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Gene information
General information
TC013697 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 4743923..4760715
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013697
    iBeetle screen iB_02187
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013697 RA
      MHSGGSDDV QRSIELLDR VLSEFDDVE NANQVQNIQ 
      QMTTSTSTP QLRQVGSNT TTGNVSSTP DDESPSLGH 
      QSEDDGYMS MNGRKPKFN LAFKPLAEV PVHHENASQ 
      SPTIMHPGD PDDFPPPPE EAQRLIATL LPKVSPAHR 
      PKTSTNSSR NRQFPLTQL PPRVETIPP PHKFQNNEY 
      TPIINGLPD PIHTATQTT LPKTRHQRP YGWENNDFT 
      LNPPPPPPG YRFGSLPFQ HGIYPITSP TRVPPRTIP 
      TALERHREV KRRGSEDNL SSDLEGSAL RPLSELADD 
      EHFSDDSLE EMLPPPPPV TNKRASIAW EVPLDDDAL 
      LTPGSTKVI GRRRRKSGS HSSTSSIPN RLKELEDWP 
      DPPPCTTED EVTSPFSDS DDHVILPPE LNSDHLSGT 
      STYIVRRGK KDRKALVNV GSSLNSNSS LNSRLSSDL 
      SIPALNSRL SRELNTPSS RYSVDLSTP HSRLSQELN 
      SPKSRYSLD LNNSRHLSQ ELSSSPSRL SADLSSSRS 
      STPSKQGNE FKKHSTTFD NIKSLIKEG RIENYNTPP 
      VECTSDLNP NIPPIVRVV SLPSLNAEE YAPRRELDV 
      TVEEEEDGE TSQDSAEIS PLRQIEQNI SELLERRDI 
      EVIQKDEKC LLNGVESLT KKEKRKAPP RPKKGHDLQ 
      KSSSHNEIQ RSEDYYRQL LIEAGNPFP KRNGIQKSE 
      SAKEMLIAH RESRPLPTS TSADFPVRV EVLQHDFPP 
      LPPSPVEED DEYSEIVHP TQNRADTLP ASSEPPVVP 
      PHRDPVSNS LKTRSMDAN YSRGRRSNF YSSSSSRRT 
      LPMELQESK RKPFQKQEE HHLQTSCSL PETPIFARG 
      CDIPRTPHR RAPDVPGGT APRQSNTIG SLNTIGNMS 
      TGGYRRGMS NPGLEQAFR GAELLRLAG GPGRGWYPK 
      QRHRRPASI EHLDRMASG HNPWEPNRK PLTLPPNLT 
      PKFFQRSPR DALRRVTSL LIRKGNNGK EPRKEKEIV 
      SPTIHENAN GEEVQKKKG FFKSFWKRS RHYSLEQQ
    CDS sequence
    • >TC013697 RA
      atgcactct gggggaagc gacgacgtg cagcggtcc 
      atcgagcta ctcgaccgg gtgttgtcg gagttcgat 
      gacgtggaa aatgcgaac caagttcag aatatacaa 
      caaatgacg acgtcgacg tcgacgccg cagttgaga 
      caagtcggc agtaacaca accaccggc aacgtatca 
      tccaccccg gacgacgag agcccttct ctcggtcat 
      cagtcagag gatgacggt tacatgagc atgaacggc 
      cgcaaacct aagttcaac ttagccttc aagccgttg 
      gccgaagtg cctgtccat cacgagaat gcctcgcaa 
      tctcccaca attatgcat ccgggagat ccagacgac 
      ttcccgccg cctccggag gaggcgcaa cggctcatc 
      gccacactg ttgcctaaa gtttccccg gcacatagg 
      ccgaaaacc agcacgaat tcgagtcgg aaccgtcag 
      ttccccctg actcagttg ccgcctcga gtagaaacg 
      ataccaccg ccgcacaag ttccaaaac aatgagtat 
      acgccaata atcaacggt ctacccgat ccaattcat 
      actgccact caaacgact ctgcctaaa acaaggcat 
      caacgtcca tacggatgg gaaaacaac gattttacg 
      ttaaatccc ccgcctcca cccccggga taccgcttc 
