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Gene information
General information
TC013707 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5520285..5525910
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013707
    iBeetle screen iB_02189

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013707 RA
      MHAVLVSVY LLLVTPITL TWWAAGSQL VMDPRTICK 
      KTKRLRGKM AEICSKQAL VDQIRLGVE LGQRECQYQ 
      FRFRRWNCT SSRKSIRKV LLRDTRETG FVNAVLAAG 
      VTYQVTRAC TTGELLGCS CDRKMKSKK NKKRLKMAS 
      MPEGDWEWE ACGGENIDF GLKKSKDFL DTRYKKRSD 
      MKTLVKLHN YVAGRMAIK NHMRTECKC HGLSGSCTL 
      KTCWRKMPP FREVGNRLK ERFDGAVKV IAGNDGQSF 
      MPEDSSIKP PGKTGLVYS EESPHFCLP NNTLGSFGT 
      QGRTCVETS PGEEGCSIL CCGRGSRSH DETEEKNCK 
      CKFLWCCEV KCEKCNETR TISTCL
    CDS sequence
    • >TC013707 RA
      atgcacgcc gtgctggtg tccgtttac cttctgctt 
      gtcaccccc atcacatta acttggtgg gccgccggg 
      agccaattg gtcatggat ccgcgcaca atttgcaaa 
      aagaccaaa cggcttagg ggtaaaatg gccgagatt 
      tgcagcaag caggcgtta gtggaccaa atacggctc 
      ggggtcgag ctcggacaa agggagtgc cagtatcag 
      tttaggttc aggaggtgg aattgcaca tcttcgaga 
      aaaagcatc agaaaagtg cttttgcgc gataccaga 
      gagacgggc tttgtcaat gccgtgtta gcagccggt 
      gtgacctac caggtaaca agggcatgc accacgggg 
      gaactcctt ggatgctcc tgcgacaga aaaatgaag 
      agcaaaaaa aacaagaag agactgaaa atggcatcg 
      atgccggag ggtgactgg gagtgggag gcctgcggg 
      ggagaaaac atcgacttt gggctgaaa aagtccaaa 
      gactttctc gacacgcga tacaaaaag cgaagtgat 
      atgaaaact ttagtcaaa ctacacaac tacgtcgcc 
      ggccgaatg gcaatcaaa aaccacatg cggacagaa 
      tgcaagtgc cacggtctt tcggggtca tgcaccctg 
      aagacatgc tggcgcaaa atgcccccc ttcagggag 
      gtgggcaat cggctgaag gagcggttc gacggcgct 
      gttaaagtg attgcaggc aatgacggc caaagcttc 
      atgcctgag gactcctcc atcaagccg ccaggcaag 
      acgggcttg gtgtactcg gaggaatcg ccgcacttt 
      tgtttgcca aacaacact ctgggctcg tttggcaca 
      cagggacgc acctgtgtt gaaacttcg ccaggtgag 
      gaaggctgc agcatactt tgctgcggc aggggctcc 
      agaagtcat gacgaaacc gaagaaaaa aattgcaag 
      tgcaagttt ttgtggtgc tgtgaagtt aaatgtgag 
      aaatgcaac gagacgcgt acgattagt acttgtttg 
      tga
    mRNA sequence
    • >TC013707 RA
      gggctcgct taggtgagt gagcgcgtg gtcatgcac 
      gccgtgctg gtgtccgtt taccttctg cttgtcacc 
      cccatcaca ttaacttgg tgggccgcc gggagccaa 
      ttggtcatg gatccgcgc acaatttgc aaaaagacc 
      aaacggctt aggggtaaa atggccgag atttgcagc 
      aagcaggcg ttagtggac caaatacgg ctcggggtc 
      gagctcgga caaagggag tgccagtat cagtttagg 
      ttcaggagg tggaattgc acatcttcg agaaaaagc 
      atcagaaaa gtgcttttg cgcgatacc agagagacg 
      ggctttgtc aatgccgtg ttagcagcc ggtgtgacc 
      taccaggta acaagggca tgcaccacg ggggaactc 
      cttggatgc tcctgcgac agaaaaatg aagagcaaa 
      aaaaacaag aagagactg aaaatggca tcgatgccg 
      gagggtgac tgggagtgg gaggcctgc gggggagaa 
      aacatcgac tttgggctg aaaaagtcc aaagacttt 
      ctcgacacg cgatacaaa aagcgaagt gatatgaaa 
      actttagtc aaactacac aactacgtc gccggccga 
      atggcaatc aaaaaccac atgcggaca gaatgcaag 
      tgccacggt ctttcgggg tcatgcacc ctgaagaca 
      tgctggcgc aaaatgccc cccttcagg gaggtgggc 
      aatcggctg aaggagcgg ttcgacggc gctgttaaa 
      gtgattgca ggcaatgac ggccaaagc ttcatgcct 
      gaggactcc tccatcaag ccgccaggc aagacgggc 
      ttggtgtac tcggaggaa tcgccgcac ttttgtttg 
      ccaaacaac actctgggc tcgtttggc acacaggga 
      cgcacctgt gttgaaact tcgccaggt gaggaaggc 
      tgcagcata ctttgctgc ggcaggggc tccagaagt 
      catgacgaa accgaagaa aaaaattgc aagtgcaag 
      tttttgtgg tgctgtgaa gttaaatgt gagaaatgc 
      aacgagacg cgtacgatt agtacttgt ttgtgattc 
      tatttcata gtgttttgt gttttattg actattatt 
      aatttttat ctatctgta catatcgat atatttgtt 
      actaattgt tgtacacgg ccgcagaat ataacgcca 
      cgtcttaac tagcaaaat tgtaaacgt gtgtttact 
      aataaaatt attgaacat tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY