| General information | |||
| TC013697 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 5779131..5795923 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013697 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02187 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC013697 RA
MHSGGSDDV QRSIELLDR VLSEFDDVE NANQVQNIQ QMTTSTSTP QLRQVGSNT TTGNVSSTP DDESPSLGH QSEDDGYMS MNGRKPKFN LAFKPLAEV PVHHENASQ SPTIMHPGD PDDFPPPPE EAQRLIATL LPKVSPAHR PKTSTNSSR NRQFPLTQL PPRVETIPP PHKFQNNEY TPIINGLPD PIHTATQTT LPKTRHQRP YGWENNDFT LNPPPPPPG YRFGSLPFQ HGIYPITSP TRVPPRTIP TALERHREV KRRGSEDNL SSDLEGSAL RPLSELADD EHFSDDSLE EMLPPPPPV TNKRASIAW EVPLDDDAL LTPGSTKVI GRRRRKSGS HSSTSSIPN RLKELEDWP DPPPCTTED EVTSPFSDS DDHVILPPE LNSDHLSGT STYIVRRGK KDRKALVNV GSSLNSNSS LNSRLSSDL SIPALNSRL SRELNTPSS RYSVDLSTP HSRLSQELN SPKSRYSLD LNNSRHLSQ ELSSSPSRL SADLSSSRS STPSKQGNE FKKHSTTFD NIKSLIKEG RIENYNTPP VECTSDLNP NIPPIVRVV SLPSLNAEE YAPRRELDV TVEEEEDGE TSQDSAEIS PLRQIEQNI SELLERRDI EVIQKDEKC LLNGVESLT KKEKRKAPP RPKKGHDLQ KSSSHNEIQ RSEDYYRQL LIEAGNPFP KRNGIQKSE SAKEMLIAH RESRPLPTS TSADFPVRV EVLQHDFPP LPPSPVEED DEYSEIVHP TQNRADTLP ASSEPPVVP PHRDPVSNS LKTRSMDAN YSRGRRSNF YSSSSSRRT LPMELQESK RKPFQKQEE HHLQTSCSL PETPIFARG CDIPRTPHR RAPDVPGGT APRQSNTIG SLNTIGNMS TGGYRRGMS NPGLEQAFR GAELLRLAG GPGRGWYPK QRHRRPASI EHLDRMASG HNPWEPNRK PLTLPPNLT PKFFQRSPR DALRRVTSL LIRKGNNGK EPRKEKEIV SPTIHENAN GEEVQKKKG FFKSFWKRS RHYSLEQQ
- >TC013697 RA
atgcactct gggggaagc gacgacgtg cagcggtcc atcgagcta ctcgaccgg gtgttgtcg gagttcgat gacgtggaa aatgcgaac caagttcag aatatacaa caaatgacg acgtcgacg tcgacgccg cagttgaga caagtcggc agtaacaca accaccggc aacgtatca tccaccccg gacgacgag agcccttct ctcggtcat cagtcagag gatgacggt tacatgagc atgaacggc cgcaaacct aagttcaac ttagccttc aagccgttg gccgaagtg cctgtccat cacgagaat gcctcgcaa tctcccaca attatgcat ccgggagat ccagacgac ttcccgccg cctccggag gaggcgcaa cggctcatc gccacactg ttgcctaaa gtttccccg gcacatagg ccgaaaacc agcacgaat tcgagtcgg aaccgtcag ttccccctg actcagttg ccgcctcga gtagaaacg ataccaccg ccgcacaag ttccaaaac aatgagtat acgccaata atcaacggt ctacccgat ccaattcat actgccact caaacgact ctgcctaaa acaaggcat caacgtcca tacggatgg gaaaacaac gattttacg ttaaatccc ccgcctcca cccccggga taccgcttc ggtagtctc ccgtttcaa catgggatt tatcccatc acctccccg acgcgtgtt ccgccgagg acgatccca actgctttg gaacgtcat cgagaagtc aagagacgt ggcagcgaa gacaatctc tccagcgac ctagaaggg tcggccttg cggcccttg agcgaattg gcggacgat gagcacttc tcagacgat tccctcgaa gaaatgctg ccgcccccg ccgcccgtg accaataaa cgcgccagc atcgcctgg gaggtgcct ctagacgac gatgccctc cttactccg ggtagcacc aaagtcatc ggtagaaga cgaaggaaa tccggatcg cactctagc actagttca ataccgaat agattaaaa gaattagaa gactggccc gatccgccc ccttgtacc acagaggac gaagtaacg tcaccgttt tccgattca gacgaccac gttatcctt ccaccggaa ctaaactcg gatcattta tcagggacg agcacttat atcgtacgg agaggcaaa aaggaccgg aaggcctta gtcaacgtc ggaagttcg ttaaattcg aattcttca ctcaattcg agattaagc tcagatttg agcatccct gcattaaac agtcgccta agtcgggaa ttgaacaca ccaagctct