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Gene information
General information
TC013758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 3718957..3733908
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013758
    iBeetle screen iB_02199
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013758 RA
      MGDVEVHSN FTITEKKTK KVKKTTKRR ESSDHGGEV 
      TITEMERTN GTQGGEYTK AMNKDWHGQ HFCCWQCDE 
      SLTGQRYVL RDEHPYCVS CYESVFANA CEKCSRIIG 
      IDSKDLSYK DKHWHEACF LCTTCGESL VDRQFGSKG 
      DRIYCGRCY DEQFASRCD GCHEIFRAG TLSDQFKSR 
      LNLQTKTVG EERIKKAKE RNEDVAILS MELPGEKKA 
      FKCCLDQDC LLGDPTSLP LGTKKMEYK TRQWHEKCF 
      CCCVCKVPI GTQSFIPRE QEIYCAKCY EEKFATRCI 
      KCNKVITSG GVTYKNEPW HRECFTCTH CSKSLAGER 
      FTSRDEKPY CAECFGELF AKRCFACNK PITGIGGTK 
      FISFEDRHW HNDCFFCAS CRTSLVGRG FITDQEDII 
      CPECAKQKL M
    CDS sequence
    • >TC013758 RA
      atgggagac gttgaagtt cattccaat tttaccatc 
      accgaaaag aaaaccaaa aaagtcaag aagaccacc 
      aaacgaagg gagagtagt gaccatggg ggcgaagtc 
      accatcact gaaatggag cgcaccaac ggcacgcag 
      ggtggcgag tacacaaag gccatgaac aaggactgg 
      catgggcaa cacttttgc tgttggcag tgcgacgag 
      agtttaact ggtcaacgc tacgtcctc cgtgacgag 
      catccctat tgcgtgtct tgttacgaa tctgtcttc 
      gccaatgct tgcgaaaag tgcagcaga atcatcgga 
      atcgactca aaagacctt tcctacaaa gacaagcac 
      tggcacgag gcctgcttc ctgtgcacg acttgcggg 
      gaatccctc gtcgatagg caattcggg tcaaagggc 
      gacaggatc tactgtggc cgctgctac gatgagcag 
      ttcgcgtcc aggtgcgac ggctgccat gaaatcttc 
      cgagcagga actctgagt gatcagttc aagtcgcgt 
      ctgaacctc cagacgaaa actgtcggt gaggagcgg 
      attaaaaag gccaaagag cggaatgaa gacgtcgcg 
      attttgtcc atggaactg ccgggcgaa aaaaaggcc 
      ttcaagtgc tgcctggac caggactgc ctgctgggg 
      gaccccaca agcctgcct ctaggaaca aagaagatg 
      gaatacaag acgaggcaa tggcacgag aagtgcttc 
      tgctgttgc gtatgcaaa gtaccgatc ggtacccag 
      agtttcatt cctcgcgag caagagatc tactgtgcc 
      aagtgttac gaagagaaa ttcgccacg cgttgcatc 
      aaatgcaac aaggtcatc accagcggc ggcgtgacg 
      tacaagaac gagccctgg cacagggag tgcttcacc 
      tgcacccac tgctccaag agcctggcg ggcgagcgc 
      ttcaccagc cgcgacgag aagccctat tgcgccgag 
      tgcttcggc gagctgttc gccaaacgg tgcttcgcc 
      tgcaataag cccatcacc ggcatcggg ggcacgaag 
      ttcatctcg ttcgaggac aggcattgg cacaacgac 
      tgcttcttc tgcgcctcg tgccgcacc agtctagtg 
      ggccgtggc ttcatcacc gaccaggaa gacatcatc 
      tgtcccgaa tgcgccaag caaaagttg atgtag
    mRNA sequence
    • >TC013758 RA
      acagaattc agtcgcagt tcactgtta gaaccgggt 
      gtgtgtatt gtcctcgaa aatgggaga cgttgaagt 
