 
      gaactatta ttgtactcc gcttgtaac caaatggac 
      ggaaaaccg gcggaaaaa ctgacaatt ggctcagct 
      gtggataag ggattggtg gctaacgcg actttgggg 
      tattttatc gcacgtatc cacaaattt ttggttaaa 
      tgtggcgtt gatgagaaa cggttgcga tttaggcag 
      catatggcc aacgaaatg gcccattat gcgcaagat 
      tgctgggat gccgagtgt ctcacaagc tatggttgg 
      atagagtgt gtgggttgt gccgatcgg tctgccttt 
      gatttaaca caacacact caagctaca ggagttcga 
      ttagcggcg gaaaaaaaa ttggctgag ccgaaaatt 
      gtcgatgtt gttgaggcg acccctaat aagggggtt 
      ttaggaaaa actttcaag aaggatgcg aaaatcatc 
      acggatgcg ctcacacaa ttacaagtt tcggaagtt 
      gaagctctt gaaaaggat ttagagagt aatggatca 
      cacgaatta acaattgat gataaaaag ttcactctg 
      acgaaagaa atggtaact gtaaaacgt taccaaaag 
      acgctccat gtggaagaa atcattcct agtgttatt 
      gaaccatcg tttggtgtg ggacgaata acgtacgca 
      atcttcgaa cacaatttc aaaatgcgt gagaatgac 
      gaacagcgg acttatctt tcactgccc gcttcaata 
      gcaccgtta aagtgtagt gtacttccc ctaagctct 
      aatgcggaa tttgcaccg tttgttaaa caactatct 
      actgcccta acaaaagtc gacgtttca cacaaaata 
      gacgacagt tctggttct ataggacgc agatatgcc 
      agaactgat gaaatcgct ataccatat ggtatcaca 
      attgacttt gatacactc aaagaacca cacagtgtg 
      actttgaga gagcgcgat tctatggcg caagtcaga 
      attccgctg gacgatatt gcgacagtt ttgcacgat 
      ttgtcgtac agtaaaaaa tcatgggcc gatattgag 
      ggcaaatac ccgaagttc gagcaacag gaaagcaaa 
      ggagtttga
    • >TC013782 RB
      atggagtca caatctgag tgttacttc aacattcct 
      gttttgtta atttcgacg attgttagt tttttaagt 
      gtccattca acaataatc gaaaaaccc gtttttaag 
      tggctgaat aagcaaaca gggaaagaa agggagctg 
      cagctccag aaacttgcg ttaaaaaat gagcagtca 
      tctctcagt atggtggac gagtttgca aaatactca 
      aagcttcaa agacgcatc aatgtgatt gatcgagaa 
      ctagaacag ataaaaggg gacaaacca accaacagt 
      tttgtcgta cagttgggt ttcacaaat tgtgtcacc 
      gctcgacca ctttgcatc agttcagtt gtctcgtcg 
      aaaaagtgg ggcattaac aagaagcac cgaaacgta 
      aaattgcag ttgtatttt gactcgatg gcggacccc 
      cagatggag gaaattttg gcccccttg agggccagt 
      gtcaaggaa cagggcgat ttggttcgc aaattgaag 
      gcggaaggg gcccccgtt ttggacgtg aaaaaggcc 
      gttacggag ctgaacgct cgaaaaaaa atcctagag 
      gagaaagag ctgagtttg gccccacaa gttgccact 
      tttgacagg gctaaaatg gaggatttg ttgaaaagg 
      cggtttttt tacgaccag tcgtttgcg atttatggg 
      ggaatcacc ggtcagttt gattttggg ccaatgggg 
      tgcgcgttc aaggccaac ttactgcaa gcctggaga 
      cagtttttc atattggag gagcaaatg ttggaggtt 
      gattgttca attctcact cctgagccc gttttaaaa 
      gcgtccgga catgttgac agatttgcg gatcttatg 
      gttaaagac gtggggact ggcgagtgt tttaggttg 
      gatcatttg ataaaagcc catttggag aaaatctca 
      gctgataag aggaccaca gatgatgtc aagtcagat 
      tgccaggac attgttgta aagttggat ggaatgacg 
      aaagcggaa atggctgct gtgatgcag aaatacaac 
      atgaagagc cctctgacg gggaatgat ctcacagaa 
      cccattgag tttaatcta atgtttgga acgcaaatc 
      ggaccttcg ggacttatc aaagggttt ttgcgtcca 
      gagacggca caaggcatt tttgttaac ttcaaacga 
      cttttggag ttcaaccaa gggcgtttg ccctttgct 
      gccgctcaa atcggaaac gcttttcgc aacgaaatt 
      tccccccgt tctggtttg attcgcgtc cgtgaattt 
      acaatggcc gaaatcgag catttttgc gaccctgct 
      gataaaaat cacccgaaa tttgatagc attcgggac 
      actgaacta ttattgtac tccgcttgt aaccaaatg 
      gacggaaaa ccggcggaa aaactgaca attggctca 
      gctgtggat aagggattg gtggctaac gcgactttg 
      gggtatttt atcgcacgt atccacaaa tttttggtt 
      aaatgtggc gttgatgag aaacggttg cgatttagg 
      cagcatatg gccaacgaa atggcccat tatgcgcaa 
      gattgctgg gatgccgag tgtctcaca agctatggt 
      tggatagag tgtgtgggt tgtgccgat cggtctgcc 
      tttgattta acacaacac actcaagct acaggagtt 
      cgattagcg gcggaaaaa aaattggct gagccgaaa 
      attgtcgat gttgttgag gcgacccct aataagggg 
      gttttagga aaaactttc aagaaggat gcgaaaatc 
      atcacggat gcgctcaca caattacaa gtttcggaa 
      gttgaagct cttgaaaag gatttagag agtaatgga 
      tcacacgaa ttaacaatt gatgataaa aagttcact 
      ctgacgaaa gaaatggta actgtaaaa cgttaccaa 
      aagacgctc catgtggaa gaaatcatt cctagtgtt 
      attgaacca tcgtttggt gtgggacga ataacgtac 
      gcaatcttc gaacacaat ttcaaaatg cgtgagaat 
      gacgaacag cggacttat ctttcactg cccgcttca 
      atagcaccg ttaaagtgt agtgtactt cccctaagc 
      tctaatgcg gaatttgca ccgtttgtt aaacaacta 
      tctactgcc ctaacaaaa gtcgacgtt tcacacaaa 
      atagacgac agttctggt tctatagga cgcagatat 
      gccagaact gatgaaatc gctatacca tatggtatc 
      acaattgac tttgataca ctcaaagaa ccacacagt 
      gtgactttg agagagcgc gattctatg gcgcaagtc 
      agaattccg ctggacgat attgcgaca gttttgcac 
      gatttgtcg tacagtaaa aaatcatgg gccgatatt 
      gagggcaaa tacccgaag ttcgagcaa caggaaagc 
      aaaggagtt tga
    • >TC013782 RC
      atggtggac gagtttgca aaatactca aagcttcaa 
      agacgcatc aatgtgatt gatcgagaa ctagaacag 
      ataaaaggg gacaaacca accaacagt tttgtcgta 
      cagttgggt ttcacgtac ggtgtcaag ttggtcttt 
      gccttgttg ttaatactg ttatcgctt tattaccgg 
      tacactcct gtgctatat ctaggcgat aaaatcagt 
      ttaacgccg tttacgaac tttatttgc tatccaaat 
      gacgccaat tatgtgtct ttttacttt tgggcgatg 
      tgttgcgtg actgtggcc cgattaatt taa
    mRNA sequence
    • >TC013782 RA
      aaattgtgt caccgctcg accactttg catcagttc 
      agttgtctc gtcgaaaaa gtggggcat taacaagaa 
      gcaccgaaa cgtaaaatt gcagttgta ttttgactc 
      gatggcgga cccccagat ggaggaaat tttggcccc 
      cttgagggc cagtgtcaa ggaacaggg cgatttggt 
      tcgcaaatt gaaggcgga aggggcccc cgttttgga 
      cgtgaaaaa ggccgttac ggagctgaa cgctcgaaa 
      aaaaatcct agaggagaa agagctgag tttggcccc 
      acaagttgc cacttttga cagggctaa aatggagga 
      tttgttgaa aaggcggtt tttttacga ccagtcgtt 
      tgcgattta tgggggaat caccggtca gtttgattt 
      tgggccaat ggggtgcgc gttcaaggc caacttact 
      gcaagcctg gagacagtt tttcatatt ggaggagca 
      aatgttgga ggttgattg ttcaattct cactcctga 
      gcccgtttt aaaagcgtc cggacatgt tgacagatt 
      tgcggatct tatggttaa agacgtggg gactggcga 
      gtgttttag gttggatca tttgataaa agcccattt 
      ggagaaaat ctcagctga taagaggac cacagatga 
      tgtcaagtc agattgcca ggacattgt tgtaaagtt 
      ggatggaat gacgaaagc ggaaatggc tgctgtgat 
      gcagaaata caacatgaa gagccctct gacggggaa 
      tgatctcac agaacccat tgagtttaa tctaatgtt 
      tggaacgca aatcggacc ttcgggact tatcaaagg 
      gtttttgcg tccagagac ggcacaagg catttttgt 
      taacttcaa acgactttt ggagttcaa ccaagggcg 