agatatagc gtagatttg agcacccca cattcgaga ctcagtcaa gaactcaac tctcccaaa tctaggtat agcttagat ttgaataat tcgagacac ttgagtcag gaactgagc agttcaccg tcgaggctc agtgccgac ctctccagt tctagatcg agcactccc agcaagcaa ggaaacgaa tttaaaaag cacagcacc acgttcgat aacattaaa tcgttgatt aaagaagga agaatagaa aactacaac accccccct gttgaatgc acctcagac ctgaacccc aatattccc ccaattgta cgagtagtg tcactgcct agtttaaac gcagaagag tacgcaccc cgaagggag ctagacgtg acggtggaa gaggaggaa gacggtgag acgtcgcaa gacagcgcc gaaatctcg cctctgcgc caaatcgag caaaacatt agcgagtta ctcgagcga cgcgacatc gaagtgatc caaaaggac gagaaatgc ctcttgaac ggtgtcgag tcgttgacg aaaaaagag aagcggaaa gctccaccg aggccgaag aaaggccac gatctccag aagtcaagt agtcacaat gaaattcag cgctcggaa gactactac cgtcaatta ctgatcgag gccggaaac ccgttcccg aaacgcaac gggatacaa aagtcggag agtgccaaa gaaatgcta atcgcgcac cgggaaagc aggcctttg cccacgtcc acgtcggct gatttcccg gtgcgagtg gaggtactc cagcacgac ttcccgcca ctgccgccc tcgccggtg gaggaagac gatgagtac tccgaaatc gtccatccg acacaaaat cgggccgat accctgccc gccagcagc gagccacct gtagtgcct ccacaccgc gatcccgtc tcgaacagc ctgaaaacg cgttcgatg gacgcgaat tacagtcga ggaagacgc agcaacttc tacagctcg tcaagcagt cgacgcaca ttgcccatg gaattgcaa gagtcgaag cggaagccg ttccagaag caagaggag caccacctg cagacgtcg tgcagtctg ccggagacg ccgatcttc gccaggggt tgtgacatt ccacgaacg cctcaccgg agggcgccc gatgtgccc gggggcaca gcaccaaga cagagcaac acgatcggc agtctgaac acgataggg aacatgtcc acgggaggg tacaggaga ggcatgtcg aatccgggg ctggaacag gcgttcaga ggagcggag ctgttgagg ttggcgggc gggcctggc aggggctgg tacccgaag cagaggcac agaaggccc gcgtccatt gaacacttg gacaggatg gcatcgggg cacaacccc tgggagccc aacaggaaa cctctcact ctcccgccg aatctaacg cccaagttc ttccaaaga tcgcccagg gatgcgctg agaagagtt accagtctg cttataagg aaaggaaac aatggaaaa gaaccaagg aaagagaag gaaattgtg tcgcccacg atccacgaa aatgctaat ggagaggaa gtgcagaag aaaaaggga ttcttcaaa agcttctgg aagcggtca cggcattac tctttagaa cagcaataa
- >TC013697 RA
attggctag taaaactgt tctatttgc aagaataac aggattggt gagctaatg cgagcactt aaaagccac gacatggcc attatttgg ttcaattaa agctgtggc tttccgagg aaaacagat tctgctact tgtaatgcg atttgttaa atatgacaa tgtagcacc aagtctgat tcgggtcct attattaga aaatgcact ctgggggaa gcgacgacg tgcagcggt ccatcgagc tactcgacc gggtgttgt cggagttcg atgacgtgg aaaatgcga accaagttc agaatatac aacaaatga cgacgtcga cgtcgacgc cgcagttga gacaagtcg gcagtaaca caaccaccg gcaacgtat catccaccc cggacgacg agagccctt ctctcggtc atcagtcag aggatgacg gttacatga gcatgaacg gccgcaaac ctaagttca acttagcct tcaagccgt tggccgaag tgcctgtcc atcacgaga atgcctcgc aatctccca caattatgc atccgggag atccagacg acttcccgc cgcctccgg aggaggcgc aacggctca tcgccacac tgttgccta aagtttccc cggcacata ggccgaaaa ccagcacga attcgagtc ggaaccgtc agttccccc tgactcagt tgccgcctc gagtagaaa cgataccac cgccgcaca agttccaaa acaatgagt atacgccaa taatcaacg gtctacccg atccaattc atactgcca ctcaaacga ctctgccta aaacaaggc atcaacgtc catacggat gggaaaaca acgatttta cgttaaatc ccccgcctc cacccccgg gataccgct tcggtagtc tcccgtttc aacatggga tttatccca tcacctccc cgacgcgtg