      tcattccaa ttttaccat caccgaaaa gaaaaccaa 
      aaaagtcaa gaagaccac caaacgaag ggagagtag 
      tgaccatgg gggcgaagt caccatcac tgaaatgga 
      gcgcaccaa cggcacgca gggtggcga gtacacaaa 
      ggccatgaa caaggactg gcatgggca acacttttg 
      ctgttggca gtgcgacga gagtttaac tggtcaacg 
      ctacgtcct ccgtgacga gcatcccta ttgcgtgtc 
      ttgttacga atctgtctt cgccaatgc ttgcgaaaa 
      gtgcagcag aatcatcgg aatcgactc aaaagacct 
      ttcctacaa agacaagca ctggcacga ggcctgctt 
      cctgtgcac gacttgcgg ggaatccct cgtcgatag 
      gcaattcgg gtcaaaggg cgacaggat ctactgtgg 
      ccgctgcta cgatgagca gttcgcgtc caggtgcga 
      cggctgcca tgaaatctt ccgagcagg aactctgag 
      tgatcagtt caagtcgcg tctgaacct ccagacgaa 
      aactgtcgg tgaggagcg gattaaaaa ggccaaaga 
      gcggaatga agacgtcgc gattttgtc catggaact 
      gccgggcga aaaaaaggc cttcaagtg ctgcctgga 
      ccaggactg cctgctggg ggaccccac aagcctgcc 
      tctaggaac aaagaagat ggaatacaa gacgaggca 
      atggcacga gaagtgctt ctgctgttg cgtatgcaa 
      agtaccgat cggtaccca gagtttcat tcctcgcga 
      gcaagagat ctactgtgc caagtgtta cgaagagaa 
      attcgccac gcgttgcat caaatgcaa caaggtcat 
      caccagcgg cggcgtgac gtacaagaa cgagccctg 
      gcacaggga gtgcttcac ctgcaccca ctgctccaa 
      gagcctggc gggcgagcg cttcaccag ccgcgacga 
      gaagcccta ttgcgccga gtgcttcgg cgagctgtt 
      cgccaaacg gtgcttcgc ctgcaataa gcccatcac 
      cggcatcgg gggcacgaa gttcatctc gttcgagga 
      caggcattg gcacaacga ctgcttctt ctgcgcctc 
      gtgccgcac cagtctagt gggccgtgg cttcatcac 
      cgaccagga agacatcat ctgtcccga atgcgccaa 
      gcaaaagtt gatgtagat tttacagat ttctgtttt 
      tttttaatc agtgaccat agcaccgtg acacgcaca 
      cactacaca cacacacat cttacacca ccgaaaaaa 
      acataaaag aaaaataca aaaaaaaac gaggatacg 
      ctgttttta gagtaaatg tatttattt ttggaggat 
      ttatttttg tttgccata ttataacat gacgtcaga 
      gttgattgg gacgtagga tattctaaa ttttgacat 
      tacaccaca gcttccttg attcgtgca atgacagta 
      cgtcgagga tcaaggaag ctgtgccct atccaaaca 
      gttgcctta aaaatttag tagtagaga cccatgaat 
      attaaacat atatatttg ttataaatt acggttgtt 
      attgcttct taactgtta atgtaagtg ttttgacaa 
      ttttttatt gtatattat tattactat tgtagataa 
      tgtacacag cttcatagc atttatgaa atgagcaac 
      tttggtgct atttgagta gttagatta attttttta 
      ttattattt tcctaattt ctagttact gtttttcag 
      taaattcta tctaaatgc ttcattaac agaaatgtt 
      actattaat attgataaa taaacatac ataatattc 
      gtttagagt ttaactttc agttgtaaa atgtacgta 
      ttggtaaat ttggattgt taaaaattt gttatgctt 
      ttaaataca agtacaggg ctttgcttt agggatatt 
      gttatattt aattccaca ataaatatc tgaaagaaa 
      a