      ggtagtctc ccgtttcaa catgggatt tatcccatc 
      acctccccg acgcgtgtt ccgccgagg acgatccca 
      actgctttg gaacgtcat cgagaagtc aagagacgt 
      ggcagcgaa gacaatctc tccagcgac ctagaaggg 
      tcggccttg cggcccttg agcgaattg gcggacgat 
      gagcacttc tcagacgat tccctcgaa gaaatgctg 
      ccgcccccg ccgcccgtg accaataaa cgcgccagc 
      atcgcctgg gaggtgcct ctagacgac gatgccctc 
      cttactccg ggtagcacc aaagtcatc ggtagaaga 
      cgaaggaaa tccggatcg cactctagc actagttca 
      ataccgaat agattaaaa gaattagaa gactggccc 
      gatccgccc ccttgtacc acagaggac gaagtaacg 
      tcaccgttt tccgattca gacgaccac gttatcctt 
      ccaccggaa ctaaactcg gatcattta tcagggacg 
      agcacttat atcgtacgg agaggcaaa aaggaccgg 
      aaggcctta gtcaacgtc ggaagttcg ttaaattcg 
      aattcttca ctcaattcg agattaagc tcagatttg 
      agcatccct gcattaaac agtcgccta agtcgggaa 
      ttgaacaca ccaagctct agatatagc gtagatttg 
      agcacccca cattcgaga ctcagtcaa gaactcaac 
      tctcccaaa tctaggtat agcttagat ttgaataat 
      tcgagacac ttgagtcag gaactgagc agttcaccg 
      tcgaggctc agtgccgac ctctccagt tctagatcg 
      agcactccc agcaagcaa ggaaacgaa tttaaaaag 
      cacagcacc acgttcgat aacattaaa tcgttgatt 
      aaagaagga agaatagaa aactacaac accccccct 
      gttgaatgc acctcagac ctgaacccc aatattccc 
      ccaattgta cgagtagtg tcactgcct agtttaaac 
      gcagaagag tacgcaccc cgaagggag ctagacgtg 
      acggtggaa gaggaggaa gacggtgag acgtcgcaa 
      gacagcgcc gaaatctcg cctctgcgc caaatcgag 
      caaaacatt agcgagtta ctcgagcga cgcgacatc 
      gaagtgatc caaaaggac gagaaatgc ctcttgaac 
      ggtgtcgag tcgttgacg aaaaaagag aagcggaaa 
      gctccaccg aggccgaag aaaggccac gatctccag 
      aagtcaagt agtcacaat gaaattcag cgctcggaa 
      gactactac cgtcaatta ctgatcgag gccggaaac 
      ccgttcccg aaacgcaac gggatacaa aagtcggag 
      agtgccaaa gaaatgcta atcgcgcac cgggaaagc 
      aggcctttg cccacgtcc acgtcggct gatttcccg 
      gtgcgagtg gaggtactc cagcacgac ttcccgcca 
      ctgccgccc tcgccggtg gaggaagac gatgagtac 
      tccgaaatc gtccatccg acacaaaat cgggccgat 
      accctgccc gccagcagc gagccacct gtagtgcct 
      ccacaccgc gatcccgtc tcgaacagc ctgaaaacg 
      cgttcgatg gacgcgaat tacagtcga ggaagacgc 
      agcaacttc tacagctcg tcaagcagt cgacgcaca 
      ttgcccatg gaattgcaa gagtcgaag cggaagccg 
      ttccagaag caagaggag caccacctg cagacgtcg 
      tgcagtctg ccggagacg ccgatcttc gccaggggt 
      tgtgacatt ccacgaacg cctcaccgg agggcgccc 
      gatgtgccc gggggcaca gcaccaaga cagagcaac 
      acgatcggc agtctgaac acgataggg aacatgtcc 
      acgggaggg tacaggaga ggcatgtcg aatccgggg 
      ctggaacag gcgttcaga ggagcggag ctgttgagg 