ttccgccga ggacgatcc caactgctt tggaacgtc atcgagaag tcaagagac gtggcagcg aagacaatc tctccagcg acctagaag ggtcggcct tgcggccct tgagcgaat tggcggacg atgagcact tctcagacg attccctcg aagaaatgc tgccgcccc cgccgcccg tgaccaata aacgcgcca gcatcgcct gggaggtgc ctctagacg acgatgccc tccttactc cgggtagca ccaaagtca tcggtagaa gacgaagga aatccggat cgcactcta gcactagtt caataccga atagattaa aagaattag aagactggc ccgatccgc ccccttgta ccacagagg acgaagtaa cgtcaccgt tttccgatt cagacgacc acgttatcc ttccaccgg aactaaact cggatcatt tatcaggga cgagcactt atatcgtac ggagaggca aaaaggacc ggaaggcct tagtcaacg tcggaagtt cgttaaatt cgaattctt cactcaatt cgagattaa gctcagatt tgagcatcc ctgcattaa acagtcgcc taagtcggg aattgaaca caccaagct ctagatata gcgtagatt tgagcaccc cacattcga gactcagtc aagaactca actctccca aatctaggt atagcttag atttgaata attcgagac acttgagtc aggaactga gcagttcac cgtcgaggc tcagtgccg acctctcca gttctagat cgagcactc ccagcaagc aaggaaacg aatttaaaa agcacagca ccacgttcg ataacatta aatcgttga ttaaagaag gaagaatag aaaactaca acacccccc ctgttgaat gcacctcag acctgaacc ccaatattc ccccaattg tacgagtag tgtcactgc ctagtttaa acgcagaag agtacgcac cccgaaggg agctagacg tgacggtgg aagaggagg aagacggtg agacgtcgc aagacagcg ccgaaatct cgcctctgc gccaaatcg agcaaaaca ttagcgagt tactcgagc gacgcgaca tcgaagtga tccaaaagg acgagaaat gcctcttga acggtgtcg agtcgttga cgaaaaaag agaagcgga aagctccac cgaggccga agaaaggcc acgatctcc agaagtcaa gtagtcaca atgaaattc agcgctcgg aagactact accgtcaat tactgatcg aggccggaa acccgttcc cgaaacgca acgggatac aaaagtcgg agagtgcca aagaaatgc taatcgcgc accgggaaa gcaggcctt tgcccacgt ccacgtcgg ctgatttcc cggtgcgag tggaggtac tccagcacg acttcccgc cactgccgc cctcgccgg tggaggaag acgatgagt actccgaaa tcgtccatc cgacacaaa atcgggccg ataccctgc ccgccagca gcgagccac ctgtagtgc ctccacacc gcgatcccg tctcgaaca gcctgaaaa cgcgttcga tggacgcga attacagtc gaggaagac gcagcaact tctacagct cgtcaagca gtcgacgca cattgccca tggaattgc aagagtcga agcggaagc cgttccaga agcaagagg agcaccacc tgcagacgt cgtgcagtc tgccggaga cgccgatct tcgccaggg gttgtgaca ttccacgaa cgcctcacc ggagggcgc ccgatgtgc ccgggggca cagcaccaa gacagagca acacgatcg gcagtctga acacgatag ggaacatgt ccacgggag ggtacagga gaggcatgt cgaatccgg ggctggaac aggcgttca gaggagcgg agctgttga ggttggcgg gcgggcctg gcaggggct ggtacccga agcagaggc acagaaggc ccgcgtcca ttgaacact tggacagga tggcatcgg ggcacaacc cctgggagc ccaacagga aacctctca ctctcccgc cgaatctaa cgcccaagt tcttccaaa gatcgccca gggatgcgc tgagaagag ttaccagtc tgcttataa ggaaaggaa acaatggaa aagaaccaa ggaaagaga aggaaattg tgtcgccca cgatccacg aaaatgcta atggagagg aagtgcaga agaaaaagg gattcttca aaagcttct ggaagcggt cacggcatt actctttag aacagcaat aaagtggtc ccctttccc acgtataga aacaaattt atctgatta gtttttgtg tacatttta tataacaat aatttaata aatctaacc gattagtat tactatttg tatcgctaa caagaaaag agcacttga tcctaatat tacatagtt gtagatatt ttaactaat ttacgatta cgacattgt gtaacaagg aaatttgag aacttttcg tctgtaaca gtttatact ttttgcggt ttttgtttt atgttttgc tttcaaact gtataattg tacattagt tttatttat aaattttat ttctatctg tgtgttttt gatttaata aagtttatt taacgat
