Status
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Gene information
General information
TC013747 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4859435..4864546
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013747
    iBeetle screen iB_02197

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 1

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature potentially degenerated - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013747 RA
      MAEYQPLFG VWDYVVLVI VLLISSSIG VYYRFTGGK 
      QKTMQEYLL ADKNMSITP VAFSLMASF MSAITLLGV 
      SSENYTFGI QFIVINISY GIFTPVAAY LYLPVFFKL 
      QATSAYEYL EKRFGKASR LAASLSYSL QMTLYMGVV 
      LYAPALALE ALTGISKVA AILSVGLVC TFYSTIGGM 
      KAVLMTDVF QSILMFAAV FSIIIFAAI DKGSLAEIW 
      RIAERGNRT ELLNFNPDP TERHSWFSL IIGGGITFL 
      SLYAVNQTQ VQRYLTVKD LKTAQKALW LNWPILTCL 
      SLSTSFAGL AMYSKYYSC DPVSAKIIN SADQLMPLY 
      VMDTMGNIP GLAGLFVAG IFSASLSTV SAALNSLAA 
      VTVEDYYKP LYQYIAKKP LSEKRVSVQ TKLIALFFG 
      FACLAIAFL AQFLGGVLQ ASLTIFGVI GGPLLALFS 
      LGMFTTTAN EKGALVGLV SGISLALWM AFGNPKPPL 
      PTLPVTTEG CADVFSNIS ITNEPLVNN PDNKEYFWL 
      YKVSYLYNG VFGLVTTFL VGYLISLVI NSIYGRKDQ 
      HLDPNLFIP PIQRKLQKR ELSLSMKQW SESEEDLRS 
      KHVLKRKY
    CDS sequence
    • >TC013747 RA
      atggccgag tatcaacca cttttcggt gtttgggac 
      tatgttgtc ctagtgatt gttttatta atttcctcc 
      agtatcgga gtttattat agatttacc ggaggtaaa 
      cagaaaaca atgcaggag tatttattg gccgataaa 
      aacatgtct ataacaccg gtcgctttc tccctcatg 
      gcttctttc atgtcagca atcacacta ttaggtgtc 
      tccagcgaa aattacact ttcggtatc caattcatc 
      gtaatcaac atttcttac ggtattttc acgccagtg 
      gcggcgtat ttatacctc cctgttttc ttcaaacta 
      caagcaacg agcgcatat gagtatttg gaaaaaaga 
      ttcggcaaa gcctcacgc ctggcagcc tcgttgtct 
      tacagcctc cagatgact ttatacatg ggggtcgtg 
      ctctacgcc cctgcttta gcccttgaa gctttaaca 
      ggaatcagt aaagttgca gcgatttta tcggttgga 
      cttgtttgt acgttttat tcgacgatt ggaggcatg 
      aaagcggtc ttgatgacg gacgttttc cagtcgatt 
      ttaatgttt gctgcggtg tttagtatt attattttt 
      gcggctatt gataaggga agtttggcg gagatttgg 
      aggatcgcc gaacgcgga aatcggacc gaattattg 
      aacttcaat ccagatccc accgaaagg cattcgtgg 
      ttttcgctg ataatcggc gggggaatc acgtttttg 
      tcgctttat gccgttaat caaacgcaa gtgcaacga 
      tatttgact gtgaaggat ttgaaaacg gcacagaag 
      gcgctgtgg ctcaattgg cccattttg acgtgtttg 
      agcctcagt acgtccttt gcagggctc gctatgtac 
      tcgaaatat tacagttgt gacccagtt tcggccaaa 
      atcatcaac agtgctgat cagttaatg cctttgtac 
      gtgatggac acaatgggc aacatccct ggactggct 
      ggcctcttt gttgctggt attttcagc gcctctctc 
      tccaccgtt tcagcagca cttaatagt ttagctgct 
      gtaacagtc gaagattac tacaagcct ttgtaccaa 
      tacattgct aaaaaacct ttatcggaa aaaagagtc 
      tccgttcaa acgaaacta atcgcttta tttttcggc 
      ttcgcatgt ttggctatt gccttctta gcccagttt 
      ctaggagga gtccttcaa gcctctttg acgatattc 
      ggagttatc ggagggccc cttctagcc ctgttctcc 
      ctagggatg tttactacc actgccaat gaaaaggga 
      gctttggtc ggtcttgtg tcgggtatt tctctggct 
      ttgtggatg gcctttggt aaccccaaa ccgcctttg 
      ccaacgtta cctgtgacc acggaagga tgtgctgac 
      gttttctcg aatattagt attacaaat gagccgtta 
      gtgaacaat ccggacaat aaggaatat ttttggctg 
      tacaaagtg tcgtattta tacaacggt gttttcgga 
      ttagttacc acgttcctt gttggttat ttgatcagt 
