Status
Please wait ...
Gene information
General information
TC013765 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4623388..4626700
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013765
    iBeetle screen iB_02200

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013765 RA
      MSVMGEPNF IAWAAQRSG YGGGNLTVV DKVLPDMLH 
      LVDAHWYQF PPMNPLWHG ILGFVIGVL GFVSIVGNG 
      MVIYIFSST KALRTPSNL LVVNLAFSD FLMMLCMSP 
      AMVINCYNE TWVLGPLVC ELYGMSGSL FGCASIWTM 
      TFIALDRYN VIVKGLSAQ PLTKKGAML RILIIWVFS 
      TLWTIAPFF GWNRYVPEG NMTACGTDY LTKDWVSRS 
      YILVYAVWV YFVPLFTII YSYWFIVQA VAAHEKSMR 
      EQAKKMNVA SLRSSEAAQ TSAECKLAK IALMTITLW 
      FFAWTPYLV TNFTGIFEG AKISPLATI WCSLFAKAN 
      AVYNPIVYG ISHPKYRQA LQKKFPSLV CAGEPDDTT 
      STASGVTNV TTDEKPATA
    CDS sequence
    • >TC013765 RA
      atgtctgtg atgggcgag ccaaacttc atcgcctgg 
      gcggcgcag cgctcgggc tatggcggc ggcaacctc 
      accgtcgtg gacaaagtc ctccccgac atgctccac 
      ctggtggac gcccattgg taccagttc ccgcccatg 
      aaccccctc tggcacggc attctgggc ttcgtgatc 
      ggcgttctg ggcttcgtc tccatcgtg ggcaacgga 
      atggtcatc tacatcttc tcctccacc aaggcgctg 
      cgaaccccc tcgaacctc ctcgtggtc aatttggcc 
      ttctccgac ttcctgatg atgctctgc atgtccccg 
      gctatggtc attaattgc tacaacgag acctgggtg 
      ctggggccc ctcgtgtgc gagctctac ggcatgagt 
      gggtctctc ttcggatgc gcatccatc tggaccatg 
      acgttcatc gccttggat cggtacaat gtgattgtg 
      aaggggctc agtgcgcaa ccgttgacc aagaaaggg 
      gccatgttg aggatactg atcatctgg gtcttctcc 
      acgctgtgg accatcgca cccttcttt ggatggaac 
      cgctacgtg ccagaaggc aacatgacg gcgtgtgga 
      acggattat ttgaccaag gattgggtt agtcggagc 
      tatattttg gtttacgcc gtttgggtc tatttcgtt 
      cctctcttc accatcatc tattcctac tggtttata 
      gtgcaggcc gtggccgcc cacgaaaaa tcgatgaga 
      gaacaagca aagaaaatg aacgttgcg tccttgcga 
      tcctccgaa gcagcccaa accagcgcc gaatgcaaa 
      ctcgccaag atagcccta atgaccatc acgctctgg 
      ttcttcgct tggactcct tacctggtc accaacttc 
      accggtatt ttcgaaggt gccaaaatc agtcctttg 
      gccaccatc tggtgctcg ctctttgct aaagccaac 
      gctgtctac aacccgatc gtatacgga atcagccat 
      ccaaagtac agacaagcc ctccagaag aagttcccg 
      tcgttggtg tgcgcgggc gagccggac gacaccacc 
      tccacggct tcgggggta acgaacgtt accaccgac 
      gagaagccg gccacggct taa
    mRNA sequence
    • >TC013765 RA
      tgtcgtgaa aggaagtcg caacggacg caacttccg 
      ttggatgtg ttctcttac cattggaat acctccaag 
      aagccgtga tttaaggcc accatgtct gtgatgggc 
      gagccaaac ttcatcgcc tgggcggcg cagcgctcg 
      ggctatggc ggcggcaac ctcaccgtc gtggacaaa 
      gtcctcccc gacatgctc cacctggtg gacgcccat 
      tggtaccag ttcccgccc atgaacccc ctctggcac 
      ggcattctg ggcttcgtg atcggcgtt ctgggcttc 
      gtctccatc gtgggcaac ggaatggtc atctacatc 
      ttctcctcc accaaggcg ctgcgaacc ccctcgaac 
      ctcctcgtg gtcaatttg gccttctcc gacttcctg 
      atgatgctc tgcatgtcc ccggctatg gtcattaat 
      tgctacaac gagacctgg gtgctgggg cccctcgtg 
      tgcgagctc tacggcatg agtgggtct ctcttcgga 
      tgcgcatcc atctggacc atgacgttc atcgccttg 
      gatcggtac aatgtgatt gtgaagggg ctcagtgcg 
      caaccgttg accaagaaa ggggccatg ttgaggata 
      ctgatcatc tgggtcttc tccacgctg tggaccatc 
      gcacccttc tttggatgg aaccgctac gtgccagaa 
      ggcaacatg acggcgtgt ggaacggat tatttgacc 
      aaggattgg gttagtcgg agctatatt ttggtttac 
      gccgtttgg gtctatttc gttcctctc ttcaccatc 
      atctattcc tactggttt atagtgcag gccgtggcc 
      gcccacgaa aaatcgatg agagaacaa gcaaagaaa 
      atgaacgtt gcgtccttg cgatcctcc gaagcagcc 
      caaaccagc gccgaatgc aaactcgcc aagatagcc 
      ctaatgacc atcacgctc tggttcttc gcttggact 
      ccttacctg gtcaccaac ttcaccggt attttcgaa 
      ggtgccaaa atcagtcct ttggccacc atctggtgc 
      tcgctcttt gctaaagcc aacgctgtc tacaacccg 
      atcgtatac ggaatcagc catccaaag tacagacaa 
      gccctccag aagaagttc ccgtcgttg gtgtgcgcg 
      ggcgagccg gacgacacc acctccacg gcttcgggg 
      gtaacgaac gttaccacc gacgagaag ccggccacg 
      gcttaagta gtgatttaa tggcttttg tttgattct 
      tgtaatgat caataagag acttcaaac tgggttttc 
      atttgtttt aattcggtg cgtggcgaa aataatcat 
      tgatgcttt gagttttca aagtgttgc gggttttgg 
      aatacaagc ttagcacaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY