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Gene information
General information
TC013686 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 5018498..5024153
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013686
    iBeetle screen iB_02184
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Adult morphology
    adult bristle pattern irregular - Number of animals showing the phenotype: 4

    Comment: the depressions of the sensory bristles seem to be enlarged (diameter increased, maybe deeper)

    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 1
    tropharium size decreased - Number of animals showing the phenotype: 4

    tropharium shape irregular - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4
    tropharium posterior potentiallysize decreased - Number of animals showing the phenotype: 4

    Comment: the number of proocytes and the somatic plug seems to be reduced
    previtellogenic egg chamber potentially number decreased - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013686 RA
      MFGAKKSEK TKRDHSGRN LSQYGLFDV SADFNLDGG 
      LMDGGLDNE NDSDLEAEL AALTQGGGG KPKRPPRKK 
      VPDADLDAM VAESMKDIP SDEELSGDD DDPELLNEL 
      SNIAGDTPE KEEEDQPSP ARSPEGSSD IVKLLNDRL 
      KMYEEAEKN AKAAGESSR ARRFGRGLK TLKDLIKQA 
      NAGRPINND DIPPEVSTG VKKPPATDD TTDSPPQPS 
      RAAPPVPVQ SDPEPEPEP EKTPEETPQ VDPQLIHLL 
      TERKNQYKI AALKAKKSG DNATAISYI KIAKQFETV 
      IAAAQSGQP VDLSRMPGP PQEPVEKVE ENKTQNDSV 
      PQSDEPEAD ETLITASSV EEALEQRLA VYKKQEESA 
      KEQGNASKA RRMGRIVKQ YEQAIKAHK AGKPIPVDE 
      LPTPPGYAP IPVPGSEAP KPTPAAPEQ PPRPKTSPK 
      TQPSTSQST RISGNHVPN SRADKQLVL LLAKQKQFK 
      EAALAAKRK GEIDQAKEY LRTAKGFDP LIDAASCGL 
      PVDLSTLPV SPSAKSQLD NEYEMVMAD ECSEDDNSN 
      TDVIARLEN QLTKQLKMC LSTRDHHKA LGDVAGTNR 
      FERLALNVT KDLDIVRLA RRTPGAGVP KFHYENRDF 
      SIVKSFTEL GENDLELTI VRGISYTCS NPKEIDTYV 
      KFDFPYPQD EPFSQRTAT VKDTNSPEY NATYTIPIQ 
      RNARVCQRV FKRQSVKFE VYSKGGFFR GDTLIGTAT 
      VKLQPLETQ CELHDSFDL LEGRKKIGG KLEVRMRLR 
      HPIVNQQVE QLHEKWLVI DNV
    CDS sequence
    • >TC013686 RA
      atgtttggg gctaagaaa agcgagaaa acaaagcgg 
      gaccattcc gggaggaat ttgtcacag tatgggttg 
      tttgatgta tcagctgat tttaaccta gacgggggg 
      ctaatggac ggtggtctc gacaatgag aatgatagt 
      gatttggaa gccgaattg gcggcgttg actcaaggg 
      ggcggcggc aaacccaaa cgtccccca aggaagaag 
      gttcctgat gcggattta gacgcaatg gttgctgaa 
      agtatgaag gatatacct tcagacgag gagctgtca 
      ggggatgac gatgatcca gaactctta aatgagcta 
      agcaacatt gcgggagac acacccgaa aaagaagag 
      gaggatcaa ccatcgccg gcaaggtca cctgagggg 
      tccagcgac atagtgaag cttttaaat gaccgatta 
      aaaatgtac gaagaagca gaaaaaaac gctaaagct 
      gccggtgaa tcaagcagg gcaagacga tttggcaga 
      ggcttgaaa accctcaag gacttaata aaacaagcg 
      aacgccggc cgacccatt aacaacgac gacattcct 
      cccgaagtt agcactgga gtgaaaaaa cccccagcc 
      accgacgac acaaccgat tcgccgccg cagccctca 
      agggctgct cctccagtt cctgtacaa tctgacccc 
