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Gene information
General information
TC013701 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 4688037..4696595
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013701
    iBeetle screen iB_02188
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head sometimes partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013701 RA
      MREMVGGCC VCSDDRGWS ENPLVYCDG QSCTVAVHQ 
      ACYGIVTVP TGPWYCRKC ESQERSVKV KCELCPSKH 
      GALKRTDNS GWAHVVCAL YIPEVRFGN VTTMEPIQL 
      QLIPTERFN KTCYICEEK GKASSATVG ACMQCNKAG 
      CKQQFHVTC AQSLGLLCE EAGNYLDNV KYCGYCQHH 
      YSKLKKGGN VKTIPPYKP VSTENHLSD SNSEKEAEN 
      NASTKSSTS SSQLSSSKR KTSSSSKAS SSKTSSSTK 
      SSSSSSSSH QKTVHNSSK SSDKLKVSS SSVRSSAKS 
      AEDNSASVE SGNADSKTE SRDNKVDLD QSKTDVKDS 
      KSSKKRKNN SRTPTPVAL VSSETVSVV NTSSVVAEN 
      LGGTASAGE RKGVVGEPA EKPKKIKVE TTQSILTNQ 
      PIVALTSIS TIKTISNSS ITSSLNDNV ETAPSPSLP 
      PQSIIQSGP PHSVNSLVV SVPLTSTSL SPHPQIINN 
      QASVVTPTP IIHSERSVN SPADRDTSN TTPVNMTGG 
      SSNQGSSII TSEMSGGGL KITYEKQDA VTMEVDMEL 
      EVSPSNQDL ASKRSHSVE KSEKGGRGK KRNSGGANG 
      IINQLNNTS ISSSTVKRS SRSQNSTPP QPGAFASIS 
      QSQIKDSPP SSPSSESQS AASVQNGRG KSKSRKSAP 
      SSLARSQSP KDMKDIKFF QNGVTAPHM LGNQLNPNS 
      NMAQKMSDH LNSELEAHS IFSASDNST MMGPQLHHR 
      VIASARASS AGGSSTASA GSGFSSMLG GGGGNIPQT 
      LDQLLERQW EQGSQFLME QAQHFDIAS LLSCLHQLR 
      AENLRLEEH VSSLLQRRD HLLAVNARL AIPLTGQPA 
      TPQSSHAHT VNNIHPAVG ASHGDVPRS GRHSGNGAN 
      YPAHPQGSQ HPSVAPVEN GLASDPSQP YPSSHSHRS 
      PATSQSQPS PSVRHSPAG SGYPNSGLL RQNADTSGR 
      SAPRPQPYP LYQGQGPSS QQQMVIRRD SDIYHQSTS 
      KPS
    • >TC013701 RB
      MREMVGGCC VCSDDRGWS ENPLVYCDG QSCTVAVHQ 
      ACYGIVTVP TGPWYCRKC ESQERSVKV KCELCPSKH 
      GALKRTDNS GWAHVVCAL YIPEVRFGN VTTMEPIQL 
      QLIPTERFN KTCYICEEK GKASSATVG ACMQCNKAG 
      CKQQFHVTC AQSLGLLCE EAGNYLDNV KYCGYCQHH 
      YSKLKKGGN VKTIPPYKP VSTENHLSD SNSEKEAEN 
      NASTKSSTS SSQLSSSKR KTSSSSKAS SSKTSSSTK 
      SSSSSSSSH QKTVHNSSK SSDKLKVSS SSVRSSAKS 
      AEDNSASVE SGNADSKTE SRDNKVDLD QSKTDVKDS 
      KSSKKRKNN SRTPTPVAL VSSETVSVV NTSSVVAEN 
      LGGTASAGE RKGVVGEPA EKPKKIKVE TTQSILTNQ 
      PIVALTSIS TIKTISNSS ITSSLNDNV ETAPSPSLP 
      PQSIIQSGP PHSVNSLVV SVPLTSTSL SPHPQIINN 
      QASVVTPTP IIHSERSVN SPADRDTSN TTPVNMTGG 
      SSNQGSSII TSEMSGGGL KITYEKQDA VTMEVDMEL 
      EVSPSNQDL ASKRSHSVE KSEKGGRGK KRNSGGANG 
      IINQLNNTS ISSSTVKRS SRSQNSTPP QPGAFASIS 
      QSQIKDSPP SSPSSESQS AASVQNGRG KSKSRKSAP 
      SSLARSQSP KDMKDIKFF QNGVTAPHM LGNQLNPNS 
      NMAQKMSDH LNSELEAHS IFSASDNST MMGPQLHHR 