      tttgccctt tgctgccgc tcaaatcgg aaacgcttt 
      tcgcaacga aatttcccc ccgttctgg tttgattcg 
      cgtccgtga atttacaat ggccgaaat cgagcattt 
      ttgcgaccc tgctgataa aaatcaccc gaaatttga 
      tagcattcg ggacactga actattatt gtactccgc 
      ttgtaacca aatggacgg aaaaccggc ggaaaaact 
      gacaattgg ctcagctgt ggataaggg attggtggc 
      taacgcgac tttggggta ttttatcgc acgtatcca 
      caaattttt ggttaaatg tggcgttga tgagaaacg 
      gttgcgatt taggcagca tatggccaa cgaaatggc 
      ccattatgc gcaagattg ctgggatgc cgagtgtct 
      cacaagcta tggttggat agagtgtgt gggttgtgc 
      cgatcggtc tgcctttga tttaacaca acacactca 
      agctacagg agttcgatt agcggcgga aaaaaaatt 
      ggctgagcc gaaaattgt cgatgttgt tgaggcgac 
      ccctaataa gggggtttt aggaaaaac tttcaagaa 
      ggatgcgaa aatcatcac ggatgcgct cacacaatt 
      acaagtttc ggaagttga agctcttga aaaggattt 
      agagagtaa tggatcaca cgaattaac aattgatga 
      taaaaagtt cactctgac gaaagaaat ggtaactgt 
      aaaacgtta ccaaaagac gctccatgt ggaagaaat 
      cattcctag tgttattga accatcgtt tggtgtggg 
      acgaataac gtacgcaat cttcgaaca caatttcaa 
      aatgcgtga gaatgacga acagcggac ttatctttc 
      actgcccgc ttcaatagc accgttaaa gtgtagtgt 
      acttcccct aagctctaa tgcggaatt tgcaccgtt 
      tgttaaaca actatctac tgccctaac aaaagtcga 
      cgtttcaca caaaataga cgacagttc tggttctat 
      aggacgcag atatgccag aactgatga aatcgctat 
      accatatgg tatcacaat tgactttga tacactcaa 
      agaaccaca cagtgtgac tttgagaga gcgcgattc 
      tatggcgca agtcagaat tccgctgga cgatattgc 
      gacagtttt gcacgattt gtcgtacag taaaaaatc 
      atgggccga tattgaggg caaataccc gaagttcga 
      gcaacagga aagcaaagg agtttgagg gaattgtcc 
      agttttaat ttattataa ttataagct tgaatgaaa 
      ttaaa
    • >TC013782 RB
      aactaaaga caccagaac actagtttt tcatgtcag 
      gtttttatt tccttgtaa acatttaaa aaacactaa 
      taattgttt aattaatgt aaaatgtaa accttaaca 
      ctacttttc cacctccgt ttaaaccaa ttgtcaagt 
      catggagtc acaatctga gtgttactt caacattcc 
      tgttttgtt aatttcgac gattgttag ttttttaag 
      tgtccattc aacaataat cgaaaaacc cgtttttaa 
      gtggctgaa taagcaaac agggaaaga aagggagct 
      gcagctcca gaaacttgc gttaaaaaa tgagcagtc 
      atctctcag tatggtgga cgagtttgc aaaatactc 
      aaagcttca aagacgcat caatgtgat tgatcgaga 
      actagaaca gataaaagg ggacaaacc aaccaacag 
      ttttgtcgt acagttggg tttcacaaa ttgtgtcac 
      cgctcgacc actttgcat cagttcagt tgtctcgtc 
      gaaaaagtg gggcattaa caagaagca ccgaaacgt 
      aaaattgca gttgtattt tgactcgat ggcggaccc 
      ccagatgga ggaaatttt ggccccctt gagggccag 
      tgtcaagga acagggcga tttggttcg caaattgaa 
      ggcggaagg ggcccccgt tttggacgt gaaaaaggc 
      cgttacgga gctgaacgc tcgaaaaaa aatcctaga 
      ggagaaaga gctgagttt ggccccaca agttgccac 
      ttttgacag ggctaaaat ggaggattt gttgaaaag 
      gcggttttt ttacgacca gtcgtttgc gatttatgg 
      gggaatcac cggtcagtt tgattttgg gccaatggg 
      gtgcgcgtt caaggccaa cttactgca agcctggag 
      acagttttt catattgga ggagcaaat gttggaggt 
      tgattgttc aattctcac tcctgagcc cgttttaaa 
      agcgtccgg acatgttga cagatttgc ggatcttat 
      ggttaaaga cgtggggac tggcgagtg ttttaggtt 
      ggatcattt gataaaagc ccatttgga gaaaatctc 
      agctgataa gaggaccac agatgatgt caagtcaga 
