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Gene information
General information
TC013692 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 4903326..4904574
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013692
    iBeetle screen iB_02186
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013692 RA
      MANSNEPDE TNLPTLFNQ GLDLYNSIC NSNEPTNSP 
      PVQSNVKKA MSLFEQATR LVSFAGIFS TNEGIEEVA 
      TNDLQYFLL PALLGSLSL KLTSGERKE IINVAEIYF 
      KDFLSRCND YGLSNYDFT EKKEQKKPQ TELEEITVS 
      VNTRANKIQ RYKEQKELK SKLADLKTN MENEHADEE 
      IKRDYFLTM LKSFIHEAV DELNSIEME KPILEYMAN 
      VKKDEKKPE PKRPVTPLK PIIITKDEV QKAVFGAGY 
      PSLPTMTVQ EFYDKRVKD GIFPDPTKP PTGPMSLQQ 
      AALAGVELN CEEKEAEEK EKIVESDDP EYIERMRRM 
      DEFKDEHRR GWGNRANRS
    CDS sequence
    • >TC013692 RA
      atggcaaat tctaatgaa cctgacgaa acgaacctt 
      cccacttta tttaaccaa ggtttggat ttatacaac 
      tcaatctgc aacagcaat gaacctaca aacagcccc 
      ccagtccag agcaacgta aaaaaggca atgagtctt 
      ttcgagcaa gccactcgt ttggtgtca tttgccgga 
      atttttagc accaatgaa gggattgaa gaagtggca 
      acaaacgac ctacagtat ttcctcctt ccagcactg 
      ctaggttcc ctaagttta aaattaacc agtggtgaa 
      cgtaaagaa atcataaac gttgccgaa atttacttt 
      aaggatttt ttgagcaga tgcaacgat tacggttta 
      agtaactac gactttacc gaaaagaag gaacaaaag 
      aagccacag actgaactg gaggaaatc acagtatcg 
      gtaaacacg agggcaaac aaaatacaa aggtacaaa 
      gaacaaaag gagttgaag tcgaaactc gccgattta 
      aaaacgaac atggaaaat gagcatgct gatgaagaa 
      atcaaaagg gactacttc ctaactatg ctcaagtcc 
      ttcatccat gaagccgta gacgaactg aacagcata 
      gaaatggaa aaaccaata ctggaatac atggctaat 
      gtgaaaaag gatgagaag aaaccggag ccaaaaaga 
      ccagtaacc ccactcaag cccataatt atcacaaaa 
      gatgaggtc cagaaggcg gtttttggc gccggatat 
      ccttccttg ccgacgatg acggttcaa gaattttat 
      gataagagg gtcaaagac gggattttt cctgaccct 
      actaaacca ccaactggt cccatgagc ctgcagcaa 
      gctgctttg gcaggggtg gagttaaac tgcgaagag 
      aaggaagca gaagaaaag gaaaaaatc gtcgagagt 
      gacgatcca gagtacatt gagagaatg cggaggatg 
      gatgagttt aaggacgaa cacaggagg gggtgggga 
      aatagggcg aataggagt tag
    mRNA sequence
    • >TC013692 RA
      ttttgacac ttcttcgtg acaggtgtt ttgtgtaca 
      atatttgtt atttataga atgatagct cacaatcta 
      aattagaaa aagtgaata tggcaaatt ctaatgaac 
      ctgacgaaa cgaaccttc ccactttat ttaaccaag 
      gtttggatt tatacaact caatctgca acagcaatg 
      aacctacaa acagccccc cagtccaga gcaacgtaa 
      aaaaggcaa tgagtcttt tcgagcaag ccactcgtt 
      tggtgtcat ttgccggaa tttttagca ccaatgaag 
      ggattgaag aagtggcaa caaacgacc tacagtatt 
      tcctccttc cagcactgc taggttccc taagtttaa 
      aattaacca gtggtgaac gtaaagaaa tcataaacg 
      ttgccgaaa tttacttta aggattttt tgagcagat 
      gcaacgatt acggtttaa gtaactacg actttaccg 
      aaaagaagg aacaaaaga agccacaga ctgaactgg 
      aggaaatca cagtatcgg taaacacga gggcaaaca 
      aaatacaaa ggtacaaag aacaaaagg agttgaagt 
      cgaaactcg ccgatttaa aaacgaaca tggaaaatg 
      agcatgctg atgaagaaa tcaaaaggg actacttcc 
      taactatgc tcaagtcct tcatccatg aagccgtag 
      acgaactga acagcatag aaatggaaa aaccaatac 
      tggaataca tggctaatg tgaaaaagg atgagaaga 
      aaccggagc caaaaagac cagtaaccc cactcaagc 
      ccataatta tcacaaaag atgaggtcc agaaggcgg 
      tttttggcg ccggatatc cttccttgc cgacgatga 
      cggttcaag aattttatg ataagaggg tcaaagacg 
      ggatttttc ctgacccta ctaaaccac caactggtc 
      ccatgagcc tgcagcaag ctgctttgg caggggtgg 
      agttaaact gcgaagaga aggaagcag aagaaaagg 
      aaaaaatcg tcgagagtg acgatccag agtacattg 
      agagaatgc ggaggatgg atgagttta aggacgaac 
      acaggaggg ggtggggaa atagggcga ataggagtt 
      aggcacgga gttagttat ttgtatttt tagaataaa 
      tgtctaaac tttggttgt gacctttca aataaacaa 
      ttttgcgtc gt