      VIASARASS AGGSSTASA GSGFSSMLG GGGGNIPQT 
      LDQLLERQW EQGSQFLME QAQHFDIAS LLSCLHQLR 
      AENLRLEEH VSSLLQRRD HLLAVNARL AIPLTGQPA 
      TPQSSHAHT VNNIHPAVG ASHGDVPRS GRHSGNGAN 
      YPAHPQGSQ HPSVAPVEN GLASDPSQP YPSSHSHRS 
      PATSQSQPS PSVRHSPAG SGYPNSGLL RQNADTSGR 
      SAPRPQPYP LYQGQGPSS QQQVF
    CDS sequence
    • >TC013701 RA
      atgagggaa atggtagga ggttgttgc gtttgttct 
      gacgataga ggatggtct gaaaatcca cttgtgtac 
      tgcgatgga caatcttgc actgttgct gttcatcaa 
      gcctgctat ggtatagtt actgttccc actggtccg 
      tggtattgt cgcaaatgc gagagtcaa gaaagatct 
      gttaaagtg aaatgcgaa ttgtgtccc agcaaacat 
      ggcgctctc aaacgcacc gacaattca ggttgggct 
      cacgttgtc tgtgctctt tacattcca gaagtgcgt 
      tttggcaat gtcacaacg atggaaccg attcagctt 
      caattaatt ccaacggaa cgttttaat aagacgtgt 
      tacatatgt gaagaaaag ggaaaagct tcgagtgca 
      acggttggt gcatgtatg caatgtaat aaggccggt 
      tgcaagcag cagtttcat gtgacttgt gcacagagt 
      ttgggttta ctttgcgaa gaagctgga aactattta 
      gacaacgtc aaatattgt ggctattgc caacaccat 
      tacagtaaa ttgaagaaa ggtggtaac gtgaaaaca 
      atccctcct tataagccc gtatccacg gaaaatcac 
      ctctccgac tcgaattcg gagaaggaa gcggaaaac 
      aacgcttcg acgaaatcg tcgacttct tcatctcaa 
      ctgtcatca tccaaacgc aagacgagt tcgagcagc 
      aaagcctcc tcgtctaag acgagttcg agcacgaaa 
      agctcgtcg agttcgtcg tcttctcat cagaaaacc 
      gtccataat tcgagtaaa agttcagat aaactgaaa 
      gtttcgagt tctagtgtt aggagcagt gccaaaagt 
      gcggaagac aacagtgct agtgttgag agtggtaac 
      gcggacagc aaaacggag tcgagagac aacaaagtt 
      gacttagat caaagcaaa acggacgtt aaagattcg 
      aagagttcg aagaaacgt aagaataat tcgaggacg 
      ccaacgccg gttgcgtta gtgtccagt gaaactgtt 
      agtgttgtt aatacatcc agcgtcgtg gcggagaat 
      ttgggcggc acggcgtcg gcgggcgag cggaaaggg 
      gtggtcgga gagccggcg gagaagcca aaaaagatc 
      aaggtggag acaacgcag tcaatttta acaaaccag 
      ccaatcgtt gccttaact tcaatatca acgattaaa 
      accatatcc aactcttcg ataacgtcg tcattgaac 
      gacaacgtc gagacggcc ccgtcacca tcactacct 
      ccacaatcc ataattcag tcaggtcca cctcacagt 
      gtcaattct ctagtggtt tccgtgcct ctgactagt 
      actagctta agcccccat ccccaaatc atcaacaac 
      caagcgagt gtcgtaaca cccacacct atcatacat 
      tcagaaagg agtgtcaat agtccagcc gacagagac 
      acgtcaaat accacacca gtaaacatg actggtggt 
      agtagtaac caggggagt tcgataatt acctcagaa 
      atgagtgga ggtggccta aaaattacc tacgagaag 
      caggatgcg gtgaccatg gaagtggat atggagttg 
      gaagtttct ccgtcgaat caagacttg gcttcgaaa 
      agatctcat tcagtggaa aaatccgag aaaggtggt 
      cggggaaag aaacgaaat tcgggagga gcgaatggg 