      ttgccagga cattgttgt aaagttgga tggaatgac 
      gaaagcgga aatggctgc tgtgatgca gaaatacaa 
      catgaagag ccctctgac ggggaatga tctcacaga 
      acccattga gtttaatct aatgtttgg aacgcaaat 
      cggaccttc gggacttat caaagggtt tttgcgtcc 
      agagacggc acaaggcat ttttgttaa cttcaaacg 
      acttttgga gttcaacca agggcgttt gccctttgc 
      tgccgctca aatcggaaa cgcttttcg caacgaaat 
      ttccccccg ttctggttt gattcgcgt ccgtgaatt 
      tacaatggc cgaaatcga gcatttttg cgaccctgc 
      tgataaaaa tcacccgaa atttgatag cattcggga 
      cactgaact attattgta ctccgcttg taaccaaat 
      ggacggaaa accggcgga aaaactgac aattggctc 
      agctgtgga taagggatt ggtggctaa cgcgacttt 
      ggggtattt tatcgcacg tatccacaa atttttggt 
      taaatgtgg cgttgatga gaaacggtt gcgatttag 
      gcagcatat ggccaacga aatggccca ttatgcgca 
      agattgctg ggatgccga gtgtctcac aagctatgg 
      ttggataga gtgtgtggg ttgtgccga tcggtctgc 
      ctttgattt aacacaaca cactcaagc tacaggagt 
      tcgattagc ggcggaaaa aaaattggc tgagccgaa 
      aattgtcga tgttgttga ggcgacccc taataaggg 
      ggttttagg aaaaacttt caagaagga tgcgaaaat 
      catcacgga tgcgctcac acaattaca agtttcgga 
      agttgaagc tcttgaaaa ggatttaga gagtaatgg 
      atcacacga attaacaat tgatgataa aaagttcac 
      tctgacgaa agaaatggt aactgtaaa acgttacca 
      aaagacgct ccatgtgga agaaatcat tcctagtgt 
      tattgaacc atcgtttgg tgtgggacg aataacgta 
      cgcaatctt cgaacacaa tttcaaaat gcgtgagaa 
      tgacgaaca gcggactta tctttcact gcccgcttc 
      aatagcacc gttaaagtg tagtgtact tcccctaag 
      ctctaatgc ggaatttgc accgtttgt taaacaact 
      atctactgc cctaacaaa agtcgacgt ttcacacaa 
      aatagacga cagttctgg ttctatagg acgcagata 
      tgccagaac tgatgaaat cgctatacc atatggtat 
      cacaattga ctttgatac actcaaaga accacacag 
      tgtgacttt gagagagcg cgattctat ggcgcaagt 
      cagaattcc gctggacga tattgcgac agttttgca 
      cgatttgtc gtacagtaa aaaatcatg ggccgatat 
      tgagggcaa atacccgaa gttcgagca acaggaaag 
      caaaggagt ttgagggaa ttgtccagt tttaattta 
      ttataatta taagcttga atgaaatta aa
    • >TC013782 RC
      aactaaaga caccagaac actagtttt tcatgtcag 
      gtttttatt tccttgtaa acatttaaa aaacactaa 
      taattgttt aattaatgt aaaatgtaa accttaaca 
      ctacttttc cacctccgt ttaaaccaa ttgtcaagt 
      catggagtc acaatctga gtgttactt caacattcc 
      tgttttgtt aatttcgac gattgttag ttttttaag 
      tgtccattc aacaataat cgaaaaacc cgtttttaa 
      gtggctgaa taagcaaac agggaaaga aagggagct 
      gcagctcca gaaacttgc gttaaaaaa tgagcagtc 
      atctctcag tatggtgga cgagtttgc aaaatactc 
      aaagcttca aagacgcat caatgtgat tgatcgaga 
      actagaaca gataaaagg ggacaaacc aaccaacag 
      ttttgtcgt acagttggg tttcacgta cggtgtcaa 
      gttggtctt tgccttgtt gttaatact gttatcgct 
      ttattaccg gtacactcc tgtgctata tctaggcga 
      taaaatcag tttaacgcc gtttacgaa ctttatttg 
      ctatccaaa tgacgccaa ttatgtgtc tttttactt 
      ttgggcgat gtgttgcgt gactgtggc ccgattaat 
      ttaataaat tgtctcaaa attgagtgt ttttgagtt 
      attttgaaa accccgctc gtgaacagt accaatcac 
      ccaccaaat aaaccgaaa ttcccagcc ggcacatgt 
      gctcattcc tttcacttt tgcactaat taaaaaaag 
      tctgcctcg tggcagttt tctaccaat ccagtcaat 
      gaaattctc ccatatttc cc