      ttggcgggc gggcctggc aggggctgg tacccgaag 
      cagaggcac agaaggccc gcgtccatt gaacacttg 
      gacaggatg gcatcgggg cacaacccc tgggagccc 
      aacaggaaa cctctcact ctcccgccg aatctaacg 
      cccaagttc ttccaaaga tcgcccagg gatgcgctg 
      agaagagtt accagtctg cttataagg aaaggaaac 
      aatggaaaa gaaccaagg aaagagaag gaaattgtg 
      tcgcccacg atccacgaa aatgctaat ggagaggaa 
      gtgcagaag aaaaaggga ttcttcaaa agcttctgg 
      aagcggtca cggcattac tctttagaa cagcaataa
    mRNA sequence
    • >TC013697 RA
      attggctag taaaactgt tctatttgc aagaataac 
      aggattggt gagctaatg cgagcactt aaaagccac 
      gacatggcc attatttgg ttcaattaa agctgtggc 
      tttccgagg aaaacagat tctgctact tgtaatgcg 
      atttgttaa atatgacaa tgtagcacc aagtctgat 
      tcgggtcct attattaga aaatgcact ctgggggaa 
      gcgacgacg tgcagcggt ccatcgagc tactcgacc 
      gggtgttgt cggagttcg atgacgtgg aaaatgcga 
      accaagttc agaatatac aacaaatga cgacgtcga 
      cgtcgacgc cgcagttga gacaagtcg gcagtaaca 
      caaccaccg gcaacgtat catccaccc cggacgacg 
      agagccctt ctctcggtc atcagtcag aggatgacg 
      gttacatga gcatgaacg gccgcaaac ctaagttca 
      acttagcct tcaagccgt tggccgaag tgcctgtcc 
      atcacgaga atgcctcgc aatctccca caattatgc 
      atccgggag atccagacg acttcccgc cgcctccgg 
      aggaggcgc aacggctca tcgccacac tgttgccta 
      aagtttccc cggcacata ggccgaaaa ccagcacga 
      attcgagtc ggaaccgtc agttccccc tgactcagt 
      tgccgcctc gagtagaaa cgataccac cgccgcaca 
      agttccaaa acaatgagt atacgccaa taatcaacg 
      gtctacccg atccaattc atactgcca ctcaaacga 
      ctctgccta aaacaaggc atcaacgtc catacggat 
      gggaaaaca acgatttta cgttaaatc ccccgcctc 
      cacccccgg gataccgct tcggtagtc tcccgtttc 
      aacatggga tttatccca tcacctccc cgacgcgtg 
      ttccgccga ggacgatcc caactgctt tggaacgtc 
      atcgagaag tcaagagac gtggcagcg aagacaatc 
      tctccagcg acctagaag ggtcggcct tgcggccct 
      tgagcgaat tggcggacg atgagcact tctcagacg 
      attccctcg aagaaatgc tgccgcccc cgccgcccg 
      tgaccaata aacgcgcca gcatcgcct gggaggtgc 
      ctctagacg acgatgccc tccttactc cgggtagca 
      ccaaagtca tcggtagaa gacgaagga aatccggat 
      cgcactcta gcactagtt caataccga atagattaa 
      aagaattag aagactggc ccgatccgc ccccttgta 
      ccacagagg acgaagtaa cgtcaccgt tttccgatt 
      cagacgacc acgttatcc ttccaccgg aactaaact 
      cggatcatt tatcaggga cgagcactt atatcgtac 
      ggagaggca aaaaggacc ggaaggcct tagtcaacg 
      tcggaagtt cgttaaatt cgaattctt cactcaatt 
      cgagattaa gctcagatt tgagcatcc ctgcattaa 
      acagtcgcc taagtcggg aattgaaca caccaagct 
      ctagatata gcgtagatt tgagcaccc cacattcga 