      attattaat cagttgaat aatacgtcg atttcttcg 
      tcgacggtg aaacgttcg agtagatct cagaattcg 
      acaccgcca cagccgggg gcctttgcc agtataagt 
      cagtcgcag attaaggat tcacctccc agtagtccc 
      agctccgaa agtcagagt gctgcgtcg gtgcagaat 
      ggtcgaggg aagtcgaaa tcgaggaaa agtgcgcct 
      tcgtcgctt gcaaggtcg cagtcgccg aaagatatg 
      aaagatatt aaatttttt cagaacggg gttacagcc 
      ccgcacatg ttgggtaat cagttaaac cccaattcc 
      aacatggca caaaaaatg tccgatcat ctaaattcc 
      gaattagaa gcccacagt attttcagt gcaagtgat 
      aattcaaca atgatgggt ccccaactc catcatcga 
      gtaatcgct tcggcaagg gcgagcagt gcaggtggt 
      tcgagtact gcttcagca ggtagcggt ttttcatca 
      atgttagga ggtggaggt ggcaacata cctcaaaca 
      cttgaccaa ttacttgaa agacagtgg gaacaagga 
      tctcaattt ttaatggaa caagcccaa cattttgat 
      attgcatcc ttgctgtct tgtctccac caactacgt 
      gctgaaaat ttgaggctg gaagaacac gtcagttcg 
      ttacttcaa agaagagat catttattg gctgtcaat 
      gctaggttg gctattcca ttgactggc caacccgca 
      acccctcag tcttcccat gctcataca gtgaataac 
      atacaccct gctgtgggg gccagccac ggggacgtg 
      ccccgttca gggcgccac agcggcaac ggtgccaac 
      taccccgcg catccccaa ggttcacaa cacccaagt 
      gtagcccca gttgaaaat ggtttagct tcagatccc 
      tcccaaccc tatccctca tctcattcg catcgaagt 
      ccagcaaca agtcagtcc caacccagt ccatcggtt 
      agacattcg ccggctggt tcagggtat cccaatagt 
      ggtctgctt cggcaaaat gcagacact agcggacgc 
      tctgcgcct agacctcaa ccgtatccg ttatatcag 
      gggcagggg ccttcgtcg caacaacag atggtaatt 
      agaagagac tcagatata taccatcag tcaacatcg 
      aaacctagc tga
    • >TC013701 RB
      atgagggaa atggtagga ggttgttgc gtttgttct 
      gacgataga ggatggtct gaaaatcca cttgtgtac 
      tgcgatgga caatcttgc actgttgct gttcatcaa 
      gcctgctat ggtatagtt actgttccc actggtccg 
      tggtattgt cgcaaatgc gagagtcaa gaaagatct 
      gttaaagtg aaatgcgaa ttgtgtccc agcaaacat 
      ggcgctctc aaacgcacc gacaattca ggttgggct 
      cacgttgtc tgtgctctt tacattcca gaagtgcgt 
      tttggcaat gtcacaacg atggaaccg attcagctt 
      caattaatt ccaacggaa cgttttaat aagacgtgt 
      tacatatgt gaagaaaag ggaaaagct tcgagtgca 
      acggttggt gcatgtatg caatgtaat aaggccggt 
      tgcaagcag cagtttcat gtgacttgt gcacagagt 
      ttgggttta ctttgcgaa gaagctgga aactattta 
      gacaacgtc aaatattgt ggctattgc caacaccat 
      tacagtaaa ttgaagaaa ggtggtaac gtgaaaaca 
      atccctcct tataagccc gtatccacg gaaaatcac 
      ctctccgac tcgaattcg gagaaggaa gcggaaaac 
      aacgcttcg acgaaatcg tcgacttct tcatctcaa 
      ctgtcatca tccaaacgc aagacgagt tcgagcagc 
      aaagcctcc tcgtctaag acgagttcg agcacgaaa 
      agctcgtcg agttcgtcg tcttctcat cagaaaacc 
      gtccataat tcgagtaaa agttcagat aaactgaaa 
      gtttcgagt tctagtgtt aggagcagt gccaaaagt 
      gcggaagac aacagtgct agtgttgag agtggtaac 
      gcggacagc aaaacggag tcgagagac aacaaagtt 
      gacttagat caaagcaaa acggacgtt aaagattcg 
      aagagttcg aagaaacgt aagaataat tcgaggacg 
      ccaacgccg gttgcgtta gtgtccagt gaaactgtt 
      agtgttgtt aatacatcc agcgtcgtg gcggagaat 
      ttgggcggc acggcgtcg gcgggcgag cggaaaggg 
      gtggtcgga gagccggcg gagaagcca aaaaagatc 
      aaggtggag acaacgcag tcaatttta acaaaccag 
      ccaatcgtt gccttaact tcaatatca acgattaaa 
      accatatcc aactcttcg ataacgtcg tcattgaac 
      gacaacgtc gagacggcc ccgtcacca tcactacct 
      ccacaatcc ataattcag tcaggtcca cctcacagt 
      gtcaattct ctagtggtt tccgtgcct ctgactagt 
      actagctta agcccccat ccccaaatc atcaacaac 
      caagcgagt gtcgtaaca cccacacct atcatacat 
      tcagaaagg agtgtcaat agtccagcc gacagagac 
      acgtcaaat accacacca gtaaacatg actggtggt 
      agtagtaac caggggagt tcgataatt acctcagaa 
      atgagtgga ggtggccta aaaattacc tacgagaag 
      caggatgcg gtgaccatg gaagtggat atggagttg 
      gaagtttct ccgtcgaat caagacttg gcttcgaaa 
      agatctcat tcagtggaa aaatccgag aaaggtggt 
      cggggaaag aaacgaaat tcgggagga gcgaatggg 
      attattaat cagttgaat aatacgtcg atttcttcg 
      tcgacggtg aaacgttcg agtagatct cagaattcg 
      acaccgcca cagccgggg gcctttgcc agtataagt 
      cagtcgcag attaaggat tcacctccc agtagtccc 
      agctccgaa agtcagagt gctgcgtcg gtgcagaat 
      ggtcgaggg aagtcgaaa tcgaggaaa agtgcgcct 
      tcgtcgctt gcaaggtcg cagtcgccg aaagatatg 
      aaagatatt aaatttttt cagaacggg gttacagcc 
      ccgcacatg ttgggtaat cagttaaac cccaattcc 
      aacatggca caaaaaatg tccgatcat ctaaattcc 
      gaattagaa gcccacagt attttcagt gcaagtgat 
      aattcaaca atgatgggt ccccaactc catcatcga 
      gtaatcgct tcggcaagg gcgagcagt gcaggtggt 
      tcgagtact gcttcagca ggtagcggt ttttcatca 
      atgttagga ggtggaggt ggcaacata cctcaaaca 
      cttgaccaa ttacttgaa agacagtgg gaacaagga 
      tctcaattt ttaatggaa caagcccaa cattttgat 
      attgcatcc ttgctgtct tgtctccac caactacgt 
      gctgaaaat ttgaggctg gaagaacac gtcagttcg 
      ttacttcaa agaagagat catttattg gctgtcaat 
      gctaggttg gctattcca ttgactggc caacccgca 
      acccctcag tcttcccat gctcataca gtgaataac 
      atacaccct gctgtgggg gccagccac ggggacgtg 
      ccccgttca gggcgccac agcggcaac ggtgccaac 
      taccccgcg catccccaa ggttcacaa cacccaagt 
      gtagcccca gttgaaaat ggtttagct tcagatccc 
      tcccaaccc tatccctca tctcattcg catcgaagt 
      ccagcaaca agtcagtcc caacccagt ccatcggtt 
      agacattcg ccggctggt tcagggtat cccaatagt 
      ggtctgctt cggcaaaat gcagacact agcggacgc 
      tctgcgcct agacctcaa ccgtatccg ttatatcag 
      gggcagggg ccttcgtcg caacaacag gtattttga
    mRNA sequence
    • >TC013701 RA
      agtcaaacc aaaatataa caaaaaaca aaatggttg 
      ctctataaa agaatgtgt ttaaaatgc atttttatg 
      caaaagtac gtgcattgt ggctcattt acgtgattt 
      actaaattg atattaggt aaacaccgg taggttttt 
      gtaaatcaa gcagttccg atccaaaat cgcgcattt 
      ttctctgag aaattgaca tgacgctat gaacacgct 
      agtgtaaat aaataaagt gtgattcga gcaattcaa 
      accaggagg tttctattt atatgagaa ggttatata 
      ggttaacag tgcaaacaa agcaaacga atttattta 
      cgttaaatc tcattacat aacgtagag taattgtgt 
      atttgttgg catcacgta aacaaagcg agcgccttt 
      tccaaaaac ggaagtctt actaatgct attgctctg 
      gttttttag gctgcgagg atgaacaac aaaacggtc 
      tagaaatga atgtgaacg tattgcatt ggagatgtg 
      gggtgggat tgtcaaatc gctaattta gcaaacttc 
      gcttctttg cattatgag ggaaatggt aggaggttg 
      ttgcgtttg ttctgacga tagaggatg gtctgaaaa 
      tccacttgt gtactgcga tggacaatc ttgcactgt 
      tgctgttca tcaagcctg ctatggtat agttactgt 
      tcccactgg tccgtggta ttgtcgcaa atgcgagag 
      tcaagaaag atctgttaa agtgaaatg cgaattgtg 
      tcccagcaa acatggcgc tctcaaacg caccgacaa 
      ttcaggttg ggctcacgt tgtctgtgc tctttacat 
      tccagaagt gcgttttgg caatgtcac aacgatgga 
      accgattca gcttcaatt aattccaac ggaacgttt 
      taataagac gtgttacat atgtgaaga aaagggaaa 
      agcttcgag tgcaacggt tggtgcatg tatgcaatg 
      taataaggc cggttgcaa gcagcagtt tcatgtgac 
      ttgtgcaca gagtttggg tttactttg cgaagaagc 
      tggaaacta tttagacaa cgtcaaata ttgtggcta 
      ttgccaaca ccattacag taaattgaa gaaaggtgg 
      taacgtgaa aacaatccc tccttataa gcccgtatc 
      cacggaaaa tcacctctc cgactcgaa ttcggagaa 
      ggaagcgga aaacaacgc ttcgacgaa atcgtcgac 
      ttcttcatc tcaactgtc atcatccaa acgcaagac 
      gagttcgag cagcaaagc ctcctcgtc taagacgag 
      ttcgagcac gaaaagctc gtcgagttc gtcgtcttc 
      tcatcagaa aaccgtcca taattcgag taaaagttc 
      agataaact gaaagtttc gagttctag tgttaggag 
      cagtgccaa aagtgcgga agacaacag tgctagtgt 
      tgagagtgg taacgcgga cagcaaaac ggagtcgag 
      agacaacaa agttgactt agatcaaag caaaacgga 
      cgttaaaga ttcgaagag ttcgaagaa acgtaagaa 
      taattcgag gacgccaac gccggttgc gttagtgtc 
      cagtgaaac tgttagtgt tgttaatac atccagcgt 
      cgtggcgga gaatttggg cggcacggc gtcggcggg 
      cgagcggaa aggggtggt cggagagcc ggcggagaa 
      gccaaaaaa gatcaaggt ggagacaac gcagtcaat 
      tttaacaaa ccagccaat cgttgcctt aacttcaat 
      atcaacgat taaaaccat atccaactc ttcgataac 
      gtcgtcatt gaacgacaa cgtcgagac ggccccgtc 
      accatcact acctccaca atccataat tcagtcagg 
      tccacctca cagtgtcaa ttctctagt ggtttccgt 
      gcctctgac tagtactag cttaagccc ccatcccca 
      aatcatcaa caaccaagc gagtgtcgt aacacccac 
      acctatcat acattcaga aaggagtgt caatagtcc 
      agccgacag agacacgtc aaataccac accagtaaa 
      catgactgg tggtagtag taaccaggg gagttcgat 
      aattacctc agaaatgag tggaggtgg cctaaaaat 
      tacctacga gaagcagga tgcggtgac catggaagt 
      ggatatgga gttggaagt ttctccgtc gaatcaaga 
      cttggcttc gaaaagatc tcattcagt ggaaaaatc 
      cgagaaagg tggtcgggg aaagaaacg aaattcggg 
      aggagcgaa tgggattat taatcagtt gaataatac 
      gtcgatttc ttcgtcgac ggtgaaacg ttcgagtag 
      atctcagaa ttcgacacc gccacagcc gggggcctt 
      tgccagtat aagtcagtc gcagattaa ggattcacc 
      tcccagtag tcccagctc cgaaagtca gagtgctgc 
      gtcggtgca gaatggtcg agggaagtc gaaatcgag 
      gaaaagtgc gccttcgtc gcttgcaag gtcgcagtc 
      gccgaaaga tatgaaaga tattaaatt ttttcagaa 
      cggggttac agccccgca catgttggg taatcagtt 
      aaaccccaa ttccaacat ggcacaaaa aatgtccga 
      tcatctaaa ttccgaatt agaagccca cagtatttt 
      cagtgcaag tgataattc aacaatgat gggtcccca 
      actccatca tcgagtaat cgcttcggc aagggcgag 
      cagtgcagg tggttcgag tactgcttc agcaggtag 
      cggtttttc atcaatgtt aggaggtgg aggtggcaa 
      catacctca aacacttga ccaattact tgaaagaca 
      gtgggaaca aggatctca atttttaat ggaacaagc 
      ccaacattt tgatattgc atccttgct gtcttgtct 
      ccaccaact acgtgctga aaatttgag gctggaaga 
      acacgtcag ttcgttact tcaaagaag agatcattt 
      attggctgt caatgctag gttggctat tccattgac 
      tggccaacc cgcaacccc tcagtcttc ccatgctca 
      tacagtgaa taacataca ccctgctgt gggggccag 
      ccacgggga cgtgccccg ttcagggcg ccacagcgg 
      caacggtgc caactaccc cgcgcatcc ccaaggttc 
      acaacaccc aagtgtagc cccagttga aaatggttt 
      agcttcaga tccctccca accctatcc ctcatctca 
      ttcgcatcg aagtccagc aacaagtca gtcccaacc 
      cagtccatc ggttagaca ttcgccggc tggttcagg 
      gtatcccaa tagtggtct gcttcggca aaatgcaga 
      cactagcgg acgctctgc gcctagacc tcaaccgta 
      tccgttata tcaggggca ggggccttc gtcgcaaca 
      acagatggt aattagaag agactcaga tatatacca 
      tcagtcaac atcgaaacc tagctgaga gtatgtgga 
      tccgtcgtt tagcataac gagacttct agacttgca 
      ttgacaaat cactcaatt cataatttg ttgattatc 
      ctcgatcat tgtacagtg aaagtattt tcacaagtt 
      ggttttacg taaagactg aaaaacgag ttgtgattc 
      gtttttcag actttatag acaaaacag ccgtattca 
      tcatgaacg ttggaattg tcgaattgt aagaattga 
      aagttttta tttattgtt agagatgta atatgatct 
      ccgttttaa ccgtactta atatttaaa tatgtccag 
      gcatatcct gtcgactaa tatattata atacacgta 
      ttccatgta attttaact tttactaac tgcttataa 
      ttaaagaag taatgttta aaatattgg cgagtttta 
      tgacgatta gtaaaattt tcacgtgat tattatatt 
      atgtacttc tttgattgt atgcaattt taagtggaa 
      gaacaattt ttatttgat tctacacac catttacaa 
      aatcatgca aatttatct ctttatact ttttatcgt 
      tatagtttt attgtgtaa aactgtcag cgggtcggt 
      aatttttgc ccgaaaaga ctcctttga ttggtggtt 
      aattgtatt aaagcttta aaaatatca acatgattg 
      tgttcgatt tgagtgagt atggcgtat tttcgcaat 
      tttacattt atttagggt aatactttt ttgtttagt 
      tgtttcatt tattgtagt tattacagt ctcactgcc 
      actgcaata tgctcaatt atgtttcta ttgcctcta 
      gtcagattt tataacaga aaaaaccga cgtttgttg 
      acggtaagg ttgtttaat taggtgcat gcgaaggtt 
      ttcatggtt aaaaatgcg tgaagctta aaattttgc 
      taaatgtac aatgttaga gtgaaaagt atagtttta 
      gttgaatac tcagggata ttactagaa cacattgta 
      tcacaaagc tggttcata aacgaagtc tttaatagt 
      cttaagtta cgcattttt atgccagta acaatgcca 
      tattagtta attattcat gtaaatact aatagcgta 
      atttagtga ctttattct gtatatttg ttagtttag 
      caatgaaca cgcaattca tttagtttt cttgttgta 
      caaaccatt gtcgaaaat agtaatagt tttataaac 
      aagaaacgg aaatatccc tattttttt gcaaatatt 
      tttgggatt ttttgtact tttataaat aataagatt 
      tttaggag
    • >TC013701 RB
      agtcaaacc aaaatataa caaaaaaca aaatggttg 
      ctctataaa agaatgtgt ttaaaatgc atttttatg 
      caaaagtac gtgcattgt ggctcattt acgtgattt 
      actaaattg atattaggt aaacaccgg ctgcgagga 
      tgaacaaca aaacggtct agaaatgaa tgtgaacgt 
      attgcattg gagatgtgg ggtgggatt gtcaaatcg 
      ctaatttag caaacttcg cttctttgc attatgagg 
      gaaatggta ggaggttgt tgcgtttgt tctgacgat 
      agaggatgg tctgaaaat ccacttgtg tactgcgat 
      ggacaatct tgcactgtt gctgttcat caagcctgc 
      tatggtata gttactgtt cccactggt ccgtggtat 
      tgtcgcaaa tgcgagagt caagaaaga tctgttaaa 
      gtgaaatgc gaattgtgt cccagcaaa catggcgct 
      ctcaaacgc accgacaat tcaggttgg gctcacgtt 
      gtctgtgct ctttacatt ccagaagtg cgttttggc 
      aatgtcaca acgatggaa ccgattcag cttcaatta 
      attccaacg gaacgtttt aataagacg tgttacata 
      tgtgaagaa aagggaaaa gcttcgagt gcaacggtt 
      ggtgcatgt atgcaatgt aataaggcc ggttgcaag 
      cagcagttt catgtgact tgtgcacag agtttgggt 
      ttactttgc gaagaagct ggaaactat ttagacaac 
      gtcaaatat tgtggctat tgccaacac cattacagt 
      aaattgaag aaaggtggt aacgtgaaa acaatccct 
      ccttataag cccgtatcc acggaaaat cacctctcc 
      gactcgaat tcggagaag gaagcggaa aacaacgct 
      tcgacgaaa tcgtcgact tcttcatct caactgtca 
      tcatccaaa cgcaagacg agttcgagc agcaaagcc 
      tcctcgtct aagacgagt tcgagcacg aaaagctcg 
      tcgagttcg tcgtcttct catcagaaa accgtccat 
      aattcgagt aaaagttca gataaactg aaagtttcg 
      agttctagt gttaggagc agtgccaaa agtgcggaa 
      gacaacagt gctagtgtt gagagtggt aacgcggac 
      agcaaaacg gagtcgaga gacaacaaa gttgactta 
      gatcaaagc aaaacggac gttaaagat tcgaagagt 
      tcgaagaaa cgtaagaat aattcgagg acgccaacg 
      ccggttgcg ttagtgtcc agtgaaact gttagtgtt 
      gttaataca tccagcgtc gtggcggag aatttgggc 
      ggcacggcg tcggcgggc gagcggaaa ggggtggtc 
      ggagagccg gcggagaag ccaaaaaag atcaaggtg 
      gagacaacg cagtcaatt ttaacaaac cagccaatc 
      gttgcctta acttcaata tcaacgatt aaaaccata 
      tccaactct tcgataacg tcgtcattg aacgacaac 
      gtcgagacg gccccgtca ccatcacta cctccacaa 
      tccataatt cagtcaggt ccacctcac agtgtcaat 
      tctctagtg gtttccgtg cctctgact agtactagc 
      ttaagcccc catccccaa atcatcaac aaccaagcg 
      agtgtcgta acacccaca cctatcata cattcagaa 
      aggagtgtc aatagtcca gccgacaga gacacgtca 
      aataccaca ccagtaaac atgactggt ggtagtagt 
      aaccagggg agttcgata attacctca gaaatgagt 
      ggaggtggc ctaaaaatt acctacgag aagcaggat 
      gcggtgacc atggaagtg gatatggag ttggaagtt 
      tctccgtcg aatcaagac ttggcttcg aaaagatct 
      cattcagtg gaaaaatcc gagaaaggt ggtcgggga 
      aagaaacga aattcggga ggagcgaat gggattatt 
      aatcagttg aataatacg tcgatttct tcgtcgacg 
      gtgaaacgt tcgagtaga tctcagaat tcgacaccg 
      ccacagccg ggggccttt gccagtata agtcagtcg 
      cagattaag gattcacct cccagtagt cccagctcc 
      gaaagtcag agtgctgcg tcggtgcag aatggtcga 
      gggaagtcg aaatcgagg aaaagtgcg ccttcgtcg 
      cttgcaagg tcgcagtcg ccgaaagat atgaaagat 
      attaaattt tttcagaac ggggttaca gccccgcac 
      atgttgggt aatcagtta aaccccaat tccaacatg 
      gcacaaaaa atgtccgat catctaaat tccgaatta 
      gaagcccac agtattttc agtgcaagt gataattca 
      acaatgatg ggtccccaa ctccatcat cgagtaatc 
      gcttcggca agggcgagc agtgcaggt ggttcgagt 
      actgcttca gcaggtagc ggtttttca tcaatgtta 
      ggaggtgga ggtggcaac atacctcaa acacttgac 
      caattactt gaaagacag tgggaacaa ggatctcaa 
      tttttaatg gaacaagcc caacatttt gatattgca 
      tccttgctg tcttgtctc caccaacta cgtgctgaa 
      aatttgagg ctggaagaa cacgtcagt tcgttactt 
      caaagaaga gatcattta ttggctgtc aatgctagg 
      ttggctatt ccattgact ggccaaccc gcaacccct 
      cagtcttcc catgctcat acagtgaat aacatacac 
      cctgctgtg ggggccagc cacggggac gtgccccgt 
      tcagggcgc cacagcggc aacggtgcc aactacccc 
      gcgcatccc caaggttca caacaccca agtgtagcc 
      ccagttgaa aatggttta gcttcagat ccctcccaa 
      ccctatccc tcatctcat tcgcatcga agtccagca 
      acaagtcag tcccaaccc agtccatcg gttagacat 
      tcgccggct ggttcaggg tatcccaat agtggtctg 
      cttcggcaa aatgcagac actagcgga cgctctgcg 
      cctagacct caaccgtat ccgttatat caggggcag 
      gggccttcg tcgcaacaa caggtattt tgactgtta 
      gaagttgtt gcactgttt gtttcattt cat