      gaacctgaa ccagaaccc gaaaaaacc cctgaagaa 
      acaccccaa gtagacccc caactaatc cacttgctt 
      acggaacga aaaaatcaa tacaaaatt gccgccctc 
      aaagccaaa aaatcaggc gataacgcc actgccatt 
      agttacata aaaatagcc aaacagttt gaaacggtc 
      atcgcagcg gcccaaagc ggccaacca gtcgactta 
      tctcgcatg ccaggaccg ccccaagag ccggtcgaa 
      aaagtcgaa gaaaataaa acccagaat gatagcgtt 
      cctcagagt gacgaaccc gaggctgac gaaacgctg 
      ataacagct tcgtcagtt gaggaagct ttagaacaa 
      cgactggcc gtgtataag aaacaagaa gagtcggcc 
      aaggaacaa ggcaatgcg agtaaagcc agacgaatg 
      ggtcggatt gtgaaacag tatgagcaa gccatcaag 
      gcgcataaa gcggggaag ccgattcct gttgacgag 
      ttaccgact cctccaggc tatgccccg attccggtt 
      ccgggaagt gaagctccg aaacctaca cccgctgca 
      ccggagcaa ccgcccaga cctaaaaca tctccaaaa 
      acacagccc tccacttca caatcgacg agaatttca 
      ggaaatcat gttccaaat tcacgagcc gacaagcaa 
      ttggttctt cttctcgca aaacagaaa cagttcaaa 
      gaagcggcg cttgccgcc aaacgaaag ggcgaaata 
      gatcaagcc aaggagtac ctaagaact gcgaaaggt 
      tttgacccc cttattgat gcagcgagc tgtggtctt 
      ccggtggat ttatccact ttgccggta tcgccctct 
      gccaagtca caattagac aacgaatac gaaatggtg 
      atggctgac gagtgtagc gaagatgat aactccaac 
      acggacgtc attgcgaga cttgaaaac caattaact 
      aaacagttg aaaatgtgt ctgagtacc cgtgaccac 
      cataaagcc cttggtgat gtcgctggg acaaatcgt 
      tttgaaaga ttggcctta aacgttacg aaagatttg 
      gatattgtg agattagca cggaggact ccaggtgcg 
      ggggttccg aaattccat tacgagaat agagatttt 
      tcaatcgtg aaaagtttc acggaattg ggcgaaaac 
      gatctagaa ttaaccatt gttagaggg ataagctac 
      acttgcagt aatccgaaa gagattgat acgtatgtt 
      aagtttgat tttccatat cctcaggat gaaccgttt 
      agtcaaaga acagccact gtcaaagac acaaacagt 
      ccggaatac aacgccact tacacaata cccatacaa 
      agaaatgca agagtctgc cagagggtg ttcaaaagg 
      cagtctgtc aagtttgag gtttactct aagggcggt 
      ttctttcgt ggagatacg ttaattggt actgccaca 
      gtcaaatta caaccactg gaaactcag tgtgaattg 
      cacgatagt tttgatttg ttggaagga aggaaaaaa 
      attggaggc aaactagaa gtcagaatg aggcttcga 
      catcccatc gttaatcag caagtcgag caattgcat 
      gaaaagtgg cttgttatt gataatgtt taa
    mRNA sequence
    • >TC013686 RA
      tacattaat taaaaattt tcaaatgtt aaccaataa 
      aatcgaaag aagtgacgt ccagttgag tggttgagt 
      catttacga attcaacga acaatttta acaaattaa 
      gttcaaaac gattgtaat gtgaagcca ctatctcat 
      aaacacatt cacagcaat ttgtgtggg ttctaacct 
      tggtaacag ttttgcgtt attgcgtga attcaacaa 
      tgtttgggg ctaagaaaa gcgagaaaa caaagcggg 
      accattccg ggaggaatt tgtcacagt atgggttgt 
      ttgatgtat cagctgatt ttaacctag acggggggc 
      taatggacg gtggtctcg acaatgaga atgatagtg 
      atttggaag ccgaattgg cggcgttga ctcaagggg 
      gcggcggca aacccaaac gtcccccaa ggaagaagg 
      ttcctgatg cggatttag acgcaatgg ttgctgaaa 
      gtatgaagg atatacctt cagacgagg agctgtcag 
      gggatgacg atgatccag aactcttaa atgagctaa 
      gcaacattg cgggagaca cacccgaaa aagaagagg 
      aggatcaac catcgccgg caaggtcac ctgaggggt 
      ccagcgaca tagtgaagc ttttaaatg accgattaa 
      aaatgtacg aagaagcag aaaaaaacg ctaaagctg 
      ccggtgaat caagcaggg caagacgat ttggcagag 
      gcttgaaaa ccctcaagg acttaataa aacaagcga 
      acgccggcc gacccatta acaacgacg acattcctc 
      ccgaagtta gcactggag tgaaaaaac ccccagcca 
      ccgacgaca caaccgatt cgccgccgc agccctcaa 
      gggctgctc ctccagttc ctgtacaat ctgaccccg 
      aacctgaac cagaacccg aaaaaaccc ctgaagaaa 
      caccccaag tagaccccc aactaatcc acttgctta 
      cggaacgaa aaaatcaat acaaaattg ccgccctca 
      aagccaaaa aatcaggcg ataacgcca ctgccatta 
      gttacataa aaatagcca aacagtttg aaacggtca 
      tcgcagcgg cccaaagcg gccaaccag tcgacttat 
      ctcgcatgc caggaccgc cccaagagc cggtcgaaa 
      aagtcgaag aaaataaaa cccagaatg atagcgttc 
      ctcagagtg acgaacccg aggctgacg aaacgctga 
      taacagctt cgtcagttg aggaagctt tagaacaac 
      gactggccg tgtataaga aacaagaag agtcggcca 
      aggaacaag gcaatgcga gtaaagcca gacgaatgg 
      gtcggattg tgaaacagt atgagcaag ccatcaagg 
      cgcataaag cggggaagc cgattcctg ttgacgagt 
      taccgactc ctccaggct atgccccga ttccggttc 
      cgggaagtg aagctccga aacctacac ccgctgcac 
      cggagcaac cgcccagac ctaaaacat ctccaaaaa 
      cacagccct ccacttcac aatcgacga gaatttcag 
      gaaatcatg ttccaaatt cacgagccg acaagcaat 
      tggttcttc ttctcgcaa aacagaaac agttcaaag 
      aagcggcgc ttgccgcca aacgaaagg gcgaaatag 
      atcaagcca aggagtacc taagaactg cgaaaggtt 
      ttgaccccc ttattgatg cagcgagct gtggtcttc 
      cggtggatt tatccactt tgccggtat cgccctctg 
      ccaagtcac aattagaca acgaatacg aaatggtga 
      tggctgacg agtgtagcg aagatgata actccaaca 
      cggacgtca ttgcgagac ttgaaaacc aattaacta 
      aacagttga aaatgtgtc tgagtaccc gtgaccacc 
      ataaagccc ttggtgatg tcgctggga caaatcgtt 
      ttgaaagat tggccttaa acgttacga aagatttgg 
      atattgtga gattagcac ggaggactc caggtgcgg 
      gggttccga aattccatt acgagaata gagattttt 
      caatcgtga aaagtttca cggaattgg gcgaaaacg 
      atctagaat taaccattg ttagaggga taagctaca 
      cttgcagta atccgaaag agattgata cgtatgtta 
      agtttgatt ttccatatc ctcaggatg aaccgttta 
      gtcaaagaa cagccactg tcaaagaca caaacagtc 
      cggaataca acgccactt acacaatac ccatacaaa 
      gaaatgcaa gagtctgcc agagggtgt tcaaaaggc 
      agtctgtca agtttgagg tttactcta agggcggtt 
      tctttcgtg gagatacgt taattggta ctgccacag 
      tcaaattac aaccactgg aaactcagt gtgaattgc 
      acgatagtt ttgatttgt tggaaggaa ggaaaaaaa 
      ttggaggca aactagaag tcagaatga ggcttcgac 
      atcccatcg ttaatcagc aagtcgagc aattgcatg 
      aaaagtggc ttgttattg ataatgttt aaagattaa 
      ttaccttcg tgttgaaaa aagtggaat atattttat 
      tataacgta cgtagcttt aagctagcc ttataatgg 
      tgatgcaat ttgtgtatt taataacca ctgtatttt 
      tgtgatttt ttattatgt actccataa actagatat 
      atctttaaa attgatttt tattttaat aatgttttt 
      gttttcatt aggtttttt tgctcaatg tgcatcaac 
      acttaaggc cccagtcgt gcttcttta gatacatat 
      atttttagt ctgtataat aaacacaga aataaagtt 
      gttgaattt tc