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Gene information
General information
TC013754 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4804709..4807746
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013754
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02198

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    legs podomer partially shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013754 RA
      MIVPLICVA FLGASVSAN GELTILHHP PSLLFKGHD 
      HVKESILKE VYSSALGFS TEQYSNWDG LYIEDPFNL 
      AKAVVTVSV DGTSDIGNG KGHNFPLKT NVDEFDVFS 
      ALERRVLQR YPETEGHLV RISAGDSLH QLHKHKVFR 
      NLKLDKSKK VLNYLKASV EEDQAFLNE ITVLNSIAD 
      EIQNSGLHL DGTPDVFWF KIESLHPLI DLYGENSTK 
      VKEAKQLLN DAILHLNSV FTKVYKDKV LVSVITSDA 
      VHTRRARNI LQEKKPEPK DTQETKVNP AKFYSKDYP 
      VIFNIILWF GVAMTFTLL AICIAIAQM DPGRDSIIY 
      RMTSTRLKK DN
    CDS sequence
    • >TC013754 RA
      atgatcgtt ccgttaatt tgtgtcgca tttctgggc 
      gcctcagtt tccgcaaat ggtgaactg acgattctt 
      caccaccct ccaagtctg ctcttcaag ggacacgac 
      cacgtcaaa gagagcatt ttgaaggag gtgtattcg 
      agtgctttg ggtttctcg actgagcag tactcgaac 
      tgggatggt ttgtacatt gaggacccc tttaatttg 
      gccaaggct gtggtgacc gtcagcgtt gacggcact 
      tcggacatt ggcaacggc aaagggcac aatttcccg 
      cttaaaacg aacgttgac gaatttgat gttttctca 
      gctttggaa aggcgggtt ttgcaaagg tatcccgaa 
      actgagggt catttggtg cgaattagc gctggtgat 
      agtttgcat cagctccac aagcataaa gttttcaga 
      aatcttaaa ctcgacaag tcgaagaaa gtcctaaac 
      tatctcaaa gctagcgtg gaggaagac caagcgttt 
      ttgaacgaa attacagtc ttgaacagc attgcagac 
      gagattcaa aactcaggc ttacacttg gatggcact 
      cccgatgtc ttttggttc aaaatcgaa agtctgcac 
      cctttgatc gacttgtac ggcgagaat tcgaccaaa 
      gttaaagaa gccaaacag ctattaaat gatgcaatc 
      cttcacttg aattctgtt ttcaccaaa gtctacaag 
      gacaaggtt ttggtcagt gtcataaca agcgatgct 
      gtgcacacc agacgtgct agaaacatt ttgcaagaa 
      aagaaacct gaacccaag gacacacaa gagaccaaa 
      gtgaaccct gccaagttt tacagcaag gattatcca 
      gttattttt aatattatt ctgtggttt ggggtcgca 
      atgacgttt actctactt gctatttgc attgcaatt 
      gctcaaatg gatcctgga cgggactcc attatttac 
      aggatgacg agcactcgt ctcaagaag gataattaa
    mRNA sequence
    • >TC013754 RA
      atatgatta tgacatttg ggtggtgtg gttagttta 
      atgtcatat gacttcaaa tttccaaaa ctctgaaaa 
      ttagaccga aaaatgatc gttccgtta atttgtgtc 
      gcatttctg ggcgcctca gtttccgca aatggtgaa 
      ctgacgatt cttcaccac cctccaagt ctgctcttc 
      aagggacac gaccacgtc aaagagagc attttgaag 
      gaggtgtat tcgagtgct ttgggtttc tcgactgag 
      cagtactcg aactgggat ggtttgtac attgaggac 
      ccctttaat ttggccaag gctgtggtg accgtcagc 
      gttgacggc acttcggac attggcaac ggcaaaggg 
      cacaatttc ccgcttaaa acgaacgtt gacgaattt 
      gatgttttc tcagctttg gaaaggcgg gttttgcaa 
      aggtatccc gaaactgag ggtcatttg gtgcgaatt 
      agcgctggt gatagtttg catcagctc cacaagcat 
      aaagttttc agaaatctt aaactcgac aagtcgaag 
      aaagtccta aactatctc aaagctagc gtggaggaa 
      gaccaagcg tttttgaac gaaattaca gtcttgaac 
      agcattgca gacgagatt caaaactca ggcttacac 
      ttggatggc actcccgat gtcttttgg ttcaaaatc 
      gaaagtctg caccctttg atcgacttg tacggcgag 
      aattcgacc aaagttaaa gaagccaaa cagctatta 
      aatgatgca atccttcac ttgaattct gttttcacc 
      aaagtctac aaggacaag gttttggtc agtgtcata 
      acaagcgat gctgtgcac accagacgt gctagaaac 
      attttgcaa gaaaagaaa cctgaaccc aaggacaca 
      caagagacc aaagtgaac cctgccaag ttttacagc 
      aaggattat ccagttatt tttaatatt attctgtgg 
      tttggggtc gcaatgacg tttactcta cttgctatt 
      tgcattgca attgctcaa atggatcct ggacgggac 
      tccattatt tacaggatg acgagcact cgtctcaag 
      aaggataat taagtggga acgttgagt tcggatagt 
      attattgtg tgtatattc ctgttaaat tttctgtaa 
      aataccatt ctagatgtg taaattagt gtaacttat 
      gtaatactg tgatcacag cttccttga ttaaagaag 
      ggactttcc cttcacacg gctggtcgc attgaacaa 
      aaaaagtgt ttctaacag cttacttta ttgtagcgc 
      catctctta gctccttct gaaactttc cgtcttacc 
      atggttacc aaataaata tttaaattc aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY