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Gene information
General information
TC013738 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 3965001..3966378
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013738
    iBeetle screen iB_02195
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber sometimes degenerated - Number of animals showing the phenotype: 4

    tropharium sometimes irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013738 RA
      MNKTNKFLL EAAAIFVTY FYFGILQEK ITKGKYTYE 
      ATDDKGEKI YLTEKYTYF LTLVFILCA TSYAAACIF 
      LKIWRPDED KTPRLYYIS IAITYLLAM VCSNMALQW 
      VPYPTQVVG KSAKPIPVM ILGVLLGKK SYPFKKYIF 
      VLLIVVGVV LFMFKDKGK PAQQDMEFG LGELLLILS 
      LMMDGMTGG VQERIRAEA QPTGQQMMK ASNGWSVLF 
      LGCALVTTG EGFQFLEFA KRHPNVTVN LLVLGLTQA 
      VGQMFLYNM VSDFGPLVV SVVTTTRKF FTFLGSVII 
      FGNALSVRQ WIGTLIVFT GLFLDTFFS KKATKKSPS 
      N
    CDS sequence
    • >TC013738 RA
      atgaataaa actaacaaa ttcctcctg gaagctgct 
      gcaattttc gtaacatat ttctacttt ggaatatta 
      caagaaaaa ataacgaaa ggcaaatac acgtatgag 
      gctacggac gataaagga gagaaaatc taccttacg 
      gagaagtac acttacttc ctaacctta gtttttatt 
      ttgtgcgca actagctat gcagccgct tgcatattt 
      ttgaaaatt tggcggccc gatgaagac aagacaccg 
      agactttac tacataagt attgccata acgtacctt 
      ctggctatg gtatgttca aatatggca ctccaatgg 
      gtgccatat cccacccaa gttgtgggc aaatcggcc 
      aaacccatc ccagtcatg atactggga gtattgtta 
      gggaaaaaa tcatatccg tttaagaag tacattttc 
      gtgttgttg attgtcgtt ggagtggtg ctgtttatg 
      tttaaagac aagggcaaa cctgcgcag caagacatg 
      gaatttggt ttgggggaa ctgttgtta atcttgtcc 
      ctaatgatg gatgggatg actggagga gttcaagaa 
      aggataaga gcagaggcg cagccaaca ggccaacaa 
      atgatgaaa gcatcaaat ggatggtcg gttttattt 
      ctaggatgc gctcttgta acaacaggt gaaggtttc 
      cagtttttg gaatttgca aagaggcat ccaaatgtt 
      actgttaat ctattagta ttaggatta acgcaagca 
      gtcggacaa atgtttctg tataatatg gtttcagat 
      tttgggcct ttggttgtc tcagttgtg acaacaacc 
      cgaaagttc tttactttc ttgggctca gttattatt 
      tttgggaac gccctaagt gttagacag tggatagga 
      acattgatt gttttcact ggactcttc cttgatacg 
      ttcttctct aagaaagcc accaaaaaa tctcccagc 
      aattaa
    mRNA sequence
    • >TC013738 RA
      atggctgcc aacttaaaa tagtaattg tcaattgaa 
      cacgtttac atcaattca aaacgatgt tttatcgtc 
      aaaaatgaa tgatgtgca gtgtaacag tcagtgatt 
      gagtctctc ttaaaaccg cttcattcc attaaccaa 
      taattggca caaatcaat atgaataaa actaacaaa 
      ttcctcctg gaagctgct gcaattttc gtaacatat 
      ttctacttt ggaatatta caagaaaaa ataacgaaa 
      ggcaaatac acgtatgag gctacggac gataaagga 
      gagaaaatc taccttacg gagaagtac acttacttc 
      ctaacctta gtttttatt ttgtgcgca actagctat 
      gcagccgct tgcatattt ttgaaaatt tggcggccc 
      gatgaagac aagacaccg agactttac tacataagt 
      attgccata acgtacctt ctggctatg gtatgttca 
      aatatggca ctccaatgg gtgccatat cccacccaa 
      gttgtgggc aaatcggcc aaacccatc ccagtcatg 
      atactggga gtattgtta gggaaaaaa tcatatccg 
      tttaagaag tacattttc gtgttgttg attgtcgtt 
      ggagtggtg ctgtttatg tttaaagac aagggcaaa 
      cctgcgcag caagacatg gaatttggt ttgggggaa 
      ctgttgtta atcttgtcc ctaatgatg gatgggatg 
      actggagga gttcaagaa aggataaga gcagaggcg 
      cagccaaca ggccaacaa atgatgaaa gcatcaaat 
      ggatggtcg gttttattt ctaggatgc gctcttgta 
      acaacaggt gaaggtttc cagtttttg gaatttgca 
      aagaggcat ccaaatgtt actgttaat ctattagta 
      ttaggatta acgcaagca gtcggacaa atgtttctg 
      tataatatg gtttcagat tttgggcct ttggttgtc 
      tcagttgtg acaacaacc cgaaagttc tttactttc 
      ttgggctca gttattatt tttgggaac gccctaagt 
      gttagacag tggatagga acattgatt gttttcact 
      ggactcttc cttgatacg ttcttctct aagaaagcc 
      accaaaaaa tctcccagc aattaatct atttgttgt 
      taattctac ataaatttt attatttta tttatgata 
      aaaacttat cagtgattg ctgtttgat tgtatgaac 
      ctgcattga ttgattat