      ttggtcatt aatagcatt tatgggaga aaagatcaa 
      cacttggac ccgaattta tttattcct ccaattcaa 
      cgaaagctg caaaagcgg gaattgtcg ctcagtatg 
      aagcagtgg tccgaatct gaagaggat ctcagaagc 
      aaacacgtg ctgaaacgg aaatattaa
    mRNA sequence
    • >TC013747 RA
      attcaacaa caaatgatt aattcttta cagaaaacc 
      tttgaccta cggaatccc tcataattt attcccgta 
      accagaaag cgttgattc tattgttta taacaatgc 
      atcatttat gagaggaaa aggtcacac aatttggac 
      gctttatta acattcatt gatcacaca ttaagtctt 
      gacgttggt ataaattgt agttttaaa acattgaca 
      atgcactgc tagaaaagt aaaagtgag tatgctaat 
      aattttttg cttatcttt actgattgc gacatttta 
      ttagatttt attatttaa tccagtatg cttttgtat 
      taaaatttt actgctcgc ttaaaaatg cttagcttt 
      attttttaa tccagttaa tacccgcga tgtgtggtg 
      tcatcactt gatttttta ggtatttgg tgctaaagt 
      ctcagattt tgttcataa ataaaaaat aagtttggc 
      gtaaacaat ggccgagta tcaaccact tttcggtgt 
      ttgggacta tgttgtcct agtgattgt tttattaat 
      ttcctccag tatcggagt ttattatag atttaccgg 
      aggtaaaca gaaaacaat gcaggagta tttattggc 
      cgataaaaa catgtctat aacaccggt cgctttctc 
      cctcatggc ttctttcat gtcagcaat cacactatt 
      aggtgtctc cagcgaaaa ttacacttt cggtatcca 
      attcatcgt aatcaacat ttcttacgg tattttcac 
      gccagtggc ggcgtattt atacctccc tgttttctt 
      caaactaca agcaacgag cgcatatga gtatttgga 
      aaaaagatt cggcaaagc ctcacgcct ggcagcctc 
      gttgtctta cagcctcca gatgacttt atacatggg 
      ggtcgtgct ctacgcccc tgctttagc ccttgaagc 
      tttaacagg aatcagtaa agttgcagc gattttatc 
      ggttggact tgtttgtac gttttattc gacgattgg 
      aggcatgaa agcggtctt gatgacgga cgttttcca 
      gtcgatttt aatgtttgc tgcggtgtt tagtattat 
      tatttttgc ggctattga taagggaag tttggcgga 
      gatttggag gatcgccga acgcggaaa tcggaccga 
      attattgaa cttcaatcc agatcccac cgaaaggca 
      ttcgtggtt ttcgctgat aatcggcgg gggaatcac 
      gtttttgtc gctttatgc cgttaatca aacgcaagt 
      gcaacgata tttgactgt gaaggattt gaaaacggc 
      acagaaggc gctgtggct caattggcc cattttgac 
      gtgtttgag cctcagtac gtcctttgc agggctcgc 
      tatgtactc gaaatatta cagttgtga cccagtttc 
      ggccaaaat catcaacag tgctgatca gttaatgcc 
      tttgtacgt gatggacac aatgggcaa catccctgg 
      actggctgg cctctttgt tgctggtat tttcagcgc 
      ctctctctc caccgtttc agcagcact taatagttt 
      agctgctgt aacagtcga agattacta caagccttt 
      gtaccaata cattgctaa aaaaccttt atcggaaaa 
      aagagtctc cgttcaaac gaaactaat cgctttatt 
      tttcggctt cgcatgttt ggctattgc cttcttagc 
      ccagtttct aggaggagt ccttcaagc ctctttgac 
      gatattcgg agttatcgg agggcccct tctagccct 
      gttctccct agggatgtt tactaccac tgccaatga 
      aaagggagc tttggtcgg tcttgtgtc gggtatttc 
      tctggcttt gtggatggc ctttggtaa ccccaaacc 
      gcctttgcc aacgttacc tgtgaccac ggaaggatg 
      tgctgacgt tttctcgaa tattagtat tacaaatga 
      gccgttagt gaacaatcc ggacaataa ggaatattt 
      ttggctgta caaagtgtc gtatttata caacggtgt 
      tttcggatt agttaccac gttccttgt tggttattt 
      gatcagttt ggtcattaa tagcattta tgggagaaa 
      agatcaaca cttggaccc gaatttatt tattcctcc 
      aattcaacg aaagctgca aaagcggga attgtcgct 
      cagtatgaa gcagtggtc cgaatctga agaggatct 
      cagaagcaa acacgtgct gaaacggaa atattaatt 
      gtattaatt ttgtagcag tacttattt attgtgtac 
      acgtgaaaa tgtcttcca ttagataat aaatgccgg 
      ttttattga aataaacaa ctttgttag at
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