      gactcagtc aagaactca actctccca aatctaggt 
      atagcttag atttgaata attcgagac acttgagtc 
      aggaactga gcagttcac cgtcgaggc tcagtgccg 
      acctctcca gttctagat cgagcactc ccagcaagc 
      aaggaaacg aatttaaaa agcacagca ccacgttcg 
      ataacatta aatcgttga ttaaagaag gaagaatag 
      aaaactaca acacccccc ctgttgaat gcacctcag 
      acctgaacc ccaatattc ccccaattg tacgagtag 
      tgtcactgc ctagtttaa acgcagaag agtacgcac 
      cccgaaggg agctagacg tgacggtgg aagaggagg 
      aagacggtg agacgtcgc aagacagcg ccgaaatct 
      cgcctctgc gccaaatcg agcaaaaca ttagcgagt 
      tactcgagc gacgcgaca tcgaagtga tccaaaagg 
      acgagaaat gcctcttga acggtgtcg agtcgttga 
      cgaaaaaag agaagcgga aagctccac cgaggccga 
      agaaaggcc acgatctcc agaagtcaa gtagtcaca 
      atgaaattc agcgctcgg aagactact accgtcaat 
      tactgatcg aggccggaa acccgttcc cgaaacgca 
      acgggatac aaaagtcgg agagtgcca aagaaatgc 
      taatcgcgc accgggaaa gcaggcctt tgcccacgt 
      ccacgtcgg ctgatttcc cggtgcgag tggaggtac 
      tccagcacg acttcccgc cactgccgc cctcgccgg 
      tggaggaag acgatgagt actccgaaa tcgtccatc 
      cgacacaaa atcgggccg ataccctgc ccgccagca 
      gcgagccac ctgtagtgc ctccacacc gcgatcccg 
      tctcgaaca gcctgaaaa cgcgttcga tggacgcga 
      attacagtc gaggaagac gcagcaact tctacagct 
      cgtcaagca gtcgacgca cattgccca tggaattgc 
      aagagtcga agcggaagc cgttccaga agcaagagg 
      agcaccacc tgcagacgt cgtgcagtc tgccggaga 
      cgccgatct tcgccaggg gttgtgaca ttccacgaa 
      cgcctcacc ggagggcgc ccgatgtgc ccgggggca 
      cagcaccaa gacagagca acacgatcg gcagtctga 
      acacgatag ggaacatgt ccacgggag ggtacagga 
      gaggcatgt cgaatccgg ggctggaac aggcgttca 
      gaggagcgg agctgttga ggttggcgg gcgggcctg 
      gcaggggct ggtacccga agcagaggc acagaaggc 
      ccgcgtcca ttgaacact tggacagga tggcatcgg 
      ggcacaacc cctgggagc ccaacagga aacctctca 
      ctctcccgc cgaatctaa cgcccaagt tcttccaaa 
      gatcgccca gggatgcgc tgagaagag ttaccagtc 
      tgcttataa ggaaaggaa acaatggaa aagaaccaa 
      ggaaagaga aggaaattg tgtcgccca cgatccacg 
      aaaatgcta atggagagg aagtgcaga agaaaaagg 
      gattcttca aaagcttct ggaagcggt cacggcatt 
      actctttag aacagcaat aaagtggtc ccctttccc 
      acgtataga aacaaattt atctgatta gtttttgtg 
      tacatttta tataacaat aatttaata aatctaacc 
      gattagtat tactatttg tatcgctaa caagaaaag 
      agcacttga tcctaatat tacatagtt gtagatatt 
      ttaactaat ttacgatta cgacattgt gtaacaagg 
      aaatttgag aacttttcg tctgtaaca gtttatact 
      ttttgcggt ttttgtttt atgttttgc tttcaaact 
      gtataattg tacattagt tttatttat aaattttat 
      ttctatctg tgtgttttt gatttaata aagtttatt 
      taacgat